ATE551420T1 - HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMAL CELLS USING A MULTIPLE TRANSFECTION PROCESS OF MATRIX BINDING AREA SEQUENCES - Google Patents

HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMAL CELLS USING A MULTIPLE TRANSFECTION PROCESS OF MATRIX BINDING AREA SEQUENCES

Info

Publication number
ATE551420T1
ATE551420T1 AT08153753T AT08153753T ATE551420T1 AT E551420 T1 ATE551420 T1 AT E551420T1 AT 08153753 T AT08153753 T AT 08153753T AT 08153753 T AT08153753 T AT 08153753T AT E551420 T1 ATE551420 T1 AT E551420T1
Authority
AT
Austria
Prior art keywords
sequence
expression
high efficiency
gene transfer
binding area
Prior art date
Application number
AT08153753T
Other languages
German (de)
Inventor
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
Duc-Quang Nguyen
David Calabrese
Damien Saugy
Stefania Puttini
Original Assignee
Selexis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Selexis Sa filed Critical Selexis Sa
Application granted granted Critical
Publication of ATE551420T1 publication Critical patent/ATE551420T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A purified and isolated DNA sequence has protein production increasing activity. Said DNA sequence comprises at least one bent DNA element, and at least one binding site for a DNA binding protein. It is a MAR nucleotide with the sequence SEQ lD No 27, a sequence complementary thereof, or a sequence having at least 70% identity with said sequence. Advantageously the bent DNA element contains at least 10% of dinucleotide TA, and/or at least 12% of dinucleotide AT on a stretch of 100 contiguous base pairs.
AT08153753T 2003-10-24 2004-10-22 HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMAL CELLS USING A MULTIPLE TRANSFECTION PROCESS OF MATRIX BINDING AREA SEQUENCES ATE551420T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51357403P 2003-10-24 2003-10-24
EP04002722 2004-02-06

Publications (1)

Publication Number Publication Date
ATE551420T1 true ATE551420T1 (en) 2012-04-15

Family

ID=34524793

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08153753T ATE551420T1 (en) 2003-10-24 2004-10-22 HIGH EFFICIENCY GENE TRANSFER AND EXPRESSION IN MAMMAL CELLS USING A MULTIPLE TRANSFECTION PROCESS OF MATRIX BINDING AREA SEQUENCES

Country Status (15)

