BR112012000242A2 - método para cultivo em batelada de células eucariontes, método para o cultivo de perfusão de células eucariontes e aparelhos para cultura de células eucariontes - Google Patents

método para cultivo em batelada de células eucariontes, método para o cultivo de perfusão de células eucariontes e aparelhos para cultura de células eucariontes

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Publication number
BR112012000242A2
BR112012000242A2 BR112012000242A BR112012000242A BR112012000242A2 BR 112012000242 A2 BR112012000242 A2 BR 112012000242A2 BR 112012000242 A BR112012000242 A BR 112012000242A BR 112012000242 A BR112012000242 A BR 112012000242A BR 112012000242 A2 BR112012000242 A2 BR 112012000242A2
Authority
BR
Brazil
Prior art keywords
eukaryotic cell
culture method
culture
perfusion
batch
Prior art date
Application number
BR112012000242A
Other languages
English (en)
Other versions
BR112012000242B1 (pt
Inventor
Dinesh Baskar
Inn H Yuk
Jenny Hsiung
Woon-Lam Susan Leung
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BR112012000242A2 publication Critical patent/BR112012000242A2/pt
Publication of BR112012000242B1 publication Critical patent/BR112012000242B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112012000242-2A 2009-07-06 2010-07-06 Métodos para cultivo em batelada de células eucariontes BR112012000242B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22331309P 2009-07-06 2009-07-06
US61/223,313 2009-07-06
PCT/US2010/041082 WO2011005773A2 (en) 2009-07-06 2010-07-06 Method of culturing eukaryotic cells

Publications (2)

Publication Number Publication Date
BR112012000242A2 true BR112012000242A2 (pt) 2015-09-08
BR112012000242B1 BR112012000242B1 (pt) 2019-07-09

Family

ID=43429807

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012000242-2A BR112012000242B1 (pt) 2009-07-06 2010-07-06 Métodos para cultivo em batelada de células eucariontes

Country Status (14)

