ATE80735T1 - SOLID-SURFACE COLORIMETRIC IMMUNOESSAY, TEST ROD TO BE USED WITH IT AND ITS PREPARATION. - Google Patents

SOLID-SURFACE COLORIMETRIC IMMUNOESSAY, TEST ROD TO BE USED WITH IT AND ITS PREPARATION.

Info

Publication number
ATE80735T1
ATE80735T1 AT84303021T AT84303021T ATE80735T1 AT E80735 T1 ATE80735 T1 AT E80735T1 AT 84303021 T AT84303021 T AT 84303021T AT 84303021 T AT84303021 T AT 84303021T AT E80735 T1 ATE80735 T1 AT E80735T1
Authority
AT
Austria
Prior art keywords
antibody
solid surface
dipstick
antigen
solid
Prior art date
Application number
AT84303021T
Other languages
German (de)
Inventor
Henry Garrett Wada
Sarah R Baxter
Douglas D Rundle
Kenneth C Kasper
John C Lankford
Original Assignee
Quidel Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quidel Corp filed Critical Quidel Corp
Application granted granted Critical
Publication of ATE80735T1 publication Critical patent/ATE80735T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The inventive concept provides an immunoassay using an enzyme label, in which the detection of the unknown substance is performed colorimetrically directly upon a solid surface. Preferably, the surface is an impermeable surface of a dipstick. To detect antigen in a biologically derived fluid sample, a dipstick containing bound antibody may be contacted with the sample and with a soluble enzyme-labeled antibody, to cause the antigen to bind to both the immobilized antibody and the labeled antibody on the surface. After separation of unreacted reagents, the solid surface is contacted with a substrate which converts from a soluble form to an insoluble colored form in the presence of the enzyme and deposits upon the surface of the solid surface. The solid surface is separated from the solution. The sandwich immunoassay technique may also be employed to detect antibody in the sample by reversing the roles of the antigen and antibody. The system is also applicable to a competitive binding technique. The invention further includes a colored dipstick produced by this means.
AT84303021T 1983-05-06 1984-05-04 SOLID-SURFACE COLORIMETRIC IMMUNOESSAY, TEST ROD TO BE USED WITH IT AND ITS PREPARATION. ATE80735T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49240083A 1983-05-06 1983-05-06
EP84303021A EP0125118B1 (en) 1983-05-06 1984-05-04 Colorimetric immunoassay on solid surface, dipstick therefor and its preparation

Publications (1)

Publication Number Publication Date
ATE80735T1 true ATE80735T1 (en) 1992-10-15

Family

ID=23956113

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84303021T ATE80735T1 (en) 1983-05-06 1984-05-04 SOLID-SURFACE COLORIMETRIC IMMUNOESSAY, TEST ROD TO BE USED WITH IT AND ITS PREPARATION.

Country Status (6)

Country Link
EP (1) EP0125118B1 (en)
JP (1) JPS6069554A (en)
AT (1) ATE80735T1 (en)
AU (1) AU2706384A (en)
CA (1) CA1223813A (en)
DE (1) DE3485920T2 (en)

Families Citing this family (38)

