BG46006A3 - Method for preparing insecticide proteic substance - Google Patents

Method for preparing insecticide proteic substance

Info

Publication number
BG46006A3
BG46006A3 BG078876A BG7887687A BG46006A3 BG 46006 A3 BG46006 A3 BG 46006A3 BG 078876 A BG078876 A BG 078876A BG 7887687 A BG7887687 A BG 7887687A BG 46006 A3 BG46006 A3 BG 46006A3
Authority
BG
Bulgaria
Prior art keywords
dna
insecticidal
microorganism
plasmid
protein
Prior art date
Application number
BG078876A
Other languages
Bulgarian (bg)
Inventor
Martin Geiser
Albert Hinnen
Jakob Brassel
Silvia Gruetzmacher
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of BG46006A3 publication Critical patent/BG46006A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Методът намира приложение при растителната защита. С него се осигурява получаването на големи количества от крайния продукт инсектициден полипептид. Методът се състои в това, че клетки от в. Тнuringеnsis vаr. Кursтакi нdi се изолират и лизират, като плазмидите се отделят по известен начин. Плазмидният материал се пречиства и диализира. Приготвя се днк банка от плазмидната днк на микроорганизма, получената фрагментирана плазмидна днк се клонира в подходящ вектор. Извършва се подбор за наличие на протеина мgе 1. С днк фрагмента, който кодира инсектицидното протеиново вещество, включително отрязаните от него части, които са запазили инсектицидното си действие, се трансформира микроорганизъм. 15 претенцииThe method is used in plant protection. It ensures the production of large quantities of the final product insecticidal polypeptide. The method consists in isolating and lysing cells of C. thuringensis var. kurstaki ndi, and separating the plasmids in a known manner. The plasmid material is purified and dialyzed. A DNA bank is prepared from the plasmid DNA of the microorganism, the resulting fragmented plasmid DNA is cloned into a suitable vector. Selection is performed for the presence of the mge 1 protein. A microorganism is transformed with the DNA fragment encoding the insecticidal protein substance, including the parts cut from it that have retained their insecticidal effect. 15 claims

BG078876A 1986-03-15 1987-03-13 Method for preparing insecticide proteic substance BG46006A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8606441A GB2188049B (en) 1986-03-15 1986-03-15 Insecticidal proteinaceous substance

Publications (1)

Publication Number Publication Date
BG46006A3 true BG46006A3 (en) 1989-09-15

Family

ID=10594681

Family Applications (2)

Application Number Title Priority Date Filing Date
BG080405A BG46752A3 (en) 1986-03-15 1987-03-03 INSECTICIDE AND INSECT CONTROL METHOD
BG078876A BG46006A3 (en) 1986-03-15 1987-03-13 Method for preparing insecticide proteic substance

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BG080405A BG46752A3 (en) 1986-03-15 1987-03-03 INSECTICIDE AND INSECT CONTROL METHOD

Country Status (25)

Country Link
EP (1) EP0238441B1 (en)
JP (1) JPS62224295A (en)
KR (1) KR900007640B1 (en)
AT (1) ATE97163T1 (en)
AU (1) AU608508B2 (en)
BG (2) BG46752A3 (en)
BR (1) BR8701162A (en)
DD (2) DD297763A5 (en)
DE (1) DE3788077D1 (en)
DK (1) DK128687A (en)
EG (1) EG18869A (en)
ES (1) ES2059404T3 (en)
FI (1) FI871070L (en)
GB (1) GB2188049B (en)
HU (1) HU204092B (en)
IE (1) IE59456B1 (en)
IL (1) IL81886A (en)
MA (1) MA20900A1 (en)
NO (1) NO871062L (en)
NZ (1) NZ219611A (en)
PL (1) PL154252B1 (en)
PT (1) PT84474B (en)
TN (1) TNSN87038A1 (en)
TR (1) TR23521A (en)
ZA (1) ZA871830B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317511A3 (en) * 1987-11-18 1991-10-16 Ciba-Geigy Ag Insecticidal cotton plant cells
CA2101610A1 (en) * 1992-08-07 1994-02-08 William D. Prevatt Production of bacillus entomotoxins in methylotrophic yeast
GB9618083D0 (en) * 1996-08-29 1996-10-09 Mini Agriculture & Fisheries Pesticidal agents

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57142907A (en) * 1981-02-27 1982-09-03 Shionogi & Co Ltd Bacterium-originated insecticide and its preparation
WO1982003872A1 (en) * 1981-04-27 1982-11-11 Washington Univ B.thuringiensis crystal protein in e.coli
FR2525630B1 (en) * 1982-04-26 1985-07-05 Pasteur Institut DNA CONTAINING A SEQUENCE ENCODING A CRYSTAL PROTEIN OR A POLYPEPTIDE HAVING INSECTICIDE PROPERTIES, MICROORGANISMS TRANSFORMED BY SUCH DNA, COMPOSITIONS CONTAINING SAID CRYSTAL PROTEINS, POLYPEPTIDE OR MICROORGANISMS
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
CA1301094C (en) * 1984-08-31 1992-05-19 Helen Riaboff Whiteley Bacillus thuringiensis crystal protein gene toxin segment
GB8425487D0 (en) * 1984-10-09 1984-11-14 Agricultural Genetics Co Strain of bacillus thuringiensis
DE3585275D1 (en) * 1984-12-10 1992-03-05 Monsanto Co USE OF THE BACILLUS THURINGIENSIS CRYSTAL PROTEIN IN PLANTS, COLONIZING MICROORGANISMS AND THEIR USE.
CA1341283C (en) * 1985-03-28 2001-08-14 Frank H. Gaertner Biological pesticides and methods for their delivery and use
JPS62100292A (en) * 1985-10-28 1987-05-09 Sumitomo Chem Co Ltd Insecticidal protein gene of bacillus thuringensis aizawai ipl strain
CA1341092C (en) 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Also Published As

Publication number Publication date
ES2059404T3 (en) 1994-11-16
JPS62224295A (en) 1987-10-02
IE870665L (en) 1987-09-15
TNSN87038A1 (en) 1990-01-01
KR870009016A (en) 1987-10-22
PL264628A1 (en) 1988-04-28
KR900007640B1 (en) 1990-10-17
FI871070A7 (en) 1987-09-16
DD297763A5 (en) 1992-01-23
ZA871830B (en) 1987-11-25
DD259420A5 (en) 1988-08-24
IL81886A (en) 1992-02-16
ATE97163T1 (en) 1993-11-15
IE59456B1 (en) 1994-02-23
NO871062L (en) 1987-09-16
AU608508B2 (en) 1991-04-11
NO871062D0 (en) 1987-03-13
IL81886A0 (en) 1987-10-20
HU204092B (en) 1991-11-28
DK128687D0 (en) 1987-03-13
PT84474B (en) 1989-11-10
HUT43628A (en) 1987-11-30
EG18869A (en) 1994-02-28
PL154252B1 (en) 1991-07-31
FI871070A0 (en) 1987-03-11
GB2188049A (en) 1987-09-23
PT84474A (en) 1987-04-01
NZ219611A (en) 1991-01-29
BR8701162A (en) 1988-01-12
BG46752A3 (en) 1990-02-15
FI871070L (en) 1987-09-16
AU6999287A (en) 1987-09-17
MA20900A1 (en) 1987-10-01
GB2188049B (en) 1990-09-05
DE3788077D1 (en) 1993-12-16
EP0238441B1 (en) 1993-11-10
DK128687A (en) 1987-09-16
EP0238441A2 (en) 1987-09-23
TR23521A (en) 1990-03-01
EP0238441A3 (en) 1988-08-10

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