AU732321B2 - Method for identifying and inhibiting functional nucleic acid molecules in cells - Google Patents
Method for identifying and inhibiting functional nucleic acid molecules in cells Download PDFInfo
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- AU732321B2 AU732321B2 AU15323/99A AU1532399A AU732321B2 AU 732321 B2 AU732321 B2 AU 732321B2 AU 15323/99 A AU15323/99 A AU 15323/99A AU 1532399 A AU1532399 A AU 1532399A AU 732321 B2 AU732321 B2 AU 732321B2
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- 238000010369 molecular cloning Methods 0.000 description 1
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 1
- 229960000210 nalidixic acid Drugs 0.000 description 1
- 230000018791 negative regulation of catalytic activity Effects 0.000 description 1
- 229940051027 pasteurella multocida Drugs 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920000024 polymyxin B Polymers 0.000 description 1
- 229960005266 polymyxin b Drugs 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 210000000717 sertoli cell Anatomy 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 238000007860 single-cell PCR Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 208000006379 syphilis Diseases 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/126—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes involving RNAse P
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US976220 | 1997-11-21 | ||
| US08/976,220 US6013447A (en) | 1997-11-21 | 1997-11-21 | Random intracellular method for obtaining optimally active nucleic acid molecules |
| US7985198P | 1998-03-30 | 1998-03-30 | |
| US60/079851 | 1998-03-30 | ||
| PCT/US1998/024854 WO1999027135A2 (en) | 1997-11-21 | 1998-11-20 | Method for identifying and inhibiting functional nucleic acid molecules in cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU1532399A AU1532399A (en) | 1999-06-15 |
| AU732321B2 true AU732321B2 (en) | 2001-04-12 |
Family
ID=26762503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU15323/99A Ceased AU732321B2 (en) | 1997-11-21 | 1998-11-20 | Method for identifying and inhibiting functional nucleic acid molecules in cells |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1032707A2 (de) |
| JP (1) | JP2001524317A (de) |
| AU (1) | AU732321B2 (de) |
| CA (1) | CA2310510C (de) |
| WO (1) | WO1999027135A2 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1034308A4 (de) * | 1997-12-04 | 2002-04-03 | Smithkline Beecham Corp | Verfahren zur herstellung conditionell exprimierten mutanten zellen mit hilfe von expriemirten antisense sequenzen |
| AU4186600A (en) * | 1999-03-31 | 2000-10-16 | Rosetta Inpharmatics, Inc. | Essential genes of yeast as targets for antifungal agents, herbicides, insecticides and anti-proliferation drugs |
| GB9912965D0 (en) * | 1999-06-03 | 1999-08-04 | Oxford Biomedica Ltd | In vivo selection method |
| GB9929744D0 (en) * | 1999-12-17 | 2000-02-09 | Univ Nottingham | Modifying micro-organisms |
| WO2001048239A2 (de) * | 1999-12-23 | 2001-07-05 | Xantos Biomedicine Ag | Screening-verfahren für nukleinsäuren |
| AU2008202554C1 (en) * | 2001-02-23 | 2011-07-07 | Dsm Ip Assets B.V. | Novel genes encoding novel proteolytic enzymes |
| US20130244905A1 (en) * | 2010-07-06 | 2013-09-19 | Ed Grabczyk | Reporter for RNA Polymerase II Termination |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5168053A (en) * | 1989-03-24 | 1992-12-01 | Yale University | Cleavage of targeted RNA by RNAase P |
| EP0638121B1 (de) * | 1992-04-28 | 1996-07-17 | Yale University | Gezielte spaltung von rna mittels eukaryontischer rnase p und externe führungssequenz |
| JPH08507203A (ja) * | 1992-12-04 | 1996-08-06 | イノーバー ラボラトリーズ，インコーポレイテッド | 調節可能な核酸治療およびそれらの使用方法 |
| WO1996018733A2 (en) * | 1994-12-14 | 1996-06-20 | Innovir Laboratories, Inc. | Ribozyme-mediated inactivation of leukemia-associated rna |
| WO1997010360A1 (en) * | 1995-09-13 | 1997-03-20 | Chiron Corporation | Method and construct for screening for inhibitors of transcriptional activation |
| US5877162A (en) * | 1996-03-14 | 1999-03-02 | Innovir Laboratories, Inc. | Short external guide sequences |
| WO1998006837A1 (en) * | 1996-08-16 | 1998-02-19 | Yale University | Phenotypic conversion of drug-resistant bacteria to drug-sensitivity |
| AU5927598A (en) * | 1997-01-23 | 1998-08-18 | Immusol Incorporated | Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries |
-
1998
- 1998-11-20 EP EP98959542A patent/EP1032707A2/de not_active Withdrawn
- 1998-11-20 CA CA002310510A patent/CA2310510C/en not_active Expired - Fee Related
- 1998-11-20 AU AU15323/99A patent/AU732321B2/en not_active Ceased
- 1998-11-20 JP JP2000522276A patent/JP2001524317A/ja active Pending
- 1998-11-20 WO PCT/US1998/024854 patent/WO1999027135A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU1532399A (en) | 1999-06-15 |
| WO1999027135A2 (en) | 1999-06-03 |
| JP2001524317A (ja) | 2001-12-04 |
| CA2310510A1 (en) | 1999-06-03 |
| EP1032707A2 (de) | 2000-09-06 |
| CA2310510C (en) | 2007-04-17 |
| WO1999027135A3 (en) | 1999-07-29 |
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| FGA | Letters patent sealed or granted (standard patent) |