ATE550099T1 - Verbessertes system für mehrfarbige echtzeit-pcr - Google Patents

Verbessertes system für mehrfarbige echtzeit-pcr

Info

Publication number
ATE550099T1
ATE550099T1 AT04725021T AT04725021T ATE550099T1 AT E550099 T1 ATE550099 T1 AT E550099T1 AT 04725021 T AT04725021 T AT 04725021T AT 04725021 T AT04725021 T AT 04725021T AT E550099 T1 ATE550099 T1 AT E550099T1
Authority
AT
Austria
Prior art keywords
time pcr
fret
improved system
color real
directed
Prior art date
Application number
AT04725021T
Other languages
English (en)
Inventor
Gregor Sagner
Ingrid Bechler
Joachim Bolte
Dieter Heindl
Hans-Peter Josel
Martin Gutekunst
Rudolf Seibl
Christoph Mueller
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE550099T1 publication Critical patent/ATE550099T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT04725021T 2003-04-04 2004-04-01 Verbessertes system für mehrfarbige echtzeit-pcr ATE550099T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP03007458 2003-04-04
EP03014929 2003-07-01
EP03017561 2003-08-07
PCT/EP2004/003457 WO2004087950A2 (en) 2003-04-04 2004-04-01 Improved system for multi color real time pcr

Publications (1)

Publication Number Publication Date
ATE550099T1 true ATE550099T1 (de) 2012-04-15

Family

ID=33135519

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04725021T ATE550099T1 (de) 2003-04-04 2004-04-01 Verbessertes system für mehrfarbige echtzeit-pcr

Country Status (7)

Country Link
US (2) US8137616B2 (de)
EP (2) EP2284278A1 (de)
JP (2) JP2006521092A (de)
AT (1) ATE550099T1 (de)
CA (1) CA2525482C (de)
ES (1) ES2384170T3 (de)
WO (1) WO2004087950A2 (de)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
WO2004087950A2 (en) 2003-04-04 2004-10-14 Roche Diagnostics Gmbh Improved system for multi color real time pcr
EP2348320B1 (de) * 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7709249B2 (en) 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7527763B2 (en) 2005-07-05 2009-05-05 3M Innovative Properties Company Valve control system for a rotating multiplex fluorescence detection device
EP1746169B1 (de) * 2005-07-21 2009-12-23 Epigenomics AG Verfahren zur Quantifizierung methylierter DNA
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8404438B2 (en) 2006-11-30 2013-03-26 Arkray, Inc. Probes for detection of SULT1A1 gene, reagent containing the same, and the uses thereof
CA2722484A1 (en) 2008-04-24 2009-10-29 Peter D. Ludowise Analysis of nucleic acid amplification curves using wavelet transformation
JP2012505644A (ja) * 2008-10-15 2012-03-08 エフ．ホフマン−ラ ロシュ アーゲー オリゴヌクレオチド検出法
JP2012506705A (ja) 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
CN102625850B (zh) * 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
JP2012524242A (ja) * 2009-04-15 2012-10-11 ビオカルティ ソシエテ アノニム Ｐｃｒ反応をリアルタイムで監視するための光検出システム
CN102414327B (zh) 2009-05-01 2015-04-08 奇亚根盖瑟斯堡股份有限公司 用于在样品中检测rna剪接形式的非靶向扩增方法
KR20120031952A (ko) * 2009-05-29 2012-04-04 사이시스 다이애그노스틱스 개선된 병원체 검출 및 스크리닝
WO2011032124A1 (en) 2009-09-14 2011-03-17 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
JP5709897B2 (ja) * 2009-12-24 2015-04-30 シージーン アイエヌシー 偽シグナルが排除されるターゲット核酸配列のリアルタイムマルチプレッキシング検出
WO2011082415A2 (en) 2010-01-04 2011-07-07 Qiagen Gaithersburg, Inc. Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples
JP6103941B2 (ja) 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド 核酸の配列特異的精製および多重分析のための方法および組成物
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
BR112012021202B1 (pt) 2010-02-23 2020-06-09 Genturadx Usa Inc aparelho e métodos para preparação, reação e detecção integradas de amostras
CA2799200A1 (en) * 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
GB201020827D0 (en) * 2010-12-08 2011-01-19 Fotech Solutions Ltd Distrubuted optical fibre sensor
IT1403792B1 (it) * 2010-12-30 2013-10-31 St Microelectronics Srl Analizzatore per analisi biochimiche e metodo per la determinazione di concentrazioni di sostanze fluorescenti in una soluzione
US9361427B2 (en) * 2011-02-01 2016-06-07 The Regents Of The University Of California Scar-less multi-part DNA assembly design automation
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
EP3081301B1 (de) 2011-05-04 2019-08-14 Luminex Corporation Vorrichtung und verfahren für integrierte probenzubereitung, - reaktion und -erkennung
EP2707509A4 (de) * 2011-05-12 2014-11-26 Netbio Inc Verfahren und zusammensetzungen zur schnellen multiplex-amplifikation von str-loci
EP2525211B1 (de) 2011-05-16 2018-01-03 F. Hoffmann-La Roche AG Instrument und verfahren zum detektieren von analyten
JP5817369B2 (ja) * 2011-09-13 2015-11-18 ソニー株式会社 スペクトル解析装置及び微小粒子測定装置、並びにスペクトル解析あるいはスペクトルチャート表示のための方法及びプログラム
ES2730711T3 (es) 2011-11-03 2019-11-12 Qiagen Gaithersburg Inc Materiales y métodos para inmovilizar, aislar y concentrar células usando superficies carboxiladas
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
US9932632B2 (en) 2012-08-10 2018-04-03 Streck, Inc. Real-time optical system for polymerase chain reaction
EP2929308A4 (de) 2012-12-05 2015-12-16 Genepoc Inc Optische abfragevorrichtung
EP3495803A1 (de) 2013-06-28 2019-06-12 Streck, Inc. Vorrichtungen für echtzeit-polymerasekettenreaktionen
SG10201803344PA (en) 2013-10-14 2018-06-28 Agency Science Tech & Res Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
US9170198B2 (en) * 2013-12-19 2015-10-27 Luminex Corporation Crosstalk reduction
WO2015144783A1 (en) * 2014-03-28 2015-10-01 Curiosity Diagnostics Sp. Z O.O. Device for simultaneous and uniform thermal cycling of samples and uses thereof
BE1022048B1 (nl) * 2014-04-11 2016-02-10 Ugentec Bvba Methoden voor fluorescentie data correctie
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
KR102301305B1 (ko) * 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
KR102373741B1 (ko) * 2017-03-07 2022-03-11 일루미나, 인코포레이티드 단일 광원, 2-광학 채널 서열분석
EP4219770A3 (de) 2017-03-24 2023-08-16 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von rsv b in proben
AU2019226572B2 (en) 2018-03-02 2023-08-24 Life Technologies Corporation Novel quencher and reporter dye combinations
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
CN119510370A (zh) 2019-03-14 2025-02-25 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
US12054770B2 (en) 2020-08-26 2024-08-06 Electronics And Telecommunications Research Institute Apparatus and method for PCR diagnosis based on multi-wavelength light source
CN113308354B (zh) * 2021-05-28 2022-02-15 深圳市尚维高科有限公司 一种多色荧光定量pcr仪光路系统及处理方法
EP4419893A4 (de) * 2021-10-20 2025-08-06 Bio Rad Laboratories Inc Fluoreszenz-nachweissystem
CA3229091A1 (en) 2021-10-26 2023-05-04 Caribou Biosciences, Inc. Exonuclease-coupled real-time endonuclease activity assay
US12320747B2 (en) 2021-11-08 2025-06-03 Electronics And Telecommunications Research Institute Apparatus and method for PCR diagnosis based on multi-wavelength light source and orthogonal code signals
US12385836B2 (en) 2022-08-17 2025-08-12 Electronics And Telecommunications Research Institute Apparatus for PCR diagnosis for normalizing light source power and fluorescence power and operating method thereof
WO2024204236A1 (ja) * 2023-03-27 2024-10-03 株式会社カネカ 核酸増幅用反応液
WO2026050087A1 (en) 2024-08-27 2026-03-05 Caribou Biosciences, Inc. Real-time crispr endonuclease activity assay

