ATE541041T1 - Produktion von rekombinantem aav mit hohem titer - Google Patents
Produktion von rekombinantem aav mit hohem titerInfo
- Publication number
- ATE541041T1 ATE541041T1 AT03754869T AT03754869T ATE541041T1 AT E541041 T1 ATE541041 T1 AT E541041T1 AT 03754869 T AT03754869 T AT 03754869T AT 03754869 T AT03754869 T AT 03754869T AT E541041 T1 ATE541041 T1 AT E541041T1
- Authority
- AT
- Austria
- Prior art keywords
- production
- recombinant aav
- raav
- high titre
- titre recombinant
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 2
- 241000702421 Dependoparvovirus Species 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 241001529453 unidentified herpesvirus Species 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/252,182 US7091029B2 (en) | 2002-09-23 | 2002-09-23 | High titer recombinant AAV production |
| PCT/US2003/030098 WO2004027048A2 (en) | 2002-09-23 | 2003-09-23 | High titer recombinant aav production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE541041T1 true ATE541041T1 (de) | 2012-01-15 |
Family
ID=31992891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03754869T ATE541041T1 (de) | 2002-09-23 | 2003-09-23 | Produktion von rekombinantem aav mit hohem titer |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7091029B2 (de) |
| EP (1) | EP1546343B1 (de) |
| AT (1) | ATE541041T1 (de) |
| AU (1) | AU2003272672B2 (de) |
| CA (1) | CA2499386C (de) |
| ES (1) | ES2378883T3 (de) |
| NZ (1) | NZ562881A (de) |
| WO (1) | WO2004027048A2 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070202587A1 (en) * | 2002-09-23 | 2007-08-30 | Applied Genetic Technologies Corporation | Recombinant AAV production in mammalian cells |
| AU2009209408B2 (en) | 2008-01-29 | 2015-06-11 | Beacon Therapeutics Limited | Recombinant virus production using mammalian cells in suspension |
| DK2943567T3 (en) | 2013-01-08 | 2018-01-02 | Genzyme Corp | USE OF INOS INHIBITORS TO INCREASE VIRAL YIELD IN CULTURE |
| WO2015031686A1 (en) | 2013-08-30 | 2015-03-05 | Amgen Inc. | High titer recombinant aav vector production in adherent and suspension cells |
| US9534225B2 (en) * | 2014-04-15 | 2017-01-03 | Applied Genetic Technologies Corporation | Codon optimized nucleic acid encoding a retinitis pigmentosa gtpase regulator (RPGR) |
| WO2015173926A1 (ja) * | 2014-05-15 | 2015-11-19 | 高砂電気工業株式会社 | マイクロ蠕動ポンプ |
| EP3242945B1 (de) | 2015-01-07 | 2021-09-01 | Universitat Autònoma de Barcelona | Einzelvektor-genkonstrukt mit insulin- und glucokinasegenen |
| US20190256867A1 (en) | 2018-02-01 | 2019-08-22 | Homology Medicines, Inc. | Adeno-associated virus compositions for restoring pah gene function and methods of use thereof |
| US10610606B2 (en) | 2018-02-01 | 2020-04-07 | Homology Medicines, Inc. | Adeno-associated virus compositions for PAH gene transfer and methods of use thereof |
| CN112041437A (zh) | 2018-02-19 | 2020-12-04 | 同源药物公司 | 用于恢复f8基因功能的腺相关病毒组合物和其使用方法 |
| GB201806333D0 (en) * | 2018-04-18 | 2018-05-30 | Glaxosmithkline Ip Dev Ltd | Parvovirus vector production |
| EP3914696A4 (de) * | 2019-01-25 | 2022-12-14 | Biogen MA Inc. | Saatkulturverfahren zur aav-produktion |
| SG11202110607WA (en) | 2019-04-01 | 2021-10-28 | Tenaya Therapeutics Inc | Adeno-associated virus with engineered capsid |
