BRPI0418641A - organização similar a tecido de células e construtos similares a tecidos macroscópicos, gerados por cultura de macromassa de células, e método de cultura de macromassa - Google Patents
organização similar a tecido de células e construtos similares a tecidos macroscópicos, gerados por cultura de macromassa de células, e método de cultura de macromassaInfo
- Publication number
- BRPI0418641A BRPI0418641A BRPI0418641-9A BRPI0418641A BRPI0418641A BR PI0418641 A BRPI0418641 A BR PI0418641A BR PI0418641 A BRPI0418641 A BR PI0418641A BR PI0418641 A BRPI0418641 A BR PI0418641A
- Authority
- BR
- Brazil
- Prior art keywords
- tissue
- cells
- culture
- organization
- macroscopic
- Prior art date
Links
- 230000008520 organization Effects 0.000 title abstract 5
- 238000012136 culture method Methods 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 6
- 238000010899 nucleation Methods 0.000 abstract 4
- 230000009772 tissue formation Effects 0.000 abstract 3
- 230000001939 inductive effect Effects 0.000 abstract 2
- 210000004504 adult stem cell Anatomy 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 210000001612 chondrocyte Anatomy 0.000 abstract 1
- 230000002500 effect on skin Effects 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 230000003328 fibroblastic effect Effects 0.000 abstract 1
- 239000003102 growth factor Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 210000000963 osteoblast Anatomy 0.000 abstract 1
- 210000005009 osteogenic cell Anatomy 0.000 abstract 1
- 210000002536 stromal cell Anatomy 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Prostheses (AREA)
Abstract
ORGANIZAçãO SIMILAR A TECIDO DE CéLULAS E CONSTRUTOS SIMILARES A TECIDOS MACROSCóPICOS, GERADOS POR CULTURA DE MACROMASSA DE CéLULAS, E MéTODO DE CULTURA DE MACROMASSA" Trata-se de uma organização similar a tecido tridimensional de células pela cultura de elevada densidade de semeadura de células denominada como cultura de macromassa. Através da cultura de macromassa, as células podem ser levadas a se organizar em uma forma similar a tecido sem a ajuda de uma estrutura de suporte, e construtos similares a tecidos macroscópicos tridimensionais podem ser obtidos integralmente a partir das células. A organização similar a tecido e os construtos parecidos com tecidos macroscópicos podem ser gerados a partir de células fibroblásticas de origem mesenquimal (pelo menos), as quais podem ser tanto células diferenciadas quanto células tronco adultas multipotentes. Neste trabalho, a organização similar a tecido e os construtos similares a tecidos macroscópicos foram gerados a partir de fibroblastos dermais, células osteogênicas derivadas de células estromais de adipose, condrócitos, e de osteoblastos. O fator que causa a formação de tecido macroscópico é a cultura em larga escala a uma elevada densidade de semeadura de células por unidade de área ou espaço tridimensional, isto é, a cultura de macromassa obtida em uma larga escala. Nenhuma estrutura de suporte ou matriz estranha é utilizada para a geração dos tecidos, os tecidos são de origem completamente celular. Nenhum outro agente (exceto a elevad densidade de semeadura de células) que ajuda na formação dos tecidos, tais como produtos químicos de indução de tecido, fatores do crescimento de indução de tecido, substrato com propriedades especiais, cultura rotativa, etc., é empregado para a formação dos tecidos. Essas massas similares a tecidos podem ser potencialmente utilizadas como substituições de tecido no corpo humano. A organização similar a tecido pela cultura de macromassa de elevada densidade de semeadura de células também podem ser gerada ao nível microscópico.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IN2004/000086 WO2005095585A1 (en) | 2004-03-31 | 2004-03-31 | Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells, and the method of macromass culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI0418641A true BRPI0418641A (pt) | 2007-08-07 |
