BR0014059A - Processes for producing a heterologous secretory protein, and for the secretory production of a mature transglutaminase, peptide specific for proline, polypeptide, and, nucleic acid molecule - Google Patents

Processes for producing a heterologous secretory protein, and for the secretory production of a mature transglutaminase, peptide specific for proline, polypeptide, and, nucleic acid molecule

Info

Publication number
BR0014059A
BR0014059A BR0014059-7A BR0014059A BR0014059A BR 0014059 A BR0014059 A BR 0014059A BR 0014059 A BR0014059 A BR 0014059A BR 0014059 A BR0014059 A BR 0014059A
Authority
BR
Brazil
Prior art keywords
transglutaminase
coryneform bacterium
foreign protein
secretory
secretory production
Prior art date
Application number
BR0014059-7A
Other languages
Portuguese (pt)
Other versions
BR0014059B1 (en
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Hiroshi Matsui
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BR0014059A publication Critical patent/BR0014059A/en
Publication of BR0014059B1 publication Critical patent/BR0014059B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production). An intended foreign protein, in particular, transglutaminase, is produced by using an expression construct wherein the gene sequence of the intended foreign protein containing the pro-structure part, in particular, pro-transglutaminase gene sequence, is ligated to the downstream of a sequence encoding the signal peptide region from a coryneform bacterium, introducing this expressional genetic construct into a coryneform bacterium, culturing the thus transformed coryneform bacterium, and treating the extracellularly released protein with a protease, etc. to cleave and eliminate the pro-part.
BRPI0014059-7A 1999-09-30 2000-09-29 process for producing transglutaminase. BR0014059B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP28009899 1999-09-30
JP2000194043 2000-06-28
PCT/JP2000/006780 WO2001023591A1 (en) 1999-09-30 2000-09-29 Process for producing transglutaminase

Publications (2)

Publication Number Publication Date
BR0014059A true BR0014059A (en) 2002-05-14
BR0014059B1 BR0014059B1 (en) 2013-02-05

Family

ID=26553616

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0014059-7A BR0014059B1 (en) 1999-09-30 2000-09-29 process for producing transglutaminase.

Country Status (12)

