ATE552339T1 - Signalverstärkungsverfahren zum nachweis eines mutanten gens - Google Patents
Signalverstärkungsverfahren zum nachweis eines mutanten gensInfo
- Publication number
- ATE552339T1 ATE552339T1 AT04713241T AT04713241T ATE552339T1 AT E552339 T1 ATE552339 T1 AT E552339T1 AT 04713241 T AT04713241 T AT 04713241T AT 04713241 T AT04713241 T AT 04713241T AT E552339 T1 ATE552339 T1 AT E552339T1
- Authority
- AT
- Austria
- Prior art keywords
- signal amplification
- detecting
- amplification method
- mutant gene
- detection sensitivity
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 abstract 3
- 238000000018 DNA microarray Methods 0.000 abstract 2
- 238000001338 self-assembly Methods 0.000 abstract 2
- 230000035945 sensitivity Effects 0.000 abstract 2
- 102000012410 DNA Ligases Human genes 0.000 abstract 1
- 108010061982 DNA Ligases Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003044859 | 2003-02-21 | ||
| PCT/JP2004/002007 WO2004074480A1 (ja) | 2003-02-21 | 2004-02-20 | 変異遺伝子検出のためのシグナル増幅方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE552339T1 true ATE552339T1 (de) | 2012-04-15 |
Family
ID=32905470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04713241T ATE552339T1 (de) | 2003-02-21 | 2004-02-20 | Signalverstärkungsverfahren zum nachweis eines mutanten gens |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7632639B2 (de) |
| EP (1) | EP1595953B1 (de) |
| JP (1) | JP4255472B2 (de) |
| KR (1) | KR101111568B1 (de) |
| CN (1) | CN100532550C (de) |
| AT (1) | ATE552339T1 (de) |
| AU (1) | AU2004213716B2 (de) |
| CA (1) | CA2516360C (de) |
| WO (1) | WO2004074480A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE526402T1 (de) | 2005-02-28 | 2011-10-15 | Eisai R&D Man Co Ltd | Hilfssonden und verfahren zur verwendung davon |
| JP4616881B2 (ja) * | 2005-02-28 | 2011-01-19 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | アシストプローブ及びその利用方法 |
| EP1939622B1 (de) * | 2005-09-27 | 2011-09-21 | Eisai R&D Management Co., Ltd. | Verfahren zur bildung von autoaggregat auf einem mikropartikel und verfahren zum nachweis von zielanalyten |
| EP1997908A4 (de) | 2006-03-15 | 2010-09-29 | Eisai R&D Man Co Ltd | Verfahren zum bilden eines signalsondenpolymers |
| EP2180063B1 (de) * | 2007-08-14 | 2013-01-09 | Eisai R&D Management Co., Ltd. | Verfahren zur erkennung einer zielsubstanz |
| WO2009022683A1 (ja) * | 2007-08-14 | 2009-02-19 | Eisai R & D Management Co., Ltd. | 標的物質の検出方法 |
| EE201000013A (et) | 2010-01-29 | 2011-10-17 | Selfdiagnostics O� | Meetod ja kiirtesti seade sihtmrk-molekuli proovimaterjalist detekteerimiseks |
| CN102304565B (zh) * | 2011-04-29 | 2014-03-05 | 益善生物技术股份有限公司 | 一种hfe基因多态性检测特异性引物和液相芯片 |
| WO2013085026A1 (ja) | 2011-12-09 | 2013-06-13 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 塩基変異の検出方法及び検出用キット |
| ES2759337T3 (es) * | 2014-10-14 | 2020-05-08 | Abbott Lab | ADN de conversión de secuencia y ADN amplificador de señal que tiene secuencias espaciadoras de poli-ADN y métodos de detección que los utilizan |
| CN108277260A (zh) * | 2018-04-16 | 2018-07-13 | 吉林大学 | 一种检测brafv600e基因突变的方法 |
| JP7161173B2 (ja) * | 2018-06-29 | 2022-10-26 | 積水メディカル株式会社 | ｓｉＲＮＡの定量方法 |
