ATE92534T1 - Verfahren zur trennung von boesartigen zellen aus einer klinischen probe. - Google Patents

Verfahren zur trennung von boesartigen zellen aus einer klinischen probe.

Info

Publication number
ATE92534T1
ATE92534T1 AT88300969T AT88300969T ATE92534T1 AT E92534 T1 ATE92534 T1 AT E92534T1 AT 88300969 T AT88300969 T AT 88300969T AT 88300969 T AT88300969 T AT 88300969T AT E92534 T1 ATE92534 T1 AT E92534T1
Authority
AT
Austria
Prior art keywords
fragments
cells
medium
tumor
serum
Prior art date
Application number
AT88300969T
Other languages
English (en)
Inventor
Boris M Rotman
Original Assignee
Analytical Biosystems Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Analytical Biosystems Corp filed Critical Analytical Biosystems Corp
Application granted granted Critical
Publication of ATE92534T1 publication Critical patent/ATE92534T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y10S436/813—Cancer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT88300969T 1987-02-05 1988-02-05 Verfahren zur trennung von boesartigen zellen aus einer klinischen probe. ATE92534T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/011,219 US4937187A (en) 1983-02-04 1987-02-05 Methods for separating malignant cells from clinical specimens
EP88300969A EP0277837B1 (de) 1987-02-05 1988-02-05 Verfahren zur Trennung von bösartigen Zellen aus einer klinischen Probe

Publications (1)

Publication Number Publication Date
ATE92534T1 true ATE92534T1 (de) 1993-08-15

Family

ID=21749375

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88300969T ATE92534T1 (de) 1987-02-05 1988-02-05 Verfahren zur trennung von boesartigen zellen aus einer klinischen probe.

Country Status (6)

Country Link
US (1) US4937187A (de)
EP (1) EP0277837B1 (de)
JP (1) JPS63263081A (de)
AT (1) ATE92534T1 (de)
CA (1) CA1293216C (de)
DE (1) DE3882725T2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242806A (en) * 1990-05-07 1993-09-07 Baxter Diagnostics Inc. Method for conducting the cytotoxicity assays on tumor cells
US5962317A (en) * 1995-10-18 1999-10-05 The Johns Hopkins University School Of Medicine Dosage modeling system
US6287848B1 (en) 1995-10-18 2001-09-11 Johns Hopkins University Dosage modeling system
US20040072722A1 (en) 2002-10-10 2004-04-15 Kornblith Paul L. Methods for assessing efficacy of chemotherapeutic agents
US6416967B2 (en) * 1996-07-12 2002-07-09 Precision Therapeutics, Inc. Method of using multicellular particulates to analyze malignant or hyperproliferative tissue
US5728541A (en) * 1996-07-12 1998-03-17 Precision Therapeutics, Inc. Method for preparing cell cultures from biologial specimens for chemotherapeutic and other assays
US20040023375A1 (en) * 2002-07-30 2004-02-05 Precision Therapeutics, Inc. Method for preparing cell cultures from biological specimens for chemotherapeutic and other assays
US6316215B1 (en) 1999-12-27 2001-11-13 Edwin L. Adair Methods of cancer screening utilizing fluorescence detection techniques and selectable imager charge integration periods
FR2782730B1 (fr) * 1998-08-25 2002-05-17 Biocom Sa Procede de separation cellulaire pour l'isolation de cellules pathogeniques, notamment cancereuses rares, equipement et reactif pour la mise en oeuvre du procede et application du procede
US6190877B1 (en) * 1999-12-27 2001-02-20 Edwin L. Adair Method of cancer screening primarily utilizing non-invasive cell collection and fluorescence detection techniques
US6984498B2 (en) 1999-12-27 2006-01-10 Adair Edwin L Method of cancer screening primarily utilizing non-invasive cell collection, fluorescence detection techniques, and radio tracing detection techniques
US6750037B2 (en) 1999-12-27 2004-06-15 Edwin L. Adair Method of cancer screening primarily utilizing non-invasive cell collection, fluorescence detection techniques, and radio tracing detection techniques
US7785810B2 (en) 2000-09-09 2010-08-31 The Research Foundation Of State University Of New York Method and compositions for isolating metastatic cancer cells, and use in measuring metastatic potential of a cancer thereof
US20040086888A1 (en) * 2001-10-18 2004-05-06 Kornblith Paul L Method for tandem genomic/proteomic analysis of proliferating cells
EP1374912B1 (de) 2002-06-21 2006-08-16 Edwin L. Adair Anwendung von Metalloporphyrinen zur Behandlung von Arteriosklerose
CA2620936A1 (en) * 2005-09-01 2007-03-08 Precision Therapeutics, Inc. Chemo-sensitivity assays using tumor cells exhibiting persistent phenotypic characteristics
DE102012003700B3 (de) * 2012-02-28 2012-11-29 FLACOD GmbH Verfahren zur Trennung von Verbänden maligner Zellen und Verbänden aus Stromazellen einer Malignomgewebeprobe

