BRPI0416360A - processo para o cultivo de microorganismos da espécie thraustochytriales com a utilização de um meio de baixo número otimizado - Google Patents

processo para o cultivo de microorganismos da espécie thraustochytriales com a utilização de um meio de baixo número otimizado

Info

Publication number
BRPI0416360A
BRPI0416360A BRPI0416360-5A BRPI0416360A BRPI0416360A BR PI0416360 A BRPI0416360 A BR PI0416360A BR PI0416360 A BRPI0416360 A BR PI0416360A BR PI0416360 A BRPI0416360 A BR PI0416360A
Authority
BR
Brazil
Prior art keywords
medium
microorganisms
salts
low
optimized
Prior art date
Application number
BRPI0416360-5A
Other languages
English (en)
Inventor
Matthias Ruesing
Markus Luy
Original Assignee
Nutrinova Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nutrinova Gmbh filed Critical Nutrinova Gmbh
Publication of BRPI0416360A publication Critical patent/BRPI0416360A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Edible Oils And Fats (AREA)

Abstract

PROCESSO PARA O CULTIVO DE MICROORGANISMOS DA ESPéCIE THRAUSTOCHYTRIALES COM A UTILIZAçãO DE UM MEIO DE BAIXO NúMERO OTIMIZADO. A presente invenção refere-se a um processo otimizado para o cultivo de microorganismos do gênero Thraustochytriales, no qual os microorganismos são cultivados em um meio de baixa salinidade sem adição de sais de sódio e sais de cloreto, com um teor de sal total de menos do que 3,5 g/l de sais totais (correspondentemente menos do que 10 % do teor de sal de água marinha) e isolamento subseqüente dos PUFAa dos microorganismos e/ou do meio. Especialmente são descritos novos meio otimizados com teor de sal total consideravelmente reduzido com teor de NaCI particularmente reduzido. Uma nova combinação de diversos sais como composição do meio não mais de 1,75 g/l de íons da Na¬ +¬ e CI¬ -¬ na soma das partes em peso, permite aperfeiçoar competentemente e simplificar consideravelmente a produção dos PUFAs. Além disso, o meio descrito é preferentemente inteiramente isento de aditivos de sais de sódio e cloreto, o que ajuda a evitar danos ambientais das águas residuais salinas.
BRPI0416360-5A 2003-11-10 2004-11-10 processo para o cultivo de microorganismos da espécie thraustochytriales com a utilização de um meio de baixo número otimizado BRPI0416360A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2003152838 DE10352838A1 (de) 2003-11-10 2003-11-10 Verfahren zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales unter Verwendung eines optimierten Niedrigsalzmediums
PCT/EP2004/012718 WO2005045003A2 (de) 2003-11-10 2004-11-10 Verfahren zur kultivierung von mikroorganismen der gattung thraustochytriales unter verwendung eines optimierten niedrigsalzmediums

Publications (1)

Publication Number Publication Date
BRPI0416360A true BRPI0416360A (pt) 2007-05-08

Family

ID=34559600

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0416360-5A BRPI0416360A (pt) 2003-11-10 2004-11-10 processo para o cultivo de microorganismos da espécie thraustochytriales com a utilização de um meio de baixo número otimizado

Country Status (14)