Country Link
US (4) US20120329151A1 (pt)
EP (2) EP3643773A1 (pt)
JP (2) JP2012531930A (pt)
KR (1) KR101838302B1 (pt)
CN (2) CN102482633A (pt)
AU (4) AU2010270726A1 (pt)
BR (1) BR112012000242B1 (pt)
CA (2) CA2766902C (pt)
ES (1) ES2752874T3 (pt)
IL (3) IL217223A0 (pt)
MX (3) MX382080B (pt)
PL (1) PL2451936T3 (pt)
SG (1) SG177555A1 (pt)
WO (1) WO2011005773A2 (pt)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007294731B2 (en) 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
BRPI0920572A8 (pt) 2008-10-20 2015-10-27 Abbott Lab Inativação viral durante a purificação dos anticorpos
NZ592095A (en) 2008-10-20 2013-01-25 Abbott Lab Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography
BR112012000242B1 (pt) 2009-07-06 2019-07-09 Genentech Inc Métodos para cultivo em batelada de células eucariontes
US9428724B2 (en) 2011-02-24 2016-08-30 Ge Healthcare Bio-Sciences Ab Bioreactor with feed and harvest flow through filter assembly
US9376654B2 (en) * 2011-03-03 2016-06-28 Meissner Filtration Products, Inc. Biocontainer
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN202786257U (zh) 2011-08-31 2013-03-13 通用电气健康护理生物科学股份公司 用于生物反应器的排气过滤装置
SG11201402997QA (en) * 2011-12-09 2014-07-30 Atmi Packaging Inc Filtration apparatus for continuous perfusion
US9228166B2 (en) * 2011-12-20 2016-01-05 Pall Corporation Rockable biocontainer
EP2639294A1 (fr) * 2012-03-15 2013-09-18 CellProthera Automate et procédé automatisé de culture cellulaire
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
JP2013237644A (ja) * 2012-05-16 2013-11-28 Mitsubishi Gas Chemical Co Inc 細胞活性化炭酸水
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
CN107267385B (zh) * 2012-10-26 2021-03-12 麻省理工学院 小容积反应器中二氧化碳水平和pH的控制
EP4036208A1 (en) 2012-10-26 2022-08-03 Massachusetts Institute Of Technology Humidity control in chemical reactors
HK1207960A1 (en) 2013-03-12 2016-02-19 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
HUE056073T2 (hu) * 2013-03-15 2022-01-28 Genzyme Corp Magas denzitású sejtbanki tárolási eljárások
CA3173080A1 (en) 2013-08-23 2015-02-26 Sanofi Small volume bioreactors with substantially constant working volumes and associated systems and methods
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
TWI743024B (zh) 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI707949B (zh) * 2014-06-09 2020-10-21 美商健臻公司 種子罐培養法（ｓｅｅｄ ｔｒａｉｎ ｐｒｏｃｅｓｓｅｓ）及其用途
GB201416362D0 (en) * 2014-07-25 2014-10-29 Ge Healthcare Bio Sciences Ab Method and system for suspension culture
US20160100570A1 (en) * 2014-09-18 2016-04-14 Genzyme Corporation Ultra-high density cell banking methods
US10617070B2 (en) * 2014-10-06 2020-04-14 Life Technologies Corporation Methods and systems for culturing microbial and cellular seed cultures
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
KR102427457B1 (ko) * 2015-03-24 2022-08-01 메디칸(주) 연속 세포 배양 증식장치 및 방법
GB201509202D0 (en) 2015-05-28 2015-07-15 Ge Healthcare Bio Sciences Ab Semi-static cell culture
CN105176815B (zh) * 2015-10-20 2017-03-22 哈尔滨工业大学 一种用于人工皮肤组织培养的气液界面灌注式生物反应器及其制备方法和使用方法
JP6893208B2 (ja) 2015-10-30 2021-06-23 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｐｈ測定装置の較正ずれの特定
US11136542B2 (en) 2016-02-23 2021-10-05 Corning Incorporated Perfusion bioreactor and method for using same to perform a continuous cell culture
CN105802851B (zh) * 2016-03-25 2018-06-19 苏州诺普再生医学有限公司 一种3d打印皮肤的动态倾斜旋转生物反应器
CN105820948B (zh) * 2016-03-31 2018-06-01 上海高机生物工程有限公司 一种用于生物反应袋的旋摆平台
WO2018010929A1 (en) * 2016-07-12 2018-01-18 General Electric Company Microfluidic device for cell culture monitoring
US10913063B2 (en) 2016-07-12 2021-02-09 EMULATE, Inc. Removing bubbles in a microfluidic device
BR112019004662A2 (pt) * 2016-09-12 2019-05-28 Juno Therapeutics Inc conjuntos de sacos de biorreator de perfusão
WO2018052991A1 (en) * 2016-09-14 2018-03-22 Vbc Holdings Llc Systems, apparatus and methods for controlling a movement of a cell culture to optimize cell growth
CA3044251A1 (en) * 2016-11-18 2018-05-24 New York Stem Cell Foundation, Inc. Microfluidic system and method of use thereof
US20200063086A1 (en) * 2016-12-21 2020-02-27 Hoffmann-La Roche Inc. Growth control of eukaryotic cells
CN110139923A (zh) * 2016-12-29 2019-08-16 通用电气公司 细胞培养生物反应器的故障恢复
KR101895195B1 (ko) * 2017-03-17 2018-09-05 인제대학교 산학협력단 세포 체외 증식을 위한 바이오리액터
US11609120B2 (en) 2017-10-06 2023-03-21 Lonza Ltd Automated control of cell culture using Raman spectroscopy
EP4039786A1 (en) 2017-10-16 2022-08-10 Regeneron Pharmaceuticals, Inc. Perfusion bioreactor and related methods of use
JPWO2019131626A1 (ja) * 2017-12-28 2020-12-10 オリンパス株式会社 細胞培養制御方法、細胞培養制御装置、細胞培養装置および細胞培養システム
WO2020206121A2 (en) * 2019-04-03 2020-10-08 Flaskworks, Llc Cell culture systems and uses thereof
US11708555B2 (en) 2019-05-30 2023-07-25 Global Life Sciences Solutions Usa Llc System, method and apparatus for draining a bioreactor vessel
US20220333049A1 (en) * 2019-09-06 2022-10-20 Zhejiang Jinyishengshi Bioengineering Co.,Ltd Bioreactor system and application thereof
JP2022551049A (ja) * 2019-10-11 2022-12-07 サイティバ・スウェーデン・アクチボラグ バイオリアクタ装置およびその動作方法
EP3985089A1 (en) * 2020-10-15 2022-04-20 The Automation Partnership (Cambridge) Ltd. Bioreactor including probe for electrical or electromagnetic measurements
KR20230145343A (ko) * 2021-02-12 2023-10-17 바이엘 헬쓰케어 엘엘씨 세포 배양 배지의 pH를 조정하는 방법
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same
EP4491703B1 (en) * 2023-07-12 2026-01-28 Imec VZW A sensor head and a method for manufacturing a sensor head
WO2025205600A1 (ja) * 2024-03-25 2025-10-02 三菱ケミカル株式会社 細胞培養方法及び細胞培養装置

