AU1523799A - Methods and compositions for detection of specific nucleotide sequences - Google Patents
Methods and compositions for detection of specific nucleotide sequences Download PDFInfo
- Publication number
- AU1523799A AU1523799A AU15237/99A AU1523799A AU1523799A AU 1523799 A AU1523799 A AU 1523799A AU 15237/99 A AU15237/99 A AU 15237/99A AU 1523799 A AU1523799 A AU 1523799A AU 1523799 A AU1523799 A AU 1523799A
- Authority
- AU
- Australia
- Prior art keywords
- target
- probe
- nucleic acid
- acid sequence
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims description 196
- 239000000203 mixture Substances 0.000 title claims description 74
- 108091028043 Nucleic acid sequence Proteins 0.000 title claims description 37
- 238000001514 detection method Methods 0.000 title description 67
- 239000000523 sample Substances 0.000 claims description 584
- 150000007523 nucleic acids Chemical group 0.000 claims description 162
- 238000009396 hybridization Methods 0.000 claims description 77
- 230000000295 complement effect Effects 0.000 claims description 33
- 125000003729 nucleotide group Chemical group 0.000 claims description 22
- 238000005520 cutting process Methods 0.000 claims description 18
- 230000015572 biosynthetic process Effects 0.000 claims description 13
- 230000004913 activation Effects 0.000 claims description 7
- LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 claims description 5
- 150000003230 pyrimidines Chemical class 0.000 claims description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims 2
- 230000003213 activating effect Effects 0.000 claims 1
- 102000053602 DNA Human genes 0.000 description 169
- 108020004414 DNA Proteins 0.000 description 168
- 102000039446 nucleic acids Human genes 0.000 description 109
- 108020004707 nucleic acids Proteins 0.000 description 109
- 238000003556 assay Methods 0.000 description 75
- 108020004999 messenger RNA Proteins 0.000 description 71
- 239000000499 gel Substances 0.000 description 62
- AGFIRQJZCNVMCW-UAKXSSHOSA-N 5-bromouridine Chemical class O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 AGFIRQJZCNVMCW-UAKXSSHOSA-N 0.000 description 40
- 238000003199 nucleic acid amplification method Methods 0.000 description 40
- 230000003321 amplification Effects 0.000 description 39
- 230000027455 binding Effects 0.000 description 39
- 238000004458 analytical method Methods 0.000 description 35
- 238000002955 isolation Methods 0.000 description 31
- 238000005516 engineering process Methods 0.000 description 27
- 239000011324 bead Substances 0.000 description 25
- 101710163270 Nuclease Proteins 0.000 description 21
- 238000003776 cleavage reaction Methods 0.000 description 21
- 230000007017 scission Effects 0.000 description 21
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 20
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 20
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 20
- 108090000623 proteins and genes Proteins 0.000 description 20
- 230000035945 sensitivity Effects 0.000 description 20
- 238000012360 testing method Methods 0.000 description 19
- 239000000872 buffer Substances 0.000 description 18
- 239000012528 membrane Substances 0.000 description 18
- 238000000926 separation method Methods 0.000 description 18
- 238000011282 treatment Methods 0.000 description 17
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 16
- 239000002773 nucleotide Substances 0.000 description 16
- 239000012634 fragment Substances 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 13
- 208000015181 infectious disease Diseases 0.000 description 13
- 239000007787 solid Substances 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 108060002716 Exonuclease Proteins 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 12
- 102000013165 exonuclease Human genes 0.000 description 12
- 238000005755 formation reaction Methods 0.000 description 12
- 230000035772 mutation Effects 0.000 description 12
- 239000000758 substrate Substances 0.000 description 12
- 238000012546 transfer Methods 0.000 description 12
- 238000005406 washing Methods 0.000 description 12
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 11
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 11
- 230000008901 benefit Effects 0.000 description 11
- 238000013508 migration Methods 0.000 description 11
- 230000005012 migration Effects 0.000 description 11
- 108091008146 restriction endonucleases Proteins 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 230000008569 process Effects 0.000 description 9
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical class CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 9
- -1 uracil free radical Chemical class 0.000 description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 8
- 241000282412 Homo Species 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 229960002685 biotin Drugs 0.000 description 8
- 235000020958 biotin Nutrition 0.000 description 8
- 239000011616 biotin Substances 0.000 description 8
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 8
- 229910052794 bromium Inorganic materials 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 238000001962 electrophoresis Methods 0.000 description 8
- 230000002458 infectious effect Effects 0.000 description 8
- 150000003254 radicals Chemical class 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 241001515965 unidentified phage Species 0.000 description 8
