ATE541041T1 - Produktion von rekombinantem aav mit hohem titer - Google Patents
Produktion von rekombinantem aav mit hohem titerInfo
- Publication number
- ATE541041T1 ATE541041T1 AT03754869T AT03754869T ATE541041T1 AT E541041 T1 ATE541041 T1 AT E541041T1 AT 03754869 T AT03754869 T AT 03754869T AT 03754869 T AT03754869 T AT 03754869T AT E541041 T1 ATE541041 T1 AT E541041T1
- Authority
- AT
- Austria
- Prior art keywords
- production
- recombinant aav
- raav
- high titre
- titre recombinant
- Prior art date
Links
- 238000000034 method Methods 0.000 abstract 2
- 241000702421 Dependoparvovirus Species 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 241001529453 unidentified herpesvirus Species 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/252,182 US7091029B2 (en) | 2002-09-23 | 2002-09-23 | High titer recombinant AAV production |
| PCT/US2003/030098 WO2004027048A2 (en) | 2002-09-23 | 2003-09-23 | High titer recombinant aav production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE541041T1 true ATE541041T1 (de) | 2012-01-15 |
Family
ID=31992891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03754869T ATE541041T1 (de) | 2002-09-23 | 2003-09-23 | Produktion von rekombinantem aav mit hohem titer |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7091029B2 (de) |
| EP (1) | EP1546343B1 (de) |
| AT (1) | ATE541041T1 (de) |
| AU (1) | AU2003272672B2 (de) |
| CA (1) | CA2499386C (de) |
| ES (1) | ES2378883T3 (de) |
| NZ (1) | NZ562881A (de) |
| WO (1) | WO2004027048A2 (de) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070202587A1 (en) * | 2002-09-23 | 2007-08-30 | Applied Genetic Technologies Corporation | Recombinant AAV production in mammalian cells |
| ES2751999T3 (es) | 2008-01-29 | 2020-04-02 | Applied Genetic Tech Corporation | Producción recombinante de virus adeno-asociados usando células BHK en suspensión |
| EP3521420A1 (de) | 2013-01-08 | 2019-08-07 | Genzyme Corporation | Verwendung von inos-inhibitoren zur erhöhung der viralen ausbeute in kulturen |
| WO2015031686A1 (en) * | 2013-08-30 | 2015-03-05 | Amgen Inc. | High titer recombinant aav vector production in adherent and suspension cells |
| US9534225B2 (en) * | 2014-04-15 | 2017-01-03 | Applied Genetic Technologies Corporation | Codon optimized nucleic acid encoding a retinitis pigmentosa gtpase regulator (RPGR) |
| US20170058881A1 (en) * | 2014-05-15 | 2017-03-02 | Takasago Electric, Inc. | Micro peristaltic pump |
| US11033638B2 (en) | 2015-01-07 | 2021-06-15 | Universität Autonoma De Barcelona | Single-vector gene construct comprising insulin and glucokinase genes |
| US20190256867A1 (en) | 2018-02-01 | 2019-08-22 | Homology Medicines, Inc. | Adeno-associated virus compositions for restoring pah gene function and methods of use thereof |
| US10610606B2 (en) | 2018-02-01 | 2020-04-07 | Homology Medicines, Inc. | Adeno-associated virus compositions for PAH gene transfer and methods of use thereof |
| JP7244547B2 (ja) | 2018-02-19 | 2023-03-22 | ホモロジー・メディシンズ・インコーポレイテッド | Ｆ８遺伝子機能を回復させるためのアデノ随伴ウイルス組成物及びその使用の方法 |
| GB201806333D0 (en) * | 2018-04-18 | 2018-05-30 | Glaxosmithkline Ip Dev Ltd | Parvovirus vector production |
| CA3127629A1 (en) * | 2019-01-25 | 2020-07-30 | Biogen Ma Inc. | Seed culture process for aav production |
