BRPI0412813A - determinação da seqüência de um polinucleotìdeo - Google Patents

determinação da seqüência de um polinucleotìdeo

Info

Publication number
BRPI0412813A
BRPI0412813A BRPI0412813-3A BRPI0412813A BRPI0412813A BR PI0412813 A BRPI0412813 A BR PI0412813A BR PI0412813 A BRPI0412813 A BR PI0412813A BR PI0412813 A BRPI0412813 A BR PI0412813A
Authority
BR
Brazil
Prior art keywords
target polynucleotide
polymerase
polynucleotide
sequence
sequence determination
Prior art date
Application number
BRPI0412813-3A
Other languages
English (en)
Inventor
Daniel Henry Densham
Original Assignee
Medical Biosystems Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Biosystems Ltd filed Critical Medical Biosystems Ltd
Publication of BRPI0412813A publication Critical patent/BRPI0412813A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/113—Time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

"DETERMINAçãO DE SEQUêNCIA DE UM POLINUCLEOTìDEO". A seqüência de um polinucleotídeo alvo pode ser determinada por: (i) contato do polinucleotídeo alvo com uma enzima de polimerase e um dos nucleotídeos A, T(U), G e C sob condições adequadas para que a reação de polimerase tenha prosseguimento; (ii) medição do tempo que leva para a polimerase se unir a e subseqüentemente dissociar do polinucleotídeo alvo, para dessa determinar se a polimerase incorporou o nucleotídeo ao polinucleotídeo alvo; (iii) repetição opcional das etapas (i) e (ii) com nucleotídeos adicionais, para identificar dessa forma a seqüência do polinucleotídeo alvo.
BRPI0412813-3A 2003-07-24 2004-07-26 determinação da seqüência de um polinucleotìdeo BRPI0412813A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0317343.2A GB0317343D0 (en) 2003-07-24 2003-07-24 Polynucleotide sequencing
PCT/GB2004/003232 WO2005010210A2 (en) 2003-07-24 2004-07-26 Determining the sequence of a polynucleotide

Publications (1)

Publication Number Publication Date
BRPI0412813A true BRPI0412813A (pt) 2006-09-26

Family

ID=27772590

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0412813-3A BRPI0412813A (pt) 2003-07-24 2004-07-26 determinação da seqüência de um polinucleotìdeo

Country Status (21)

Country Link
US (3) US7604963B2 (pt)
EP (1) EP1649051B1 (pt)
JP (1) JP4394684B2 (pt)
KR (1) KR20060052863A (pt)
CN (1) CN1852991A (pt)
AT (1) ATE388243T1 (pt)
AU (1) AU2004259893A1 (pt)
BR (1) BRPI0412813A (pt)
CA (2) CA2780958C (pt)
DE (1) DE602004012273T2 (pt)
DK (1) DK1649051T3 (pt)
ES (1) ES2303083T3 (pt)
GB (1) GB0317343D0 (pt)
IL (1) IL173314A0 (pt)
IS (1) IS8314A (pt)
MX (1) MXPA06000962A (pt)
PL (1) PL1649051T3 (pt)
PT (1) PT1649051E (pt)
RU (1) RU2006105626A (pt)
SI (1) SI1649051T1 (pt)
WO (1) WO2005010210A2 (pt)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001511358A (ja) * 1997-07-28 2001-08-14 メディカル・バイオシステムズ・リミテッド 核酸配列の解析
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB0112238D0 (en) * 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
JP2004347621A (ja) * 2003-04-24 2004-12-09 Dainippon Printing Co Ltd 透過型スクリーン
JP4431549B2 (ja) 2006-05-31 2010-03-17 株式会社日立ハイテクノロジーズ 蛍光分析装置
GB0721340D0 (en) * 2007-10-30 2007-12-12 Isis Innovation Polymerase-based single-molecule DNA sequencing
JP4982523B2 (ja) * 2009-05-11 2012-07-25 株式会社日立ハイテクノロジーズ 蛍光分析方法，蛍光分析装置及び画像検出方法
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
WO2013096692A1 (en) 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
US9593371B2 (en) * 2013-12-27 2017-03-14 Intel Corporation Integrated photonic electronic sensor arrays for nucleic acid sequencing
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
EP3458082A4 (en) * 2016-04-04 2020-07-29 The Trustees of Columbia University in the City of New York SYNTHESIS DNA SEQUENCING WITH NUCLEOTID ANALOGUES AND RAMAN DETECTION
EP3449012B1 (en) 2016-04-29 2020-12-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP3497236B1 (en) 2016-08-15 2021-11-03 Omniome, Inc. Method for sequencing nucleic acids
EP3562962B1 (en) 2016-12-30 2022-01-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
CN110249059B (zh) 2017-01-20 2021-04-13 欧姆尼欧美公司 核酸的等位基因特异性捕获
US9932631B1 (en) 2017-09-11 2018-04-03 Omniome, Inc. Genotyping by polymerase binding
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
CN119264211A (zh) 2018-08-27 2025-01-07 瑞泽恩制药公司 拉曼光谱在下游纯化中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6416952B1 (en) * 1989-06-07 2002-07-09 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP2001511358A (ja) * 1997-07-28 2001-08-14 メディカル・バイオシステムズ・リミテッド 核酸配列の解析
CN1245218A (zh) 1998-08-19 2000-02-23 中国人民解放军军事医学科学院放射医学研究所 一种固相逐个碱基核酸分析方法和仪器
WO2000053805A1 (en) * 1999-03-10 2000-09-14 Asm Scientific, Inc. A method for direct nucleic acid sequencing
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6908736B1 (en) * 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB0112238D0 (en) * 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
GB0413082D0 (en) * 2004-06-11 2004-07-14 Medical Biosystems Ltd Method