- 238000012800 visualization Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 102100031780 Endonuclease Human genes 0.000 description 7
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 230000029087 digestion Effects 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 239000002853 nucleic acid probe Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- 108010042407 Endonucleases Proteins 0.000 description 6
- 241000725303 Human immunodeficiency virus Species 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 6
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 6
- 239000003593 chromogenic compound Substances 0.000 description 6
- 230000006378 damage Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 238000001502 gel electrophoresis Methods 0.000 description 6
- 238000001727 in vivo Methods 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000002560 therapeutic procedure Methods 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 5
- 208000035473 Communicable disease Diseases 0.000 description 5
- 102000004678 Exoribonucleases Human genes 0.000 description 5
- 108010002700 Exoribonucleases Proteins 0.000 description 5
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 5
- 238000000636 Northern blotting Methods 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 230000029918 bioluminescence Effects 0.000 description 5
- 238000005415 bioluminescence Methods 0.000 description 5
- 239000010836 blood and blood product Substances 0.000 description 5
- 238000011109 contamination Methods 0.000 description 5
- 238000004925 denaturation Methods 0.000 description 5
- 230000036425 denaturation Effects 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 239000000975 dye Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000012340 reverse transcriptase PCR Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 4
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 4
- 108020003215 DNA Probes Proteins 0.000 description 4
- 238000000018 DNA microarray Methods 0.000 description 4
- 239000003298 DNA probe Substances 0.000 description 4
- 208000031886 HIV Infections Diseases 0.000 description 4
- 208000026350 Inborn Genetic disease Diseases 0.000 description 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 4
- 229960000643 adenine Drugs 0.000 description 4
- 238000002820 assay format Methods 0.000 description 4
- 229940125691 blood product Drugs 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 208000016361 genetic disease Diseases 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 241000193738 Bacillus anthracis Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 3
- 108010006654 Bleomycin Proteins 0.000 description 3
- 102000053642 Catalytic RNA Human genes 0.000 description 3
- 108090000994 Catalytic RNA Proteins 0.000 description 3
- 102000004533 Endonucleases Human genes 0.000 description 3
- 108010067770 Endopeptidase K Proteins 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 108091093078 Pyrimidine dimer Proteins 0.000 description 3
- 108010083644 Ribonucleases Proteins 0.000 description 3
- 102000006382 Ribonucleases Human genes 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 3
- 229960001561 bleomycin Drugs 0.000 description 3
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 241001493065 dsRNA viruses Species 0.000 description 3
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 239000006249 magnetic particle Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 108091092562 ribozyme Proteins 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000004513 sizing Methods 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 229940104230 thymidine Drugs 0.000 description 3
- 108700012359 toxins Proteins 0.000 description 3
- 230000014616 translation Effects 0.000 description 3
- KBRISHLGLPXESO-UHFFFAOYSA-N 7h-purine;pyrimidine Chemical group C1=CN=CN=C1.C1=CN=CN=C1.C1=NC=C2NC=NC2=N1 KBRISHLGLPXESO-UHFFFAOYSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 244000153158 Ammi visnaga Species 0.000 description 2
- 235000010585 Ammi visnaga Nutrition 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 2
- 108020004491 Antisense DNA Proteins 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 2
- 239000004971 Cross linker Substances 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 108010086093 Mung Bean Nuclease Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003570 air Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 239000003816 antisense DNA Substances 0.000 description 2
- 229940065181 bacillus anthracis Drugs 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000002306 biochemical method Methods 0.000 description 2
- 238000005460 biophysical method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000013256 coordination polymer Substances 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 230000006862 enzymatic digestion Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000198 fluorescence anisotropy Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000007901 in situ hybridization Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 210000001165 lymph node Anatomy 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000012372 quality testing Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- HERSSAVMHCMYSQ-UHFFFAOYSA-N 1,8-diazacyclotetradecane-2,9-dione Chemical compound O=C1CCCCCNC(=O)CCCCCN1 HERSSAVMHCMYSQ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- ZFTBZKVVGZNMJR-UHFFFAOYSA-N 5-chlorouracil Chemical compound ClC1=CNC(=O)NC1=O ZFTBZKVVGZNMJR-UHFFFAOYSA-N 0.000 description 1
