ATE546228T1 - Verfahren und vorrichtung zur handhabung einzelner zellen und kleiner aggregate davon - Google Patents

Verfahren und vorrichtung zur handhabung einzelner zellen und kleiner aggregate davon

Info

Publication number
ATE546228T1
ATE546228T1 AT07734717T AT07734717T ATE546228T1 AT E546228 T1 ATE546228 T1 AT E546228T1 AT 07734717 T AT07734717 T AT 07734717T AT 07734717 T AT07734717 T AT 07734717T AT E546228 T1 ATE546228 T1 AT E546228T1
Authority
AT
Austria
Prior art keywords
individual cells
well
small aggregates
handling individual
liquid
Prior art date
Application number
AT07734717T
Other languages
English (en)
Inventor
Roberto Guerrieri
Massimo Bocchi
Original Assignee
Mindseeds Lab S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mindseeds Lab S R L filed Critical Mindseeds Lab S R L
Application granted granted Critical
Publication of ATE546228T1 publication Critical patent/ATE546228T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT07734717T 2006-05-31 2007-05-31 Verfahren und vorrichtung zur handhabung einzelner zellen und kleiner aggregate davon ATE546228T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT001063A ITMI20061063A1 (it) 2006-05-31 2006-05-31 Metrodo e apparato pe rla selezione e la modifica di singole cellule e loro piccoli aggregati
PCT/IB2007/001427 WO2007138464A2 (en) 2006-05-31 2007-05-31 Method and apparatus for manipulating single cells and small aggregates thereof

Publications (1)

Publication Number Publication Date
ATE546228T1 true ATE546228T1 (de) 2012-03-15

Family

ID=38657970

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07734717T ATE546228T1 (de) 2006-05-31 2007-05-31 Verfahren und vorrichtung zur handhabung einzelner zellen und kleiner aggregate davon

Country Status (7)