Country Link
US (2) US7723067B2 (en)
EP (1) EP1219713B1 (en)
JP (1) JP4320769B2 (en)
KR (1) KR100565030B1 (en)
CN (1) CN100497632C (en)
AT (1) ATE532870T1 (en)
AU (1) AU7449400A (en)
BR (1) BR0014059B1 (en)
CA (1) CA2391961C (en)
DK (1) DK1219713T3 (en)
ES (1) ES2372806T3 (en)
WO (1) WO2001023591A1 (en)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0113304A (en) 2000-08-17 2004-01-06 Ajinomoto Kk Method for designing and preparing a mutant transglutaminase, mutant mtg mutant transglutaminase, gene, recombinant DNA, microorganisms, method for producing a mutant mtg or mutant transglutaminase, and monocyclic mtg crystals
DE60234247D1 (en) 2001-03-30 2009-12-17 Ajinomoto Kk PROCESS FOR THE SECRETION AND PRODUCTION OF PROTEIN
US7485438B2 (en) 2002-03-01 2009-02-03 Szu-Yi Chou Method of producing polyvalent antigens
US7101695B2 (en) 2002-03-01 2006-09-05 Szu-Yi Chou Method of producing transglutaminase having broad substrate activity
JP4323147B2 (en) 2002-09-10 2009-09-02 天野エンザイム株式会社 Transglutaminase producing bacteria
WO2004029254A1 (en) * 2002-09-30 2004-04-08 Ajinomoto Co.,Inc. Method of producing stable isotope-labeled protein
CA2518049C (en) 2003-03-07 2013-05-07 Ajinomoto Co., Inc. Method of producing microbial transglutaminase
DK1748077T3 (en) 2004-04-20 2013-04-08 Ajinomoto Kk PROCEDURE FOR PRODUCING PROTEIN
CN101263225A (en) * 2005-08-18 2008-09-10 诺沃-诺迪斯克保健股份有限公司 Improved substrate specificity of transglutaminase
EP2054436A1 (en) * 2006-08-18 2009-05-06 Novo Nordisk Health Care AG Transglutaminase variants with improved specificity
JPWO2008099898A1 (en) 2007-02-15 2010-05-27 味の素株式会社 Disulfide bond-introduced transglutaminase
JP2010518842A (en) * 2007-02-22 2010-06-03 ノボ ノルディスク ヘルス ケア アーゲー Transglutaminase variants with improved specificity
EP2138049B1 (en) 2007-03-29 2014-06-25 Ajinomoto Co., Inc. Enzyme preparation for adhesion and method of producing adhesion-molded food product
KR20100085083A (en) 2007-11-19 2010-07-28 아지노모토 가부시키가이샤 Processed fiber product, and method for production thereof
EP2251421B1 (en) * 2008-02-13 2016-11-16 Amano Enzyme Inc. Stabilized transglutaminase and process for production thereof
TWI444146B (en) 2008-09-25 2014-07-11 Ajinomoto Kk Enzyme preparations for bound and formed foods and methods for producing bound and formed foods
WO2010101256A1 (en) * 2009-03-06 2010-09-10 味の素株式会社 Thermotolerant transglutaminase originating in actinomyces
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
CN103917656B (en) * 2011-10-25 2016-09-28 味之素株式会社 The secretion production method of protein
WO2013065772A1 (en) 2011-11-02 2013-05-10 味の素株式会社 Method for secreting and producing proteins
WO2013065869A1 (en) 2011-11-02 2013-05-10 Ajinomoto Co.,Inc. Method for secretory production of protein
CN102586167B (en) * 2012-03-01 2013-07-24 华南理工大学 Recombinant bacillus subtilis and method for producing transglutaminase by utilizing recombinant bacillus substilis
CN103060283B (en) * 2012-12-28 2014-05-28 天津科技大学 Polypeptide for inhibiting activity of bacillus subtilis transglutaminase (BTG) and its screening method and use
JP5673986B1 (en) * 2013-02-18 2015-02-18 味の素株式会社 Production method by protein precipitation
CN103146661A (en) * 2013-03-07 2013-06-12 哈尔滨工业大学 The Method of Improving the Transglutaminase Production of Streptomyces Mobara under MgCl2 Stress
RU2013119826A (en) 2013-04-29 2014-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") CORINEFORM BACTERIA AND METHOD FOR PRODUCING HETEROLOGICAL HYBRID PROTEINS
DK3287523T3 (en) 2015-04-24 2022-07-25 Ajinomoto Kk PROCEDURE FOR SECRETORY PRODUCTION OF PROTEIN
US11242545B2 (en) 2016-09-01 2022-02-08 Ningxia Eppen Biotech Co., Ltd Corynebacterium for producing L-lysine by fermentation
KR102335301B1 (en) 2016-10-21 2021-12-06 아지노모토 가부시키가이샤 secretory production method of protein
EP3530747A4 (en) 2016-10-21 2020-07-22 Ajinomoto Co., Inc. Protein secretory production method
JP7613833B2 (en) 2017-03-28 2025-01-15 味の素株式会社 Method for Producing RNA
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. A method for production of a protein having peptidylglycine alpha-hydroxylating monooxygenase activity
CN107574159B (en) * 2017-10-26 2020-05-08 江南大学 Mutant of glutamine transaminase expressed in active form
BR112020016770A2 (en) 2018-02-20 2020-12-15 Ajinomoto Co., Inc. METHOD FOR INDUCING RNA SILENCING AND TO PRODUCE A COMPOSITION TO INDUCE RNA SILENCING IN A TARGET ORGANISM, AND, COMPOSITION FOR INDUCING RNA SILENCING IN A TARGET ORGANISM
WO2019203368A1 (en) 2018-04-20 2019-10-24 味の素株式会社 Method for secretory production of protein
WO2021183680A1 (en) * 2020-03-13 2021-09-16 Curie Co. Inc. Transglutaminase variants
CN113528479B (en) * 2020-04-13 2023-01-24 中国科学院微生物研究所 Efficient preparation method of transglutaminase and special engineering bacteria thereof
KR20230023751A (en) 2020-06-11 2023-02-17 아지노모토 가부시키가이샤 recipe for protein
CN111944778B (en) * 2020-08-14 2022-06-21 安徽医学高等专科学校 Glutamine transaminase mutant and encoding gene and application thereof
KR20240032941A (en) 2021-07-07 2024-03-12 아지노모토 가부시키가이샤 Secretion production method for proteins containing non-natural amino acids
WO2023200008A1 (en) 2022-04-15 2023-10-19 味の素株式会社 Method for producing cultured meat
US20250304927A1 (en) * 2022-05-20 2025-10-02 Curie Co. Inc. Pro-peptide variants for modulating activity of transglutaminases
KR20250151452A (en) 2023-02-16 2025-10-21 아지노모토 가부시키가이샤 Secretory production of proteins

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8800352A1 (en) * 1985-04-05 1987-10-16 Sankyo Co Human pancreatic elastase.
RU2091490C1 (en) 1985-10-25 1997-09-27 Зимодженетикс, Инк. Method of preparing the heterologic polypeptide in eucaryotic microorganisms
US4965194A (en) * 1986-08-21 1990-10-23 Toyo Boseki Kabushiki Kaisha Pyruvate oxidase and an analytical method using the same
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
JPH05244947A (en) * 1992-03-04 1993-09-24 Mercian Corp New post-proline endopeptidase and its production
JPH0736754B2 (en) 1993-07-16 1995-04-26 三共株式会社 Human / Pancreatic elastase IIIB
JPH09316095A (en) * 1996-05-27 1997-12-09 Mitsubishi Chem Corp Novel signal peptide
JP3711658B2 (en) * 1996-10-07 2005-11-02 味の素株式会社 A novel cell surface protein derived from Corynebacterium ammoniagenes
JP3305609B2 (en) 1997-03-06 2002-07-24 ダイハツ工業株式会社 Support structure for vehicle spare tires
JPH10244947A (en) 1997-03-06 1998-09-14 Toyoda Gosei Co Ltd Steering wheel core metal