- LTUANIXWARRVBW-UHFFFAOYSA-N 6-(bromoamino)-1h-pyrimidin-2-one Chemical compound BrNC1=CC=NC(=O)N1 LTUANIXWARRVBW-UHFFFAOYSA-N 0.000 description 1
- NODIYIBFQSQLEU-UHFFFAOYSA-N 6-(chloroamino)-1h-pyrimidin-2-one Chemical compound ClNC1=CC=NC(=O)N1 NODIYIBFQSQLEU-UHFFFAOYSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 108010000239 Aequorin Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 241001227713 Chiron Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 230000007023 DNA restriction-modification system Effects 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 241001534160 Escherichia virus Qbeta Species 0.000 description 1
- 241000724791 Filamentous phage Species 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 1
- 108010074633 Mixed Function Oxygenases Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Natural products O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000036436 anti-hiv Effects 0.000 description 1
- 229940045686 antimetabolites antineoplastic purine analogs Drugs 0.000 description 1
- 229940045688 antineoplastic antimetabolites pyrimidine analogues Drugs 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000001818 capillary gel electrophoresis Methods 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001085 differential centrifugation Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical group 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000003358 metastasis assay Methods 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 208000017983 photosensitivity disease Diseases 0.000 description 1
- 231100000434 photosensitization Toxicity 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 101150079601 recA gene Proteins 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000010583 slow cooling Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000007794 visualization technique Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6537897P | 1997-11-12 | 1997-11-12 | |
| US60065378 | 1997-11-12 | ||
| US7581298P | 1998-02-24 | 1998-02-24 | |
| US60075812 | 1998-02-24 | ||
| US7687298P | 1998-03-05 | 1998-03-05 | |
| US60076872 | 1998-03-05 | ||
| PCT/US1998/024226 WO1999024621A2 (fr) | 1997-11-12 | 1998-11-12 | Methodes et compositions de detection de sequences nucleotidiques specifiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU1523799A true AU1523799A (en) | 1999-05-31 |
Family
ID=27370777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU15237/99A Abandoned AU1523799A (en) | 1997-11-12 | 1998-11-12 | Methods and compositions for detection of specific nucleotide sequences |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1030936A2 (fr) |
| JP (1) | JP2001522588A (fr) |
| KR (1) | KR20010032036A (fr) |
| AU (1) | AU1523799A (fr) |
| CA (1) | CA2309861A1 (fr) |
| MX (1) | MXPA00004675A (fr) |
| WO (1) | WO1999024621A2 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100438174B1 (ko) * | 2001-09-05 | 2004-07-01 | 엘지전자 주식회사 | 동기식 이동통신시스템에서의 데이터 전송속도 제어방법 |
| GB2517700A (en) * | 2013-08-27 | 2015-03-04 | Lgc Ltd | Oligonucleotides comprising a secondary structure and uses thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11513887A (ja) * | 1995-10-27 | 1999-11-30 | ランバーグ，エリオット，アール． | 特定のヌクレオチド配列の検出方法および組成物 |
-
1998
- 1998-11-12 AU AU15237/99A patent/AU1523799A/en not_active Abandoned
- 1998-11-12 KR KR1020007005156A patent/KR20010032036A/ko not_active Ceased
- 1998-11-12 EP EP98959441A patent/EP1030936A2/fr not_active Withdrawn
- 1998-11-12 MX MXPA00004675A patent/MXPA00004675A/es unknown
- 1998-11-12 JP JP2000519613A patent/JP2001522588A/ja active Pending
- 1998-11-12 WO PCT/US1998/024226 patent/WO1999024621A2/fr not_active Ceased
- 1998-11-12 CA CA002309861A patent/CA2309861A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999024621A3 (fr) | 1999-08-19 |
| KR20010032036A (ko) | 2001-04-16 |
| JP2001522588A (ja) | 2001-11-20 |
| WO1999024621A2 (fr) | 1999-05-20 |
| EP1030936A2 (fr) | 2000-08-30 |
| CA2309861A1 (fr) | 1999-05-20 |
| WO1999024621A9 (fr) | 2001-05-31 |
| MXPA00004675A (es) | 2004-03-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4216333B2 (ja) | オリゴヌクレオチドの化学結合による核酸検出及び増幅 | |
| EP0707076B1 (fr) | Procédé pour la détermination d'un analyte utilisant une sonde qui contient une matrice d'ARN réplicable | |
| US5093245A (en) | Labeling by simultaneous ligation and restriction | |
| JP2788034B2 (ja) | ポリヌクレオチドのアツセイのための増幅方法 | |
| JP3778925B2 (ja) | 高感度核酸サンドイッチハイブリダイゼーション検定法及びキット | |
| CA2537810C (fr) | Dosage de detection d'acide nucleique | |
| JP4108741B2 (ja) | Ｒｎａバイナリ・プローブとｒｎａ向けｒｎａリガーゼを使用したｒｎａの診断検定法およびそのキット | |
| JP2005511030A (ja) | 核酸の増幅方法 | |
| AU741141B2 (en) | Specific and sensitive method for detecting nucleic acids | |
| JPH02503054A (ja) | 核酸配列の増幅および検出 | |
| JP2002505117A (ja) | チモーゲン性核酸検出方法、および関連分子およびキット | |
| EP0530998B1 (fr) | Détection de sequences nucléotidiques complémentaires | |
| Dahlén et al. | The use of europium (Eu3+) labelled primers in PCR amplification of specific target DNA | |
| AU1523799A (en) | Methods and compositions for detection of specific nucleotide sequences | |
| Stefano et al. | Rapid and sensitive detection ofChlamydia trachomatisusing a ligatable binary RNA probe and Qβ replicase | |
| Kalisch | Patent Number: 5,312,728 May 17, 1994 Date of Patent | |
| Sweden et al. | Lizardi et al. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK5 | Application lapsed section 142(2)(e) - patent request and compl. specification not accepted |