BR9912014A - Célula microbiana, composição, sequência de nucleotìdeo, replicon de dna recombinante, uso da célula microbiana, processo de produção de um primeiro metabólico, processo de construção de uma célula microbiana - Google Patents
Célula microbiana, composição, sequência de nucleotìdeo, replicon de dna recombinante, uso da célula microbiana, processo de produção de um primeiro metabólico, processo de construção de uma célula microbianaInfo
- Publication number
- BR9912014A BR9912014A BR9912014-3A BR9912014A BR9912014A BR 9912014 A BR9912014 A BR 9912014A BR 9912014 A BR9912014 A BR 9912014A BR 9912014 A BR9912014 A BR 9912014A
- Authority
- BR
- Brazil
- Prior art keywords
- microbial cell
- sub
- increased
- activity
- enzyme activity
- Prior art date
Links
- 230000000813 microbial effect Effects 0.000 title abstract 4
- 108020004511 Recombinant DNA Proteins 0.000 title 1
- 230000002503 metabolic effect Effects 0.000 title 1
- 239000002773 nucleotide Substances 0.000 title 1
- 125000003729 nucleotide group Chemical group 0.000 title 1
- 230000000694 effects Effects 0.000 abstract 7
- 102000004190 Enzymes Human genes 0.000 abstract 4
- 108090000790 Enzymes Proteins 0.000 abstract 4
- 108091026890 Coding region Proteins 0.000 abstract 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 3
- 229930195712 glutamate Natural products 0.000 abstract 3
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 abstract 2
- 229910021529 ammonia Inorganic materials 0.000 abstract 1
- 230000001195 anabolic effect Effects 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
<B>"CéLULA MICROBIANA, COMPOSIçãO, SEQUêNCIA DE NUCLEOTìDEO, REPLICON DE DNA RECOMBINANTE, USO DA CéLULA MICROBIANA, PROCESSO DE PRODUçãO DE UM PRIMEIRO METABóLITO, PROCESSO DE CONSTRUçãO DE UMA CéLULA MICROBIANA"<D>. A invenção diz respeito a uma manipulação da assimilação de uma fonte de nutriente em uma célula microbiana. A fonte de nutriente é preferivelmente amónia e a captação desta na célula microbiana, preferivelmente uma célula de levedura, está articulada a uma produção alterada de um metabólito tal como etanol ou gliceral. As células microbianas de acordo com a invenção compreendem uma primeira atividade exprimível da enzima que, quando manisfestada na célula microbiana, está controlando a assimilação na célula de uma fonte de nutriente, e opcionalmente uma segunda atividade exprimível de enzima que, quando manifestada na célula microbiana, está controlando a assimilação na célula de uma fonte de nutriente, a segunda atividade exprimível da enzima sendo diferente da primeira atividade exprimível da enzima, e uma expressão ou não expressão de uma terceira atividade exprimível da enzima que, quando manifestada na célula microbiana, está controlando a assimilação na célula de uma fonte de nutriente, a terceira atividade exprimível da enzima sendo diferente a qualquer uma, tanto da primeira quanto segunda atividades exprimíveis da enzima. As células de acordo com a invenção são capazes de produzir quantidades aumentadas de etanol em uma fermentação.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199800967 | 1998-07-10 | ||
| DKPA199800967 | 1998-07-10 | ||
| PCT/DK1999/000397 WO2000003020A1 (en) | 1998-07-10 | 1999-07-12 | Metabolically engineered microbial cell with an altered metabolite production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR9912014A true BR9912014A (pt) | 2001-04-10 |
| BR9912014B1 BR9912014B1 (pt) | 2010-03-23 |
Family
ID=8099516
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9912014-3A BR9912014B1 (pt) | 1998-07-10 | 1999-07-12 | cÉlula microbiana recombinante, e, processos de produÇço de um primeiro metabàlito e de construÇço de uma cÉlula microbiana. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7018829B1 (pt) |
| EP (1) | EP1097216B1 (pt) |
| AT (1) | ATE325196T1 (pt) |
| AU (1) | AU773874B2 (pt) |
| BR (1) | BR9912014B1 (pt) |
| CA (1) | CA2379023C (pt) |
