BE902067A - Werkwijze en inrichting voor het meten van enzymconcentraties. - Google Patents

Werkwijze en inrichting voor het meten van enzymconcentraties. Download PDF

Info

Publication number: BE902067A
Authority: BE; Belgium
Prior art keywords: solution; electrode; graphite; aqueous solution; conductor
Prior art date: 1984-07-06

Application number

BE0/214744A

Other languages

English (en)

Dutch (nl)

Inventor

P Silverman

Original Assignee

Rohrback Tech

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-07-06

Filing date

1985-03-29

Publication date

1985-07-16

1985-03-29 Application filed by Rohrback Tech filed Critical Rohrback Tech

1985-07-16 Publication of BE902067A publication Critical patent/BE902067A/nl

Links

102000004190 Enzymes Human genes 0.000 title claims abstract description 20
108090000790 Enzymes Proteins 0.000 title claims abstract description 20
238000000034 method Methods 0.000 title claims description 27
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 31
239000010439 graphite Substances 0.000 claims abstract description 29
229910002804 graphite Inorganic materials 0.000 claims abstract description 29
241000894006 Bacteria Species 0.000 claims abstract description 12
230000002255 enzymatic effect Effects 0.000 claims description 19
239000003795 chemical substances by application Substances 0.000 claims description 17
238000005259 measurement Methods 0.000 claims description 11
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
239000001301 oxygen Substances 0.000 claims description 10
229910052760 oxygen Inorganic materials 0.000 claims description 10
239000000758 substrate Substances 0.000 claims description 10
ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 9
239000004020 conductor Substances 0.000 claims description 9
239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 8
235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 8
239000011248 coating agent Substances 0.000 claims description 7
238000000576 coating method Methods 0.000 claims description 7
239000000463 material Substances 0.000 claims description 6
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
230000005587 bubbling Effects 0.000 claims description 5
239000008103 glucose Substances 0.000 claims description 5
229910021645 metal ion Inorganic materials 0.000 claims description 5
229960000907 methylthioninium chloride Drugs 0.000 claims description 5
230000003647 oxidation Effects 0.000 claims description 5
238000007254 oxidation reaction Methods 0.000 claims description 5
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
238000010521 absorption reaction Methods 0.000 claims description 3
239000012535 impurity Substances 0.000 claims description 3
239000011261 inert gas Substances 0.000 claims description 3
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
230000003197 catalytic effect Effects 0.000 claims description 2
229910052742 iron Inorganic materials 0.000 claims description 2
230000007935 neutral effect Effects 0.000 claims description 2
239000000243 solution Substances 0.000 claims 13
239000007864 aqueous solution Substances 0.000 claims 9
239000008151 electrolyte solution Substances 0.000 claims 3
RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims 1
238000007654 immersion Methods 0.000 claims 1
239000002932 luster Substances 0.000 claims 1
238000004519 manufacturing process Methods 0.000 claims 1
230000002829 reductive effect Effects 0.000 abstract description 3
230000002596 correlated effect Effects 0.000 abstract 1
239000011810 insulating material Substances 0.000 abstract 1
XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 10
210000003739 neck Anatomy 0.000 description 8
238000012360 testing method Methods 0.000 description 8
238000006243 chemical reaction Methods 0.000 description 7
229910052786 argon Inorganic materials 0.000 description 5
CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 5
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
239000011521 glass Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
230000001580 bacterial effect Effects 0.000 description 3
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 3
229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
235000019796 monopotassium phosphate Nutrition 0.000 description 3
239000010970 precious metal Substances 0.000 description 3
238000013022 venting Methods 0.000 description 3
239000007836 KH2PO4 Substances 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
241001148470 aerobic bacillus Species 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
238000011088 calibration curve Methods 0.000 description 2
ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 2
235000019797 dipotassium phosphate Nutrition 0.000 description 2
229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
230000000694 effects Effects 0.000 description 2
239000003792 electrolyte Substances 0.000 description 2
239000003822 epoxy resin Substances 0.000 description 2
239000010410 layer Substances 0.000 description 2
239000007788 liquid Substances 0.000 description 2
238000005498 polishing Methods 0.000 description 2
229920000647 polyepoxide Polymers 0.000 description 2
239000001103 potassium chloride Substances 0.000 description 2
235000011164 potassium chloride Nutrition 0.000 description 2
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
JVXHQHGWBAHSSF-UHFFFAOYSA-L 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;hydron;iron(2+) Chemical compound [H+].[H+].[Fe+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O JVXHQHGWBAHSSF-UHFFFAOYSA-L 0.000 description 1
UOMQUZPKALKDCA-UHFFFAOYSA-K 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxymethyl)amino]acetate;iron(3+) Chemical compound [Fe+3].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UOMQUZPKALKDCA-UHFFFAOYSA-K 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
230000002411 adverse Effects 0.000 description 1
239000008272 agar Substances 0.000 description 1
238000003556 assay Methods 0.000 description 1
239000006172 buffering agent Substances 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
238000006555 catalytic reaction Methods 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
238000007922 dissolution test Methods 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
229960001484 edetic acid Drugs 0.000 description 1
239000007772 electrode material Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
229920006334 epoxy coating Polymers 0.000 description 1
239000012212 insulator Substances 0.000 description 1
230000002101 lytic effect Effects 0.000 description 1
239000003921 oil Substances 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
239000004814 polyurethane Substances 0.000 description 1
229920002635 polyurethane Polymers 0.000 description 1
239000011148 porous material Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
239000011253 protective coating Substances 0.000 description 1
230000001681 protective effect Effects 0.000 description 1
239000011241 protective layer Substances 0.000 description 1
238000010405 reoxidation reaction Methods 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
241000894007 species Species 0.000 description 1
238000004544 sputter deposition Methods 0.000 description 1
238000012546 transfer Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Sustainable Development (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Immunology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