Country Link
US (3) US8252917B2 (en)
EP (5) EP1959011B1 (en)
JP (1) JP5396653B2 (en)
KR (4) KR101269656B1 (en)
CN (1) CN1863913B (en)
AT (1) ATE551420T1 (en)
AU (4) AU2004284220B2 (en)
CA (1) CA2535836C (en)
IL (2) IL175142A0 (en)
IN (2) IN2014DN02484A (en)
PL (4) PL2292754T3 (en)
RU (1) RU2375450C2 (en)
SG (1) SG147468A1 (en)
WO (1) WO2005040377A2 (en)
ZA (1) ZA200604032B (en)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1572994T3 (en) 2002-12-20 2007-05-29 Chromagenics Bv Means and Methods for Preparing a Protein Through Chromatin Openers That Can Make Chromatin More Available for Transcription Factors
KR101269656B1 (en) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
DE102004043207C5 (en) * 2004-09-03 2010-09-09 Südzucker AG Mannheim/Ochsenfurt Root and xylem parenchyma specific promoter
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
BRPI0517380A (en) 2004-11-08 2008-10-07 Chromagenics Bv DNA molecule, expression cassette, host cell, and methods for generating a host cell expressing a polypeptide of interest and for producing a polypeptide of interest
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1996705B1 (en) 2006-03-20 2011-08-31 ChromaGenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
JP2010501170A (en) * 2006-08-23 2010-01-21 セレキス エスアー Matrix attachment region (MAR) and its use to increase transcription
EP1975228A1 (en) 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
US8563002B2 (en) 2007-12-24 2013-10-22 Glaxosmithkline Biologicals, S.A. Recombinant RSV antigens
CN101580842B (en) * 2008-05-15 2011-02-09 中国农业科学院生物技术研究所 DNA molecule capable of reinforcing gene expression and use thereof
EP2379598B1 (en) 2009-01-19 2015-03-11 Innate Pharma Anti-kir3d antibodies
SG176807A1 (en) 2009-06-24 2012-01-30 Id Biomedical Corp Quebec Vaccine
PL2445526T3 (en) 2009-06-24 2017-08-31 Glaxosmithkline Biologicals S.A. Recombinant rsv antigens
EP4183412B1 (en) 2009-07-15 2024-08-14 GlaxoSmithKline Biologicals SA Rsv f protein compositions and methods for making same
CN102575264A (en) * 2009-09-18 2012-07-11 瑟莱克斯公司 Products and methods for enhanced transgene expression and processing
WO2011070405A1 (en) * 2009-12-07 2011-06-16 Avesthagen Limited An expression vector carrying scaffold/matrix attachment region(s)
RU2422512C1 (en) * 2010-02-16 2011-06-27 Автономная некоммерческая организация "Институт молекулярной диагностики" (АНО "ИнМоДи") Escherichia coli BL21(DE3)/pAFP11D3 STRAIN - PRODUCER OF 404 TO 609 AMINO ACID FRAGMENT OF HUMAN ALPHA-FETOPROTEIN
WO2012019630A1 (en) 2010-08-13 2012-02-16 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein
WO2012095514A1 (en) 2011-01-14 2012-07-19 Vivalis Recombinant protein production system
EP2697375B1 (en) 2011-04-13 2017-10-18 National Research Council of Canada Expression system with scaffold attachment region (sar) element from interferon 2
RS56748B1 (en) 2011-05-13 2018-03-30 Glaxosmithkline Biologicals Sa Pre-fusion rsv f antigens
WO2013120497A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein
WO2013120500A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen
WO2013120498A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded allergenic antigen or an autoimmune self-antigen
WO2013120499A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen
RU2658490C2 (en) 2012-03-27 2018-06-21 Кьюрвак Аг Artificial nucleic acid molecules for improved protein or peptide expression
DK2951309T3 (en) 2013-02-01 2019-04-23 Selexis Sa INCREASED TRANSGEN EXPRESSION AND PROCESSING
CN103667440A (en) * 2013-09-05 2014-03-26 谢小冬 Application of SNP loci of GATA4 genes
US9982027B2 (en) 2013-10-22 2018-05-29 Lubris Llc Control of rheological properties of mixed hyaluronate/lubricin solutions
CN109576269B (en) * 2014-04-01 2020-08-28 三生国健药业（上海）股份有限公司 Animal cell expression vector containing MAR core fragment
CN104974933B (en) * 2014-04-04 2017-08-15 上海泰因生物技术有限公司 A kind of extensive continuous several times, which suspend, turns the apparatus and method of expression recombinant protein wink
US11518791B2 (en) 2016-05-23 2022-12-06 Luxembourg Institute Of Health (Lih) Multifunctional heteromultimeric constructs
EP3456821B2 (en) * 2017-09-19 2024-01-24 Deutsches Krebsforschungszentrum Non-integrating dna vectors for the genetic modification of cells
US20230193341A1 (en) 2018-10-24 2023-06-22 Selexis Sa Expression systems, recombinant cells and uses thereof
WO2020118169A1 (en) 2018-12-07 2020-06-11 Celltheon Corporation Matrix attachment regions and uses in promoting gene expression
KR20220145361A (en) 2020-02-24 2022-10-28 노파르티스 아게 Purification of Recombinantly Produced Polypeptides
US20230192893A1 (en) * 2020-05-11 2023-06-22 Samsung Bioepis Co., Ltd. Human genome-derived polynucleotide and method for producing polypeptide of interest using same
US20230293690A1 (en) * 2020-07-22 2023-09-21 Deutsches Krebsforschungszentrum 5' s/mar applications
CN121137185B (en) * 2025-11-20 2026-03-03 中国科学院海洋研究所 A molecular marker, primer pair and its application related to mussel species

