BG52178B2 - Method for chemical synthesis and expression of the gene of human noncystein -interferon - Google Patents
Method for chemical synthesis and expression of the gene of human noncystein -interferonInfo
- Publication number
- BG52178B2 BG52178B2 BG75781A BG7578186A BG52178B2 BG 52178 B2 BG52178 B2 BG 52178B2 BG 75781 A BG75781 A BG 75781A BG 7578186 A BG7578186 A BG 7578186A BG 52178 B2 BG52178 B2 BG 52178B2
- Authority
- BG
- Bulgaria
- Prior art keywords
- gene
- synthetic
- noncystein
- interferon
- expression
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 229940079322 interferon Drugs 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 4
- 108020004705 Codon Proteins 0.000 abstract 2
- 239000004471 Glycine Substances 0.000 abstract 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract 1
- 108700005078 Synthetic Genes Proteins 0.000 abstract 1
- 108020005038 Terminator Codon Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 229930182817 methionine Natural products 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 210000003705 ribosome Anatomy 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method by which two-chain DNA issynthetized containing 144 sensible codons and one stopcodon, thefirst of the sensible codons being initating one, encoding theamino acid methionine, the second glycine and the remaining encodethe subsequent amino acids of the molecule of the mature humaninterferon situated after the glycine. The synthetic gene isflanked by two Hind III site by which it is connected to theexpress vector containing synthetic promotor and synthetic sitefor binding with the ribosomes after which industrialmicroorganism is transformed with the produced plasmid.5 claims
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BG75781A BG52178B2 (en) | 1986-07-18 | 1986-07-18 | Method for chemical synthesis and expression of the gene of human noncystein -interferon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BG75781A BG52178B2 (en) | 1986-07-18 | 1986-07-18 | Method for chemical synthesis and expression of the gene of human noncystein -interferon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BG52178B2 true BG52178B2 (en) | 1998-06-30 |
Family
ID=3917573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG75781A BG52178B2 (en) | 1986-07-18 | 1986-07-18 | Method for chemical synthesis and expression of the gene of human noncystein -interferon |
Country Status (1)
| Country | Link |
|---|---|
| BG (1) | BG52178B2 (en) |
-
1986
- 1986-07-18 BG BG75781A patent/BG52178B2/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0211299A3 (en) | Fusion proteins, method for their production and their use | |
| TW327194B (en) | Novel amyloid precursor proteins and methods of using same | |
| PL274669A1 (en) | Method of obtaining a gene able to code authentical human serum albumin | |
| ES8603574A1 (en) | Production of polypeptides with a human gamma-interferon activity. | |
| HUT46061A (en) | Dna sequences encoding hirudin-like protein and process for producing such proteins | |
| IL81018A (en) | Fusion proteins comprising small fragments of human interleukin-2 as ballast,process for its preparation and genes,vectors and transformed host containing said fragments and used in the process | |
| PH30889A (en) | Dna encoding equuine-gamma interferon and recombination production of equine ifn-polypeptides. | |
| WO1996017941A3 (en) | Production of c-terminal amidated peptides from recombinant protein constructs | |
| EP0395706A4 (en) | Production of gonorrheal pi proteins and vaccines | |
| DK0652903T3 (en) | New human recombinant gamma interferon | |
| DE3572843D1 (en) | Site-specific proteolysis by renin | |
| ATE5151T1 (en) | MICROBIOLOGICALLY PRODUCED POLYPEPTIDE WITH THE AMINO ACID SEQUENCE OF MONKEY PROINSULIN, DNA AND PLASMIDS CODING FOR THIS SEQUENCE, MICROORGANISMS CONTAINING SUCH GENETIC INFORMATION AND METHODS OF PRODUCTION THEREOF. | |
| FR2556365B1 (en) | INTERFERON-G CLONING AND EXPRESSION VECTORS, TRANSFORMED BACTERIA AND PROCESS FOR PREPARING INTERFERON-G | |
| ES2081801T3 (en) | EXPRESSION PLASMIDS. | |
| UA19039A (en) | Method for the production of erythropoietin | |
| EP0295285A1 (en) | Method for the production of bovine growth hormone using a synthetic gene. | |
| IE58994B1 (en) | Modification of the DNA sequence between the shine-dalgarn sequence and the start codon of the TRP operon to increase protein expression | |
| KR830007827A (en) | Method for preparing a novel recombinant plasmid | |
| CA1267086C (en) | Method of administering diuretic atrial peptides |