BG97669A - Method for the preparation of antigen from immune complexes for immune disgnaostics of enzootic bovine leucosis - Google Patents

Method for the preparation of antigen from immune complexes for immune disgnaostics of enzootic bovine leucosis

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Publication number
BG97669A
BG97669A BG97669A BG9766993A BG97669A BG 97669 A BG97669 A BG 97669A BG 97669 A BG97669 A BG 97669A BG 9766993 A BG9766993 A BG 9766993A BG 97669 A BG97669 A BG 97669A
Authority
BG
Bulgaria
Prior art keywords
complexes
antigen
immune
immune complexes
serum
Prior art date
Application number
BG97669A
Inventor
Kina K Noeva
Sijka I Zakharieva
Rajna K Vodenicharska
Original Assignee
Inst Obshta I Sravnitelna Pato
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Obshta I Sravnitelna Pato filed Critical Inst Obshta I Sravnitelna Pato
Priority to BG97669A priority Critical patent/BG97669A/en
Publication of BG97669A publication Critical patent/BG97669A/en

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Abstract

The method is immunochemical and is used for the production of antigen from immune complexes recirculating in the blood, as well as for the production of monoclonal antibodies by hybridome techniques. By it, serum from leucosis cows is fractionated by ion-echange chromatography on DEAE-cellulose at ionic strength of 0.02 and pH 7.6-8.0. Immune complexes are bound on the cellulose, and the serum immunoglobuline are eliminated by the eluate. The bound complexes are freed from the ion-exchange cellulose by eluation by 2.5-3.0 ml 2 M sodium chloride per ml initial serum. The ionic strength of the eluate is reduced to 0.15 by dialysis or desalination gelfiltration. In the protein solution produced the immune complexes are selectively settled at 3.5 polyethyleneglycol concentration in the mixture with mol. wt. 6000. The complexes settled and separated are washed once in 3.5% polyethyleneglycol and twice in acetone, are dispersed in 0.01 M Thris-hydrochloric acid buffer, pH 8.0 containing 0.1 - 0.02% sodium dodecylsulphate and 0.8 -1.0% 2-mercaptoethanol and are placed in water bath at 60°C for 30-40 min. The slightly opalescent solution containing the antigen is dialysed 24 hrs against PBS, pH 7.4 and is stored at -20°C.
BG97669A 1993-04-27 1993-04-27 Method for the preparation of antigen from immune complexes for immune disgnaostics of enzootic bovine leucosis BG97669A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BG97669A BG97669A (en) 1993-04-27 1993-04-27 Method for the preparation of antigen from immune complexes for immune disgnaostics of enzootic bovine leucosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BG97669A BG97669A (en) 1993-04-27 1993-04-27 Method for the preparation of antigen from immune complexes for immune disgnaostics of enzootic bovine leucosis

Publications (1)

Publication Number Publication Date
BG97669A true BG97669A (en) 1995-02-28

Family

ID=3925101

Family Applications (1)

Application Number Title Priority Date Filing Date
BG97669A BG97669A (en) 1993-04-27 1993-04-27 Method for the preparation of antigen from immune complexes for immune disgnaostics of enzootic bovine leucosis

Country Status (1)

Country Link
BG (1) BG97669A (en)

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