BR9916724A - Produção de monelina recombinante utilizando sistema de expressão em leveduras metilotróficas - Google Patents
Produção de monelina recombinante utilizando sistema de expressão em leveduras metilotróficasInfo
- Publication number
- BR9916724A BR9916724A BR9916724-7A BR9916724A BR9916724A BR 9916724 A BR9916724 A BR 9916724A BR 9916724 A BR9916724 A BR 9916724A BR 9916724 A BR9916724 A BR 9916724A
- Authority
- BR
- Brazil
- Prior art keywords
- monelin
- recombinant
- protein
- production
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/43—Sweetening agents, e.g. thaumatin, monellin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Patente de Invenção: <B>"PRODUçãO DE MONELINA RECOMBINANTE UTILIZANDO SISTEMA DE EXPRESSãO EM LEVEDURAS METILOTRóFICAS"<D>. A presente invenção refere-se a uma proteína semelhante à monelina de cadeia única que é estável e que é pelo menos 100 vezes mais doce quando comparada com a sacarose com base em peso. A presente invenção também refere-se a um ácido nucléico que codifica a dita proteína semelhante à monelina. Preferencialmente, o ácido nucléico compreende ainda um promotor e uma seq³ência sinal para o direcionamento da expressão e da secreção da proteína semelhante à monelina codificada na levedura metilotrófica Pichia pastoris. A presente invenção refere-se ainda a uma célula de Pichia pastoris recombinante que contém o ácido nucléico que codifica a proteína semelhante à monelina, a um processo para obtenção da proteína semelhante à monelina da Pichia pastoris recombinante e ao produto do processo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11452998P | 1998-12-31 | 1998-12-31 | |
| PCT/US1999/029213 WO2000040603A2 (en) | 1998-12-31 | 1999-12-09 | Production of recombinant monellin using methylotrophic yeast expression system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR9916724A true BR9916724A (pt) | 2001-09-11 |
Family
ID=22355787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9916724-7A BR9916724A (pt) | 1998-12-31 | 1999-12-09 | Produção de monelina recombinante utilizando sistema de expressão em leveduras metilotróficas |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1141304A2 (pt) |
| JP (1) | JP2002534074A (pt) |
| KR (1) | KR20020008819A (pt) |
| CN (1) | CN1332802A (pt) |
| AU (1) | AU3116100A (pt) |
| BR (1) | BR9916724A (pt) |
| CA (1) | CA2364005A1 (pt) |
| IL (1) | IL143771A0 (pt) |
| WO (1) | WO2000040603A2 (pt) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023222111A1 (zh) * | 2022-05-20 | 2023-11-23 | 南京百斯杰生物工程有限公司 | 具有高甜度的甜味蛋白莫内林(Monellin)突变体及其制备方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3998798A (en) * | 1972-12-22 | 1976-12-21 | The Trustees Of The University Of Pennsylvania | Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii |
| DE3850574T2 (de) * | 1987-06-19 | 1994-10-27 | Lucky Ltd | Neue klasse von niedrigkalorischen proteinsüssstoffen. |
| US6001410A (en) * | 1996-07-25 | 1999-12-14 | International Flavors & Fragrances Inc. | Fruit liqueur beverage containing recombinant monellin to enhance the alcoholic impact |
-
1999
- 1999-12-09 EP EP99965192A patent/EP1141304A2/en not_active Withdrawn
- 1999-12-09 AU AU31161/00A patent/AU3116100A/en not_active Abandoned
- 1999-12-09 JP JP2000592311A patent/JP2002534074A/ja not_active Withdrawn
- 1999-12-09 IL IL14377199A patent/IL143771A0/xx unknown
- 1999-12-09 BR BR9916724-7A patent/BR9916724A/pt not_active Application Discontinuation
- 1999-12-09 CN CN99815165A patent/CN1332802A/zh active Pending
- 1999-12-09 KR KR1020017008413A patent/KR20020008819A/ko not_active Withdrawn
- 1999-12-09 CA CA002364005A patent/CA2364005A1/en active Pending
- 1999-12-09 WO PCT/US1999/029213 patent/WO2000040603A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU3116100A (en) | 2000-07-24 |
| JP2002534074A (ja) | 2002-10-15 |
| KR20020008819A (ko) | 2002-01-31 |
| IL143771A0 (en) | 2002-04-21 |
| CA2364005A1 (en) | 2000-07-13 |
| EP1141304A2 (en) | 2001-10-10 |
| WO2000040603A2 (en) | 2000-07-13 |
| CN1332802A (zh) | 2002-01-23 |
| WO2000040603A3 (en) | 2000-09-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA10 | Dismissal: dismissal - article 33 of industrial property law | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |