BRPI0410681A - moléculas de ácido nucléico isoladas, polipeptìdio, gene quimérico, célula hospedeira transformada, métodos para a produção de ácido linoléico e de ácidos graxos w-3, método para modular a biosìntese de ácidos graxos w-3 ou w-6, óleo microbial, e métodos para a obtenção de uma molécula de ácido nucléico - Google Patents
moléculas de ácido nucléico isoladas, polipeptìdio, gene quimérico, célula hospedeira transformada, métodos para a produção de ácido linoléico e de ácidos graxos w-3, método para modular a biosìntese de ácidos graxos w-3 ou w-6, óleo microbial, e métodos para a obtenção de uma molécula de ácido nucléicoInfo
- Publication number
- BRPI0410681A BRPI0410681A BRPI0410681-4A BRPI0410681A BRPI0410681A BR PI0410681 A BRPI0410681 A BR PI0410681A BR PI0410681 A BRPI0410681 A BR PI0410681A BR PI0410681 A BRPI0410681 A BR PI0410681A
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- methods
- fatty
- desaturase
- obtaining
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract 3
- 229930195729 fatty acid Natural products 0.000 title abstract 3
- 239000000194 fatty acid Substances 0.000 title abstract 3
- 150000004665 fatty acids Chemical class 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 title abstract 2
- 235000020778 linoleic acid Nutrition 0.000 title abstract 2
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 title abstract 2
- 230000000813 microbial effect Effects 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 title abstract 2
- 230000015572 biosynthetic process Effects 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108010087894 Fatty acid desaturases Proteins 0.000 abstract 2
- 102000009114 Fatty acid desaturases Human genes 0.000 abstract 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 abstract 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 abstract 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 abstract 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 abstract 1
- 239000005642 Oleic acid Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"MOLéCULAS DE áCIDO NUCLéICO ISOLADAS, POLIPEPTìDIO, GENE QUIMéRICO, CéLULA HOSPEDEIRA TRANSFORMADA, MéTODOS PARA A PRODUçãO DE áCIDO LINOLéICO E DE áCIDOS GRAXOS <sym>-3, MéTODO PARA MODULAR A BIOSìNTESE DE áCIDOS GRAXOS <sym>-3 OU <sym>-6, óLEO MICROBIAL, E MéTODOS PARA A OBTENçãO DE UMA MOLéCULA DE áCIDO NUCLéICO". A presente invenção refere-se a uma <30>12 desaturase de ácido graxo com capacidade de catalisar a conversão de ácido oléico em ácido linoléico (LA; 18:2). São descritas as seqüências de ácido nucléico que codificam a desaturase, as seqüências de ácido nucléico que hibridizam na mesma, os construtos de DNA que compreendem o gene de desaturase, e os microorganismos hospedeiros recombinantes que expressam níveis aumentados da desaturase. Métodos para aumentar a produção de ácidos graxis <sym>-3 e/ou <sym>-6 específicos são descritos pela superexpressão da <30>12 desaturase de ácido graxo ou pelo rompimento do gene nativo.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US46867703P | 2003-05-07 | 2003-05-07 | |
| US48420903P | 2003-06-30 | 2003-06-30 | |
| PCT/US2004/014542 WO2004104167A2 (en) | 2003-05-07 | 2004-05-07 | A δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0410681A true BRPI0410681A (pt) | 2006-06-20 |
Family
ID=33479252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0410681-4A BRPI0410681A (pt) | 2003-05-07 | 2004-05-07 | moléculas de ácido nucléico isoladas, polipeptìdio, gene quimérico, célula hospedeira transformada, métodos para a produção de ácido linoléico e de ácidos graxos w-3, método para modular a biosìntese de ácidos graxos w-3 ou w-6, óleo microbial, e métodos para a obtenção de uma molécula de ácido nucléico |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US7214491B2 (pt) |
| EP (1) | EP1620561A4 (pt) |
| AU (1) | AU2004242095A1 (pt) |
| BR (1) | BRPI0410681A (pt) |
| CA (1) | CA2523786A1 (pt) |
| NO (1) | NO20055777L (pt) |