Also Published As

Publication number Publication date
PL1649051T3 (pl) 2008-12-31
GB0317343D0 (en) 2003-08-27
US7604963B2 (en) 2009-10-20
US7888073B2 (en) 2011-02-15
CN1852991A (zh) 2006-10-25
AU2004259893A1 (en) 2005-02-03
US20080070236A1 (en) 2008-03-20
SI1649051T1 (sl) 2008-10-31
US8592183B2 (en) 2013-11-26
ATE388243T1 (de) 2008-03-15
WO2005010210A2 (en) 2005-02-03
DE602004012273D1 (de) 2008-04-17
IS8314A (is) 2006-02-20
US20100279288A1 (en) 2010-11-04
CA2533579C (en) 2012-09-11
JP2006528485A (ja) 2006-12-21
EP1649051B1 (en) 2008-03-05
CA2780958C (en) 2016-10-25
DE602004012273T2 (de) 2009-04-30
PT1649051E (pt) 2008-05-26
RU2006105626A (ru) 2006-08-10
JP4394684B2 (ja) 2010-01-06
CA2780958A1 (en) 2005-02-03
EP1649051A2 (en) 2006-04-26
ES2303083T3 (es) 2008-08-01
CA2533579A1 (en) 2005-02-03
IL173314A0 (en) 2006-06-11
US20110097729A1 (en) 2011-04-28
MXPA06000962A (es) 2006-03-30
DK1649051T3 (da) 2008-07-07
KR20060052863A (ko) 2006-05-19
WO2005010210A3 (en) 2005-03-24

Similar Documents

Publication Publication Date Title
BRPI0412813A (pt) determinação da seqüência de um polinucleotìdeo
WO2009102878A3 (en) Method for archiving and clonal expansion
DK1778867T3 (da) Fremgangsmåder og sammensætninger til detektering af nucleinsyrer i en biologisk prøve
SE0301951D0 (sv) New method
RU2017100015A (ru) Набор или устройство для обнаружения рака желчных путей и способ обнаружения
EP4450642A3 (en) Single molecule nucleic acid sequence analysis processes and compositions
RU2017101174A (ru) Набор или устройство для обнаружения рака печени и способ обнаружения
WO2009097368A3 (en) Methods and compositions for efficient base calling in sequencing reactions
NZ629701A (en) Target detection and signal amplification
WO2010062913A3 (en) Methods and systems for analysis of sequencing data
WO2004072238A3 (en) Terminal-phosphate-labeled nucleotides with new linkers
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
WO2008076842A3 (en) Sequencing methods
MX2007009809A (es) Amplificacion de sonda por seleccion.
AU2009355009A8 (en) THD primer target detection
RU2016116333A (ru) Определение нуклеиновых кислот путем амплификации, основанной на встраивании в цепь
WO2011156387A3 (en) Enumeration of nucleic acids
RU2017102991A (ru) Композиции и способы обнаружения и анализа микробактерии туберкулеза (mycobacterium tuderculosis)
WO2005054270A3 (en) Identification and design of sirna molecules
JP2017521086A5 (pt)
WO2004048397A3 (en) Detectable labeled nucleoside analogs and methods of use thereof
CN103740824B (zh) 一种两核苷酸实时合成测序图谱鉴定微生物种群的方法
EP2383349A3 (en) Primer and probe for detection of mycobacterium avium and method for detection of mycobacterium avium using the same
ATE494392T1 (de) Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
TW200506067A (en) Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A E 7A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2112 DE 28/06/2011.