BRPI0109674A - método de concentração e de recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica, e, embalagem comercial - Google Patents

método de concentração e de recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica, e, embalagem comercial

Info

Publication number
BRPI0109674A
BRPI0109674A BRPI0109674A BRPI0109674A BRPI0109674A BR PI0109674 A BRPI0109674 A BR PI0109674A BR PI0109674 A BRPI0109674 A BR PI0109674A BR PI0109674 A BRPI0109674 A BR PI0109674A BR PI0109674 A BRPI0109674 A BR PI0109674A
Authority
BR
Brazil
Prior art keywords
enzyme activity
biological sample
buffer solution
enveloped viruses
matrix
Prior art date
Application number
BRPI0109674A
Other languages
English (en)
Inventor
Anders Malmsten
Clas Kaellander
Ingvar Pettersson
Simon Gronowitz
Tommy Gatu
Original Assignee
Cavidi Tech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cavidi Tech Ab filed Critical Cavidi Tech Ab
Publication of BRPI0109674A publication Critical patent/BRPI0109674A/pt
Publication of BRPI0109674B1 publication Critical patent/BRPI0109674B1/pt
Publication of BRPI0109674B8 publication Critical patent/BRPI0109674B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

"método de concentração e de recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica, e, embalagem comercial". e descrito um método de concentração e de recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica. o método compreende por em contato a amostra biológica em uma primeira solução tamponadora com uma matriz de ligação de vírus, tal como uma matriz de troca aniônica, para fazer aderir partículas de vírus presentes na amostra à matriz, lavar a matriz que contém as partículas de vírus com uma segunda solução tamponadora para remover componentes que interfiram na atividade viral da enzima, lisar as partículas imobilizadas de vírus em uma terceira solução tamponadora e recuperar a atividade viral da enzima concentrada da terceira solução tamponadora. adicionalmente, é divulgada uma embalagem comercial que contém instruções escritas e/ou dados do veículo para a realização de etapas de laboratório para concentração e recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica e pelo menos um componente necessário para o ensaio.
BRPI0109674 2000-03-29 2001-03-22 Método de concentração e de recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica, e, embalagem comercial BRPI0109674B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0001132A SE0001132D0 (sv) 2000-03-29 2000-03-29 Method of concentrating and recovering a viral enzyme activity from biological samples
PCT/SE2001/000617 WO2001075147A1 (en) 2000-03-29 2001-03-22 Method of concentrating and recovering a viral enzyme activity from biological samples

Publications (3)

Publication Number Publication Date
BRPI0109674A true BRPI0109674A (pt) 2003-02-04
BRPI0109674B1 BRPI0109674B1 (pt) 2014-02-11
BRPI0109674B8 BRPI0109674B8 (pt) 2021-07-06

Family

ID=20279073

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0109674 BRPI0109674B8 (pt) 2000-03-29 2001-03-22 Método de concentração e de recuperação de uma atividade de enzima de vírus envelopados presentes em uma amostra biológica, e, embalagem comercial

Country Status (12)

Country Link
US (1) US7833700B2 (pt)
EP (1) EP1268851B1 (pt)
CN (1) CN1204265C (pt)
AT (1) ATE307897T1 (pt)
AU (1) AU2001244921A1 (pt)
BR (1) BRPI0109674B8 (pt)
DE (1) DE60114389T2 (pt)
DK (1) DK1268851T3 (pt)
ES (1) ES2252206T3 (pt)
HK (1) HK1053855B (pt)
SE (1) SE0001132D0 (pt)
WO (1) WO2001075147A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0101219D0 (sv) 2001-04-05 2001-04-05 Cavidi Tech Ab Recovery of enzyme activity from enveloped viruses
US7875422B2 (en) * 2001-06-14 2011-01-25 Cavidi Ab Viral drug susceptibility testing
DE10230819B4 (de) * 2002-07-08 2007-12-06 Kronotec Ag Fussbodenpaneel
WO2004042003A2 (en) 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482834A (en) * 1982-05-17 1996-01-09 Hahnemann University Evaluation of nucleic acids in a biological sample hybridization in a solution of chaotrophic salt solubilized cells
US4707439A (en) * 1984-10-26 1987-11-17 The United States Of America As Represented By The Department Of Health And Human Services Screening test for reverse-transcriptase containing virus such as non-A, non-B hepatitis, NANBH
CA2107798C (en) * 1992-02-10 1996-04-02 John R. Chapman Method for testing blood units for viral contamination
US5658779A (en) * 1992-03-20 1997-08-19 Ligochem, Inc. Method of adsorbing viruses from fluid compositions
DE69515028T2 (de) * 1994-03-22 2000-06-29 The Immune Response Corp., Carlsbad Die hocheffiziente herstellung und isolierung von viruspartikeln
SE9902410D0 (sv) * 1999-06-24 1999-06-24 Cavidi Tech Ab Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples

Also Published As

Publication number Publication date
ES2252206T3 (es) 2006-05-16
WO2001075147A1 (en) 2001-10-11
EP1268851A1 (en) 2003-01-02
HK1053855A1 (en) 2003-11-07
DK1268851T3 (da) 2006-03-13
US7833700B2 (en) 2010-11-16
EP1268851B1 (en) 2005-10-26
BRPI0109674B1 (pt) 2014-02-11
US20030104441A1 (en) 2003-06-05
BRPI0109674B8 (pt) 2021-07-06
SE0001132D0 (sv) 2000-03-29
DE60114389D1 (de) 2005-12-01
CN1204265C (zh) 2005-06-01
HK1053855B (en) 2006-09-01
AU2001244921A1 (en) 2001-10-15
DE60114389T2 (de) 2006-07-13
ATE307897T1 (de) 2005-11-15
CN1419605A (zh) 2003-05-21

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Legal Events

Date Code Title Description
B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

Free format text: PATENTE EXTINTA EM 22/03/2021