ATE533846T1 - Seprs-mutante und verfahren zur stellengerichteten einführung von phosphoserin in protein unter verwendung davon - Google Patents

Seprs-mutante und verfahren zur stellengerichteten einführung von phosphoserin in protein unter verwendung davon

Info

Publication number
ATE533846T1
ATE533846T1 AT07768165T AT07768165T ATE533846T1 AT E533846 T1 ATE533846 T1 AT E533846T1 AT 07768165 T AT07768165 T AT 07768165T AT 07768165 T AT07768165 T AT 07768165T AT E533846 T1 ATE533846 T1 AT E533846T1
Authority
AT
Austria
Prior art keywords
seprs
mutant
phosphoserine
protein
site
Prior art date
Application number
AT07768165T
Other languages
English (en)
Inventor
Shigeyuki Yokoyama
Ryuya Fukunaga
Shun-Ichi Sekine
Original Assignee
Riken
Univ Tokyo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken, Univ Tokyo filed Critical Riken
Application granted granted Critical
Publication of ATE533846T1 publication Critical patent/ATE533846T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT07768165T 2006-06-28 2007-06-28 Seprs-mutante und verfahren zur stellengerichteten einführung von phosphoserin in protein unter verwendung davon ATE533846T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006178642 2006-06-28
PCT/JP2007/063414 WO2008001947A1 (en) 2006-06-28 2007-06-28 MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME

Publications (1)

Publication Number Publication Date
ATE533846T1 true ATE533846T1 (de) 2011-12-15

Family

ID=38845698

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07768165T ATE533846T1 (de) 2006-06-28 2007-06-28 Seprs-mutante und verfahren zur stellengerichteten einführung von phosphoserin in protein unter verwendung davon

Country Status (5)

Country Link
US (1) US8178301B2 (de)
EP (1) EP2048233B8 (de)
JP (1) JP5305440B2 (de)
AT (1) ATE533846T1 (de)
WO (1) WO2008001947A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101719751B1 (ko) * 2016-04-28 2017-04-04 한국과학기술원 SepRS와 EF-Tu의 변이체를 이용한 인산화 단백질의 제조방법
EP3904568A4 (de) 2018-12-26 2024-03-20 Chugai Seiyaku Kabushiki Kaisha Mutiertes trna für codon-expansion
CN114641569A (zh) 2019-12-26 2022-06-17 中外制药株式会社 翻译用组合物及肽的制备方法
JPWO2021261577A1 (de) 2020-06-25 2021-12-30

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
CA2452818C (en) * 2001-08-01 2010-06-29 Japan Science And Technology Agency Tyrosyl-trna synthetase variants
US7566555B2 (en) * 2002-10-31 2009-07-28 Riken Method of expressing proteins comprising non-naturally-occurring amino acids
JP2006180701A (ja) 2003-02-10 2006-07-13 Institute Of Physical & Chemical Research チロシルｔＲＮＡ合成酵素の変異体及びその作製方法
JP2006178642A (ja) 2004-12-21 2006-07-06 Olympus Corp 動きベクトル検出装置
WO2006107813A2 (en) * 2005-04-05 2006-10-12 Yale University Site specific incorporation of phosphoserine into polypeptides using phosphoseryl-trna synthetase

Also Published As

Publication number Publication date
WO2008001947A1 (en) 2008-01-03
EP2048233A4 (de) 2010-06-30
US8178301B2 (en) 2012-05-15
EP2048233B1 (de) 2011-11-16
JPWO2008001947A1 (ja) 2009-12-03
EP2048233B8 (de) 2012-05-02
US20090233290A1 (en) 2009-09-17
EP2048233A1 (de) 2009-04-15
JP5305440B2 (ja) 2013-10-02

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