ATE550348T1 - Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien - Google Patents
Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterienInfo
- Publication number
- ATE550348T1 ATE550348T1 AT08780652T AT08780652T ATE550348T1 AT E550348 T1 ATE550348 T1 AT E550348T1 AT 08780652 T AT08780652 T AT 08780652T AT 08780652 T AT08780652 T AT 08780652T AT E550348 T1 ATE550348 T1 AT E550348T1
- Authority
- AT
- Austria
- Prior art keywords
- polypeptides
- expression
- bacteria
- modified
- increase
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 241000894006 Bacteria Species 0.000 title 1
- 230000029142 excretion Effects 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92887507P | 2007-05-10 | 2007-05-10 | |
| PCT/US2008/063418 WO2008141281A1 (en) | 2007-05-10 | 2008-05-12 | A modified secretion system to increase expression of polypeptides in bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE550348T1 true ATE550348T1 (de) | 2012-04-15 |
Family
ID=39791456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT08780652T ATE550348T1 (de) | 2007-05-10 | 2008-05-12 | Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8343735B2 (de) |
| EP (2) | EP2460823B1 (de) |
| CN (1) | CN101679489B (de) |
| AT (1) | ATE550348T1 (de) |
| DK (2) | DK2152732T3 (de) |
| WO (1) | WO2008141281A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2100947A1 (de) | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Waschmittelzusammensetzung für Spülmaschinen |
| AR076312A1 (es) * | 2009-04-24 | 2011-06-01 | Danisco Us Inc | Proteasas con regiones pro modificadas |
| WO2013043643A1 (en) * | 2011-09-21 | 2013-03-28 | St. Jude Children's Research Hospital | Live, attenuated streptococcus pneumoniae strain and vaccine for protection against pneumococcal disease |
| CN103013960A (zh) * | 2012-12-21 | 2013-04-03 | 青岛蔚蓝生物集团有限公司 | 一种碱性蛋白酶及其重组表达工程菌 |
| CN114395577A (zh) * | 2022-01-06 | 2022-04-26 | 上海应用技术大学 | 一种基因工程菌的制备方法、基因工程菌及重组壳聚糖酶 |
| EP4525615A2 (de) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Zusammensetzungen und verfahren zur prävention, behandlung, unterdrückung und/oder beseitigung von phytopathogenen befällen und infektionen |
| CN116162140B (zh) * | 2022-07-19 | 2025-09-09 | 四川大学华西医院 | 一种幽门螺杆菌重组抗原蛋白SecG及其制备方法与应用 |
| WO2025034713A2 (en) | 2023-08-09 | 2025-02-13 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
| CN119193750B (zh) * | 2024-11-28 | 2025-04-18 | 黑龙江福康生物科技股份有限公司 | 一种牛乳酶解蛋白多肽的制备方法及应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4302544A (en) | 1979-10-15 | 1981-11-24 | University Of Rochester | Asporogenous mutant of B. subtilis for use as host component of HV1 system |
| US4450235A (en) | 1982-04-21 | 1984-05-22 | Cpc International Inc. | Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system |
| US5310675A (en) | 1983-06-24 | 1994-05-10 | Genencor, Inc. | Procaryotic carbonyl hydrolases |
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| DE3480411D1 (en) | 1983-07-06 | 1989-12-14 | Gist Brocades Nv | Molecular cloning and expression in industrial microorganism species |
| US5763257A (en) | 1984-05-29 | 1998-06-09 | Genencor International, Inc. | Modified subtilisins having amino acid alterations |
| EP0254735B2 (de) | 1986-01-15 | 1998-06-17 | Amgen Inc. | Herstellung von ph- und wärmestabile subtilisinanaloge |
| US4980288A (en) | 1986-02-12 | 1990-12-25 | Genex Corporation | Subtilisin with increased thermal stability |
| GB8608194D0 (en) | 1986-04-03 | 1986-05-08 | Massey Ferguson Services Nv | Valve control system |
| WO1988006624A2 (en) | 1987-02-27 | 1988-09-07 | Gist-Brocades N.V. | Molecular cloning and expression of genes encoding proteolytic enzymes |
| US4914031A (en) | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
| DK6488D0 (da) | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
| CN1056187C (zh) | 1988-02-11 | 2000-09-06 | 金克克国际有限公司 | 新的蛋白水解酶及其在洗涤剂中的应用 |
| US5665587A (en) | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
| DK97190D0 (da) | 1990-04-19 | 1990-04-19 | Novo Nordisk As | Oxidationsstabile detergentenzymer |
| US5482849A (en) | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
| ES2121014T3 (es) | 1991-05-01 | 1998-11-16 | Novo Nordisk As | Enzimas estabilizadas y composiciones detergentes. |
| DE4411223A1 (de) | 1994-03-31 | 1995-10-05 | Solvay Enzymes Gmbh & Co Kg | Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren |
| US20030157642A1 (en) * | 1997-07-15 | 2003-08-21 | Caldwell Robert M. | Increasing production of proteins in gram-positive microorganisms |
| AR015977A1 (es) | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| DE69837559T3 (de) | 1997-12-24 | 2011-07-21 | Genencor International, Inc., Calif. | Verfahren zur Bestimmung der Waschleistung eines Enzyms. |
| US6835550B1 (en) | 1998-04-15 | 2004-12-28 | Genencor International, Inc. | Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins |
| US6376450B1 (en) | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
| US6509185B1 (en) | 2000-01-07 | 2003-01-21 | Genencor International, Inc. | Mutant aprE promotor |
| EP1487853A4 (de) | 2002-02-08 | 2006-06-28 | Genencor Int | Sekretions-, transkriptions- und sporulationsgene in bacillus clausii |
-
2008
- 2008-05-12 DK DK08780652.7T patent/DK2152732T3/da active
- 2008-05-12 AT AT08780652T patent/ATE550348T1/de active
- 2008-05-12 WO PCT/US2008/063418 patent/WO2008141281A1/en not_active Ceased
- 2008-05-12 DK DK12157805.8T patent/DK2460823T3/da active
- 2008-05-12 CN CN200880014188.8A patent/CN101679489B/zh not_active Expired - Fee Related
- 2008-05-12 EP EP12157805.8A patent/EP2460823B1/de not_active Not-in-force
- 2008-05-12 EP EP08780652A patent/EP2152732B1/de not_active Not-in-force
- 2008-05-12 US US12/598,182 patent/US8343735B2/en active Active
-
2012
- 2012-10-15 US US13/652,107 patent/US8623630B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20130102052A1 (en) | 2013-04-25 |
| EP2460823B1 (de) | 2014-02-26 |
| US8623630B2 (en) | 2014-01-07 |
| WO2008141281A1 (en) | 2008-11-20 |
| EP2460823A1 (de) | 2012-06-06 |
| DK2152732T3 (da) | 2012-06-04 |
| EP2152732A1 (de) | 2010-02-17 |
| CN101679489A (zh) | 2010-03-24 |
| EP2152732B1 (de) | 2012-03-21 |
| DK2460823T3 (da) | 2014-05-05 |
| US20100184137A1 (en) | 2010-07-22 |
| CN101679489B (zh) | 2014-05-28 |
| US8343735B2 (en) | 2013-01-01 |
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