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1077994A (en) * 1963-04-18 1967-08-02 Kobe Steel Ltd Process for producing cold-forged products from tempered steel wire
DE2605560C3 (en) 1976-02-12 1979-08-30 Siemens Ag, 1000 Berlin Und 8000 Muenchen Digestion process for biomaterials
EP0113551B1 (en) 1982-12-23 1988-04-27 Britax Weathershields Limited Vehicle sliding roof
DE3751873T2 (en) 1986-04-09 1997-02-13 Genzyme Corp Genetically transformed animals that secrete a desired protein in milk
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
GB8925590D0 (en) 1989-11-13 1990-01-04 Central Blood Lab Authority Monoclonal antibodies
US5968502A (en) 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
ES2176208T3 (en) 1992-10-05 2002-12-01 Univ North Carolina State METHOD TO INCREASE EXPRESSION AND TO REDUCE THE VARIABILITY OF EXPRESSION OF FORANE GENES IN VEGETABLE CELLS.
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5888981A (en) 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5907078A (en) 1994-12-09 1999-05-25 Greenberg; Norman M. Transgenic mouse model for prostate cancer
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
DE19541450C2 (en) 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Gene construct and its use
US6596514B2 (en) 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
AU2064297A (en) 1996-02-29 1997-09-16 Immusol, Inc Hepatitis c virus ribozymes
CA2256484A1 (en) 1996-06-06 1997-12-11 Novartis Ag Vectors comprising sar elements
CA2689842A1 (en) 1996-12-02 1998-06-11 Genaera Corporation Il-9 receptor variants, useful in treating and diagnosing atopic allergies including asthma and related disorders
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6252058B1 (en) 1997-11-05 2001-06-26 Timothy C. Thompson Sequences for targeting metastatic cells
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6004284A (en) 1998-06-04 1999-12-21 Alcon Laboratories, Inc. Surgical handpiece
WO2000005393A2 (en) 1998-07-21 2000-02-03 Cobra Therapeutics Limited A polynucleotide comprising a ubiquitous chromatin opening element (ucoe)
US6338066B1 (en) 1998-09-25 2002-01-08 International Business Machines Corporation Surfaid predictor: web-based system for predicting surfer behavior
AU1105600A (en) 1998-10-07 2000-04-26 Adobe Systems Incorporated Distributing access to a data item
DE19848017A1 (en) 1998-10-17 2000-04-20 Multigene Biotech Gmbh Episomally replicating vectors, useful in gene therapy, contain origins of replication and scaffold-matrix attached region sequences but no viral coding sequences
ATE279524T1 (en) * 1998-12-01 2004-10-15 Dow Agrosciences Llc ARTIFICIAL MATRIX ATTACHMENT REGION TO INCREASE EXPRESSION OF GENES INTRODUCED INTO PLANTS
US6225113B1 (en) 1998-12-04 2001-05-01 Genvec, Inc. Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
AU4940700A (en) 1999-05-28 2000-12-18 Sangamo Biosciences, Inc. Gene switches
US20050129669A1 (en) 1999-06-21 2005-06-16 Transkaryotic Therapies, Inc., A Massachusetts Corporation DNA construct for effecting homologous recombination and uses thereof
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
JP3507858B2 (en) 1999-11-19 2004-03-15 奈良先端科学技術大学院大学長 DNA fragments that stabilize the expression of foreign genes in plants, gene introduction methods for stably expressing foreign genes in plants, transformed plants that stably express foreign genes
WO2001040488A1 (en) 1999-11-30 2001-06-07 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
US6864085B2 (en) 1999-12-14 2005-03-08 Novartis Ag Bovine immunodeficiency virus (BIV) based vectors
US20020127199A1 (en) 2000-02-03 2002-09-12 Tang Y. Tom Novel nucleic acids and polypeptides
US6821775B1 (en) 2000-02-11 2004-11-23 Genvec, Inc. Viral vector encoding pigment epithelium-derived factor
DE10006886A1 (en) 2000-02-16 2001-08-23 Hepavec Ag Fuer Gentherapie Non-human helper dependent virus vector
US6569681B1 (en) 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US6747189B1 (en) 2000-03-21 2004-06-08 Dekalb Genetics Corporation Maize glycine rich protein promoter compositions and methods for use thereof
US6730826B2 (en) 2000-03-22 2004-05-04 University Of Kentucky Research Foundation Plant trichome gland specific promoter sequence
US20030157715A1 (en) 2000-06-26 2003-08-21 Ulrich Laemmli Modulation of chromosome function by chromatin remodeling agents
EP1294406A2 (en) 2000-06-26 2003-03-26 Universite De Geneve Modulation of chromosome function by chromatin remodeling agents
US8030537B1 (en) 2000-07-27 2011-10-04 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for the production of recombinant proteins, cells and organs
