ATE89287T1 - Polymere und deren verwendung bei der elektrophorese. - Google Patents

Polymere und deren verwendung bei der elektrophorese.

Info

Publication number: ATE89287T1
Authority: AT; Austria
Prior art keywords: nat; gels; poly; pct; bis
Prior art date: 1987-06-13

Application number

AT88905199T

Other languages

English (en)

Inventor

Branko Dr Kozulic

Klaus Prof Dr Mosbach

Original Assignee

Kozulic Branko

Mosbach Klaus

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-06-13

Filing date

1988-06-10

Publication date

1993-05-15

1988-06-10 Application filed by Kozulic Branko, Mosbach Klaus filed Critical Kozulic Branko

1993-05-15 Application granted granted Critical

1993-05-15 Publication of ATE89287T1 publication Critical patent/ATE89287T1/de

Links

229920000642 polymer Polymers 0.000 title abstract 2
238000001962 electrophoresis Methods 0.000 title 1
239000000499 gel Substances 0.000 abstract 8
MVBJSQCJPSRKSW-UHFFFAOYSA-N n-[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]prop-2-enamide Chemical compound OCC(CO)(CO)NC(=O)C=C MVBJSQCJPSRKSW-UHFFFAOYSA-N 0.000 abstract 8
239000004971 Cross linker Substances 0.000 abstract 6
229920002401 polyacrylamide Polymers 0.000 abstract 3
230000007717 exclusion Effects 0.000 abstract 2
102000004169 proteins and genes Human genes 0.000 abstract 2
108090000623 proteins and genes Proteins 0.000 abstract 2
239000000959 ampholyte mixture Substances 0.000 abstract 1
230000015572 biosynthetic process Effects 0.000 abstract 1
239000002131 composite material Substances 0.000 abstract 1
239000011544 gradient gel Substances 0.000 abstract 1
238000001155 isoelectric focusing Methods 0.000 abstract 1
ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
-1 poly(NAT) Polymers 0.000 abstract 1
238000006116 polymerization reaction Methods 0.000 abstract 1
238000000926 separation method Methods 0.000 abstract 1
238000003786 synthesis reaction Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/52—Amides or imides
- C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
- C08F220/58—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-(meth)acryloylmorpholine
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S524/00—Synthetic resins or natural rubbers -- part of the class 520 series
- Y10S524/916—Hydrogel compositions

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Physics & Mathematics (AREA)
Organic Chemistry (AREA)
Polymers & Plastics (AREA)
Electrochemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Dispersion Chemistry (AREA)
Immunology (AREA)
Pathology (AREA)
Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Compositions Of Macromolecular Compounds (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Macromonomer-Based Addition Polymer (AREA)
Peptides Or Proteins (AREA)
Graft Or Block Polymers (AREA)
Solid-Sorbent Or Filter-Aiding Compositions (AREA)

AT88905199T 1987-06-13 1988-06-10 Polymere und deren verwendung bei der elektrophorese. ATE89287T1 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
GB878713854A GB8713854D0 (en)	1987-06-13	1987-06-13	Poly-n-acryloyl-tris(hydroxymethyl)aminomethane gels
PCT/EP1988/000515 WO1988009981A2 (en)	1987-06-13	1988-06-10	Polymers, and their use as gels for electrophoresis
EP88905199A EP0318551B1 (de)	1987-06-13	1988-06-10	Polymere und deren verwendung bei der elektrophorese

Publications (1)

Publication Number	Publication Date
ATE89287T1 true ATE89287T1 (de)	1993-05-15

Family

ID=10618866

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT88905199T ATE89287T1 (de)	1987-06-13	1988-06-10	Polymere und deren verwendung bei der elektrophorese.