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US5622871A (en) 1987-04-27 1997-04-22 Unilever Patent Holdings B.V. Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
IT1213254B (en) * 1984-12-06 1989-12-14 Boehringer Biochemia Srl DIAGNOSTIC METHOD FOR THE EVALUATION OF CLINICAL PARAMETERS BY DIRECT WITHDRAWAL ORGANIC MATERIALS AND DEVICES FOR ITS IMPLEMENTATION.
FI71433C (en) * 1985-05-14 1986-12-19 Orion Yhtymae Oy APPARATUS FOER IMMUNOKEMISK BESTAEMNING
US4720455A (en) * 1985-09-06 1988-01-19 Pitman-Moore, Inc. Progesterone assay method for mammals and monoclonal antibody therefor
US5063151A (en) * 1985-09-20 1991-11-05 Biometallics, Inc. Immunoassay method and kit
US6265176B1 (en) * 1985-10-29 2001-07-24 Cordis Corporation Dot immunoassay on plastic sheets
JPS62170850A (en) * 1986-01-23 1987-07-27 Wako Pure Chem Ind Ltd Immunochemical measurement method
US5206178A (en) * 1986-11-19 1993-04-27 Genetic Systems Corporation Membrane affinity concentration immunoassay
DE3856421T2 (en) 1987-04-27 2000-12-14 Unilever Nv Specific binding test procedures
US6352862B1 (en) 1989-02-17 2002-03-05 Unilever Patent Holdings B.V. Analytical test device for imuno assays and methods of using same
US5252496A (en) 1989-12-18 1993-10-12 Princeton Biomeditech Corporation Carbon black immunochemical label
GB2241329B (en) * 1990-01-10 1994-02-09 Princeton Biomeditech Corp No wash immunoassay
KR910014706A (en) * 1990-01-10 1991-08-31 원본미기재 Improved Immunoassay Device Without Cleaning
KR910014705A (en) * 1990-01-10 1991-08-31 원본미기재 Immunasssay device in the form of a dipstick that does not require cleaning
US5877028A (en) 1991-05-29 1999-03-02 Smithkline Diagnostics, Inc. Immunochromatographic assay device
US5998220A (en) 1991-05-29 1999-12-07 Beckman Coulter, Inc. Opposable-element assay devices, kits, and methods employing them
US6168956B1 (en) 1991-05-29 2001-01-02 Beckman Coulter, Inc. Multiple component chromatographic assay device
US5837546A (en) 1993-08-24 1998-11-17 Metrika, Inc. Electronic assay device and method
US6979576B1 (en) 1997-07-25 2005-12-27 Shu-Ching Cheng Methods of use of one step immunochromatographic device for Streptococcus A antigen
US5879951A (en) 1997-01-29 1999-03-09 Smithkline Diagnostics, Inc. Opposable-element assay device employing unidirectional flow
US5939252A (en) 1997-05-09 1999-08-17 Lennon; Donald J. Detachable-element assay device
US6699722B2 (en) 2000-04-14 2004-03-02 A-Fem Medical Corporation Positive detection lateral-flow apparatus and method for small and large analytes
EP1845375A3 (en) * 2000-12-22 2008-05-21 Bio A.R.T. BVBA Flow through assay device, diagnostic kit comprising said device and use in the detection of an analyte in a sample
US6703216B2 (en) 2002-03-14 2004-03-09 The Regents Of The University Of California Methods, compositions and apparatuses for detection of gamma-hydroxybutyric acid (GHB)
US7150995B2 (en) 2004-01-16 2006-12-19 Metrika, Inc. Methods and systems for point of care bodily fluid analysis
CN101360997B (en) 2005-11-18 2013-05-01 美国政府健康及人类服务部，疾病控制和预防中心 Modified cardiolipin and uses therefor
WO2008154813A1 (en) 2007-06-15 2008-12-24 Xiamen University Monoclonal antibodies binding to avian influenza virus subtype h5 haemagglutinin and uses thereof
WO2011016031A2 (en) 2009-08-03 2011-02-10 Yeda Research And Development Co. Ltd. Urinary biomarkers for cancer diagnosis
US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
CA2836503C (en) 2011-05-23 2020-09-22 Yeda Research And Development Co. Ltd. Use of akt phosphorylation as a biomarker for prognosing neurodegenerative diseases and treating same
EP2812704A4 (en) 2012-02-07 2016-03-23 Intuitive Biosciences Inc KOCH BACILLUS SPECIFIC PEPTIDES FOR THE DETECTION OF INFECTION OR IMMUNIZATION IN NON-HUMAN PRIMATES
CN102798712B (en) * 2012-08-08 2014-06-04 广州市质量监督检测研究院 Fluorescent brightener CBS-X detection kit and preparation method thereof
US20150366944A1 (en) 2013-02-20 2015-12-24 Yeda Research And Development Co. Ltd. Interferon treatment targeting mutant p53 expressing cells
EP3387154A4 (en) 2015-12-09 2019-05-15 Intuitive Biosciences, Inc. AUTOMATED SILVER ENHANCEMENT SYSTEM
EP3820909B1 (en) 2018-07-11 2023-03-01 The United States of America, as represented by the Secretary, Department of Health and Human Services Monoclonal antibody for the detection of the antiretroviral drug emtricitabine (ftc, 2',3'-dideoxy-5-fluoro-3'-thiacytidine)
JP2023506434A (en) 2019-12-11 2023-02-16 イチロヴ テック リミテッド A non-invasive assay to discriminate between bacterial and viral infections
EP4214509A4 (en) 2020-09-15 2025-01-22 Neurodex, Inc. DIAGNOSTIC ASSAYS USING NEURON-DERIVED EXOSOMES
CN119086538B (en) * 2024-09-14 2025-12-05 南开大学 A colorimetric sensor strip for detecting Listeria monocytogenes using metabolic biomarkers, its preparation method and application

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3876504A (en) * 1974-06-10 1975-04-08 Early Warning Co Procedure for determination of antigens and antibodies and articles for use therewith
SE388694B (en) * 1975-01-27 1976-10-11 Kabi Ab WAY TO PROVIDE AN ANTIGEN EXV IN SAMPLES OF BODY WHEATS, USING POROST BERAR MATERIAL BONDED OR ADSORBING ANTIBODIES
WO1980002800A1 (en) * 1979-06-21 1980-12-24 G Oster Specific impending ovulation indicator
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
US4391904A (en) * 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
CH642458A5 (en) * 1980-04-25 1984-04-13 Hoffmann La Roche Immunological method
DE3269567D1 (en) * 1981-04-29 1986-04-10 Ciba Geigy Ag New devices and kits for immunological analysis
US4444880A (en) * 1982-07-27 1984-04-24 Syva Company Periodate removal of ascorbate interference in dipsticks for immunoassays
ES8600404A1 (en) * 1983-05-02 1985-10-01 Enzo Biochem Inc Method and materials useful for the analysis and detection of genetic material.
DE3346795A1 (en) * 1983-12-23 1985-07-04 E. Prof. Dr. 3550 Marburg Geyer Enzyme immunoassay and corresponding diagnostic aid

Also Published As

Publication number Publication date
DE3485920T2 (en) 1993-04-15
EP0125118A2 (en) 1984-11-14
CA1223813A (en) 1987-07-07
DE3485920D1 (en) 1992-10-22
AU2706384A (en) 1984-11-08
EP0125118A3 (en) 1988-01-27
EP0125118B1 (en) 1992-09-16
JPS6069554A (en) 1985-04-20

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