KR100408844B1 (en) 2000-07-29 2003-12-06 한국산업기술평가원 Expression vector using for animal cell
WO2002018607A2 (en) 2000-08-30 2002-03-07 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
AU9379801A (en) 2000-09-09 2002-03-22 Basf Plant Science Gmbh Modified tet-inducible system for regulation of gene expression in plants
GB0022995D0 (en) 2000-09-20 2000-11-01 Cobra Therapeutics Ltd Polynucleotide
US6919204B2 (en) 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
WO2002046208A2 (en) 2000-11-01 2002-06-13 Elusys Therapeutics, Inc. Method of producing biospecific molecules by protein trans-splicing
DE10061150A1 (en) 2000-12-08 2002-06-13 Icon Genetics Ag Methods and vectors for the production of transgenic plants
US7259010B2 (en) 2000-12-15 2007-08-21 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region of interferon beta
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
DK1395669T3 (en) 2001-01-26 2009-11-16 Selexis Sa Matrix binding regions and methods for using them
DE10109780A1 (en) 2001-02-28 2002-09-05 Cardion Ag Episomal vectors containing matrix attachment regions for cell-specific gene expression
WO2002072138A1 (en) 2001-03-08 2002-09-19 Hyseq, Inc. Methods and materials relating to fibulin-like polypeptides and polynucleotides
JP4817514B2 (en) 2001-03-09 2011-11-16 協和発酵キリン株式会社 Novel animal cell vectors and uses thereof
WO2002072789A2 (en) 2001-03-12 2002-09-19 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
EP1967590A3 (en) 2001-03-13 2008-09-17 Novartis AG Lentiviral packaging constructs
WO2002079447A2 (en) 2001-03-30 2002-10-10 Avigenics, Inc. Avian lysozyme promoter
DE10129010A1 (en) 2001-06-13 2002-12-19 Icon Genetics Ag Producing a transgenic plant or plant cell expressing a transgene under control of native transcription and translation elements already present in the genome is useful to give stable expression of transgenes
US20030032597A1 (en) 2001-07-31 2003-02-13 Sebestyen Magdolna G. Targeting nucleic acids to a cellular nucleus
KR100464962B1 (en) * 2001-09-14 2005-01-05 삼화강봉주식회사 Quenched & tempered steel wire with superior characteristics of cold forging
US7122715B2 (en) 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
AU2002341795A1 (en) 2001-09-21 2003-04-01 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US7138278B2 (en) 2001-11-20 2006-11-21 Monsanto Technology, L.L.C. Maize cytoplasmic glutamine synthetase promoter compositions and methods for use thereof
FR2832423B1 (en) 2001-11-22 2004-10-08 Vivalis EXOGENOUS PROTEIN EXPRESSION SYSTEM IN AN AVIAN SYSTEM
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
CA2427190A1 (en) 2002-04-30 2003-10-30 Alberta Research Council Inc. Production of recombinant epidermal growth factor in plants
US20030224477A1 (en) 2002-05-31 2003-12-04 Heartlein Michael W. Optimized promoter constructs
EP1513929A4 (en) 2002-06-18 2006-04-19 Zymogenetics Inc Hybrid vector having a cytomegalovirus enhancer and myeloproliferative sarcoma virus promoter
US20040016015A1 (en) 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US6933136B2 (en) 2002-09-20 2005-08-23 Novo Nordisk A/S Method for making recombinant proteins
CN1263860C (en) 2002-09-30 2006-07-12 华南农业大学 Construction method of multigene carrier and its application
WO2004053106A2 (en) 2002-12-05 2004-06-24 Regulome Corporation Profiled regulatory sites useful for gene control
WO2004053137A2 (en) 2002-12-11 2004-06-24 Cytos Biotechnology Ag Method for protein production
AU2002953381A0 (en) 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1445320A1 (en) 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automated gene-targeting using non-toxic detectable markers
WO2004094640A1 (en) 2003-04-15 2004-11-04 University Of Georgia Research Foundation, Inc. Avian transgenesis using a chicken ovalbumin gene region
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
EP1644508A1 (en) 2003-07-11 2006-04-12 Cytos Biotechnology AG Gene expression system
JP2007503212A (en) 2003-08-27 2007-02-22 オーアールエフ・リフタエクニ・エイチエフ Methods for enhancing the accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins
CA2541523A1 (en) 2003-10-21 2005-05-06 Applied Research Systems Ars Holding N.V. Minimal dna sequence acting as a chromatin insulator and its use in protein expression
KR101269656B1 (en) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
JP2010501170A (en) 2006-08-23 2010-01-21 セレキス エスアー Matrix attachment region (MAR) and its use to increase transcription
EP2097538A4 (en) * 2006-12-07 2011-11-30 Switchgear Genomics TRANSCRIPTIONAL REGULATION ELEMENTS OF BIOLOGICAL PATHWAYS, TOOLS, AND METHODS

