BRPI0411236A - método para a produção de células nervosas retinais a partir das células-tronco neurais/progenitoras derivadas de tecido de ìris e células nervosas retinais obtidas do mesmo método - Google Patents

método para a produção de células nervosas retinais a partir das células-tronco neurais/progenitoras derivadas de tecido de ìris e células nervosas retinais obtidas do mesmo método

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Publication number
BRPI0411236A
BRPI0411236A BRPI0411236-9A BRPI0411236A BRPI0411236A BR PI0411236 A BRPI0411236 A BR PI0411236A BR PI0411236 A BRPI0411236 A BR PI0411236A BR PI0411236 A BRPI0411236 A BR PI0411236A
Authority
BR
Brazil
Prior art keywords
retinal nerve
cells
nerve cells
pigmented epithelial
iris pigmented
Prior art date
Application number
BRPI0411236-9A
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English (en)
Inventor
Mitsuko Kosaka
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Japan Science & Tech Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science & Tech Agency filed Critical Japan Science & Tech Agency
Publication of BRPI0411236A publication Critical patent/BRPI0411236A/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2502/00—Coculture with; Conditioned medium produced by
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/08—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the nervous system
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Neurosurgery (AREA)
  • Acoustics & Sound (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Ophthalmology & Optometry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)

Abstract

"MéTODO PARA A PRODUçãO DE CéLULAS NERVOSAS RETINAIS A PARTIR DAS CéLULAS-TRONCO NEURAIS/PROGENITORAS DERIVADAS DE TECIDO DE ìRIS E CéLULAS NERVOSAS RETINAIS OBTIDAS DO MESMO MéTODO". A presente invenção refere-se a um método para a produção de células nervosas retinais por indução de diferenciação de células epiteliais pigmentadas da íris para formar as células nervosas retinais. Em um primeiro método, células epiteliais pigmentadas da íris derivadas de um mamífero e células-tronco retinais de embrião derivadas de um pássaro são co-cultivadas. Em um segundo método, células epiteliais pigmentadas da íris de um pássaro ou um mamífero são isoladas e as células epiteliais pigmentadas da íris são submetidas a cultivo aderente. De acordo com estes métodos, as células nervosas retinais podem ser produzidas por uso de células epiteliais pigmentadas da íris coletadas a partir de um paciente por si. Portanto, há uma possibilidade que tratamento médico regenerativo altamente eficaz pode ser realizado.
BRPI0411236-9A 2003-06-11 2004-06-11 método para a produção de células nervosas retinais a partir das células-tronco neurais/progenitoras derivadas de tecido de ìris e células nervosas retinais obtidas do mesmo método BRPI0411236A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003166646 2003-06-11
PCT/JP2004/008222 WO2004111213A1 (ja) 2003-06-11 2004-06-11 虹彩組織由来の神経幹細胞から網膜神経細胞を生産する方法、および、その方法により得られる網膜神経細胞

Publications (1)

Publication Number Publication Date
BRPI0411236A true BRPI0411236A (pt) 2006-07-11

Family

ID=33549263

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0411236-9A BRPI0411236A (pt) 2003-06-11 2004-06-11 método para a produção de células nervosas retinais a partir das células-tronco neurais/progenitoras derivadas de tecido de ìris e células nervosas retinais obtidas do mesmo método

Country Status (12)

Country Link
US (1) US8569056B2 (pt)
EP (2) EP1988160B1 (pt)
JP (2) JP4389087B2 (pt)
KR (1) KR100734714B1 (pt)
CN (2) CN1795266B (pt)
AT (2) ATE478136T1 (pt)
AU (1) AU2004248013B2 (pt)
BR (1) BRPI0411236A (pt)
CA (2) CA2528426C (pt)
DE (2) DE602004028768D1 (pt)
IL (1) IL172499A (pt)
WO (1) WO2004111213A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2484308A1 (en) * 2006-07-24 2012-08-08 International Stem Cell Corporation Synthetic cornea from retinal stem cells
JP2013135640A (ja) * 2011-12-28 2013-07-11 Shiseido Co Ltd 神経幹細胞から神経細胞及び／又はグリア細胞を形成する培養方法
JP6050033B2 (ja) * 2012-06-05 2016-12-21 日本メナード化粧品株式会社 幹細胞から外胚葉系細胞への分化誘導剤
US9597227B2 (en) * 2013-03-15 2017-03-21 Abbott Medical Optics Inc. Trans-sclera portal for delivery of therapeutic agents
EP2796545A1 (en) * 2013-04-26 2014-10-29 Université Pierre et Marie Curie (Paris 6) Methods for obtaining retinal progenitors, retinal pigmented epithelial cells and neural retinal cells
CN103409363B (zh) * 2013-08-06 2015-01-21 中国人民解放军总医院 感光前体细胞与视网膜组织体外共培养方法
CN104789521B (zh) * 2014-01-22 2017-11-07 广州康睿生物医药科技股份有限公司 一种晶状体上皮干细胞的分离培养方法
KR101895648B1 (ko) 2014-11-06 2018-09-05 단국대학교 천안캠퍼스 산학협력단 치수줄기세포를 포함하는 신경질환 치료용 약학 조성물 및 이의 제조방법
CN113943707B (zh) * 2021-09-24 2023-11-21 中国人民解放军陆军军医大学第一附属医院 一种视网膜前体细胞的筛选和培养方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001234998B2 (en) 2000-02-11 2006-06-08 Childrens Hospital Of Orange County A California Corporation Isolation and transplantation of retinal stem cells
JP2002112764A (ja) 2000-10-05 2002-04-16 Tohoku Techno Arch Co Ltd 株化網膜神経細胞
JP2002325571A (ja) 2001-04-27 2002-11-12 Purotekku:Kk 網膜の分化誘導方法
JP3723152B2 (ja) 2002-05-10 2005-12-07 独立行政法人科学技術振興機構 哺乳動物の虹彩色素上皮細胞由来の神経幹細胞の生産方法、および該神経幹細胞から神経系細胞を生産する方法

Also Published As

Publication number Publication date
EP1988160A1 (en) 2008-11-05
EP1988160B1 (en) 2010-08-18
JP2009273473A (ja) 2009-11-26
CA2717252A1 (en) 2004-12-23
ATE478136T1 (de) 2010-09-15
CN1795266B (zh) 2010-08-18
WO2004111213A1 (ja) 2004-12-23
CA2528426A1 (en) 2004-12-23
ATE451449T1 (de) 2009-12-15
CN101186900A (zh) 2008-05-28
EP1640450A4 (en) 2006-09-06
IL172499A0 (en) 2006-04-10
EP1640450B1 (en) 2009-12-09
CN1795266A (zh) 2006-06-28
US20060134280A1 (en) 2006-06-22
CA2717252C (en) 2013-09-24
AU2004248013A1 (en) 2004-12-23
DE602004024529D1 (de) 2010-01-21
IL172499A (en) 2011-09-27
JPWO2004111213A1 (ja) 2006-07-27
JP4389087B2 (ja) 2009-12-24
CN101186900B (zh) 2010-09-15
CA2528426C (en) 2012-04-03
EP1640450A1 (en) 2006-03-29
KR100734714B1 (ko) 2007-07-02
KR20060017543A (ko) 2006-02-23
DE602004028768D1 (de) 2010-09-30
US8569056B2 (en) 2013-10-29
AU2004248013B2 (en) 2007-02-08

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 11A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2310 DE 14-04-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.

B15K Others concerning applications: alteration of classification

Ipc: C12N 5/0793 (2010.01), C12N 5/079 (2010.01)