BG46006A3 - Method for preparing insecticide proteic substance - Google Patents
Method for preparing insecticide proteic substanceInfo
- Publication number
- BG46006A3 BG46006A3 BG078876A BG7887687A BG46006A3 BG 46006 A3 BG46006 A3 BG 46006A3 BG 078876 A BG078876 A BG 078876A BG 7887687 A BG7887687 A BG 7887687A BG 46006 A3 BG46006 A3 BG 46006A3
- Authority
- BG
- Bulgaria
- Prior art keywords
- dna
- insecticidal
- microorganism
- plasmid
- protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Методът намира приложение при растителната защита. С него се осигурява получаването на големи количества от крайния продукт инсектициден полипептид. Методът се състои в това, че клетки от в. Тнuringеnsis vаr. Кursтакi нdi се изолират и лизират, като плазмидите се отделят по известен начин. Плазмидният материал се пречиства и диализира. Приготвя се днк банка от плазмидната днк на микроорганизма, получената фрагментирана плазмидна днк се клонира в подходящ вектор. Извършва се подбор за наличие на протеина мgе 1. С днк фрагмента, който кодира инсектицидното протеиново вещество, включително отрязаните от него части, които са запазили инсектицидното си действие, се трансформира микроорганизъм. 15 претенцииThe method is used in plant protection. It ensures the production of large quantities of the final product insecticidal polypeptide. The method consists in isolating and lysing cells of C. thuringensis var. kurstaki ndi, and separating the plasmids in a known manner. The plasmid material is purified and dialyzed. A DNA bank is prepared from the plasmid DNA of the microorganism, the resulting fragmented plasmid DNA is cloned into a suitable vector. Selection is performed for the presence of the mge 1 protein. A microorganism is transformed with the DNA fragment encoding the insecticidal protein substance, including the parts cut from it that have retained their insecticidal effect. 15 claims
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8606441A GB2188049B (en) | 1986-03-15 | 1986-03-15 | Insecticidal proteinaceous substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BG46006A3 true BG46006A3 (en) | 1989-09-15 |
Family
ID=10594681
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG080405A BG46752A3 (en) | 1986-03-15 | 1987-03-03 | INSECTICIDE AND INSECT CONTROL METHOD |
| BG078876A BG46006A3 (en) | 1986-03-15 | 1987-03-13 | Method for preparing insecticide proteic substance |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG080405A BG46752A3 (en) | 1986-03-15 | 1987-03-03 | INSECTICIDE AND INSECT CONTROL METHOD |
Country Status (25)
| Country | Link |
|---|---|
| EP (1) | EP0238441B1 (en) |
| JP (1) | JPS62224295A (en) |
| KR (1) | KR900007640B1 (en) |
| AT (1) | ATE97163T1 (en) |
| AU (1) | AU608508B2 (en) |
| BG (2) | BG46752A3 (en) |
| BR (1) | BR8701162A (en) |
| DD (2) | DD259420A5 (en) |
| DE (1) | DE3788077D1 (en) |
| DK (1) | DK128687A (en) |
| EG (1) | EG18869A (en) |
| ES (1) | ES2059404T3 (en) |
| FI (1) | FI871070A7 (en) |
| GB (1) | GB2188049B (en) |
| HU (1) | HU204092B (en) |
| IE (1) | IE59456B1 (en) |
| IL (1) | IL81886A (en) |
| MA (1) | MA20900A1 (en) |
| NO (1) | NO871062L (en) |
| NZ (1) | NZ219611A (en) |
| PL (1) | PL154252B1 (en) |
| PT (1) | PT84474B (en) |
| TN (1) | TNSN87038A1 (en) |
| TR (1) | TR23521A (en) |
| ZA (1) | ZA871830B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0317511A3 (en) * | 1987-11-18 | 1991-10-16 | Ciba-Geigy Ag | Insecticidal cotton plant cells |
| CA2101610A1 (en) * | 1992-08-07 | 1994-02-08 | William D. Prevatt | Production of bacillus entomotoxins in methylotrophic yeast |