| DE (1) | DE69931162T2 (pt) |
| ES (1) | ES2267274T3 (pt) |
| NZ (1) | NZ509728A (pt) |
| WO (1) | WO2000003020A1 (pt) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4744115B2 (ja) * | 2004-09-13 | 2011-08-10 | トヨタ自動車株式会社 | 乳酸の製造方法 |
| US20070249018A1 (en) * | 2006-02-23 | 2007-10-25 | Goutham Vemuri | Reduced overflow metabolism and methods of use |
| WO2009053489A1 (de) * | 2007-10-27 | 2009-04-30 | Evonik Degussa Gmbh | Fermentative herstellung von alpha-ketoglutarsäure |
| EP2060632A1 (en) * | 2007-10-29 | 2009-05-20 | Technische Universität Berlin | Method of modifying a yeast cell for the production of ethanol |
| US8431374B2 (en) | 2007-10-31 | 2013-04-30 | Gevo, Inc. | Methods for the economical production of biofuel from biomass |
| CA2641003C (en) * | 2008-03-20 | 2012-01-03 | Greenfield Ethanol Inc. | Genetically modified yeast strain |
| US20110229949A1 (en) * | 2008-03-20 | 2011-09-22 | Greenfield Ethanol Inc. | Genetically modified yeast strain |
| WO2010051527A2 (en) | 2008-10-31 | 2010-05-06 | Gevo, Inc. | Engineered microorganisms capable of producing target compounds under anaerobic conditions |
| CA2765849A1 (en) | 2009-07-02 | 2011-01-06 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| US9605269B2 (en) * | 2010-05-05 | 2017-03-28 | Lallemand Hungary Liquidity Management Llc | Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate |
| US8728798B2 (en) | 2011-05-03 | 2014-05-20 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| US8343752B2 (en) | 2011-05-03 | 2013-01-01 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| US10604772B2 (en) | 2013-03-15 | 2020-03-31 | Lallemand Hungary Liquidity Management Llc | Methods for regulating nitrogen metabolism during the production of ethanol from corn by metabolically engineered yeast strains |
| CN104560841A (zh) * | 2013-10-18 | 2015-04-29 | 常州邱鸿生物技术有限公司 | 一种重组大肠杆菌利用甘油生产3-羟基丁酮的方法 |
| WO2018144807A2 (en) * | 2017-02-02 | 2018-08-09 | Massachusetts Institute Of Technology | Engineered yeast as a method for bioremediation |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL80510A0 (en) * | 1985-11-08 | 1987-02-27 | Genetics Inst | Improved yeast strains |
| RU2182173C2 (ru) | 1993-10-28 | 2002-05-10 | Адзиномото Ко., Инк. | Способ получения l-аминокислоты |
| DK0939830T3 (da) * | 1996-09-06 | 2009-04-06 | Peter Ruhdal Jensen | Fremgangsmåde til forbedring af produktionen af et önsket produkt fra en celle |
| GB9622516D0 (en) * | 1996-10-29 | 1997-01-08 | Univ Cambridge Tech | Enzymic cofactor cycling |
-
1999
- 1999-07-12 US US09/743,414 patent/US7018829B1/en not_active Expired - Lifetime
- 1999-07-12 NZ NZ509728A patent/NZ509728A/xx not_active IP Right Cessation
- 1999-07-12 EP EP99932682A patent/EP1097216B1/en not_active Expired - Lifetime
- 1999-07-12 AU AU48978/99A patent/AU773874B2/en not_active Ceased
- 1999-07-12 DE DE69931162T patent/DE69931162T2/de not_active Expired - Lifetime
- 1999-07-12 ES ES99932682T patent/ES2267274T3/es not_active Expired - Lifetime
- 1999-07-12 WO PCT/DK1999/000397 patent/WO2000003020A1/en not_active Ceased
- 1999-07-12 BR BRPI9912014-3A patent/BR9912014B1/pt not_active IP Right Cessation
- 1999-07-12 AT AT99932682T patent/ATE325196T1/de not_active IP Right Cessation
- 1999-07-12 CA CA2379023A patent/CA2379023C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US7018829B1 (en) | 2006-03-28 |
| ES2267274T3 (es) | 2007-03-01 |
| DE69931162T2 (de) | 2007-03-29 |
| WO2000003020A1 (en) | 2000-01-20 |
| CA2379023C (en) | 2010-05-18 |
| ATE325196T1 (de) | 2006-06-15 |
| AU773874B2 (en) | 2004-06-10 |
| BR9912014B1 (pt) | 2010-03-23 |
| AU4897899A (en) | 2000-02-01 |
| CA2379023A1 (en) | 2000-01-20 |
| EP1097216A1 (en) | 2001-05-09 |
| NZ509728A (en) | 2003-07-25 |
| DE69931162D1 (de) | 2006-06-08 |
| EP1097216B1 (en) | 2006-05-03 |
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Free format text: FLUXOME SCIENCES A/S (DK) |
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