Country Link
US (2) US9039883B2 (de)
EP (1) EP2032262B3 (de)
JP (1) JP2009539096A (de)
CN (1) CN101460253B (de)
AT (1) ATE546228T1 (de)
IT (1) ITMI20061063A1 (de)
WO (1) WO2007138464A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITBO20050481A1 (it) 2005-07-19 2007-01-20 Silicon Biosystems S R L Metodo ed apparato per la manipolazione e/o l'individuazione di particelle
ITTO20060226A1 (it) 2006-03-27 2007-09-28 Silicon Biosystem S P A Metodo ed apparato per il processamento e o l'analisi e o la selezione di particelle, in particolare particelle biologiche
WO2010037085A1 (en) 2008-09-29 2010-04-01 The Board Of Trustees Of The University Of Illinois Dna sequencing and amplification systems using nanoscale field effect sensor arrays
US20110192726A1 (en) * 2008-10-31 2011-08-11 Agency For Science ,Technology And Research Device and method for detection of analyte from a sample
IT1391619B1 (it) * 2008-11-04 2012-01-11 Silicon Biosystems Spa Metodo per l'individuazione, selezione e analisi di cellule tumorali
US10895575B2 (en) * 2008-11-04 2021-01-19 Menarini Silicon Biosystems S.P.A. Method for identification, selection and analysis of tumour cells
CN101738463A (zh) * 2008-11-05 2010-06-16 中国科学院电子学研究所 一种集富集检测功能的微电极生物传感芯片
FR2956207B1 (fr) * 2010-02-10 2012-05-04 Horiba Abx Sas Dispositif et procede de mesures multiparametriques de microparticules dans un fluide
US9250113B2 (en) 2010-06-21 2016-02-02 The Board Of Trustees Of The University Of Illinois Cell mass measurement and apparatus
EP2646830B1 (de) 2010-12-03 2016-04-13 Cellply S.R.L. Schneller screening von monoklonalantikörpern
EP3035031B1 (de) * 2010-12-03 2022-06-01 Cellply S.R.L. Mikroanalyse von zellulären funktionen
MY179484A (en) * 2011-08-24 2020-11-08 Mimos Bhd Apparatus for sorting particles by dielectrophoresis
ITTO20110990A1 (it) 2011-10-28 2013-04-29 Silicon Biosystems Spa Metodo ed apparato per l'analisi ottica di particelle a basse temperature
ITBO20110766A1 (it) 2011-12-28 2013-06-29 Silicon Biosystems Spa Dispositivi, apparato, kit e metodo per il trattamento di un campione biologico
CA2868261C (en) 2012-03-27 2020-07-14 The Regents Of The University Of California Continuous whole-chip 3-dimensional dep cell sorter and related fabrication method
JP6233889B2 (ja) * 2012-06-18 2017-11-22 国立研究開発法人理化学研究所 試料分注装置、およびタンパク質の結晶化方法
HK1216552A1 (zh) 2012-10-15 2016-11-18 纳诺赛莱克特生物医药股份有限公司 顆粒分選的系統、設備和方法
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
EP3164482B1 (de) 2014-07-03 2024-05-22 Massachusetts Institute of Technology Vorrichtung und verfahren zur optimierung der zellelektroporation
CN104789468B (zh) * 2014-07-22 2017-10-20 奥克莱流体公司 颗粒筛选装置
SG11201703493SA (en) 2014-10-29 2017-05-30 Corning Inc Cell culture insert
CN107109339B (zh) 2014-10-29 2021-07-02 康宁股份有限公司 灌注生物反应器平台
EP3212761A1 (de) 2014-10-29 2017-09-06 Corning Incorporated Mikrovertiefungsentwurf und -herstellung zur erzeugung von zellkulturaggregaten
US10385306B2 (en) 2014-12-18 2019-08-20 Unist(Ulsan National Institute Of Science And Technology) Device and method for single cell screening based on inter-cellular communication
CN105505772B (zh) * 2015-12-07 2018-07-10 中国科学院苏州生物医学工程技术研究所 对单个外泌体进行裂解的装置
RU2739327C2 (ru) * 2016-06-14 2020-12-23 Селплай С.Р.Л. Набор и способ скрининга
IT201600122158A1 (it) * 2016-12-01 2018-06-01 Cellply S R L Kit e metodo per l'immissione di uno o piu' fluidi in un dispositivo microfluidico
WO2018031686A1 (en) * 2016-08-09 2018-02-15 The Regents Of The University Of California A device for massively parallel high throughput single cell electroporation and uses thereof
CN107058075B (zh) * 2017-06-20 2023-10-20 商丘师范学院 一种植物细胞原生质体纯化仪及纯化方法
JP7195302B2 (ja) 2017-07-14 2022-12-23 コーニング インコーポレイテッド ３ｄ培養のための細胞培養容器及び３ｄ細胞の培養方法
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
US11767499B2 (en) 2017-07-14 2023-09-26 Corning Incorporated Cell culture vessel
PL3652290T3 (pl) 2017-07-14 2022-08-22 Corning Incorporated Naczynia do hodowli komórkowej 3d do ręcznej lub automatycznej zmiany pożywek
JP6742618B2 (ja) * 2018-06-11 2020-08-19 シャープ株式会社 生体粒子観察装置および生体粒子観察方法
US11912968B2 (en) 2018-07-13 2024-02-27 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
WO2020013845A1 (en) 2018-07-13 2020-01-16 Corning Incorporated Cell culture vessels with stabilizer devices
CN111065725B (zh) 2018-07-13 2024-03-29 康宁股份有限公司 包括具有互联的壁的微板的流体装置
EP3873668B1 (de) * 2018-11-02 2024-10-30 Neofluidics, LLC Mikrofluidische viskosimeteranordnung und verfahren zur verwendung dieser
KR20240097974A (ko) * 2019-04-23 2024-06-27 미코노스 인코포레이티드 인터페이싱을 위해 공동에 근접한 입자의 유전영동 고정화
US11674919B2 (en) * 2019-07-17 2023-06-13 Taiwan Semiconductor Manufacturing Company Ltd. Detector, detection device and method of using the same
KR102254587B1 (ko) 2020-06-12 2021-05-21 한국과학기술연구원 고효율 및 고민감도 입자 포집형 테라헤르츠 센싱 시스템
EP3943924A1 (de) 2020-07-22 2022-01-26 Technische Universität München Mikrotiterplatte für impedanzmessungen an zellclustern
CN112094742B (zh) * 2020-08-21 2022-07-12 北京化工大学 细胞电穿孔转染与活细胞分选同步实现的微流控芯片
CN112051252A (zh) * 2020-09-16 2020-12-08 中国科学院长春应用化学研究所 一种样品池及其制备方法与应用
KR102799481B1 (ko) * 2023-01-09 2025-04-23 한국화학연구원 세포 스페로이드 임피던스 측정 장치를 위한 측정 플레이트