BE0/214744A 1984-07-06 1985-03-29 Werkwijze en inrichting voor het meten van enzymconcentraties. BE902067A (nl)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US62851484A	1984-07-06	1984-07-06

Publications (1)

Publication Number	Publication Date
BE902067A true BE902067A (nl)	1985-07-16

Family

ID=24519209

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
BE0/214744A BE902067A (nl)	1984-07-06	1985-03-29	Werkwijze en inrichting voor het meten van enzymconcentraties.

Country Status (7)

Country	Link
JP (1)	JPS6125052A (de)
BE (1)	BE902067A (de)
DE (1)	DE3516230A1 (de)
FR (1)	FR2567268A1 (de)
GB (1)	GB2161274A (de)
LU (1)	LU85890A1 (de)
NL (1)	NL8500845A (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8523631D0 (en) *	1985-09-25	1985-10-30	Pena Ltd Paul De	Bioelectrochemical cell
JPH0244646U (de) *	1988-09-16	1990-03-27
FR2675260B1 (fr) *	1991-04-12	1994-10-21	Aix Marseille Univers Droit Ec	Procede et appareil pour le dosage electrochimique d'un corps dans une solution.
US6565738B1 (en)	1999-01-28	2003-05-20	Abbott Laboratories	Diagnostic test for the measurement of analyte in abiological fluid

1985
- 1985-03-22 NL NL8500845A patent/NL8500845A/nl not_active Application Discontinuation
- 1985-03-28 JP JP6496085A patent/JPS6125052A/ja active Pending
- 1985-03-29 BE BE0/214744A patent/BE902067A/nl not_active IP Right Cessation
- 1985-05-06 DE DE19853516230 patent/DE3516230A1/de not_active Withdrawn
- 1985-05-09 LU LU85890A patent/LU85890A1/de unknown
- 1985-05-15 FR FR8507425A patent/FR2567268A1/fr not_active Withdrawn
- 1985-06-06 GB GB8514285A patent/GB2161274A/en not_active Withdrawn

Also Published As

Publication number	Publication date
GB8514285D0 (en)	1985-07-10
FR2567268A1 (fr)	1986-01-10
GB2161274A (en)	1986-01-08
NL8500845A (nl)	1986-02-03
JPS6125052A (ja)	1986-02-03
DE3516230A1 (de)	1986-02-06
LU85890A1 (de)	1986-01-14

Legal Events

Date

Code

Title

Description

1987-09-30

RE

Patent lapsed

Owner name: ROHRBACK TECHNOLOGY CORP.

Effective date: 19870331

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