BE900002A - PROCESS FOR THE PREPARATION OF THE TUBERCULINIC ANTIGEN L. - Google Patents

PROCESS FOR THE PREPARATION OF THE TUBERCULINIC ANTIGEN L.

Info

Publication number
BE900002A
BE900002A BE1/11049A BE1011049A BE900002A BE 900002 A BE900002 A BE 900002A BE 1/11049 A BE1/11049 A BE 1/11049A BE 1011049 A BE1011049 A BE 1011049A BE 900002 A BE900002 A BE 900002A
Authority
BE
Belgium
Prior art keywords
antigen
trisacryl
tuberculinic
preparation
crude
Prior art date
Application number
BE1/11049A
Other languages
French (fr)
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Priority to AT8484402716T priority Critical patent/ATE45500T1/en
Priority to EP19840402716 priority patent/EP0149395B1/en
Publication of BE900002A publication Critical patent/BE900002A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Pulmonology (AREA)
  • Communicable Diseases (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • Rheumatology (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'antigène L se caractérise par une activité plus forte sur des cobayes sensibilisés par du BCG vivant que sur des cobayes sensibilisés par du BCG inactivé. L'antigène L brut est obtenu par précipitation par de l'acide trichloracétique à 10 % du filtrat de culture préalablement clarifié et débarrassé d'une fraction étrangère à l'antigène L par de l'acide trichloracétique 2 %. Cet antigène L brut est purifié par chromatographie, successivement sur CM trisacryl M, DEAE trisacryl M, Ultrogel AcA 202 et trisacryl GF 05. C'est à chaque fois la fraction qui passera en fin de volume qui est active.The L antigen is characterized by a stronger activity on guinea pigs sensitized by living BCG than on guinea pigs sensitized by inactivated BCG. The crude L antigen is obtained by precipitation with trichloroacetic acid at 10% of the culture filtrate previously clarified and freed from a fraction foreign to the L antigen by 2% trichloroacetic acid. This crude L antigen is purified by chromatography, successively on CM trisacryl M, DEAE trisacryl M, Ultrogel AcA 202 and trisacryl GF 05. It is each time the fraction which will pass at the end of the volume which is active.

BE1/11049A 1983-12-28 1984-06-25 PROCESS FOR THE PREPARATION OF THE TUBERCULINIC ANTIGEN L. BE900002A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AT8484402716T ATE45500T1 (en) 1983-12-28 1984-12-26 PROCESS FOR THE PRODUCTION OF TUBERCULIN-L ANTIGENS.
EP19840402716 EP0149395B1 (en) 1983-12-28 1984-12-26 Method for the preparation of tuberculin-L antigen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8320912A FR2557457B1 (en) 1983-12-28 1983-12-28 PROCESS FOR THE PREPARATION OF THE TUBERCULINIC ANTIGEN L.

Publications (1)

Publication Number Publication Date
BE900002A true BE900002A (en) 1984-12-27

Family

ID=9295645

Family Applications (1)

Application Number Title Priority Date Filing Date
BE1/11049A BE900002A (en) 1983-12-28 1984-06-25 PROCESS FOR THE PREPARATION OF THE TUBERCULINIC ANTIGEN L.

Country Status (2)

Country Link
BE (1) BE900002A (en)
FR (1) FR2557457B1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123427A (en) * 1976-07-27 1978-10-31 Daniel Thomas M Method for the purification of mycobacterial protein antigens and resulting product

Also Published As

Publication number Publication date
FR2557457B1 (en) 1986-05-02
FR2557457A1 (en) 1985-07-05

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Legal Events

Date Code Title Description
RE Patent lapsed

Owner name: L' INSTITUT PASTEUR

Effective date: 19920630