ATE78870T1 - Verfahren zur herstellung von neutraler protease. - Google Patents

Verfahren zur herstellung von neutraler protease.

Info

Publication number
ATE78870T1
ATE78870T1 AT85830246T AT85830246T ATE78870T1 AT E78870 T1 ATE78870 T1 AT E78870T1 AT 85830246 T AT85830246 T AT 85830246T AT 85830246 T AT85830246 T AT 85830246T AT E78870 T1 ATE78870 T1 AT E78870T1
Authority
AT
Austria
Prior art keywords
neutral protease
production
isolating
hybrid
culture medium
Prior art date
Application number
AT85830246T
Other languages
English (en)
Inventor
Salvatore Toma
Bue Marina Del
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Application granted granted Critical
Publication of ATE78870T1 publication Critical patent/ATE78870T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fish Paste Products (AREA)
AT85830246T 1984-10-17 1985-09-30 Verfahren zur herstellung von neutraler protease. ATE78870T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT23190/84A IT1176997B (it) 1984-10-17 1984-10-17 Procedimento per la produzione di proteasi neutra
EP85830246A EP0179025B1 (de) 1984-10-17 1985-09-30 Verfahren zur Herstellung von neutraler Protease

Publications (1)

Publication Number Publication Date
ATE78870T1 true ATE78870T1 (de) 1992-08-15

Family

ID=11204709

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85830246T ATE78870T1 (de) 1984-10-17 1985-09-30 Verfahren zur herstellung von neutraler protease.

Country Status (6)

Country Link
EP (1) EP0179025B1 (de)
JP (1) JPH0829083B2 (de)
AT (1) ATE78870T1 (de)
CA (1) CA1285233C (de)
DE (1) DE3586410T2 (de)
IT (1) IT1176997B (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0149241B1 (de) * 1983-12-28 1991-03-27 Agency Of Industrial Science And Technology DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
IT1239733B (it) * 1990-02-23 1993-11-15 Eniricerche Spa Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione
CN103859169B (zh) * 2013-12-13 2015-08-12 湖南鸿鹰生物科技有限公司 一种含中性蛋白酶的饲料复合酶及其制备方法
CN103865701B (zh) * 2013-12-13 2016-04-06 湖南鸿鹰生物科技有限公司 一种含中性蛋白酶的啤酒复合酶

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5867181A (ja) * 1981-10-16 1983-04-21 Yakult Honsha Co Ltd スタフイロキナーゼ産生遺伝情報を担うｄｎａ断片組み換え体ｄｎａおよびそれを導入した大腸菌
EP0151760A1 (de) * 1984-01-06 1985-08-21 Takeda Chemical Industries, Ltd. DNA und ihre Verwendung

Also Published As

Publication number Publication date
EP0179025A1 (de) 1986-04-23
DE3586410D1 (de) 1992-09-03
JPS61181374A (ja) 1986-08-14
EP0179025B1 (de) 1992-07-29
CA1285233C (en) 1991-06-25
IT8423190A1 (it) 1986-04-17
JPH0829083B2 (ja) 1996-03-27
IT8423190A0 (it) 1984-10-17
IT1176997B (it) 1987-08-26
DE3586410T2 (de) 1993-03-04

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee