BRPI0410510A - moléculas de ácido nucléico isolada, gene quimérico, yarrowia sp. transformada, métodos de produção de ácidos y-linolênico, estearidÈnico, eicosatetraenóico, eicosapentaenóico, di-homo-y-linoléico, docosapentaenóico, método de otimização de um gene, métodos de otimização da expressão de um gene e óleo microbiano - Google Patents

moléculas de ácido nucléico isolada, gene quimérico, yarrowia sp. transformada, métodos de produção de ácidos y-linolênico, estearidÈnico, eicosatetraenóico, eicosapentaenóico, di-homo-y-linoléico, docosapentaenóico, método de otimização de um gene, métodos de otimização da expressão de um gene e óleo microbiano

Info

Publication number
BRPI0410510A
BRPI0410510A BRPI0410510-9A BRPI0410510A BRPI0410510A BR PI0410510 A BRPI0410510 A BR PI0410510A BR PI0410510 A BRPI0410510 A BR PI0410510A BR PI0410510 A BRPI0410510 A BR PI0410510A
Authority
BR
Brazil
Prior art keywords
gene
acid
linolenic
methods
dihomo
Prior art date
Application number
BRPI0410510-9A
Other languages
English (en)
Inventor
Stephen K Picataggio
Quinn Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BRPI0410510A publication Critical patent/BRPI0410510A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"MOLéCULAS DE áCIDO NUCLéICO ISOLADA, GENE QUIMéRICO, YARROWIA SP. TRANSFORMADA, MéTODOS DE PRODUçãO DE áCIDOS <sym>-LINOLêNICO, ESTEARIDÈNICO, EICOSATETRAENóICO, EICOSAPENTAENóICO, DI-HOMO-<sym>-LINOLéICO, DOCOSAPENTAENóICO, MéTODO DE OTIMIZAçãO DE UM GENE, MéTODOS DE OTIMIZAçãO DA EXPRESSãO DE UM GENE E óLEO MICROBIANO". A presente invenção refere-se a elongases e dessaturases de ácidos graxos capazes de catalisar a conversão de ácido linoléico (LA) em ácido <sym>-linolênico (GLA); ácido <244>-linoléico (ALA) em ácido estearidónico (STA); GLA em ácido di-homo-<sym>-linoléico (DGLA); STA em ácido eicosatetraenóico (ETA): DGLA em ETA; ácido eicosapentaenóico (EPA) em ácido docosapentaenóico (DPA); e ácido araquidónico (ARA) em EPA. São descritas seqüências de ácidos nucléicos que codificam elongases e dessaturases otimizadas por códons, seqüências de ácidos nucléicos que se hibridizam a elas, construções de DNA que compreendem a elongase ou dessaturase otimizada por códon e microorganismos hospedeiros recombinates que expressam níveis maiores de dessaturase ou elongase.
BRPI0410510-9A 2003-05-07 2004-05-07 moléculas de ácido nucléico isolada, gene quimérico, yarrowia sp. transformada, métodos de produção de ácidos y-linolênico, estearidÈnico, eicosatetraenóico, eicosapentaenóico, di-homo-y-linoléico, docosapentaenóico, método de otimização de um gene, métodos de otimização da expressão de um gene e óleo microbiano BRPI0410510A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US46867703P 2003-05-07 2003-05-07
US46871803P 2003-05-07 2003-05-07
PCT/US2004/014442 WO2004101753A2 (en) 2003-05-07 2004-05-07 Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts

Publications (1)

Publication Number Publication Date
BRPI0410510A true BRPI0410510A (pt) 2006-06-20

Family

ID=33457099

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0410510-9A BRPI0410510A (pt) 2003-05-07 2004-05-07 moléculas de ácido nucléico isolada, gene quimérico, yarrowia sp. transformada, métodos de produção de ácidos y-linolênico, estearidÈnico, eicosatetraenóico, eicosapentaenóico, di-homo-y-linoléico, docosapentaenóico, método de otimização de um gene, métodos de otimização da expressão de um gene e óleo microbiano

Country Status (8)

Country Link
US (4) US7125672B2 (pt)
EP (1) EP1620542A4 (pt)
JP (1) JP2007504838A (pt)
KR (1) KR20060018833A (pt)
BR (1) BRPI0410510A (pt)
CA (1) CA2522692A1 (pt)
NO (1) NO20055785L (pt)
WO (1) WO2004101753A2 (pt)

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KR20060018833A (ko) 2006-03-02
JP2007504838A (ja) 2007-03-08
US7511128B2 (en) 2009-03-31
US7838651B2 (en) 2010-11-23
NO20055785D0 (no) 2005-12-06
US20080154027A1 (en) 2008-06-26
EP1620542A4 (en) 2007-02-14
US20040253621A1 (en) 2004-12-16
US20070004016A1 (en) 2007-01-04
US7125672B2 (en) 2006-10-24
WO2004101753A3 (en) 2006-02-02
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