BE757338A - Fractionation of spermatozoa - Google Patents

Fractionation of spermatozoa

Info

Publication number
BE757338A
BE757338A BE757338DA BE757338A BE 757338 A BE757338 A BE 757338A BE 757338D A BE757338D A BE 757338DA BE 757338 A BE757338 A BE 757338A
Authority
BE
Belgium
Prior art keywords
fractionation
sperms
media
spermatozoa
fractionating
Prior art date
Application number
Other languages
French (fr)
Original Assignee
Shrimpton Wallace
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Publication date
Publication of BE757338A publication Critical patent/BE757338A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods
    • A61B17/42—Gynaecological or obstetrical instruments or methods
    • A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
    • A61B17/43—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for artificial insemination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Food Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Medical Informatics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Pathology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Spermatozoa are immobilised by cooling and introduced into a nutritive fractionating media. The fractionating media has a density gradient so that when the sperms are introduced into the middle of the column the denser X-chromosome contg. sperms descend and the less dense Y-chromosome contg. sperms rise. The fractionation is carried out under reduced pressure at -5 degrees to +2 degrees C and a pH of 6-8 in a media having a S.G. of 1.01-1.15 g./cm3 at 0 degree C. The appts. for the fractionation is claimed. The process allows one to choose the sex of the progeny of mammals for economic and social reasons.
BE757338D 1970-10-09 Fractionation of spermatozoa BE757338A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BE757338 1970-10-09

Publications (1)

Publication Number Publication Date
BE757338A true BE757338A (en) 1970-04-09

Family

ID=3857128

Family Applications (1)

Application Number Title Priority Date Filing Date
BE757338D BE757338A (en) 1970-10-09 Fractionation of spermatozoa

Country Status (1)

Country Link
BE (1) BE757338A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2462163A1 (en) * 1979-07-30 1981-02-13 Cassou Bertrand Dilution of sperm - by dialysis against diluent

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2462163A1 (en) * 1979-07-30 1981-02-13 Cassou Bertrand Dilution of sperm - by dialysis against diluent

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