ATE540967T1 - Verfahren zur reinigung von proteinen - Google Patents
Verfahren zur reinigung von proteinenInfo
- Publication number
- ATE540967T1 ATE540967T1 AT04778787T AT04778787T ATE540967T1 AT E540967 T1 ATE540967 T1 AT E540967T1 AT 04778787 T AT04778787 T AT 04778787T AT 04778787 T AT04778787 T AT 04778787T AT E540967 T1 ATE540967 T1 AT E540967T1
- Authority
- AT
- Austria
- Prior art keywords
- salt
- protein
- column
- purifying proteins
- dynamic capacity
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 150000003839 salts Chemical class 0.000 abstract 5
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
- 230000002535 lyotropic effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54058704P | 2004-01-30 | 2004-01-30 | |
| PCT/US2004/023434 WO2005075498A1 (en) | 2004-01-30 | 2004-07-21 | Process for purifying proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE540967T1 true ATE540967T1 (de) | 2012-01-15 |
Family
ID=34837404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04778787T ATE540967T1 (de) | 2004-01-30 | 2004-07-21 | Verfahren zur reinigung von proteinen |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US7781395B2 (de) |
| EP (1) | EP1711512B1 (de) |
| AT (1) | ATE540967T1 (de) |
| AU (1) | AU2004315196B2 (de) |
| CA (1) | CA2554929A1 (de) |
| ES (1) | ES2377125T3 (de) |
| WO (1) | WO2005075498A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3366692A1 (de) | 2009-06-22 | 2018-08-29 | Amgen, Inc | Proteinneufaltung mit einem chemisch gesteuerten redoxzustand |
| AU2010266093B2 (en) | 2009-06-25 | 2013-06-27 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| SG191186A1 (en) | 2010-12-15 | 2013-07-31 | Baxter Int | Eluate collection using conductivity gradient |
| CN102675465A (zh) * | 2011-03-09 | 2012-09-19 | 上海赛金生物医药有限公司 | 重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白的纯化方法 |
| KR101454316B1 (ko) * | 2011-08-17 | 2014-10-27 | 한화케미칼 주식회사 | 활성형 TNFR-Fc 융합 단백질을 제조하는 방법 |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| JP6592426B2 (ja) * | 2013-03-15 | 2019-10-16 | バイオジェン・エムエイ・インコーポレイテッドBiogen MA Inc. | 無塩条件下で疎水性相互作用クロマトグラフィーを使用するタンパク質精製 |
| KR20230124578A (ko) | 2020-12-22 | 2023-08-25 | 암젠 인크 | 세포 배양 방법 |
| CN112574321B (zh) * | 2020-12-30 | 2023-10-20 | 上海赛金生物医药有限公司 | 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5231178A (en) * | 1991-01-16 | 1993-07-27 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Method for the purification of intact, correctly-folded insulin-like growth factor-1 |
| US5395856A (en) * | 1991-05-16 | 1995-03-07 | Rohm And Haas Company | HPLC avidin monomer affinity resin |
| DE69415669T2 (de) * | 1993-05-17 | 1999-07-01 | Genentech, Inc., South San Francisco, Calif. | Sialidase defiziente zellen |
-
2004
- 2004-07-21 EP EP04778787A patent/EP1711512B1/de not_active Revoked
- 2004-07-21 US US10/895,581 patent/US7781395B2/en not_active Expired - Lifetime
- 2004-07-21 AU AU2004315196A patent/AU2004315196B2/en not_active Ceased
- 2004-07-21 CA CA002554929A patent/CA2554929A1/en not_active Abandoned
- 2004-07-21 AT AT04778787T patent/ATE540967T1/de active
- 2004-07-21 WO PCT/US2004/023434 patent/WO2005075498A1/en not_active Ceased
- 2004-07-21 ES ES04778787T patent/ES2377125T3/es not_active Expired - Lifetime
-
2010
- 2010-06-23 US US12/822,072 patent/US8273707B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1711512B1 (de) | 2012-01-11 |
| ES2377125T3 (es) | 2012-03-22 |
| CA2554929A1 (en) | 2005-08-18 |
| US20100311953A1 (en) | 2010-12-09 |
| WO2005075498A1 (en) | 2005-08-18 |
| US8273707B2 (en) | 2012-09-25 |
| US20060229437A1 (en) | 2006-10-12 |
| EP1711512A1 (de) | 2006-10-18 |
| AU2004315196B2 (en) | 2008-10-02 |
| US7781395B2 (en) | 2010-08-24 |
| AU2004315196A1 (en) | 2005-08-18 |
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