| WO (1) | WO2004104167A2 (pt) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7238482B2 (en) * | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US20070072275A1 (en) * | 2003-08-22 | 2007-03-29 | Misa Ochiai | Method for breeding lipid-producing fungus |
| BRPI0511236A (pt) * | 2004-06-04 | 2007-11-27 | Fluxome Sciences As | método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado |
| BRPI0512481A (pt) | 2004-06-25 | 2008-03-11 | Du Pont | polinucleotìdeo isolado, polipeptìdeo, construção recombinante, célula, yarrowia sp.transformada, métodos para transformar uma célula, produzir uma planta transformada, produzir levedura, produzir ácidos graxos poliinsaturados e produzir pelo menos um ácido graxo poliinsaturados, sementes, óleos, plantas de sementes oleaginosa e alimentos ou rações |
| US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| BRPI0609040B1 (pt) * | 2005-03-18 | 2018-07-31 | Microbia, Inc. | Fungo recombinante do gênero yarrowia, método de produção de um carotenóide e método de preparo de um aditivo alimentício ou ração contendo um carotenóide |
| US7943823B2 (en) | 2006-04-28 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| EP2078092A2 (en) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| US20110016581A1 (en) | 2006-10-23 | 2011-01-20 | E.I. Du Pont De Nemours And Company | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
| US7709239B2 (en) * | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
| US8846374B2 (en) * | 2006-12-12 | 2014-09-30 | E I Du Pont De Nemours And Company | Carotenoid production in a recombinant oleaginous yeast |
| ES2559312T3 (es) * | 2007-04-03 | 2016-02-11 | E. I. Du Pont De Nemours And Company | Multienzimas y su uso en la fabricación de ácidos grasos poliinsaturados |
| US7943365B2 (en) | 2007-05-03 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Δ-5 desaturases and their use in making polyunsaturated fatty acids |
| JP5711968B2 (ja) | 2007-10-03 | 2015-05-07 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ（ＹａｒｒｏｗｉａＬｉｐｏｌｙｔｉｃａ）の最適化株 |
| CA2701130A1 (en) * | 2007-10-03 | 2009-04-09 | E.I. Du Pont De Nemours And Company | Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms |
| US20090264520A1 (en) * | 2008-04-21 | 2009-10-22 | Asha Lipid Sciences, Inc. | Lipid-containing compositions and methods of use thereof |
| US7892792B2 (en) | 2008-06-27 | 2011-02-22 | Indian Institute Of Science | Cells expressing Pichia cytochrome C |
| US9394333B2 (en) | 2008-12-02 | 2016-07-19 | Wave Life Sciences Japan | Method for the synthesis of phosphorus atom modified nucleic acids |
| CA2744716C (en) | 2008-12-18 | 2017-05-23 | E.I. Du Pont De Nemours And Company | Reducing byproduction of malonates in a fermentation process |
| MX342945B (es) | 2009-07-06 | 2016-10-18 | Ontorii Inc * | Profármacos de ácido nucleico novedosos y métodos de uso de los mismos. |
| WO2011109548A2 (en) * | 2010-03-02 | 2011-09-09 | Massachusetts Institute Of Technology | Microbial engineering for the production of fatty acids and fatty acid derivatives |
| AU2011242843A1 (en) * | 2010-04-22 | 2012-09-27 | E. I. Du Pont De Nemours And Company | Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass |
| DK2620428T3 (da) | 2010-09-24 | 2019-07-01 | Wave Life Sciences Ltd | Asymmetrisk hjælpegruppe |
| US9133463B2 (en) | 2011-03-07 | 2015-09-15 | Dsm Nutritional Products Ag | Engineering microorganisms |
| US9605019B2 (en) | 2011-07-19 | 2017-03-28 | Wave Life Sciences Ltd. | Methods for the synthesis of functionalized nucleic acids |
| SG11201500243WA (en) | 2012-07-13 | 2015-04-29 | Shin Nippon Biomedical Lab Ltd | Chiral nucleic acid adjuvant |
| SG11201500239VA (en) | 2012-07-13 | 2015-03-30 | Wave Life Sciences Japan | Asymmetric auxiliary group |
| KR20240148947A (ko) | 2012-07-13 | 2024-10-11 | 웨이브 라이프 사이언시스 리미티드 | 키랄 제어 |
| CN102787128A (zh) * | 2012-08-28 | 2012-11-21 | 江南大学 | 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des |
| CA2892516A1 (en) | 2012-12-21 | 2014-06-26 | E. I. Du Pont De Nemours And Company | Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells |