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3941662A (en) * 1971-06-09 1976-03-02 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Apparatus for culturing cells
JPS5238082A (en) 1975-09-13 1977-03-24 Heraeus Gmbh W C Cultivating container for microorganism * bacterium and tissue
JPS60141286A (ja) * 1983-12-28 1985-07-26 Ajinomoto Co Inc 動物細胞の培養方法および装置
US4680267A (en) * 1985-03-01 1987-07-14 New Brunswick Scientific Company, Inc. Fermentor control system
DE3515753C2 (de) * 1985-05-02 1987-03-05 Hans 4156 Willich Preisendanz Verfahren zur Bestimmung der Wirkung von Chemotherapeutika auf das Wachstum von Mikroorganismen und/oder Zellen und Verwendung einer Vorrichtung mit mehreren Kammern zu seiner Durchführung
JPH0310679A (ja) * 1989-06-09 1991-01-18 Shimadzu Corp 細胞培養バッグ
EP0567738A3 (en) * 1992-05-01 1995-09-06 American Cyanamid Co Controlling perfusion rates in continuous bioreactor culture of animal cells
US6190913B1 (en) * 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US8026096B1 (en) 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
US6544788B2 (en) * 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
DK1773976T4 (da) * 2004-06-04 2020-02-10 Global Life Sciences Solutions Usa Llc Engangsbioreaktorsystemer og -fremgangsmåder
US7195394B2 (en) * 2004-07-19 2007-03-27 Vijay Singh Method for resonant wave mixing in closed containers
US20060196501A1 (en) * 2005-03-02 2006-09-07 Hynetics Llc Systems and methods for mixing and sparging solutions and/or suspensions
US7745209B2 (en) 2005-07-26 2010-06-29 Corning Incorporated Multilayered cell culture apparatus
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
US8215238B2 (en) 2007-10-10 2012-07-10 The Texas A&M University System Guideway coupling system
BR112012000242B1 (pt) 2009-07-06 2019-07-09 Genentech Inc Métodos para cultivo em batelada de células eucariontes

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MX382080B (es) 2025-03-13
EP2451936B1 (en) 2019-08-28
WO2011005773A3 (en) 2012-01-26
JP6358999B2 (ja) 2018-07-18
CA2766902C (en) 2021-07-06
EP3643773A1 (en) 2020-04-29
JP2012531930A (ja) 2012-12-13
AU2021200110A1 (en) 2021-03-18
US20120329151A1 (en) 2012-12-27
IL267629A (en) 2019-08-29
CN107043703A (zh) 2017-08-15
AU2018250474A1 (en) 2018-11-15
MX367489B (es) 2019-08-23
IL242092B (en) 2019-07-31
US10190084B2 (en) 2019-01-29
EP2451936A2 (en) 2012-05-16
BR112012000242B1 (pt) 2019-07-09
KR101838302B1 (ko) 2018-03-13
AU2016202997A1 (en) 2016-05-26
US11939559B2 (en) 2024-03-26
SG177555A1 (en) 2012-02-28
WO2011005773A2 (en) 2011-01-13
CN107043703B (zh) 2020-03-27
CA3118479A1 (en) 2011-01-13
CN102482633A (zh) 2012-05-30
JP2016063816A (ja) 2016-04-28
KR20120047930A (ko) 2012-05-14
PL2451936T3 (pl) 2020-02-28
IL217223A0 (en) 2012-02-29
US20210130759A1 (en) 2021-05-06
MX2012000354A (es) 2012-02-01
US20190233790A1 (en) 2019-08-01
AU2010270726A1 (en) 2012-02-02
AU2018250474B2 (en) 2020-10-15
IL242092A0 (en) 2015-11-30
US10894940B2 (en) 2021-01-19
MX2019010034A (es) 2019-10-15
ES2752874T3 (es) 2020-04-06
US20150210971A1 (en) 2015-07-30
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