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4747919A (en) * 1987-01-16 1988-05-31 Large Scale Biology Corporation Apparatus and method for electrophoresis in tubes
JP3104995B2 (ja) * 1991-01-31 2000-10-30 ワーナー−ランバート・コンパニー 抗炎症剤として有用な４，６−ジ−第三ブチル−５−ヒドロキシ−１，３−ピリミジンの置換されたヘテロアリール類似体
US5814668A (en) * 1995-09-29 1998-09-29 Buckman Laboratories International Inc. Methods and compositions for controlling biofouling using amides
US20020053399A1 (en) * 1996-07-30 2002-05-09 Aclara Biosciences, Inc Methods for fabricating enclosed microchannel structures
GB9719673D0 (en) * 1997-09-17 1997-11-19 Glaxo Group Ltd Novel apparatus
US20020092767A1 (en) * 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
AU785167B2 (en) 1998-05-29 2006-10-12 Industrial Research Limited Method and apparatus for concentrating and/or positioning particles or cells
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US7198702B1 (en) * 1999-09-30 2007-04-03 Wako Pure Chemical Industries, Ltd. Method for separating substances using dielectrophoretic forces
ATE370793T1 (de) * 2000-04-13 2007-09-15 Wako Pure Chem Ind Ltd Elektroden-bau für dielektrophoretische anordnung und dielektrophoretische trennung
AU8470001A (en) * 2000-08-03 2002-02-18 Caliper Techn Corp Methods and devices for high throughput fluid delivery
US6932893B2 (en) * 2000-10-02 2005-08-23 Sophion Bioscience A/S System for electrophysiological measurements
US20060029955A1 (en) * 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
CA2441366A1 (en) * 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
AT5044U1 (de) * 2001-05-10 2002-02-25 Medsystems Diagnostics Gmbh Quantitativer einschritt immuntest in lyophilisierter form
US20040011652A1 (en) * 2002-07-16 2004-01-22 Bressler Vincent Edward Separation of particles using multiple conductive layers
US7189578B1 (en) * 2002-12-02 2007-03-13 Cfd Research Corporation Methods and systems employing electrothermally induced flow for mixing and cleaning in microsystems
GB2398751A (en) * 2003-02-28 2004-09-01 Univ Surrey A dielectrophoretic separation device
KR100624460B1 (ko) * 2005-02-12 2006-09-19 삼성전자주식회사 나노 내지 마이크로 크기의 포어가 형성되어 있는 막을 포함하는 미세유동장치 및 그를 이용하여 분극성 물질을 분리하는 방법
WO2015116591A1 (en) * 2014-01-30 2015-08-06 Illumina, Inc. Compositions and methods for dispensing reagents

Also Published As

Publication number Publication date
EP2032262B3 (de) 2015-12-02
WO2007138464A3 (en) 2008-02-14
EP2032262A2 (de) 2009-03-11
US20150224496A1 (en) 2015-08-13
ITMI20061063A1 (it) 2007-12-01
EP2032262B9 (de) 2012-08-01
US9039883B2 (en) 2015-05-26
WO2007138464A2 (en) 2007-12-06
CN101460253A (zh) 2009-06-17
CN101460253B (zh) 2012-10-24
US20090288963A1 (en) 2009-11-26
EP2032262B1 (de) 2012-02-22
JP2009539096A (ja) 2009-11-12

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