| US9873880B2 (en) | 2013-03-13 | 2018-01-23 | Dsm Nutritional Products Ag | Engineering microorganisms |
| WO2015108048A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤 |
| EP3095460A4 (en) | 2014-01-15 | 2017-08-23 | Shin Nippon Biomedical Laboratories, Ltd. | Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent |
| WO2015108047A1 (ja) | 2014-01-15 | 2015-07-23 | 株式会社新日本科学 | 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤 |
| ES2917473T3 (es) | 2014-01-16 | 2022-07-08 | Wave Life Sciences Ltd | Diseño quiral |
| CN103834672B (zh) * | 2014-03-17 | 2015-12-09 | 昆明理工大学 | 一种δ12-脂肪酸脱氢酶基因及其应用 |
| CN110229832A (zh) * | 2019-06-16 | 2019-09-13 | 山东理工大学 | 一种提高耶式解脂酵母生物量和细胞壁产量的菌株和方法 |
| WO2025008447A1 (en) * | 2023-07-05 | 2025-01-09 | Bayer Aktiengesellschaft | Composition for use in agriculture |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ190194A (en) | 1978-05-08 | 1982-02-23 | Cpc International Inc | Production of oil by fermenting yeast in fatty acids |
| GB8507058D0 (en) | 1985-03-19 | 1985-04-24 | Efamol Ltd | Pharmaceutical & dietary compositions |
| GB8524276D0 (en) | 1985-10-02 | 1985-11-06 | Efamol Ltd | Pharmaceutical & dietary compositions |
| GB8524275D0 (en) | 1985-10-02 | 1985-11-06 | Efamol Ltd | Pharmaceutical & dietary compositions |
| IE63225B1 (en) | 1988-09-13 | 1995-04-05 | Efamol Holdings | Use of fatty acids in the treatment of myalgic encephalomyelitis |
| DE69103753T3 (de) | 1990-05-25 | 2008-01-24 | E.I. Dupont De Nemours And Co., Wilmington | Nukleotidsequenz des gens für stearoyl-acp-desaturase aus soja. |
| ZA92452B (en) * | 1991-01-24 | 1992-10-28 | Martek Corp | Microbial oil mixtures and uses thereof |
| DE69333025T2 (de) | 1992-11-17 | 2004-04-29 | E.I. Du Pont De Nemours And Co., Wilmington | Für mikrosom delta 12 fettsäuredesaturase kodierende gene und verwandte enzyme von pflanzen |
| US6372965B1 (en) | 1992-11-17 | 2002-04-16 | E.I. Du Pont De Nemours And Company | Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants |
| US6872872B1 (en) | 1992-11-17 | 2005-03-29 | E. I. Du Pont De Nemours And Company | Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants |
| US5968809A (en) | 1997-04-11 | 1999-10-19 | Abbot Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids |
| US6372966B1 (en) * | 2000-06-07 | 2002-04-16 | Pure Seed Testing Inc. | Kentucky bluegrass variety known as ‘Brilliant’ |
| US7211656B2 (en) * | 2002-01-30 | 2007-05-01 | Abbott Laboratories | Desaturase genes, enzymes encoded thereby, and uses thereof |
| MXPA04011613A (es) | 2002-05-22 | 2005-03-07 | Monsanto Technology Llc | Desaturasas de acido graso de hongos. |
| US7364883B2 (en) | 2004-05-07 | 2008-04-29 | Yeastern Biotech Co., Ltd. | Process for producing poly-unsaturated fatty acids by oleaginous yeasts |
-
2004
- 2004-05-06 US US10/840,325 patent/US7214491B2/en not_active Expired - Lifetime
- 2004-05-07 WO PCT/US2004/014542 patent/WO2004104167A2/en not_active Ceased
- 2004-05-07 BR BRPI0410681-4A patent/BRPI0410681A/pt not_active Application Discontinuation
- 2004-05-07 EP EP04751770A patent/EP1620561A4/en not_active Withdrawn
- 2004-05-07 AU AU2004242095A patent/AU2004242095A1/en not_active Abandoned
- 2004-05-07 CA CA002523786A patent/CA2523786A1/en not_active Abandoned
-
2005
- 2005-12-06 NO NO20055777A patent/NO20055777L/no not_active Application Discontinuation
-
2006
- 2006-07-27 US US11/494,318 patent/US7749703B2/en not_active Expired - Fee Related
- 2006-07-27 US US11/494,317 patent/US20060263866A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| NO20055777L (no) | 2006-02-07 |
| WO2004104167A3 (en) | 2005-04-28 |
| US20060263867A1 (en) | 2006-11-23 |
| US20050043527A1 (en) | 2005-02-24 |
| CA2523786A1 (en) | 2004-12-02 |
| US7214491B2 (en) | 2007-05-08 |
| EP1620561A2 (en) | 2006-02-01 |
| US7749703B2 (en) | 2010-07-06 |
| EP1620561A4 (en) | 2006-08-23 |
| NO20055777D0 (no) | 2005-12-06 |
| AU2004242095A1 (en) | 2004-12-02 |
| US20060263866A1 (en) | 2006-11-23 |
| WO2004104167A2 (en) | 2004-12-02 |
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