BR112022017298A2 - Microrganismo produtor de l-lisina, sequência polinucleotídica, sequência de aminoácidos, vetores recombinantes, cepas recombinantes, sequência nucleotídica promotora, cassete de expressão e método para produzir l-lisina - Google Patents

Microrganismo produtor de l-lisina, sequência polinucleotídica, sequência de aminoácidos, vetores recombinantes, cepas recombinantes, sequência nucleotídica promotora, cassete de expressão e método para produzir l-lisina

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Publication number
BR112022017298A2
BR112022017298A2 BR112022017298A BR112022017298A BR112022017298A2 BR 112022017298 A2 BR112022017298 A2 BR 112022017298A2 BR 112022017298 A BR112022017298 A BR 112022017298A BR 112022017298 A BR112022017298 A BR 112022017298A BR 112022017298 A2 BR112022017298 A2 BR 112022017298A2
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BR
Brazil
Prior art keywords
lysine
sequence
producing
recombinant
amino acid
Prior art date
Application number
BR112022017298A
Other languages
English (en)
Inventor
Meng Gang
Wei Aiying
Jia Huiping
Ma Fengyong
Zhou Xiaoqun
Zhao Chunguang
Guo Xiaowei
Tian Bin
Gao Xiaohang
Original Assignee
Heilongjiang Eppen Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from CN202010514023.XA external-priority patent/CN111850010B/zh
Priority claimed from CN202010790877.0A external-priority patent/CN111979165B/zh
Application filed by Heilongjiang Eppen Biotech Co Ltd filed Critical Heilongjiang Eppen Biotech Co Ltd
Publication of BR112022017298A2 publication Critical patent/BR112022017298A2/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
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  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

MICRORGANISMO PRODUTOR DE L-LISINA, SEQUÊNCIA POLINUCLEOTÍDICA, SEQUÊNCIA DE AMINOÁCIDOS, VETORES RECOMBINANTES, CEPAS RECOMBINANTES, SEQUÊNCIA NUCLEOTÍDICA PROMOTORA, CASSETE DE EXPRESSÃO E MÉTODO PARA PRODUZIR L-LISINA. A presente invenção provê um método para introduzir mutações pontuais na sequência codificante do gene NCg12176 ou melhorar a sua expressão em Corynebacterium glutamicum e um método para realizar mutações pontuais na sequência da região promotora do gene dapB em Corynebacterium glutamicum. O rendimento da fermentação de L-lisina produzida por uma cepa com as mutações pode ser aumentado por tais métodos.
BR112022017298A 2020-06-08 2020-12-30 Microrganismo produtor de l-lisina, sequência polinucleotídica, sequência de aminoácidos, vetores recombinantes, cepas recombinantes, sequência nucleotídica promotora, cassete de expressão e método para produzir l-lisina BR112022017298A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN202010514023.XA CN111850010B (zh) 2020-06-08 2020-06-08 一种dapB基因改造的重组菌株及其构建方法与应用
CN202010790877.0A CN111979165B (zh) 2020-08-07 2020-08-07 一种产l-赖氨酸的重组菌株及其构建方法与应用
PCT/CN2020/141539 WO2021248890A1 (zh) 2020-06-08 2020-12-30 产l-赖氨酸的重组菌株及其构建方法与应用

Publications (1)

Publication Number Publication Date
BR112022017298A2 true BR112022017298A2 (pt) 2023-02-07

Family

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Family Applications (1)

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BR112022017298A BR112022017298A2 (pt) 2020-06-08 2020-12-30 Microrganismo produtor de l-lisina, sequência polinucleotídica, sequência de aminoácidos, vetores recombinantes, cepas recombinantes, sequência nucleotídica promotora, cassete de expressão e método para produzir l-lisina

Country Status (7)

Country Link
US (1) US20230295645A1 (pt)
EP (1) EP4148120A4 (pt)
JP (1) JP7644133B2 (pt)
KR (1) KR20230002331A (pt)
BR (1) BR112022017298A2 (pt)
MX (1) MX2022013618A (pt)
WO (1) WO2021248890A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115927432A (zh) * 2022-10-17 2023-04-07 齐鲁工业大学 一种产l-氨基酸的谷氨酸棒状杆菌工程菌的构建方法及应用
KR102685485B1 (ko) * 2023-03-20 2024-07-17 대상 주식회사 L-라이신 생산능이 향상된 변이 미생물 및 이를 이용한 l-라이신의 생산 방법
KR20250169496A (ko) * 2024-05-24 2025-12-03 씨제이제일제당 (주) 신규한 단백질 또는 폴리뉴클레오티드 변이체 및 이를 이용한 l-라이신 생산 방법
CN121046285A (zh) * 2025-10-31 2025-12-02 东晓生物科技股份有限公司 一种基于毒素-抗毒素系统编码区突变的l-精氨酸高产菌株、构建方法及应用

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Publication number Priority date Publication date Assignee Title
US6962989B1 (en) * 1999-07-08 2005-11-08 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding novel proteins
JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
WO2003029457A1 (fr) * 2001-09-28 2003-04-10 Kyowa Hakko Kogyo Co., Ltd. Procede de production d'acide amine
JP2013176301A (ja) * 2010-07-01 2013-09-09 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP6335196B2 (ja) 2013-02-08 2018-05-30 ニンシャー エッペン バイオテック カンパニー リミテッド アコニターゼ遺伝子および／またはその調節エレメントの改変により、ｌ−リジンを生産する方法
JP2017131111A (ja) * 2014-06-03 2017-08-03 味の素株式会社 Ｌ−アミノ酸の製造法
CN106367432B (zh) 2016-09-01 2017-07-18 宁夏伊品生物科技股份有限公司 发酵生产l‑赖氨酸的方法及改造的棒杆菌
EP3533875B1 (en) * 2016-09-01 2023-02-15 Ningxia Eppen Biotech Co. Ltd Corynebacterium for producing l-lysine by fermentation
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
RU2019128538A (ru) * 2018-09-26 2021-03-11 Эвоник Оперейшенс ГмбХ Способ ферментативного получения l-лизина
CN111197021B (zh) 2020-01-13 2021-09-21 江南大学 一种l-赖氨酸产量提高的重组谷氨酸棒杆菌及其构建方法
CN111979165B (zh) * 2020-08-07 2021-05-07 黑龙江伊品生物科技有限公司 一种产l-赖氨酸的重组菌株及其构建方法与应用
CN111850010B (zh) * 2020-06-08 2021-04-09 黑龙江伊品生物科技有限公司 一种dapB基因改造的重组菌株及其构建方法与应用

Also Published As

Publication number Publication date
EP4148120A4 (en) 2024-10-02
WO2021248890A1 (zh) 2021-12-16
US20230295645A1 (en) 2023-09-21
JP7644133B2 (ja) 2025-03-11
KR20230002331A (ko) 2023-01-05
EP4148120A1 (en) 2023-03-15
MX2022013618A (es) 2022-11-16
JP2023527951A (ja) 2023-07-03

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