Also Published As

Publication number Publication date
US10669562B2 (en) 2020-06-02
RU2006117757A (en) 2007-11-27
CA2535836C (en) 2014-02-04
US20180187229A1 (en) 2018-07-05
SG147468A1 (en) 2008-11-28
KR101269656B1 (en) 2013-05-30
EP2292754B1 (en) 2012-12-26
AU2010236091A1 (en) 2010-11-25
EP1675952A2 (en) 2006-07-05
KR20110112884A (en) 2011-10-13
IL198863A (en) 2011-09-27
EP2292754A1 (en) 2011-03-09
RU2375450C2 (en) 2009-12-10
IN2014DN02483A (en) 2015-05-15
WO2005040377A3 (en) 2005-09-15
HK1120549A1 (en) 2009-04-03
JP5396653B2 (en) 2014-01-22
IN2014DN02484A (en) 2015-05-15
EP2298898B1 (en) 2012-12-26
EP1959011A2 (en) 2008-08-20
CA2535836A1 (en) 2005-05-06
PL2287302T3 (en) 2013-05-31
US20070178469A1 (en) 2007-08-02
AU2010236072A1 (en) 2010-11-18
US9879297B2 (en) 2018-01-30
KR20110112883A (en) 2011-10-13
AU2010236093B2 (en) 2011-10-13
AU2010236093A1 (en) 2010-12-02
CN1863913A (en) 2006-11-15
AU2010236072B2 (en) 2011-09-01
HK1151825A1 (en) 2012-02-10
KR101230838B1 (en) 2013-02-06
US8252917B2 (en) 2012-08-28
HK1149777A1 (en) 2011-10-14
PL1959011T3 (en) 2012-07-31
PL2298898T3 (en) 2013-05-31
EP2298898A1 (en) 2011-03-23
PL2292754T3 (en) 2013-06-28
KR101222025B1 (en) 2013-01-15
EP2287302A1 (en) 2011-02-23
EP2287302B1 (en) 2012-12-26
KR20110112882A (en) 2011-10-13
JP2007508831A (en) 2007-04-12
ZA200604032B (en) 2008-05-28
AU2010236091B2 (en) 2011-07-07
EP1959011B1 (en) 2012-03-28
AU2004284220A1 (en) 2005-05-06
WO2005040377A2 (en) 2005-05-06
US20130143264A1 (en) 2013-06-06
IL175142A0 (en) 2006-09-05
EP1959011A3 (en) 2008-08-27
HK1151824A1 (en) 2012-02-10
AU2004284220B2 (en) 2010-10-14
KR20060100372A (en) 2006-09-20
CN1863913B (en) 2011-01-12
KR101229989B1 (en) 2013-02-06

