BE903626A - Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure. - Google Patents

Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.

Info

Publication number
BE903626A
BE903626A BE85215849A BE215849A BE903626A BE 903626 A BE903626 A BE 903626A BE 85215849 A BE85215849 A BE 85215849A BE 215849 A BE215849 A BE 215849A BE 903626 A BE903626 A BE 903626A
Authority
BE
Belgium
Prior art keywords
periplasmic space
yeast
recovery
localized
yeasts
Prior art date
Application number
BE85215849A
Other languages
English (en)
Original Assignee
Labofina Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Labofina Sa filed Critical Labofina Sa
Priority to BE85215849A priority Critical patent/BE903626A/fr
Publication of BE903626A publication Critical patent/BE903626A/fr
Priority to DE8686870159T priority patent/DE3684240D1/de
Priority to AT86870159T priority patent/ATE73490T1/de
Priority to EP86870159A priority patent/EP0222725B1/fr
Priority to ES198686870159T priority patent/ES2030000T3/es
Priority to JP61266772A priority patent/JP2636844B2/ja
Priority to CA000522859A priority patent/CA1339800C/fr
Priority to US07/607,844 priority patent/US5124256A/en
Priority to GR910402158T priority patent/GR3004042T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procéde pour assurer la libération en milieu aqueux de polypeptides produits par des levures et localisés au moins partiellement dans l'espace périplasmique de celles-ci; ce procédé est caractérisé en ce qu'il consiste à traiter ces levures en milieu aqueux avec un système comprenant d'une part un sel minéral soluble et neutre, et d'autre part un agent tensio-actif nonionique du type alkylphénol polyéthoxylé soluble dans l'eau et dont le HLB est compris entre 8 et 15.
BE85215849A 1985-11-13 1985-11-13 Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure. BE903626A (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BE85215849A BE903626A (fr) 1985-11-13 1985-11-13 Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.
DE8686870159T DE3684240D1 (de) 1985-11-13 1986-10-28 Verfahren zur rueckgewinnung von im periplasma von hefen lokalisierten polypeptiden.
AT86870159T ATE73490T1 (de) 1985-11-13 1986-10-28 Verfahren zur rueckgewinnung von im periplasma von hefen lokalisierten polypeptiden.
EP86870159A EP0222725B1 (fr) 1985-11-13 1986-10-28 Procédé de récupération de polypeptides localisés dans le périplasma de levures
ES198686870159T ES2030000T3 (es) 1985-11-13 1986-10-28 Procedimiento para recuperar polipeptidos localizados en el espacio periplasmico de levadura.
JP61266772A JP2636844B2 (ja) 1985-11-13 1986-11-11 酵母の外質隙中に局在するポリペプチドの回収法
CA000522859A CA1339800C (fr) 1985-11-13 1986-11-13 Procede d'extraction des polypeptides localises dans l'espace periplasmique d'une levure
US07/607,844 US5124256A (en) 1985-11-13 1990-11-05 Process for recovering polypeptides localized in the periplasmic space of yeast without breaking the cell wall by using an non-ionic detergent and a neutral salt
GR910402158T GR3004042T3 (fr) 1985-11-13 1992-03-12

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE903626 1985-11-13
BE85215849A BE903626A (fr) 1985-11-13 1985-11-13 Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.

Publications (1)

Publication Number Publication Date
BE903626A true BE903626A (fr) 1986-03-03

Family

ID=25654770

Family Applications (1)

Application Number Title Priority Date Filing Date
BE85215849A BE903626A (fr) 1985-11-13 1985-11-13 Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.

Country Status (8)

Country Link
EP (1) EP0222725B1 (fr)
JP (1) JP2636844B2 (fr)
AT (1) ATE73490T1 (fr)
BE (1) BE903626A (fr)
CA (1) CA1339800C (fr)
DE (1) DE3684240D1 (fr)
ES (1) ES2030000T3 (fr)
GR (1) GR3004042T3 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222725A3 (en) * 1985-11-13 1988-09-21 Labofina S.A. Process for recovering polypeptides localized in the periplasmic space of yeast
US5585257A (en) * 1988-09-26 1996-12-17 Fina Research S.A. Process for the production of human lysozyme

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7319021B2 (en) 2002-11-01 2008-01-15 Promega Corporation Cell lysis composition, methods of use, apparatus and kit
JP6778867B2 (ja) * 2015-08-07 2020-11-04 国立大学法人山口大学 ポリペプチド抽出方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63501121A (ja) * 1985-08-15 1988-04-28 アムジエン 酵母細胞からｂ型肝炎表面抗原を抽出するための細胞溶解法および緩衝液
BE903626A (fr) * 1985-11-13 1986-03-03 Labofina Sa Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222725A3 (en) * 1985-11-13 1988-09-21 Labofina S.A. Process for recovering polypeptides localized in the periplasmic space of yeast
US5585257A (en) * 1988-09-26 1996-12-17 Fina Research S.A. Process for the production of human lysozyme

Also Published As

Publication number Publication date
EP0222725A2 (fr) 1987-05-20
ATE73490T1 (de) 1992-03-15
ES2030000T3 (es) 1992-10-16
DE3684240D1 (de) 1992-04-16
EP0222725A3 (en) 1988-09-21
GR3004042T3 (fr) 1993-03-31
CA1339800C (fr) 1998-04-07
JP2636844B2 (ja) 1997-07-30
JPS62118896A (ja) 1987-05-30
EP0222725B1 (fr) 1992-03-11

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Legal Events

Date Code Title Description
RE Patent lapsed

Owner name: LABOFINA S.A.

Effective date: 19861130

RR Patent reestablished after lapse

Free format text: 871026 *LABOFINA S.A.

RE Patent lapsed

Owner name: S.A. FINA RESEARCH

Effective date: 19921130