AU2001296830A1 - Ligand based solution assay for low concentration analytes - Google Patents

Ligand based solution assay for low concentration analytes

Info

Publication number
AU2001296830A1
AU2001296830A1 AU2001296830A AU9683001A AU2001296830A1 AU 2001296830 A1 AU2001296830 A1 AU 2001296830A1 AU 2001296830 A AU2001296830 A AU 2001296830A AU 9683001 A AU9683001 A AU 9683001A AU 2001296830 A1 AU2001296830 A1 AU 2001296830A1
Authority
AU
Australia
Prior art keywords
low concentration
based solution
ligand based
solution assay
concentration analytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001296830A
Inventor
Alex M. Saunders
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU2001296830A1 publication Critical patent/AU2001296830A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The present invention provides an assay for an analyte in solution. The assay relies, in part, on associating two catalytic activities in close proximity to each other (e.g., by conjugating each enzyme to a ligand that binds the analyte) and providing substrates that produce a colored product only when both activities are bound to the same molecule in solution.
AU2001296830A 2000-10-14 2001-10-12 Ligand based solution assay for low concentration analytes Abandoned AU2001296830A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US24044200P 2000-10-14 2000-10-14
US60240442 2000-10-14
US24406500P 2000-10-28 2000-10-28
US60244065 2000-10-28
PCT/US2001/031939 WO2002033396A1 (en) 2000-10-14 2001-10-12 Ligand based solution assay for low concentration analytes

Publications (1)

Publication Number Publication Date
AU2001296830A1 true AU2001296830A1 (en) 2002-04-29

Family

ID=26933415

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001296830A Abandoned AU2001296830A1 (en) 2000-10-14 2001-10-12 Ligand based solution assay for low concentration analytes

Country Status (6)

Country Link
US (2) US6682903B2 (en)
EP (1) EP1332357B1 (en)
AT (1) ATE327509T1 (en)
AU (1) AU2001296830A1 (en)
DE (1) DE60119958T2 (en)
WO (1) WO2002033396A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4606543B2 (en) * 2000-04-13 2011-01-05 パナソニック株式会社 Method for confirming amount of solution to be measured and measuring system control method in optical property measuring apparatus
US20050191704A1 (en) * 2004-03-01 2005-09-01 Kimberly-Clark Worldwide, Inc. Assay devices utilizing chemichromic dyes
EP1745129B1 (en) * 2004-04-16 2009-04-15 Idexx Laboratories, Inc. Antibodies against canine pancreatic lipase
AU2005238549B2 (en) * 2004-04-30 2009-12-24 Enterix Pty. Limited Device and method for detecting the presence of hemoglobin in a biological sample
US7094528B2 (en) * 2004-06-30 2006-08-22 Kimberly-Clark Worldwide, Inc. Magnetic enzyme detection techniques
US7521226B2 (en) * 2004-06-30 2009-04-21 Kimberly-Clark Worldwide, Inc. One-step enzymatic and amine detection technique
US7906276B2 (en) 2004-06-30 2011-03-15 Kimberly-Clark Worldwide, Inc. Enzymatic detection techniques
US20060223052A1 (en) * 2005-03-30 2006-10-05 Kimberly-Clark Worldwide, Inc. Technique for detecting microorganisms
EP2745114B1 (en) * 2011-09-16 2018-10-17 Credo Biomedical Pte Ltd. Molecular diagnostic assay device and method of use
WO2013095137A2 (en) * 2011-12-23 2013-06-27 Rijksuniversiteit Groningen Methods, means and kits for detecting scarce analytes
CN105102696B (en) * 2013-02-08 2017-08-15 生物辐射实验室股份有限公司 Affinity-Based Partitioning Assays for Target Molecule Detection
CN113906286B (en) * 2019-07-02 2024-09-20 株式会社堀场先进技术 Biological sample analysis device and biological sample analysis method

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3975237A (en) 1972-11-06 1976-08-17 Syva Company Compounds for enzyme amplification assay - - ecgonine analogs
US4474878A (en) * 1975-09-29 1984-10-02 Cordis Laboratories, Inc. Sandwich EIA for antigen associated with hepatitis
FR2460663A1 (en) 1979-07-10 1981-01-30 Oreal TINCTORIAL COMPOSITION FOR HAIR BASED ON PARAPHENYLENE DIAMINES
JPS5963198A (en) * 1982-10-01 1984-04-10 Toyo Jozo Co Ltd Quantitative assay using enzyme
US4687735A (en) * 1983-03-14 1987-08-18 Syntex (U.S.A.) Inc. Enzymatic poly-reactant channeling binding assay
US4810631A (en) * 1986-05-12 1989-03-07 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of chemical catalysis
US4835099A (en) 1986-11-20 1989-05-30 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of enzymatic catalysis
US4904583A (en) 1987-05-26 1990-02-27 Becton, Dickinson And Company Cascade immunoassay by multiple binding reactions
US5196306A (en) 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
US5183941A (en) 1989-05-18 1993-02-02 Clairol Incorporated Hair dye coupler compounds
EP0496793B1 (en) 1989-10-17 1995-12-20 British Technology Group Ltd Enhanced chemiluminescent assay
WO1991015767A1 (en) * 1990-04-06 1991-10-17 University Of Florida Liquid-phase immunodiagnostic assay
US5100436A (en) 1991-03-07 1992-03-31 Clairol Incorporated Oxidative hair dyeing process with catalytic pretreatment
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
WO1994029722A1 (en) * 1993-06-08 1994-12-22 Chronomed, Inc. Two-phase optical assay method and apparatus
FR2708286B1 (en) * 1993-07-28 1995-10-20 Rambach Alain Method of identifying microorganisms with at least two chromogens.
US5843666A (en) 1994-09-02 1998-12-01 Lumigen, Inc. Chemiluminescent detection methods using dual enzyer-labeled binding partners
DE19622458C2 (en) 1996-05-24 1998-03-26 Senslab Ges Zur Entwicklung Un Enzymatic-electrochemical one-step affinity sensor for the quantitative determination of analytes in aqueous media and affinity assay
DE19629142A1 (en) 1996-07-19 1998-01-22 Agfa Gevaert Ag Colour photographic recording material for negative or reversal film
WO1998022822A1 (en) 1996-11-21 1998-05-28 Applied Imaging Corporation Photographic color couplers used as cytochemical contrast markers
DE19756782A1 (en) 1997-12-19 1999-06-24 Dade Behring Marburg Gmbh Detection and determination of solid phase associated factors
FR2777018B1 (en) 1998-04-02 2003-04-25 Bio Merieux NEW CHROMOGENEOUS SUBSTRATE AND METHOD OF USING THE SAME
AU7839500A (en) 1999-10-01 2001-05-10 Applied Imaging Corporation Simultaneous enzymatic detection of two analytes

Also Published As

Publication number Publication date
EP1332357A4 (en) 2004-07-14
ATE327509T1 (en) 2006-06-15
US6682903B2 (en) 2004-01-27
US20040115751A1 (en) 2004-06-17
WO2002033396A1 (en) 2002-04-25
US6936429B2 (en) 2005-08-30
EP1332357A1 (en) 2003-08-06
DE60119958D1 (en) 2006-06-29
DE60119958T2 (en) 2007-01-18
US20020123085A1 (en) 2002-09-05
EP1332357B1 (en) 2006-05-24

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