Country Link
US (2) US8900831B2 (pt)
EP (2) EP1685241A2 (pt)
JP (1) JP4791367B2 (pt)
KR (1) KR101238613B1 (pt)
CN (2) CN1977038B (pt)
AU (1) AU2004287954B2 (pt)
BR (1) BRPI0416360A (pt)
CA (1) CA2545410A1 (pt)
DE (1) DE10352838A1 (pt)
EA (1) EA011858B1 (pt)
IL (1) IL175520A (pt)
SG (1) SG148157A1 (pt)
WO (1) WO2005045003A2 (pt)
ZA (1) ZA200603854B (pt)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2084290B1 (en) * 2006-10-27 2012-05-02 Lonza AG Production of omega-3 fatty acids in microflora of thraustochytriales using modified media
EP2071019A1 (en) * 2007-12-15 2009-06-17 Lonza AG Method for the cultivation of microoranisms of the order thraustochytriales
EP2105506A1 (de) * 2008-03-26 2009-09-30 Lonza Ag Verfahren zur Herstellung PUFAs enthaltender Öle unter Verwendung von Mikroorganismen der Ordnung Labyrinthulomycota
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
CA2993690C (en) 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
MX342216B (es) 2011-07-21 2016-09-20 Dsm Ip Assets Bv Composiciones de acidos grasos.
KR101134324B1 (ko) * 2011-10-07 2012-04-13 나윤경 미생물 대량 생산을 위한 분말 배지
KR101418066B1 (ko) * 2012-11-30 2014-07-09 롯데케미칼 주식회사 미세조류를 이용한 도코사헥사엔산 생산방법
EP2826384A1 (de) 2013-07-16 2015-01-21 Evonik Industries AG Verfahren zur Trocknung von Biomasse
FR3019559B1 (fr) * 2014-04-03 2018-01-05 Fermentalg Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture a teneur reduite en sodium et en chlorure pour la production de dha
MA39445B2 (fr) * 2014-05-08 2020-10-28 Donald M Smith Sélection, production et alimentation d'algues entières comme complément alimentaire pour le bétail et les bisons pour produire de la viande à teneur élevée en oméga 3's pour la santé humaine
KR102236782B1 (ko) 2014-05-22 2021-04-05 마라 리뉴어블즈 코퍼레이션 미생물에서 오일 생산 방법
CN104004661B (zh) * 2014-05-22 2017-12-15 广东省生物工程研究所（广州甘蔗糖业研究所） 一种可提高鸡蛋品质的蛋鸡日粮
CN104277975A (zh) * 2014-09-28 2015-01-14 江苏科技大学 脱脂蚕蛹渣用于产油微生物培养的预处理方法及其产物和应用
CA2958439C (en) 2014-10-02 2022-09-20 Evonik Industries Ag Feedstuff of high abrasion resistance and good stability in water, containing pufas
BR112017005388B1 (pt) 2014-10-02 2022-09-13 Evonik Operations Gmbh Alimento para animais contendo biomassa de aurantiochytrium
CN106793803B (zh) 2014-10-02 2021-03-09 赢创运营有限公司 通过挤压含pufa的生物质来制备含pufa的饲料的方法
CN107075540A (zh) 2014-10-02 2017-08-18 赢创德固赛有限公司 用于制备具有高细胞稳定性的含pufa生物质的方法
KR102405390B1 (ko) 2016-07-13 2022-06-03 에보닉 오퍼레이션스 게엠베하 용해된 지질을 함유하는 바이오매스로부터 지질을 분리하는 방법
KR102027632B1 (ko) * 2016-12-06 2019-10-01 국립해양생물자원관 고온성 미생물 배양용 고체배지조성물 및 그 제조방법
ES2872009T3 (es) 2016-12-27 2021-11-02 Evonik Degussa Gmbh Método de aislamiento de lípidos a partir de una biomasa que contiene lípidos
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
WO2019219443A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica
WO2019219396A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion
CN114525312A (zh) * 2020-11-06 2022-05-24 嘉必优生物技术（武汉）股份有限公司 一种使用裂殖壶菌发酵生产dha的方法
SE2151489A1 (en) * 2021-12-07 2023-06-08 Mycorena Ab A food product or food ingredient comprising fungi biomass with an increased intracellular fat content
CN118389397A (zh) * 2024-06-26 2024-07-26 暨南大学 耐受低盐度的寇氏隐甲藻的制备方法、碎米水解液的应用及dha的生产方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6451567B1 (en) 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
US5407957A (en) 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
US6410281B1 (en) 1992-07-10 2002-06-25 Omegatech, Inc. Reducing corrosion in a fermentor by providing sodium with a non-chloride sodium salt
JP2764572B2 (ja) * 1995-04-17 1998-06-11 工業技術院長 ドコサヘキサエン酸生産能を有する新規微生物及びそれを用いたドコサヘキサエン酸の製造方法
WO1996033263A1 (en) 1995-04-17 1996-10-24 JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
BR9710394A (pt) * 1996-07-23 2000-01-11 Nagase Biochemicals Ltda Processo para preparação de ácido docosahexaenóico e ácido docosapentaenóico;processo de preparação a partir de lipidio;microorganismo produtor de lipìdios;alimentos e congenêres abtidos através do processo
CN1187371C (zh) * 2003-03-19 2005-02-02 中国科学院南海海洋研究所 海水藻蓝蛋白的制备方法

Also Published As

Publication number Publication date
IL175520A (en) 2014-08-31
WO2005045003A3 (de) 2005-08-04
SG148157A1 (en) 2008-12-31
ZA200603854B (en) 2007-11-28
EA011858B1 (ru) 2009-06-30
US20150104557A1 (en) 2015-04-16
IL175520A0 (en) 2006-09-05
JP2007510422A (ja) 2007-04-26
AU2004287954A1 (en) 2005-05-19
EA200600947A1 (ru) 2007-02-27
CN1977038A (zh) 2007-06-06
CN104073442A (zh) 2014-10-01
WO2005045003A2 (de) 2005-05-19
DE10352838A1 (de) 2005-07-07
KR101238613B1 (ko) 2013-02-28
US20070054384A1 (en) 2007-03-08
EP2816104A1 (de) 2014-12-24
KR20070042115A (ko) 2007-04-20
EP1685241A2 (de) 2006-08-02
CA2545410A1 (en) 2005-05-19
JP4791367B2 (ja) 2011-10-12
CN1977038B (zh) 2014-12-24
US8900831B2 (en) 2014-12-02
AU2004287954B2 (en) 2010-04-29

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 1/10 , C12P 7/64 , A23K 1/16 , C12N 1/12 , C12N 1/14

Ipc: C12N 1/10 (2006.01), C12P 7/64 (2006.01), C12N 1/1

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 24, 8O C/C 13, 10 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]