Country Status (7)

Country	Link
US (1)	US5319046A (de)
EP (1)	EP0318551B1 (de)
JP (1)	JP2654681B2 (de)
AT (1)	ATE89287T1 (de)
DE (1)	DE3880978T2 (de)
GB (2)	GB8713854D0 (de)
WO (1)	WO1988009981A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5238545A (en) *	1991-02-27	1993-08-24	W. R. Grace & Co.-Conn.	Electrophoretic gel for separation and recovery of substances and its use
US5225062A (en) *	1991-02-27	1993-07-06	W. R. Grace & Co. -Conn.	Electrophoretic gel for separation and recovery of substances and its use
US5631337A (en) *	1996-01-19	1997-05-20	Soane Bioscience	Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
US5883211A (en) *	1996-01-19	1999-03-16	Aclara Biosciences, Inc.	Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
US5840877A (en) *	1996-09-05	1998-11-24	Guest Elchrom Scientific	Electrophoresis gels of enhanced selectivity
US6884842B2 (en)	1997-10-14	2005-04-26	Alnis Biosciences, Inc.	Molecular compounds having complementary surfaces to targets
US6117293A (en) *	1998-07-31	2000-09-12	Biowhittaker Molecular Applications, Inc.	Method for producing hydrophilic monomers and uses thereof
US6464850B1 (en)	1998-07-31	2002-10-15	Biowhittaker Molecular Applications, Inc.	Method for producing hydrophilic monomers and uses thereof
SE517422C2 (sv) *	2000-10-06	2002-06-04	Bioglan Ab	Farmaceutiskt acceptabel stärkelse
US7479521B2 (en)	2001-10-17	2009-01-20	Societe D'exploitation De Produits Pour Les Industries Chimiques (Seppic)	Tris(hydroxymethyl) acrylamidomethane polymer, inverse latex and microlatex containing same, use of said polymer, inverse latex and microlatex
FR2830867B1 (fr) *	2001-10-17	2006-12-22	Seppic Sa	Polymere de tris(hydroxymethyl) acrylamidomethane, latex inverse et microlatex en contenant, utilisation desdits polymere, latex inverse et microlatex inverse
US7326324B2 (en) *	2002-11-20	2008-02-05	Shin-Etsu Chemical Co., Ltd.	Purification and use of gellan in electrophoresis gels
US7022214B2 (en) *	2004-01-21	2006-04-04	Bio-Rad Laboratories, Inc.	Carrier ampholytes of high pH range
US7407816B2 (en) *	2004-05-07	2008-08-05	Gentius, Inc	Isoelectric particles and uses thereof
EP2143740B1 (de) †	2004-08-19	2013-06-19	Covidien LP	Wasserquellbare copolymere und damit hergestellte artikel und beschichtung
US10933168B2 (en)	2015-12-04	2021-03-02	Poly-Med, Inc.	Double network hydrogel with anionic polymer and uses therof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US419784A (en) *		1890-01-21		William stoodard mead
DE2159501A1 (de) *	1970-12-09	1972-06-22	Commercial Solvents Corp., Terre Haute, Ind. (V.StA.)	Verfahren zur Herstellung von Bisoder Tris-hydroxymethylamiden
FR2359873A1 (fr) *	1976-07-27	1978-02-24	Sebia Sa	Procede de preparation de gels copolymeres de n-methylol-acrylamide pour la separation des lipoproteines seriques en gradient discontinu
FR2378808A1 (fr) *	1977-01-28	1978-08-25	Mar Pha Etu Expl Marques	Nouveaux copolymeres hydrophiles a base de n-(tris(hydroxy-methyl)methyl)acrylamide ou de n-(tris (hydroxymethyl)methyl)methacrylamide, leur preparation et leur emploi dans les techniques de separation
FR2482112B1 (fr) *	1980-05-09	1985-06-07	Pharmindustrie	Nouveaux copolymeres hydrophiles a base de n-(tris (hydroxymethyl) methyl) acrylamide, procedes pour leur preparation, gels aqueux desdits copolymeres et leur utilisation comme echangeurs d'ions
US4806434A (en) *	1984-07-06	1989-02-21	Fuji Photo Film Co., Ltd.	Medium for electrophoresis

1987
- 1987-06-13 GB GB878713854A patent/GB8713854D0/en active Pending
1988
- 1988-06-10 AT AT88905199T patent/ATE89287T1/de active
- 1988-06-10 US US07/328,123 patent/US5319046A/en not_active Expired - Lifetime
- 1988-06-10 DE DE88905199T patent/DE3880978T2/de not_active Expired - Lifetime
- 1988-06-10 JP JP63505008A patent/JP2654681B2/ja not_active Expired - Lifetime
- 1988-06-10 EP EP88905199A patent/EP0318551B1/de not_active Expired - Lifetime
- 1988-06-10 GB GB08813744A patent/GB2206594A/en not_active Withdrawn
- 1988-06-10 WO PCT/EP1988/000515 patent/WO1988009981A2/en not_active Ceased