| US20220307055A1 (en) * | 2019-08-28 | 2022-09-29 | University Of Florida Research Foundation, Incorporated | Improved production of recombinant aav using embryonated avian eggs |
| TW202140791A (zh) | 2020-01-13 | 2021-11-01 | 美商霍蒙拉奇醫藥公司 | 治療苯酮尿症之方法 |
| TW202208632A (zh) | 2020-05-27 | 2022-03-01 | 美商同源醫藥公司 | 用於恢復ｐａｈ基因功能的腺相關病毒組成物及其使用方法 |
| CN115698304A (zh) | 2020-10-09 | 2023-02-03 | 特纳亚治疗股份有限公司 | 亲斑蛋白-2基因疗法的方法和组合物 |
| US20230407329A1 (en) | 2020-11-20 | 2023-12-21 | University Of Florida Research Foundation, Incorporated | Improved aav production using potassium chloride |
| AU2022267891A1 (en) * | 2021-04-27 | 2023-11-09 | Novartis Ag | Viral vector production system |
| EP4508062A1 (de) | 2022-04-11 | 2025-02-19 | Tenaya Therapeutics, Inc. | Adeno-assoziiertes virus mit manipuliertem kapsid |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2626383B1 (fr) * | 1988-01-27 | 1991-10-25 | Commissariat Energie Atomique | Procede de microscopie optique confocale a balayage et en profondeur de champ etendue et dispositifs pour la mise en oeuvre du procede |
| US6686200B1 (en) * | 1993-08-31 | 2004-02-03 | Uab Research Foundation | Methods and compositions for the large scale production of recombinant adeno-associated virus |
| US5965441A (en) | 1996-11-13 | 1999-10-12 | The General Hospital Coporation | HSV/AAV hybrid amplicon vectors |
| GB9721909D0 (en) * | 1997-10-17 | 1997-12-17 | Cantab Pharma Res | Gene delivery vectors and their uses |
| US7258858B2 (en) * | 1997-12-24 | 2007-08-21 | University Of Guelph | Method of identifying high immune response animals |
| WO2000011149A1 (en) * | 1998-08-24 | 2000-03-02 | Uab Research Foundation | Methods of producing high titer recombinant adeno-associated virus |
| AU6151899A (en) * | 1998-09-23 | 2000-04-10 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Methods of treating chronic pain |
| WO2001066782A1 (en) * | 2000-03-03 | 2001-09-13 | The Board Of Trustees Of The Leland Stanford Junior University | Adeno-associated viral compositions and methods |
-
2002
- 2002-09-23 US US10/252,182 patent/US7091029B2/en not_active Expired - Lifetime
-
2003
- 2003-09-23 ES ES03754869T patent/ES2378883T3/es not_active Expired - Lifetime
- 2003-09-23 CA CA2499386A patent/CA2499386C/en not_active Expired - Lifetime
- 2003-09-23 EP EP03754869A patent/EP1546343B1/de not_active Expired - Lifetime
- 2003-09-23 NZ NZ562881A patent/NZ562881A/en not_active IP Right Cessation
- 2003-09-23 AU AU2003272672A patent/AU2003272672B2/en not_active Expired
- 2003-09-23 WO PCT/US2003/030098 patent/WO2004027048A2/en not_active Ceased
- 2003-09-23 AT AT03754869T patent/ATE541041T1/de active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2378883T3 (es) | 2012-04-18 |
| CA2499386A1 (en) | 2004-04-01 |
| EP1546343A2 (de) | 2005-06-29 |
| AU2003272672A1 (en) | 2004-04-08 |
| US7091029B2 (en) | 2006-08-15 |
| NZ562881A (en) | 2009-07-31 |
| AU2003272672B2 (en) | 2006-04-06 |
| EP1546343A4 (de) | 2007-12-19 |
| CA2499386C (en) | 2014-12-09 |
| US20040058439A1 (en) | 2004-03-25 |
| WO2004027048A3 (en) | 2004-07-15 |
| WO2004027048A2 (en) | 2004-04-01 |
| EP1546343B1 (de) | 2012-01-11 |
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