Also Published As

Publication number Publication date
ATE532870T1 (en) 2011-11-15
CA2391961A1 (en) 2001-04-05
US20100297729A1 (en) 2010-11-25
KR100565030B1 (en) 2006-03-30
EP1219713A1 (en) 2002-07-03
EP1219713A4 (en) 2005-02-02
WO2001023591A1 (en) 2001-04-05
CN100497632C (en) 2009-06-10
JP4320769B2 (en) 2009-08-26
CA2391961C (en) 2008-12-02
US20030082746A1 (en) 2003-05-01
AU7449400A (en) 2001-04-30
EP1219713B1 (en) 2011-11-09
US7972829B2 (en) 2011-07-05
DK1219713T3 (en) 2011-12-12
US7723067B2 (en) 2010-05-25
BR0014059B1 (en) 2013-02-05
KR20020038787A (en) 2002-05-23
ES2372806T3 (en) 2012-01-26
CN1377413A (en) 2002-10-30

Similar Documents

Publication Publication Date Title
BR0014059B1 (en) process for producing transglutaminase.
DE60234247D1 (en) PROCESS FOR THE SECRETION AND PRODUCTION OF PROTEIN
US20080153132A1 (en) Genetic recombinant ecarin and process for preparing the same
AU7149691A (en) Process for enzymatical cleavage of recombinant proteins by means of iga proteases
EP0889133B1 (en) Process for producing microbial transglutaminase
DE69434292D1 (en) FOR ADSEVERIN ENCODING GEN
JPS6420094A (en) Production and purification of protein fused to binding protein
ATE480624T1 (en) YAPSIN DEFICIENCY MUTANT STRAIN OF HANSENULA POLYMORPHA AND METHOD FOR PRODUCING RECOMBINANT PROTEINS THEREFROM
EP0127658A1 (en) A process for preparing ripe proteins from fusion proteins, synthetized in pro- or eukaryotic cells.
DK0776971T3 (en) Hyperthermostable protease gene
DE60328802D1 (en) PROCESS FOR THE PREPARATION OF MAMMALS TRYPSINS
DE3572843D1 (en) Site-specific proteolysis by renin
BR9000445A (en) PROCESS FOR THE PRODUCTION OF GLUCAGON, DNA SEQUENCE ENCODING GLUCAGON, EXPRESSION VECTOR, TRANSFORMANT AND PURIFIED GLUCAGON
EP0352387B1 (en) Method for preparing a hirudin
USRE38981E1 (en) DNA sequence coding for protein C
AU2008202374B2 (en) Genetically modified ecarin and process for producing the same
DE60123077D1 (en) GENE FOR PROTEINS THAT ARE CAPABLE OF REGENERATING LUZIFERIN, ENCODING, RECOMBINANT DNA, AND METHOD FOR PRODUCING PROTEINS THAT ARE CAPABLE OF REGENERATING LUCIFERINE
WO2005103264A1 (en) Process for the preparation of recombinant amediplase

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]

Free format text: O DEPOSITANTE DEVERA APRESENTAR A PETICAO DEVIDA PARA O CUMPRIMENTO DESTA EXIGENCIA EM ATE 60 (SESSENTA) DIAS, CONTADOS A PARTIR DA DATA DE PUBLICACAO NA RPI, DE ACORDO COM O ART. 34 (II) DA LPI PARA QUE SE DE INICIO AO EXAME TECNICO DO PEDIDO.

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 13 E 10 (IX) DA LPI.

B09S Decision of refusal: publication cancelled [chapter 9.2.2 patent gazette]

Free format text: REFERENTE A RPI 2096 DE 09/03/2011.

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09X Republication of the decision to grant [chapter 9.1.3 patent gazette]

Free format text: O PRESENTE PEDIDO TEVE UM PARECER DE DEFERIMENTO NOTIFICADO NA RPI NO 2151 DE 27/03/2012, TENDO SIDO CONSTATADO QUE ESTA NOTIFICACAO FOI EFETUADA COM INCORRECOES (O PARECER DE DEFERIMENTO FOI PUBLICADO COM O TITULO INICIAL E NAO COM O TITULO FINAL MODIFICADO ATRAVES DA PETICAO NO 20100096356 DE 14/10/2010). ASSIM SENDO, REPUBLICO A REFERIDA PUBLICACAO.

B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 05/02/2013, OBSERVADAS AS CONDICOES LEGAIS.