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3073746A (en) * 1959-05-21 1963-01-15 Organon Process for culturing altered human secretory pituitary cells
US3586859A (en) * 1970-02-16 1971-06-22 Irwin J Katz Fluorescent cell viability counter
GB1376131A (en) * 1971-02-09 1974-12-04 Nat Res Dev Improvements in and relating to cell culture systems
US3821087A (en) * 1972-05-18 1974-06-28 Dedrick R Cell culture on semi-permeable tubular membranes
US3948732A (en) * 1973-05-31 1976-04-06 Instrumentation Laboratory, Inc. Cell culture assembly
US4201845A (en) * 1976-04-12 1980-05-06 Monsanto Company Cell culture reactor
US4184922A (en) * 1977-11-11 1980-01-22 The Government Of The United States Dual circuit, woven artificial capillary bundle for cell culture
US4220725A (en) * 1978-04-03 1980-09-02 United States Of America Capillary cell culture device
US4242459A (en) * 1978-11-02 1980-12-30 Chick William L Cell culture device
US4241187A (en) * 1979-03-27 1980-12-23 United States Of America Method and apparatus for cell and tissue culture
JPS5642584A (en) * 1979-09-18 1981-04-20 Asahi Chem Ind Co Ltd Cell cultivation method
US4308351A (en) * 1980-04-18 1981-12-29 Joseph Leighton System for growing tissue cultures
US4332893A (en) * 1980-06-13 1982-06-01 Rosenberg Ralph A Process for the production of an insulin-producing cell line of pancreatic beta cells
US4350768A (en) * 1980-09-19 1982-09-21 Bristol Myers Company Method for preparing single cell suspension
US4423145A (en) * 1981-05-07 1983-12-27 Stampfer Martha R Enhanced growth medium and method for culturing human mammary epithelial cells
CA1178183A (en) * 1981-05-14 1984-11-20 David J. Zahniser Disaggregation device for cell suspensions
US4537860A (en) * 1982-12-08 1985-08-27 Monsanto Company Static cell culture maintenance system
US4582787A (en) * 1982-12-30 1986-04-15 Frankel Jack W Method of testing a patient for a predisposition to lung cancer, certain other cancers, neurofibromatosis and certain other hereditary disorders
US4559299A (en) * 1983-02-04 1985-12-17 Brown University Research Foundation Inc. Cytotoxicity assays in cell culturing devices
US4734372A (en) * 1983-02-04 1988-03-29 Brown University Research Foundation Cell culturing methods and apparatus
US4554251A (en) * 1983-11-07 1985-11-19 The Ohio State University Research Foundation Process and medium for culturing ant venom gland cells

Also Published As

Publication number Publication date
EP0277837A2 (de) 1988-08-10
EP0277837A3 (en) 1989-12-06
EP0277837B1 (de) 1993-08-04
DE3882725T2 (de) 1994-02-17
JPS63263081A (ja) 1988-10-31
DE3882725D1 (de) 1993-09-09
CA1293216C (en) 1991-12-17
US4937187A (en) 1990-06-26

Similar Documents

Publication Publication Date Title
ATE92534T1 (de) Verfahren zur trennung von boesartigen zellen aus einer klinischen probe.
Yen et al. Isolation of multipotent cells from human term placenta
KR20030069115A (ko) 제대혈 유래 간엽줄기세포ㆍ전구세포의 분리배양방법 및간엽조직으로의 분화유도방법
Bai et al. Comparison of human amniotic fluid-derived and umbilical cord Wharton's Jelly-derived mesenchymal stromal cells: Characterization and myocardial differentiation capacity
DE60019111D1 (de) Verfahren zur zellseparation unter verwendung von immunorosetten
CN110055222B (zh) 包被液和原代肿瘤细胞的分离培养方法
Shi et al. Differentiation capacity of human urine-derived stem cells to retain telomerase activity
Kit-Anan et al. Multiplexing physical stimulation on single human induced pluripotent stem cell-derived cardiomyocytes for phenotype modulation
CN101531996B (zh) 源于成形组织的间充质干细胞的分离和纯化方法
ATE530657T1 (de) Verfahren zur herstellung von zellinien mit latentem immunschwächevirus
JP2009517079A (ja) Ｏｃｔ４発現能を有する臍帯血由来の多分化能性成体幹細胞及びその製造方法
Feng et al. A small-molecule cocktails-based strategy in culture of mesenchymal stem cells
CN106434852A (zh) 一种基于手性自组装纳米传感器实现胞内端粒酶活性检测的方法
ES2001864A6 (es) Un metodo de determinacion de la presencia de anticuerpos contra las glicoproteinas ga, gb y gc de vzv y un dispositivo de diagnostico
CN115161283A (zh) 一种用于肝门部胆管癌来源类器官定向分化和培养的组合物及应用
CN113106058A (zh) 一种人脐带来源Muse细胞的筛选与鉴定方法
McMillan et al. Hepatocyte cell surface polarity as demonstrated by lectin binding.
CN101418284A (zh) 分离纯化人羊水干细胞的方法
NZ509451A (en) Assay for the presence or quantity of a hormone, lipid, nucleic acid or enzyme in a mammal to indicate the presence of non-fertilisable ova capable of in vitro maturation are present
CN111979177A (zh) 一种人胆管上皮细胞的制备方法及其培养基
Anastasiu et al. Explants-isolated human placenta and umbilical cord cells share characteristics of both epithelial and mesenchymal stem cells
CN112755027A (zh) 次乌头碱及其结构类似物作为Sirt3抑制剂和诱导体内外氧化应激的应用
FI871830A0 (fi) Immobilisering av celler som inte fastnar vid en baerare.
JPS5515746A (en) Preparation of l-alpha-glycerophosphoric acid oxidase
US4933278A (en) Method of determining the number of cells in cell culture

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties