BRPI0408852A - processo para a coloração de espermatozóide - Google Patents

processo para a coloração de espermatozóide

Info

Publication number
BRPI0408852A
BRPI0408852A BRPI0408852-2A BRPI0408852A BRPI0408852A BR PI0408852 A BRPI0408852 A BR PI0408852A BR PI0408852 A BRPI0408852 A BR PI0408852A BR PI0408852 A BRPI0408852 A BR PI0408852A
Authority
BR
Brazil
Prior art keywords
sperm
coloring
cells
sperm cells
involve
Prior art date
Application number
BRPI0408852-2A
Other languages
English (en)
Inventor
Muhammad Anzar
Cindy L Ludwig
Jeffrey A Graham
Jeannet A Glaenzer
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BRPI0408852A publication Critical patent/BRPI0408852A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Reproductive Health (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

"PROCESSO PARA A COLORAçãO DE ESPERMATOZóIDE". A presente invenção refere-se a células de espermatozóide que são coradas de acordo com processos que envolvem a combinação de células de espermatozóide com um corante seletivo para DNA fluorescente em uma temperatura elevada em excesso de cerca de 40EC. Os métodos possibilitam uma redução do tempo de coloração. As células depois disso podem ser eficientemente classificadas de acordo com métodos comuns de separação, inclusive citometria de fluxo.
BRPI0408852-2A 2003-03-28 2004-03-29 processo para a coloração de espermatozóide BRPI0408852A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45873103P 2003-03-28 2003-03-28
PCT/US2004/009903 WO2004087177A1 (en) 2003-03-28 2004-03-29 Process for the staining of sperm

Publications (1)

Publication Number Publication Date
BRPI0408852A true BRPI0408852A (pt) 2006-04-04

Family

ID=33131822

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0408852-2A BRPI0408852A (pt) 2003-03-28 2004-03-29 processo para a coloração de espermatozóide

Country Status (14)

Country Link
US (1) US7335507B2 (pt)
EP (1) EP1608382B1 (pt)
JP (1) JP2006525019A (pt)
CN (1) CN100379429C (pt)
AT (1) ATE369867T1 (pt)
AU (1) AU2004226343B2 (pt)
BR (1) BRPI0408852A (pt)
CA (1) CA2519989A1 (pt)
DE (1) DE602004008241T2 (pt)
MX (1) MXPA05010478A (pt)
NZ (1) NZ542688A (pt)
PL (1) PL1608382T3 (pt)
WO (1) WO2004087177A1 (pt)
ZA (1) ZA200507823B (pt)

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US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
IL152714A (en) 2000-05-09 2014-03-31 Xy Llc High-purity spermatozoa populations carrying chromosome-x and chromosome-y
CN1633259A (zh) * 2000-06-12 2005-06-29 Xy公司 利用分离的带x－染色体和带y－染色体的精子群的综合兽群管理系统
US20040031071A1 (en) * 2000-10-05 2004-02-12 Xy, Inc. System of hysteroscopic insemination of mares
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ，エルエルシー 低圧精子細胞分離システム
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) * 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
BRPI0408857B1 (pt) 2003-03-28 2018-09-11 Inguran Llc aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
WO2004087177A1 (en) 2003-03-28 2004-10-14 Monsanto Technology Llc Process for the staining of sperm
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
DK2801363T3 (en) * 2004-03-29 2018-05-28 Inguran Llc PROCEDURE FOR STORING SORTED SPERMATOZOES
DE602005018907D1 (de) * 2004-03-29 2010-03-04 Inguran Llc Verwendung einer oxidations-/reduktionsreaktionen intrazellulär und/oder extrazellulär regulierenden zusammensetzung in einem anfärbe- oder sortierungsverfahren für spermatozoen
CA2574499C (en) * 2004-07-22 2016-11-29 Monsanto Technology Llc Process for enriching a population of sperm cells
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
KR100949923B1 (ko) * 2008-04-21 2010-03-30 중앙대학교 산학협력단 번식능력이 불량한 수퇘지 선별법
US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
CA2794934A1 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2012167151A1 (en) 2011-06-01 2012-12-06 Inguran, Llc Compositions and methods for improving the quality of processed sperm
US9140688B2 (en) 2011-09-30 2015-09-22 Inguran, Llc Sperm staining and sorting methods
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CA2886782C (en) 2012-10-05 2020-03-10 Inguran, Llc High efficiency methods of sex sorting sperm
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
US11413358B2 (en) 2018-04-09 2022-08-16 Inguran, Llc Sperm nuclei and methods of their manufacture and use
US10603075B1 (en) 2018-11-30 2020-03-31 Ohana Biosciences, Inc. Compositions and methods for enhancing sperm function
CN116348063A (zh) * 2020-10-15 2023-06-27 Abs全球公司 猪的选性精液和其使用方法

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US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
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Also Published As

Publication number Publication date
EP1608382A1 (en) 2005-12-28
AU2004226343A1 (en) 2004-10-14
ZA200507823B (en) 2008-03-26
US7335507B2 (en) 2008-02-26
US20050003472A1 (en) 2005-01-06
ATE369867T1 (de) 2007-09-15
CA2519989A1 (en) 2004-10-14
CN1795004A (zh) 2006-06-28
EP1608382B1 (en) 2007-08-15
MXPA05010478A (es) 2005-12-15
JP2006525019A (ja) 2006-11-09
PL1608382T3 (pl) 2008-03-31
CN100379429C (zh) 2008-04-09
NZ542688A (en) 2008-06-30
DE602004008241D1 (de) 2007-09-27
DE602004008241T2 (de) 2008-05-08
WO2004087177A1 (en) 2004-10-14
AU2004226343B2 (en) 2008-10-09

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: INGURAN LLC (US)

Free format text: TRANSFERIDO DE: MONSANTO TECHNOLOGY LLC

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 6A, 7A E 8A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2159 DE 22/05/2012.

B15K Others concerning applications: alteration of classification

Ipc: C12N 5/071 (2010.01)