| GB9618083D0 (en) * | 1996-08-29 | 1996-10-09 | Mini Agriculture & Fisheries | Pesticidal agents |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57142907A (en) * | 1981-02-27 | 1982-09-03 | Shionogi & Co Ltd | Bacterium-originated insecticide and its preparation |
| JPH0795953B2 (en) * | 1981-04-27 | 1995-10-18 | ワシントン・リサ−チ・ファンデ−ション | Bacillus thuringiensis microcrystalline protein in Escherichia coli |
| FR2525630B1 (en) * | 1982-04-26 | 1985-07-05 | Pasteur Institut | DNA CONTAINING A SEQUENCE ENCODING A CRYSTAL PROTEIN OR A POLYPEPTIDE HAVING INSECTICIDE PROPERTIES, MICROORGANISMS TRANSFORMED BY SUCH DNA, COMPOSITIONS CONTAINING SAID CRYSTAL PROTEINS, POLYPEPTIDE OR MICROORGANISMS |
| GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
| CA1301094C (en) * | 1984-08-31 | 1992-05-19 | Helen Riaboff Whiteley | Bacillus thuringiensis crystal protein gene toxin segment |
| GB8425487D0 (en) * | 1984-10-09 | 1984-11-14 | Agricultural Genetics Co | Strain of bacillus thuringiensis |
| JPH074232B2 (en) * | 1984-12-10 | 1995-01-25 | モンサントコンパニー | Insertion of Bacillus thuringiensis crystal protein gene into a microorganism capable of forming a colony on a plant and its use |
| CA1341283C (en) * | 1985-03-28 | 2001-08-14 | Frank H. Gaertner | Biological pesticides and methods for their delivery and use |
| JPS62100292A (en) * | 1985-10-28 | 1987-05-09 | Sumitomo Chem Co Ltd | Insecticidal protein gene of bacillus thuringensis aizawai ipl strain |
| CA1341092C (en) | 1985-12-12 | 2000-09-05 | David L. Edwards | Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby |
-
1986
- 1986-03-15 GB GB8606441A patent/GB2188049B/en not_active Expired - Lifetime
-
1987
- 1987-03-03 BG BG080405A patent/BG46752A3/en unknown
- 1987-03-09 AT AT87810135T patent/ATE97163T1/en not_active IP Right Cessation
- 1987-03-09 EP EP87810135A patent/EP0238441B1/en not_active Expired - Lifetime
- 1987-03-09 DE DE87810135T patent/DE3788077D1/en not_active Expired - Lifetime
- 1987-03-09 ES ES87810135T patent/ES2059404T3/en not_active Expired - Lifetime
- 1987-03-11 FI FI871070A patent/FI871070A7/en not_active Application Discontinuation
- 1987-03-12 EG EG14887A patent/EG18869A/en active
- 1987-03-13 DD DD30077687A patent/DD259420A5/en unknown
- 1987-03-13 ZA ZA871830A patent/ZA871830B/en unknown
- 1987-03-13 HU HU871122A patent/HU204092B/en unknown
- 1987-03-13 BR BR8701162A patent/BR8701162A/en not_active Application Discontinuation
- 1987-03-13 DK DK128687A patent/DK128687A/en not_active Application Discontinuation
- 1987-03-13 MA MA21140A patent/MA20900A1/en unknown
- 1987-03-13 AU AU69992/87A patent/AU608508B2/en not_active Expired
- 1987-03-13 DD DD87316204A patent/DD297763A5/en not_active IP Right Cessation
- 1987-03-13 NO NO871062A patent/NO871062L/en unknown
- 1987-03-13 PL PL1987264628A patent/PL154252B1/en unknown
- 1987-03-13 NZ NZ219611A patent/NZ219611A/en unknown
- 1987-03-13 BG BG078876A patent/BG46006A3/en unknown
- 1987-03-13 IE IE66587A patent/IE59456B1/en not_active IP Right Cessation
- 1987-03-13 PT PT84474A patent/PT84474B/en not_active IP Right Cessation
- 1987-03-13 IL IL81886A patent/IL81886A/en not_active IP Right Cessation
- 1987-03-14 KR KR1019870002292A patent/KR900007640B1/en not_active Expired
- 1987-03-16 TN TNTNSN87038A patent/TNSN87038A1/en unknown
- 1987-03-16 JP JP62060879A patent/JPS62224295A/en active Pending
- 1987-03-16 TR TR163/87A patent/TR23521A/en unknown
Also Published As
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