BG104928A - Ефективно пречистване на аденовирус - Google Patents

Ефективно пречистване на аденовирус

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Publication number
BG104928A
BG104928A BG104928A BG10492800A BG104928A BG 104928 A BG104928 A BG 104928A BG 104928 A BG104928 A BG 104928A BG 10492800 A BG10492800 A BG 10492800A BG 104928 A BG104928 A BG 104928A
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BG
Bulgaria
Prior art keywords
adenovirus
chromatography resin
solution
eluting
applying
Prior art date
Application number
BG104928A
Other languages
English (en)
Inventor
Miguel Carrion
Marilyn Menger
Imre Kovesdi
Original Assignee
Genvec, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genvec, Inc. filed Critical Genvec, Inc.
Publication of BG104928A publication Critical patent/BG104928A/bg

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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/621—Detectors specially adapted therefor signal-to-noise ratio
    • G01N2030/625—Detectors specially adapted therefor signal-to-noise ratio by measuring reference material, e.g. carrier without sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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  • Water Treatment By Electricity Or Magnetism (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Dry Shavers And Clippers (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретението се отнася до метод за обогатяване на разтвор с аденовирус, при който смесен разтвор, включващ аденовирус и поне един нежелан тип биомолекула, се поставя върху анионообменна хроматографска смола, съдържаща свързваща група, избрана от диметиламинопропил, диметиламинобутил, диметиламиноизобутил и диметиламинопентил. В резултат аденовирусът елуира от хроматографската смола. Изобретението се отнася също и до метод за пречистване на аденовирус от инфектирани с аденовирус клетки, включващ лизиране на такива клетки, поставяне на лизата върху еднородна хроматографска смола, елуиране на аденовируса от нея и събиране на фракция, съдържащааденовирус с чистота, както на получения чрез трикратно пречистване в плътностен градиент от CsCl аденовирус. По метода е възможно точно количествено определяне на броя аденовирусни частици в разтвор от аденовирус, включващо поставяне върху анионообменна хроматографска смола и елуиране от нея на разтвор проба от аденовирус, сравняване на абсорбцията на разтвора проба и абсорбцията на стандартнияразтвор с аденовирус. Следва количествено определяне на броя вирусни частици в разтвора проба.
BG104928A 1998-04-22 2000-11-08 Ефективно пречистване на аденовирус BG104928A (bg)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8262898P 1998-04-22 1998-04-22
PCT/US1999/008843 WO1999054441A1 (en) 1998-04-22 1999-04-22 Efficient purification of adenovirus

Publications (1)

Publication Number Publication Date
BG104928A true BG104928A (bg) 2001-09-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BG104928A BG104928A (bg) 1998-04-22 2000-11-08 Ефективно пречистване на аденовирус

Country Status (20)

Country Link
US (3) US6383795B1 (bg)
EP (1) EP1073721B1 (bg)
JP (1) JP2002512361A (bg)
AT (1) ATE303434T1 (bg)
AU (1) AU756889B2 (bg)
BG (1) BG104928A (bg)
BR (1) BR9909789A (bg)
CA (1) CA2328462C (bg)
DE (1) DE69927013T2 (bg)
EA (1) EA200001096A1 (bg)
EE (1) EE200000605A (bg)
HU (1) HUP0102209A2 (bg)
IL (1) IL139040A0 (bg)
IS (1) IS5673A (bg)
MX (1) MXPA00010338A (bg)
NO (1) NO20005295L (bg)
PL (1) PL343630A1 (bg)
SK (1) SK15682000A3 (bg)
WO (1) WO1999054441A1 (bg)
ZA (1) ZA200005363B (bg)

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US20030203469A1 (en) 2003-10-30
DE69927013D1 (de) 2005-10-06
US6383795B1 (en) 2002-05-07
SK15682000A3 (sk) 2001-05-10
EA200001096A1 (ru) 2001-04-23
ATE303434T1 (de) 2005-09-15
NO20005295L (no) 2000-11-30
EP1073721B1 (en) 2005-08-31
EP1073721A1 (en) 2001-02-07
PL343630A1 (en) 2001-08-27
WO1999054441A8 (en) 2000-02-24
CA2328462C (en) 2010-11-09
JP2002512361A (ja) 2002-04-23
ZA200005363B (en) 2001-09-25
AU3662099A (en) 1999-11-08
AU756889B2 (en) 2003-01-23
NO20005295D0 (no) 2000-10-20
DE69927013T2 (de) 2006-06-29
BR9909789A (pt) 2000-12-26
CA2328462A1 (en) 1999-10-28
US7141406B2 (en) 2006-11-28
US20020034735A1 (en) 2002-03-21
MXPA00010338A (es) 2005-06-17
HUP0102209A2 (hu) 2001-09-28
IL139040A0 (en) 2001-11-25
EE200000605A (et) 2002-04-15
IS5673A (is) 2000-10-20
US6586226B2 (en) 2003-07-01
WO1999054441A1 (en) 1999-10-28

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