| US11993806B2 (en) * | 2018-09-11 | 2024-05-28 | Sekisui Medical Co., Ltd. | Method of detecting or method of quantifying oligonucleotides |
| WO2021095829A1 (ja) * | 2019-11-14 | 2021-05-20 | 積水メディカル株式会社 | オリゴヌクレオチドの検出又は定量方法 |
| KR20210109745A (ko) * | 2020-02-28 | 2021-09-07 | 주식회사 누리바이오 | 단일 표적 유전자의 유전적 변이 실시간 검출용 단일핵산 및 이를 이용한 검출 방법 |
| CN115992227B (zh) * | 2021-10-20 | 2025-09-19 | 益善生物技术股份有限公司 | 一种Hepatocyte检测试剂盒及其制备方法与应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| DK51092D0 (da) | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| WO1995021271A1 (en) | 1994-02-07 | 1995-08-10 | Molecular Tool, Inc. | Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis |
| US5985557A (en) | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
| US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US20020150921A1 (en) | 1996-02-09 | 2002-10-17 | Francis Barany | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| EP2332957B1 (de) | 1996-02-09 | 2015-04-08 | Cornell Research Foundation, Inc. | Detektion von Unterschieden in der Sequenz von Nukleinsäuren mittels der Ligasedetektionsreaktion und adressierbaren Arrays |
| EP1112378A1 (de) * | 1998-07-17 | 2001-07-04 | GeneTag Technology, Inc. | Methoden zur detektion und kartierung von genen, mutationen und abweichender polynukleotidsequenzen |
| US6211111B1 (en) | 1998-08-17 | 2001-04-03 | The Dow Chemical Company | Activator composition comprising aluminum compound mixture |
| JP3267576B2 (ja) | 1998-11-09 | 2002-03-18 | 三光純薬株式会社 | プローブ高分子の形成方法 |
| KR100446358B1 (ko) | 2000-03-31 | 2004-09-01 | 산코 준야쿠 가부시키가이샤 | 프로우브 중합체 제작용 프로우브, 프로우브 중합체의 제작방법 및 그 이용 |
| KR20020065471A (ko) * | 2000-08-30 | 2002-08-13 | 산코 준야쿠 가부시키가이샤 | 유전자의 검출방법 |
| US20050130139A1 (en) * | 2001-09-28 | 2005-06-16 | Mitsugu Usui | Method f amplifying dna chip signals |
| JPWO2003040367A1 (ja) * | 2001-11-08 | 2005-03-03 | 三光純薬株式会社 | 遺伝子増幅反応で合成されたオリゴヌクレオチドによる自己集合体の形成方法、自己集合体及び遺伝子の検出方法 |
-
2004
- 2004-02-20 AU AU2004213716A patent/AU2004213716B2/en not_active Ceased
- 2004-02-20 WO PCT/JP2004/002007 patent/WO2004074480A1/ja not_active Ceased
- 2004-02-20 EP EP04713241A patent/EP1595953B1/de not_active Expired - Lifetime
- 2004-02-20 JP JP2005502795A patent/JP4255472B2/ja not_active Expired - Fee Related
- 2004-02-20 US US10/546,593 patent/US7632639B2/en not_active Expired - Fee Related
- 2004-02-20 AT AT04713241T patent/ATE552339T1/de active
- 2004-02-20 CN CNB2004800038183A patent/CN100532550C/zh not_active Expired - Fee Related
- 2004-02-20 KR KR1020057013000A patent/KR101111568B1/ko not_active Expired - Fee Related
- 2004-02-20 CA CA2516360A patent/CA2516360C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2516360C (en) | 2012-07-10 |
| JP4255472B2 (ja) | 2009-04-15 |
| EP1595953A4 (de) | 2007-02-28 |
| CN1748028A (zh) | 2006-03-15 |
| AU2004213716B2 (en) | 2008-02-28 |
| EP1595953A1 (de) | 2005-11-16 |
| KR101111568B1 (ko) | 2012-03-05 |
| EP1595953B1 (de) | 2012-04-04 |
| US7632639B2 (en) | 2009-12-15 |
| US20060210983A1 (en) | 2006-09-21 |
| WO2004074480A1 (ja) | 2004-09-02 |
| CA2516360A1 (en) | 2004-09-02 |
| CN100532550C (zh) | 2009-08-26 |
| AU2004213716A1 (en) | 2004-09-02 |
| JPWO2004074480A1 (ja) | 2006-06-01 |
| KR20050106398A (ko) | 2005-11-09 |
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