ATE534731T1 - Zusammensetzungen und verfahren zur zufallsmutagenese von nukleinsäuren - Google Patents
Zusammensetzungen und verfahren zur zufallsmutagenese von nukleinsäurenInfo
- Publication number
- ATE534731T1 ATE534731T1 AT02746439T AT02746439T ATE534731T1 AT E534731 T1 ATE534731 T1 AT E534731T1 AT 02746439 T AT02746439 T AT 02746439T AT 02746439 T AT02746439 T AT 02746439T AT E534731 T1 ATE534731 T1 AT E534731T1
- Authority
- AT
- Austria
- Prior art keywords
- compositions
- methods
- nucleic acids
- random mutagenesis
- mutagenesis
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 238000002708 random mutagenesis Methods 0.000 title 1
- 238000002703 mutagenesis Methods 0.000 abstract 3
- 231100000350 mutagenesis Toxicity 0.000 abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 101710178665 Error-prone DNA polymerase Proteins 0.000 abstract 1
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
Landscapes
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29434101P | 2001-05-30 | 2001-05-30 | |
| PCT/US2002/016280 WO2002097078A1 (en) | 2001-05-30 | 2002-05-23 | Compositions and methods for random nucleic acid mutagenisis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE534731T1 true ATE534731T1 (de) | 2011-12-15 |
Family
ID=23132994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02746439T ATE534731T1 (de) | 2001-05-30 | 2002-05-23 | Zusammensetzungen und verfahren zur zufallsmutagenese von nukleinsäuren |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US6803216B2 (de) |
| EP (1) | EP1390482B1 (de) |
| JP (1) | JP2005501527A (de) |
| AT (1) | ATE534731T1 (de) |
| CA (1) | CA2448628A1 (de) |
| WO (1) | WO2002097078A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7507376B2 (en) * | 2002-12-19 | 2009-03-24 | 3M Innovative Properties Company | Integrated sample processing devices |
| WO2004072245A2 (en) * | 2003-02-06 | 2004-08-26 | Rensselaer Polytechnic Institute | Polymerase-based protocols for the introductions of deletions and insertions |
| FR2871170B1 (fr) * | 2004-06-07 | 2006-08-11 | Proteus Sa | Procede de determination de la charge mutationnelle d'une banque de genes obtenue par mutagenese aleatoire d'un gene d'interet et les moyens pour sa mise en oeuvre |
| CA2603815C (en) * | 2005-04-15 | 2017-09-26 | Epigenomics Ag | A method for providing dna fragments derived from a remote sample |
| JP5106416B2 (ja) * | 2006-01-06 | 2012-12-26 | アジレント・テクノロジーズ・インク | 濃縮(packed)DNAポリメラーゼを含む核酸複製のための反応緩衝液組成物 |
| GB0620715D0 (en) | 2006-10-18 | 2006-11-29 | Univ Durham | Ethanol production |
| GB201203374D0 (en) | 2012-02-28 | 2012-04-11 | Univ Sheffield | Alkane production |
| CN105283588A (zh) | 2013-02-26 | 2016-01-27 | 艾希奥美公司 | 用于产生用于定向进化的文库的方法 |
| WO2017075421A1 (en) * | 2015-10-29 | 2017-05-04 | Temple University-Of The Commonwealth System Of Higher Education | Modification of 3' terminal ends of nucleic acids by dna polymerase theta |
| GB201602535D0 (en) | 2016-02-12 | 2016-03-30 | Univ Edinburgh | Improved flu vaccine yield |
| GB201802744D0 (en) * | 2018-02-20 | 2018-04-04 | Longas Tech Pty Ltd | Method |
| CN108546698B (zh) * | 2018-04-25 | 2020-03-31 | 浙江华睿生物技术有限公司 | 一种天冬氨酸酶突变体 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545552A (en) * | 1990-12-03 | 1996-08-13 | Stratagene | Purified thermostable pyrococcus furiosus DNA polymerase I |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5556772A (en) | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| US6183997B1 (en) * | 1997-03-21 | 2001-02-06 | Stratagene | Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same |
| JP4245084B2 (ja) * | 1997-03-21 | 2009-03-25 | ストラタジーン | ポリメラーゼ増強因子(pef)抽出物、pefタンパク質複合体、単離されたpefタンパク質、並びに精製及び単離方法 |
| US20030049614A1 (en) * | 1999-10-06 | 2003-03-13 | Holly Hurlbut Hogrefe | Compositions for dna amplification, synthesis, and mutagenesis |
| US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
-
2002
- 2002-05-23 JP JP2003500245A patent/JP2005501527A/ja active Pending
- 2002-05-23 AT AT02746439T patent/ATE534731T1/de active
- 2002-05-23 CA CA002448628A patent/CA2448628A1/en not_active Abandoned
- 2002-05-23 WO PCT/US2002/016280 patent/WO2002097078A1/en not_active Ceased
- 2002-05-23 EP EP02746439A patent/EP1390482B1/de not_active Expired - Lifetime
- 2002-05-23 US US10/154,206 patent/US6803216B2/en not_active Expired - Lifetime
-
2004
- 2004-09-17 US US10/943,511 patent/US20050032177A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2448628A1 (en) | 2002-12-05 |
| US20030152944A1 (en) | 2003-08-14 |
| JP2005501527A (ja) | 2005-01-20 |
| EP1390482A4 (de) | 2005-01-19 |
| EP1390482A1 (de) | 2004-02-25 |
| US6803216B2 (en) | 2004-10-12 |
| US20050032177A1 (en) | 2005-02-10 |
| WO2002097078A1 (en) | 2002-12-05 |
| EP1390482B1 (de) | 2011-11-23 |
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