ATE555196T1 - Wärmebeständige duplexspezifische nuklease - Google Patents

Wärmebeständige duplexspezifische nuklease

Info

Publication number
ATE555196T1
ATE555196T1 AT09169259T AT09169259T ATE555196T1 AT E555196 T1 ATE555196 T1 AT E555196T1 AT 09169259 T AT09169259 T AT 09169259T AT 09169259 T AT09169259 T AT 09169259T AT E555196 T1 ATE555196 T1 AT E555196T1
Authority
AT
Austria
Prior art keywords
specific nuclease
protein
duplex
nuclease activity
dsn
Prior art date
Application number
AT09169259T
Other languages
English (en)
Inventor
Yuzo Hirakawa
Isao Ohiso
Original Assignee
Nishikawa Rubber Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nishikawa Rubber Co Ltd filed Critical Nishikawa Rubber Co Ltd
Application granted granted Critical
Publication of ATE555196T1 publication Critical patent/ATE555196T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT09169259T 2008-09-03 2009-09-02 Wärmebeständige duplexspezifische nuklease ATE555196T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008226389 2008-09-03
JP2009131942A JP5517284B2 (ja) 2008-09-03 2009-06-01 耐熱性２本鎖特異的核酸分解酵素

Publications (1)

Publication Number Publication Date
ATE555196T1 true ATE555196T1 (de) 2012-05-15

Family

ID=41259451

Family Applications (1)

Application Number Title Priority Date Filing Date
AT09169259T ATE555196T1 (de) 2008-09-03 2009-09-02 Wärmebeständige duplexspezifische nuklease

Country Status (4)

Country Link
US (1) US7951569B2 (de)
EP (1) EP2161331B1 (de)
JP (1) JP5517284B2 (de)
AT (1) ATE555196T1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
WO2013003489A2 (en) * 2011-06-27 2013-01-03 University Of Florida Research Foundation, Inc Method for genome complexity reduction and polymorphism detection
EP3052644B1 (de) * 2013-10-01 2019-07-10 Texcell Detektion von seltenen mikrobiologischen nukleinsäuren
CN103882132A (zh) * 2014-03-27 2014-06-25 中国人民解放军第三军医大学第一附属医院 痕量rna实时动态检测方法
JP2019154396A (ja) * 2018-03-16 2019-09-19 株式会社リコー 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
CN114657202B (zh) * 2022-03-30 2023-10-27 河南省儿童医院郑州儿童医院 一种耐热核酸降解酶表达载体、构建方法及其应用
CN116144630B (zh) * 2022-10-31 2023-10-10 北京擎科生物科技股份有限公司 双链特异性核酸酶突变体及其应用
US20240263158A1 (en) * 2023-02-01 2024-08-08 New England Biolabs, Inc. Duplex-specific dnases
CN120442597B (zh) * 2025-07-11 2025-09-02 江苏愚公生物科技有限公司 一种热敏感双链特异性核酸酶突变体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9716664D0 (en) 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
AU2002346662A1 (en) 2001-12-04 2003-06-17 Euregen Llc Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture
JP2005218385A (ja) * 2004-02-06 2005-08-18 Dnaform:Kk １本鎖ｄｎａを調製するための方法
JP2008226389A (ja) 2007-03-15 2008-09-25 Elpida Memory Inc 半導体記憶装置
JP5134928B2 (ja) 2007-11-30 2013-01-30 浜松ホトニクス株式会社 加工対象物研削方法

Also Published As

Publication number Publication date
EP2161331B1 (de) 2012-04-25
JP2010081934A (ja) 2010-04-15
US7951569B2 (en) 2011-05-31
US20100092976A1 (en) 2010-04-15
EP2161331A1 (de) 2010-03-10
JP5517284B2 (ja) 2014-06-11

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