BRPI0409178A - method for producing transgenic plants and modulating cytokine activity in plants, transgenic plants, dna recombinate, promoter and expression cassette - Google Patents

method for producing transgenic plants and modulating cytokine activity in plants, transgenic plants, dna recombinate, promoter and expression cassette

Info

Publication number
BRPI0409178A
BRPI0409178A BRPI0409178-7A BRPI0409178A BRPI0409178A BR PI0409178 A BRPI0409178 A BR PI0409178A BR PI0409178 A BRPI0409178 A BR PI0409178A BR PI0409178 A BRPI0409178 A BR PI0409178A
Authority
BR
Brazil
Prior art keywords
plants
plant
transgenic plants
promoter
expression
Prior art date
Application number
BRPI0409178-7A
Other languages
Portuguese (pt)
Inventor
Jeffrey E Habben
Chris Zinselmeier
Dwight T Tomes
Shane E Abbitt
Timothy G Helentjaris
Xiaomu Niu
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of BRPI0409178A publication Critical patent/BRPI0409178A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"MéTODO PARA PRODUZIR PLANTAS TRANSGêNICAS E PARA MODULAR A ATIVIDADE DE CITOCININA EM PLANTAS, PLANTAS TRANSGêNICAS, DNA RECOMBINANTE, PROMOTOR E CASSETE DE EXPRESSãO". Esta invenção se refere, em geral, ao campo de biologia molecular de plantas. Mais especificamente, esta invenção se refere a métodos e reagentes para a expressão temporalmente- e/ou espacialmente-regulada de genes que afetam metabolicamente os níveis efetivos de citocininas em plantas, particularmente em sementes de planta e tecido reprodutivo feminino relacionado. Esta invenção adicionalmente se refere a plantas transgênicas tendo níveis melhorados de expressão de citocinina, sendo que a planta transgênica apresenta características úteis, tais como tamanho de semente melhorado, aborto de extremidade de grão diminuído, aumentada produção de semente durante condições ambientais desfavoráveis, ou estabilidade de produtividade. A presente invenção também provê composições e métodos para regular a expressão de seqüências de nucleotídeos heterólogas em uma planta. As composições compreendem novas seqüências de nucleotídeos para promotores semente-preferidos conhecidos como eep1 e eep2. é provido um método para expressar uma seqüência de nucleotídeos heteróloga em uma planta usando as seqüências de promotor reveladas aqui. O método compreende a transformação de uma célula de planta para compreender uma seqüência de nucleotídeos heteróloga operacionalmente ligada a um dos promotores da presente invenção e regeneração de uma planta estavelmente transformada a partir da célula de planta transformada."METHOD FOR PRODUCING TRANSGENIC PLANTS AND MODULATING CYTOKININE ACTIVITY IN PLANTS, TRANSGENIC PLANTS, RECOMBINANT DNA, PROMOTER AND EXPRESSION CASSETTE". This invention generally relates to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and / or spatially-regulated expression of genes that metabolically affect effective cytokine levels in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having improved levels of cytokine expression, the transgenic plant having useful characteristics such as improved seed size, decreased grain end miscarriage, increased seed yield during unfavorable environmental conditions, or stability. productivity The present invention also provides compositions and methods for regulating the expression of heterologous nucleotide sequences in a plant. The compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method is provided for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

BRPI0409178-7A 2003-04-04 2004-04-02 method for producing transgenic plants and modulating cytokine activity in plants, transgenic plants, dna recombinate, promoter and expression cassette BRPI0409178A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46071803P 2003-04-04 2003-04-04
PCT/US2004/010064 WO2004090143A2 (en) 2003-04-04 2004-04-02 Modulation of cytokinin activity in plants

Publications (1)

Publication Number Publication Date
BRPI0409178A true BRPI0409178A (en) 2006-05-02

Family

ID=33159802

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0409178-7A BRPI0409178A (en) 2003-04-04 2004-04-02 method for producing transgenic plants and modulating cytokine activity in plants, transgenic plants, dna recombinate, promoter and expression cassette

Country Status (9)

Country Link
EP (1) EP1613754A2 (en)
CN (3) CN1798843A (en)
AR (1) AR045420A1 (en)
AU (1) AU2004227360B2 (en)
BR (1) BRPI0409178A (en)
CA (1) CA2521497C (en)
NZ (1) NZ543070A (en)
WO (1) WO2004090143A2 (en)
ZA (1) ZA200508006B (en)

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Also Published As

Publication number Publication date
CN1798843A (en) 2006-07-05
ZA200508006B (en) 2007-01-31
NZ543070A (en) 2008-10-31
CN101220358A (en) 2008-07-16
WO2004090143A3 (en) 2005-05-12
AR045420A1 (en) 2005-10-26
CA2521497C (en) 2012-11-27
AU2004227360A1 (en) 2004-10-21
EP1613754A2 (en) 2006-01-11
AU2004227360B2 (en) 2009-05-28
CN101220357A (en) 2008-07-16
WO2004090143A2 (en) 2004-10-21
CA2521497A1 (en) 2004-10-21

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

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