BR0314841A - Vetor de dna, cultura, plantas angiospérmicas transplastÈmicas, progênie das mesmas e purificação e utilização da proteìna ou proteìnas que produzem as células das plantas - Google Patents

Vetor de dna, cultura, plantas angiospérmicas transplastÈmicas, progênie das mesmas e purificação e utilização da proteìna ou proteìnas que produzem as células das plantas

Info

Publication number
BR0314841A
BR0314841A BR0314841-6A BR0314841A BR0314841A BR 0314841 A BR0314841 A BR 0314841A BR 0314841 A BR0314841 A BR 0314841A BR 0314841 A BR0314841 A BR 0314841A
Authority
BR
Brazil
Prior art keywords
dna vector
transplastic
progeny
proteins
culture
Prior art date
Application number
BR0314841-6A
Other languages
English (en)
Inventor
Guillermo Selman-Housen Sosa
Eduardo Aguiar Cabeza
Annery Del Carmen Gon Quintero
Osmany Ramos Gonzalez
Original Assignee
Ct Ingenieria Genetica Y Biote
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Y Biote filed Critical Ct Ingenieria Genetica Y Biote
Publication of BR0314841A publication Critical patent/BR0314841A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"VETOR DE DNA, CULTURA, PLANTAS ANGIOSPéRMICAS TRANSPLASTÈMICAS, PROGENIE DAS MESMAS E PURIFICAçãO E UTILIZAçãO DA PROTEìNA OU PROTEìNAS QUE PRODUZEM AS CELULAS DAS PLANTAS". A invenção refere-se a um vetor de DNA que permite a inserção estável e a expressão de genes heterólogos nos genomas de plastídios de células de qualquer planta angiospérmica. Neste vetor, os genes a serem introduzidos podem ser inseridos em múltiplos sitios de clonagem localizados em uma região intergênica artificial que se obtém combinando os óperons atpB e rbeL pertencentes a plantas de classes diferentes (dicotiledóneas ou monocotiledóneas). A cassete de expressão é integrada no genoma mediante recombinação entre as seq³ências de borda atpB e rbcL do vetor, com as correspondentes regiões homólogas do genoma plastidico; sendo possível expressar mais de um gene de interesse a partir do promotor rbcL adjacente à região de borda que codifica para o gene atpB.
BR0314841-6A 2002-09-27 2003-09-15 Vetor de dna, cultura, plantas angiospérmicas transplastÈmicas, progênie das mesmas e purificação e utilização da proteìna ou proteìnas que produzem as células das plantas BR0314841A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20020208 2002-09-27
PCT/CU2003/000009 WO2004029256A2 (es) 2002-09-27 2003-09-15 Vector para la producción de plantas angiospermas transplastómicas

Publications (1)

Publication Number Publication Date
BR0314841A true BR0314841A (pt) 2005-08-09

Family

ID=32034750

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0314841-6A BR0314841A (pt) 2002-09-27 2003-09-15 Vetor de dna, cultura, plantas angiospérmicas transplastÈmicas, progênie das mesmas e purificação e utilização da proteìna ou proteìnas que produzem as células das plantas

Country Status (12)

Country Link
US (1) US20060026704A1 (pt)
EP (1) EP1557468A2 (pt)
JP (1) JP2006500037A (pt)
KR (1) KR20050054928A (pt)
CN (1) CN1685047A (pt)
AR (1) AR041375A1 (pt)
AU (1) AU2003266096A1 (pt)
BR (1) BR0314841A (pt)
CA (1) CA2496913A1 (pt)
MX (1) MXPA05003185A (pt)
WO (1) WO2004029256A2 (pt)
ZA (1) ZA200501740B (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
EP2573188A2 (en) 2005-03-02 2013-03-27 Metanomics GmbH Process for the production of fine chemicals
JP2006246744A (ja) * 2005-03-09 2006-09-21 Research Organization Of Information & Systems 遺伝子導入方法、ジーンターゲッティング方法及びトランスジェニック植物の製造方法
KR100740694B1 (ko) * 2006-02-09 2007-07-18 한국생명공학연구원 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
GB0604938D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Proteins, nucleic acids and medicaments
GB0604964D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Protein folding
GB0604966D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Medicaments and proteins
GB0617816D0 (en) * 2006-09-11 2006-10-18 Renovo Ltd Nucleic acids and methods of protein expression
EP2118289B1 (en) 2007-03-09 2013-12-11 Monsanto Technology, LLC Preparation and use of plant embryo explants for transformation
US20110037083A1 (en) * 2009-01-14 2011-02-17 Alex Chi Keung Chan Led package with contrasting face
CN102618566B (zh) * 2011-01-31 2014-01-08 中国科学院海洋研究所 一种构建亚心型扁藻叶绿体表达系统的方法
CN108192897A (zh) * 2018-02-11 2018-06-22 云南省烟草农业科学研究院 一种烟草rbcl基因片段及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
DE69632403T2 (de) * 1995-08-10 2005-05-19 Rutgers University Zellkern-codiertes transkriptionssystem plastiden höherer pflanzen
WO1998055595A1 (en) * 1997-06-03 1998-12-10 Rutgers, The State University Of New Jersey Plastid promoters for transgene expression in the plastids of higher plants

Also Published As

Publication number Publication date
US20060026704A1 (en) 2006-02-02
EP1557468A2 (en) 2005-07-27
MXPA05003185A (es) 2005-06-08
JP2006500037A (ja) 2006-01-05
CN1685047A (zh) 2005-10-19
AR041375A1 (es) 2005-05-11
WO2004029256A2 (es) 2004-04-08
AU2003266096A1 (en) 2004-04-19
KR20050054928A (ko) 2005-06-10
ZA200501740B (en) 2005-09-06
CA2496913A1 (en) 2004-04-08
WO2004029256A3 (es) 2004-04-29

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE 4A., 5A., 6A. E 7A. ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2069 DE 31/08/2010.