Also Published As

Publication number	Publication date
GB8813744D0 (en)	1988-07-13
JP2654681B2 (ja)	1997-09-17
EP0318551B1 (de)	1993-05-12
WO1988009981A3 (en)	1989-02-09
EP0318551A1 (de)	1989-06-07
GB2206594A (en)	1989-01-11
JPH01503629A (ja)	1989-12-07
GB8713854D0 (en)	1987-07-15
WO1988009981A2 (en)	1988-12-15
DE3880978T2 (de)	1993-10-14
DE3880978D1 (de)	1993-06-17
US5319046A (en)	1994-06-07

Similar Documents

Publication	Publication Date	Title
ATE89287T1 (de)	1993-05-15	Polymere und deren verwendung bei der elektrophorese.
Takács	1979	Electrophoresis of proteins in polyacrylamide slab gels
McDonagh et al.	1972	Molecular weight analysis of fibrinogen and fibrin chains by an improved sodium dodecyl sulfate gel electrophoresis method
CA2125136C (en)	2003-12-30	New formulations for polyacrylamide matrices in electrokinetic and chromatographic methodologies
Goldenberg et al.	1984	Gel electrophoresis in studies of protein conformation and folding
Martini et al.	1971	Enumeration of rabbit reticulocyte ribosomal proteins
US4130470A (en)	1978-12-19	Method for generating a pH-function for use in electrophoresis
Altland et al.	1985	Hybridisoelectric focusing in rehydrated immobilized pH gradients with added carrier ampholytes: Demonstration of human globins
DE69429338D1 (de)	2002-01-17	Nicht-nucleosidische cumarin-derivate als reagenzien zum crosslinking von polynucleotiden
DE3587394D1 (de)	1993-07-15	Rekombinante virusproteine begleitet von lymphadenopathischem syndrom und/oder "acquired immune deficiency syndrome" (aids).
ATE277949T1 (de)	2004-10-15	Klärung von protein-niederschlagsuspensionen durch verwendung von anionischen polymerischen flockungsmitteln
GB2267502B (en)	1997-01-22	Polymeric reaction products for use in immobilized buffered gels and membranes
Rabilloud et al.	1987	Protein precipitation induced by alkaline Immobilines for isoelectric focusing in immobilized pH gradients: Causes and remedies
Bothe et al.	1985	A sodium dodecyl sulfate-gradient gel electrophoresis system that separates polypeptides in the molecular weight range of 1500 to 100,000
Arnaud et al.	1975	The Pi system: its study by means of thin-layer-gel electrofocusing in polyacrylamide gel
Manabe et al.	1983	Analysis of human haptoglobin‐hemoglobin complexes by micro two‐dimensional electrophoresis
Stoklosa et al.	1974	Molecular weight determinations of proteins by polyacrylamide gel electrophoresis with sodium dodecyl sulfate in just the sample solution
Righetti et al.	1989	Isoelectric focusing in immobilized pH gradients
Otavsky et al.	1977	Isoelectric focusing in Pevikon slabs
Chen et al.	1981	Affinity electrophoresis in gels containing hydrophobic substituents.
Jones et al.	1981	A method for the preparation of low-pH dodecyl sulphate/polyacrylamide-gradient gels
Perego et al.	1997	Separation of oligonucleotides of identical size, but different base composition, by free zone capillary electrophoresis in strongly acidic, isoelectric buffers
Gianazza et al.	1980	Isoelectric focusing followed by electrophoresis of protein for visualizing their titration curves by zymogram and immunofixation
Barbehenn et al.	1982	Characterization of polynucleotide phosphorylase from Micrococcus luteus and isolation of the 13,000 base poly (A) product of the polymerization reaction.
Pernelle et al.	1986	High‐resolution two‐dimensional electrophoresis of myofibrillar proteins with immobilized pH gradients