ATE554185T1 - Verfahren und konstrukte zur bewertung von rnai- zielen und effektormolekülen - Google Patents

Verfahren und konstrukte zur bewertung von rnai- zielen und effektormolekülen

Info

Publication number
ATE554185T1
ATE554185T1 AT04714072T AT04714072T ATE554185T1 AT E554185 T1 ATE554185 T1 AT E554185T1 AT 04714072 T AT04714072 T AT 04714072T AT 04714072 T AT04714072 T AT 04714072T AT E554185 T1 ATE554185 T1 AT E554185T1
Authority
AT
Austria
Prior art keywords
methods
constructs
evaluation
effector molecules
rnai
Prior art date
Application number
AT04714072T
Other languages
English (en)
Inventor
Catherine Pachuk
Original Assignee
Alnylam Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alnylam Pharmaceuticals Inc filed Critical Alnylam Pharmaceuticals Inc
Application granted granted Critical
Publication of ATE554185T1 publication Critical patent/ATE554185T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT04714072T 2003-02-27 2004-02-24 Verfahren und konstrukte zur bewertung von rnai- zielen und effektormolekülen ATE554185T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45107003P 2003-02-27 2003-02-27
PCT/US2004/005065 WO2004076629A2 (en) 2003-02-27 2004-02-24 METHODS AND CONSTRUCTS FOR EVALUATION OF RNAi TARGETS AND EFFECTOR MOLECULES

Publications (1)

Publication Number Publication Date
ATE554185T1 true ATE554185T1 (de) 2012-05-15

Family

ID=32927699

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04714072T ATE554185T1 (de) 2003-02-27 2004-02-24 Verfahren und konstrukte zur bewertung von rnai- zielen und effektormolekülen

Country Status (6)

Country Link
US (1) US20060263764A1 (de)
EP (1) EP1597351B1 (de)
AT (1) ATE554185T1 (de)
AU (1) AU2004214954A1 (de)
CA (1) CA2517235A1 (de)
WO (1) WO2004076629A2 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005021752A1 (ja) * 2003-08-29 2005-03-10 Takara Bio Inc. 機能的ヌクレオチド分子の検索方法
WO2005038054A1 (en) * 2003-10-20 2005-04-28 Zicai Liang METHOD OF MEASURING THE EFFICACY OF siRNA MOLECULES
AU2005222965B8 (en) 2004-03-15 2010-07-01 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
US20070265220A1 (en) 2004-03-15 2007-11-15 City Of Hope Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
EP2169072A1 (de) 2004-08-23 2010-03-31 Alnylam Pharmaceuticals, Inc Expressionskonstrukte mit mehreren RNA-Polymerase-III-Promotoren
ES2362670T3 (es) 2004-09-24 2011-07-11 Alnylam Pharmaceuticals, Inc Orientación a intermedios de replicación de hebra no codificante de virus monocatenarios por arni.
CA2592099A1 (en) 2004-12-22 2006-06-29 Nucleonics, Inc. Conserved hbv and hcv sequences useful for gene silencing
EP1969137B1 (de) * 2005-11-22 2011-10-05 Stichting Dienst Landbouwkundig Onderzoek Multiplex-nukleinsäurenachweis
EP2548438B1 (de) 2006-11-08 2015-08-05 Veritas Bio, LLC In-vivo-Abgabe von Doppelstrang-RNA an eine Zielzelle
WO2008112127A2 (en) * 2007-03-08 2008-09-18 Switchgear Genomics Functional arrays for high throughput characterization of regulatory elements in untranslated regions of genes
KR101142209B1 (ko) 2007-09-22 2012-05-04 재단법인서울대학교산학협력재단 섭취 ＲＮＡｉ를 이용한 꼬마선충에서 두 유전자의동시발현 억제방법
CN101173294B (zh) * 2007-10-12 2011-01-26 中山大学 一种基因微链载体及其构建方法和应用
US8815779B2 (en) 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
KR102055331B1 (ko) 2011-04-21 2019-12-12 아이오니스 파마수티컬즈, 인코포레이티드 B형 간염 바이러스(hbv) 발현 조절
US10307226B2 (en) 2013-03-14 2019-06-04 National Dentex, Llc Bone foundation guide and method of use
US10278789B2 (en) 2013-03-14 2019-05-07 National Dentex, Llc Bone foundation guide system and method
US10398530B2 (en) 2013-03-14 2019-09-03 National Dentex, Llc Bone foundation guide system and method
HK1247622A1 (zh) 2015-03-02 2018-09-28 180 Therapeutics Lp 利用il-33拮抗剂来治疗局部纤维化的方法
JP2018531140A (ja) 2015-10-23 2018-10-25 リョップ・ダニエル・アールＬＬＯＰ， Ｄａｎｉｅｌ， Ｒ． 骨土台ガイドシステムおよび方法
CA3015801A1 (en) 2016-03-05 2017-09-14 National Dentex, Llc Bone foundation guide system and method
EP3548005A4 (de) 2016-11-29 2020-06-17 Puretech Health LLC Exosome zur ausgabe von therapeutischen wirkstoffen
KR102234135B1 (ko) * 2019-10-30 2021-03-31 서울대학교산학협력단 GFP 유전자의 발현을 억제하는 dsRNA 및 이의 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5738985A (en) * 1993-04-02 1998-04-14 Ribogene, Inc. Method for selective inactivation of viral replication
US6013447A (en) 1997-11-21 2000-01-11 Innovir Laboratories, Inc. Random intracellular method for obtaining optimally active nucleic acid molecules
US20020002278A1 (en) * 1999-11-05 2002-01-03 Timothy W. Nilsen Random intracellular method for obtaining optimally active nucleic acid molecules
DE60130583T3 (de) * 2000-12-01 2018-03-22 Europäisches Laboratorium für Molekularbiologie Kleine rns moleküle, die rns-interferenz vermitteln
CA2369944A1 (en) 2001-01-31 2002-07-31 Nucleonics Inc. Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
EP1392847B1 (de) * 2001-05-04 2006-05-31 Health Research, Inc. Test mit hohem durchsatz zur identifizierung von modifikatoren der genexpression

Also Published As

Publication number Publication date
EP1597351A2 (de) 2005-11-23
US20060263764A1 (en) 2006-11-23
WO2004076629A3 (en) 2005-10-27
EP1597351A4 (de) 2006-06-14
WO2004076629A2 (en) 2004-09-10
AU2004214954A1 (en) 2004-09-10
EP1597351B1 (de) 2012-04-18
CA2517235A1 (en) 2004-09-10

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