Similar Documents

Publication Publication Date Title
PL2292754T3 (en) High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
ATE471370T1 (en) VARIANTS OF BETA-GLUCOSIDASES
ES2375058T8 (en) AXMI-010, A GENE OF DELTA-ENDOTOXIN AND METHODS FOR USE.
AR057205A1 (en) GRG23 AND GRG51 GENES THAT CONFERENCE RESISTANCE TO HERBICIDES
ATE414779T1 (en) VECTORS FOR TRANSFORMING PLANTS
CY1108240T1 (en) Isolated Polynucleotides Having a Reduced 5΄CpG3΄ Motifs Content and Their Uses
CY1106041T1 (en) BRAND NEW COLLECTOR
CY1106978T1 (en) PHOSPHOSESTERATION ENZYME
ES2338285T3 (en) NEW SPECIFIC GENES OF THE BASE OF BASE TRANSFER OF ENDOSPERM CELLS (BETL).
ATE505549T1 (en) MALE FERTILITY-PROVIDING NUCLEOTIDE SEQUENCES AND THEIR USE
WO2003046123A3 (en) Mutated gene from corynebacterium glutamicum
ATE465259T1 (en) NUCLEOTIDE SEQUENCES ENCODING THE DAPC GENE AND METHOD FOR PRODUCING L-LYSINE
TR200003272T2 (en) Genomic sequences of the G-CSF gene for protein production delivery.
DE50212612D1 (en) GENES THAT CODE FOR CARBON METABOLISM AND ENERGY PRODUCTION PROTEINS
BR9902248A (en) DNA fragment, and, dna.
AR041953A1 (en) DNA SEQUENCES FROM THE TOT PHOTORHABDUS LUMINISCENS GENOMIC REGION
ATE346153T1 (en) ENDOGENEOUS RETROVIRAL SEQUENCES, RELATED TO AUTO-IMMUNE DISEASES OR TO PREGNANCY DISORDERS
DE602004029180D1 (en) NEW PLASMIDE AND ITS USE
Bailey et al. Molecular origin of the mosaic sequence arrangements of higher primate α-globin duplication units
NO20021255D0 (en) New cell cycle checkpoint genes and proteins encoded thereof
ATE339508T1 (en) MITES-LIKE ELEMENT AND ELEMENT INVOLVED IN TRANSCRIPTION ACTIVATION
TR200003271T2 (en) Genomic sequences for protein production and delivery.
ATE485380T1 (en) NUCLEIC ACIDS AND PROTEINS EXHIBIT INCREASED EXPRESSION UNDER SALT STRESS
TH19539A (en) New genes derived from corine to form bacteria and using them
MXPA00001973A (en) Sequence-determined dna fragments and corresponding polypeptides encoded therapy.