| EP3947700A4 (de) | 2019-04-01 | 2023-01-04 | Tenaya Therapeutics, Inc. | Adeno-assoziiertes virus mit manipuliertem kapsid |
| EP4021469A4 (de) * | 2019-08-28 | 2023-10-04 | University Of Florida Research Foundation, Incorporated | Verbesserte herstellung von rekombinantem aav unter verwendung von embryonierten vogeleiern |
| TW202140791A (zh) | 2020-01-13 | 2021-11-01 | 美商霍蒙拉奇醫藥公司 | 治療苯酮尿症之方法 |
| TW202208632A (zh) | 2020-05-27 | 2022-03-01 | 美商同源醫藥公司 | 用於恢復ｐａｈ基因功能的腺相關病毒組成物及其使用方法 |
| BR112023006305A2 (pt) | 2020-10-09 | 2023-05-09 | Tenaya Therapeutics Inc | Métodos e composições de terapia gênica com placofilina-2 |
| WO2022109241A1 (en) | 2020-11-20 | 2022-05-27 | University Of Florida Research Foundation, Incorporated | Improved aav production using potassium chloride |
| WO2022229853A1 (en) * | 2021-04-27 | 2022-11-03 | Novartis Ag | Viral vector production system |
| WO2023201207A1 (en) | 2022-04-11 | 2023-10-19 | Tenaya Therapeutics, Inc. | Adeno-associated virus with engineered capsid |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2626383B1 (fr) * | 1988-01-27 | 1991-10-25 | Commissariat Energie Atomique | Procede de microscopie optique confocale a balayage et en profondeur de champ etendue et dispositifs pour la mise en oeuvre du procede |
| US6686200B1 (en) * | 1993-08-31 | 2004-02-03 | Uab Research Foundation | Methods and compositions for the large scale production of recombinant adeno-associated virus |
| US5965441A (en) | 1996-11-13 | 1999-10-12 | The General Hospital Coporation | HSV/AAV hybrid amplicon vectors |
| GB9721909D0 (en) * | 1997-10-17 | 1997-12-17 | Cantab Pharma Res | Gene delivery vectors and their uses |
| US7258858B2 (en) * | 1997-12-24 | 2007-08-21 | University Of Guelph | Method of identifying high immune response animals |
| AU5689699A (en) * | 1998-08-24 | 2000-03-14 | Uab Research Foundation | Methods of producing high titer recombinant adeno-associated virus |
| WO2000016799A1 (en) * | 1998-09-23 | 2000-03-30 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Methods of treating chronic pain |
| AU2001240018A1 (en) * | 2000-03-03 | 2001-09-17 | The Board Of Trustees Of The Leland Stanford Junior University | Adeno-associated viral compositions and methods |
-
2002
- 2002-09-23 US US10/252,182 patent/US7091029B2/en not_active Expired - Lifetime
-
2003
- 2003-09-23 AU AU2003272672A patent/AU2003272672B2/en not_active Expired
- 2003-09-23 CA CA2499386A patent/CA2499386C/en not_active Expired - Lifetime
- 2003-09-23 NZ NZ562881A patent/NZ562881A/en not_active IP Right Cessation
- 2003-09-23 WO PCT/US2003/030098 patent/WO2004027048A2/en not_active Ceased
- 2003-09-23 AT AT03754869T patent/ATE541041T1/de active
- 2003-09-23 ES ES03754869T patent/ES2378883T3/es not_active Expired - Lifetime
- 2003-09-23 EP EP03754869A patent/EP1546343B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2378883T3 (es) | 2012-04-18 |
| AU2003272672A1 (en) | 2004-04-08 |
| NZ562881A (en) | 2009-07-31 |
| EP1546343A2 (de) | 2005-06-29 |
| EP1546343B1 (de) | 2012-01-11 |
| CA2499386A1 (en) | 2004-04-01 |
| US7091029B2 (en) | 2006-08-15 |
| WO2004027048A2 (en) | 2004-04-01 |
| WO2004027048A3 (en) | 2004-07-15 |
| EP1546343A4 (de) | 2007-12-19 |
| AU2003272672B2 (en) | 2006-04-06 |
| CA2499386C (en) | 2014-12-09 |
| US20040058439A1 (en) | 2004-03-25 |
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