BRPI0313559B8 - método para o cultivo de células primárias e para a amplificação de vírus sob condições isentas de soro - Google Patents

método para o cultivo de células primárias e para a amplificação de vírus sob condições isentas de soro

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Publication number
BRPI0313559B8
BRPI0313559B8 BRPI0313559A BR0313559A BRPI0313559B8 BR PI0313559 B8 BRPI0313559 B8 BR PI0313559B8 BR PI0313559 A BRPI0313559 A BR PI0313559A BR 0313559 A BR0313559 A BR 0313559A BR PI0313559 B8 BRPI0313559 B8 BR PI0313559B8
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BR
Brazil
Prior art keywords
primary cells
cultivation
amplification
free conditions
under serum
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BRPI0313559A
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English (en)
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BR0313559A (pt
BRPI0313559B1 (pt
Inventor
Felder Eva
Räther Ingmar
Heller Karl
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Bavarian Nordic As
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31970216&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0313559(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bavarian Nordic As filed Critical Bavarian Nordic As
Publication of BR0313559A publication Critical patent/BR0313559A/pt
Publication of BRPI0313559B1 publication Critical patent/BRPI0313559B1/pt
Publication of BRPI0313559B8 publication Critical patent/BRPI0313559B8/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C12N7/08—Inactivation or attenuation by serial passage of virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A61K39/285—Vaccinia virus or variola virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2510/00—Genetically modified cells
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
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  • Medicinal Chemistry (AREA)
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  • Reproductive Health (AREA)
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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"método para o cultivo de células primárias e para a amplificação de vírus sob condições isentas de soro". a presente invenção refere-se a um método para o cultivo de células primárias. as células primárias são cultivadas em um meio isento de soro que compreende um fator selecionado do grupo que consiste em fatores de crescimento e em fatores de adesão. o processo para o cultivo de células primárias pode ser uma etapa em um método para a amplificação de vírus, tais como os poxvírus. de acordo com este último método, as células primárias são cultivadas em um meio isento de soro que compreende um fator selecionado do grupo que consiste em fatores de crescimento e em fatores de adesão. as células são então infectadas com o vírus e as células infectadas são cultivadas em um meio isento de soro até que uma progênie viral seja produzida.
BRPI0313559A 2002-09-05 2003-09-01 método para o cultivo de células primárias e para a amplificação de vírus sob condições isentas de soro BRPI0313559B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200201302 2002-09-05
DKPA200201302 2002-09-05
PCT/EP2003/009704 WO2004022729A1 (en) 2002-09-05 2003-09-01 Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions

Publications (3)

Publication Number Publication Date
BR0313559A BR0313559A (pt) 2005-07-12
BRPI0313559B1 BRPI0313559B1 (pt) 2019-02-26
BRPI0313559B8 true BRPI0313559B8 (pt) 2021-05-25

Family

ID=31970216

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0313559A BRPI0313559B8 (pt) 2002-09-05 2003-09-01 método para o cultivo de células primárias e para a amplificação de vírus sob condições isentas de soro

Country Status (23)

Country Link
US (4) US7695939B2 (pt)
EP (1) EP1434858B2 (pt)
JP (2) JP2005537793A (pt)
KR (1) KR101044538B1 (pt)
CN (1) CN1692156B (pt)
AT (1) ATE393212T2 (pt)
AU (1) AU2003264144C1 (pt)
BR (1) BRPI0313559B8 (pt)
CA (1) CA2494379C (pt)
CY (1) CY1114598T1 (pt)
DE (1) DE60320520T3 (pt)
DK (1) DK1434858T4 (pt)
EA (1) EA009008B1 (pt)
ES (1) ES2305514T5 (pt)
HK (1) HK1080507B (pt)
MX (1) MXPA04012966A (pt)
NO (1) NO343489B1 (pt)
NZ (1) NZ538575A (pt)
PL (1) PL215169B1 (pt)
PT (1) PT1434858E (pt)
SI (1) SI1434858T2 (pt)
UA (1) UA85543C2 (pt)
WO (1) WO2004022729A1 (pt)

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