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US7081226B1 (en) * 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US5750409A (en) 1991-11-18 1998-05-12 Boehringer Mannheim Gmbh Pentacyclic compounds and their use as absorption or fluorescent dyes
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5869255A (en) * 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
EP0706649B1 (de) 1994-04-29 2001-01-03 Perkin-Elmer Corporation Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
US5563588A (en) * 1994-08-02 1996-10-08 Belfer; Bruce D. Fiber optic traffic signal light system having a shutter control
EP0830586A1 (de) * 1995-05-26 1998-03-25 Optical Analytic Inc. Detektor für weitwinkelstreuung
DE19521231A1 (de) 1995-06-10 1996-12-12 Boehringer Mannheim Gmbh Neue Oxazinfarbstoffe und ihre Verwendung als Fluoreszenzmarker
US5837196A (en) * 1996-01-26 1998-11-17 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
WO1997046707A2 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation System and method for monitoring for dna amplification by fluorescence
US5853992A (en) * 1996-10-04 1998-12-29 The Regents Of The University Of California Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels
US6027709A (en) 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
MA24525A1 (fr) 1997-04-14 1998-12-31 Procter & Gamble Particule detergente
AUPO652997A0 (en) 1997-04-30 1997-05-29 Kindconi Pty Limited Temperature cycling device and method
US6369893B1 (en) 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
ES2183563T3 (es) 1998-05-19 2003-03-16 Cepheid Sistema de deteccion optico multicanal.
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
EP1307592B1 (de) 2000-08-11 2006-07-05 University of Utah Research Foundation Einfach markierte oligonukleotidsonden
US6870165B2 (en) * 2001-10-19 2005-03-22 Biocal Technology, Inc. Multi-color multiplexed analysis in a bio-separation system
US7635588B2 (en) * 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
ATE474933T1 (de) * 2002-12-06 2010-08-15 Hoffmann La Roche Verfahren zum nachweis von pathogenen organismen
WO2004087950A2 (en) 2003-04-04 2004-10-14 Roche Diagnostics Gmbh Improved system for multi color real time pcr

Also Published As

Publication number Publication date
EP2284278A1 (de) 2011-02-16
CA2525482A1 (en) 2004-10-14
WO2004087950A2 (en) 2004-10-14
WO2004087950A3 (en) 2004-11-25
JP2006521092A (ja) 2006-09-21
US20110151550A1 (en) 2011-06-23
CA2525482C (en) 2015-02-17
EP1613771A2 (de) 2006-01-11
JP2009232871A (ja) 2009-10-15
US8137616B2 (en) 2012-03-20
US20060269922A1 (en) 2006-11-30
JP5144605B2 (ja) 2013-02-13
ES2384170T3 (es) 2012-07-02
EP1613771B1 (de) 2012-03-21

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