| BRPI0418641C1 BRPI0418641C1 (pt) | 2009-11-24 |
Family
ID=34958569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRC10418641-9A BRPI0418641C1 (pt) | 2004-03-31 | 2004-03-31 | equivalente tecidual tridimensional usando cultura de macromassa |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1730264B1 (pt) |
| CN (1) | CN1938419B (pt) |
| AT (1) | ATE447609T1 (pt) |
| AU (1) | AU2004317828B2 (pt) |
| BR (1) | BRPI0418641C1 (pt) |
| CA (1) | CA2566374A1 (pt) |
| DE (1) | DE602004023974D1 (pt) |
| IL (2) | IL178265A (pt) |
| WO (1) | WO2005095585A1 (pt) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7993922B2 (en) | 2002-10-18 | 2011-08-09 | Reliance Life Sciences Pvt. Ltd. | Three-dimensional tissue equivalent using macromass culture |
| ES2641528T3 (es) * | 2006-06-27 | 2017-11-10 | Shiseido Company, Limited | Masa celular capaz de servir como estructura de tipo órgano primitivo compuesta por una diversidad de tipos de células de origen somático |
| WO2008068776A2 (en) | 2006-12-08 | 2008-06-12 | Reliance Life Sciences Pvt. Ltd. | Three-dimensional tissue equivalent using macromass culture. |
| EP2138571B1 (en) | 2008-06-26 | 2017-04-12 | SpheroTec GmbH | Process for the preparation of multicellular spheroids |
| KR101733137B1 (ko) * | 2015-12-30 | 2017-05-08 | (주)엑셀세라퓨틱스 | 연골조직 제조를 위한 3차원 오가노이드 블록 제작 방법 |
| CN106754669B (zh) * | 2016-11-23 | 2020-04-17 | 河海大学常州校区 | 基于反应-扩散模型的多细胞结构的制备方法及制备系统 |
| EP3417888A1 (de) | 2017-06-25 | 2018-12-26 | co.don AG | Verfahren zum herstellen von transplantierbarem knorpelgewebe |
| IL306070A (en) | 2018-05-03 | 2023-11-01 | Collplant Ltd | Dermal fillers and their uses |
| CN110157617B (zh) * | 2019-04-29 | 2021-07-16 | 重庆大学附属肿瘤医院 | 宫颈癌细胞三维培养装置及其应用 |
| US12435312B2 (en) | 2022-02-28 | 2025-10-07 | Brown University | Quantifying cell-derived changes in collagen synthesis, alignment, and mechanics in a 3D connective tissue model |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6197586B1 (en) * | 1997-12-12 | 2001-03-06 | The Regents Of The University Of California | Chondrocyte-like cells useful for tissue engineering and methods |
| JP2002529073A (ja) * | 1998-11-12 | 2002-09-10 | セル サイエンス セラピューティックス インコーポレーテッド | 三次元装置における造血前駆細胞からのリンパ様組織特異的細胞の生成 |
| US6429013B1 (en) * | 1999-08-19 | 2002-08-06 | Artecel Science, Inc. | Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair |
-
2004
- 2004-03-31 EP EP04770637A patent/EP1730264B1/en not_active Expired - Lifetime
- 2004-03-31 AU AU2004317828A patent/AU2004317828B2/en not_active Ceased
- 2004-03-31 CN CN2004800426456A patent/CN1938419B/zh not_active Expired - Fee Related
- 2004-03-31 WO PCT/IN2004/000086 patent/WO2005095585A1/en not_active Ceased
- 2004-03-31 BR BRC10418641-9A patent/BRPI0418641C1/pt not_active IP Right Cessation
- 2004-03-31 AT AT04770637T patent/ATE447609T1/de not_active IP Right Cessation
- 2004-03-31 DE DE602004023974T patent/DE602004023974D1/de not_active Expired - Lifetime
- 2004-03-31 CA CA002566374A patent/CA2566374A1/en not_active Abandoned
-
2006
- 2006-09-21 IL IL178265A patent/IL178265A/en not_active IP Right Cessation
-
2007
- 2007-11-18 IL IL187448A patent/IL187448A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| IL187448A0 (en) | 2008-02-09 |
| CN1938419A (zh) | 2007-03-28 |
| CA2566374A1 (en) | 2005-10-13 |
| CN1938419B (zh) | 2011-12-14 |
| AU2004317828A1 (en) | 2005-10-13 |
| WO2005095585A1 (en) | 2005-10-13 |
| HK1092507A1 (en) | 2007-02-09 |
| EP1730264A1 (en) | 2006-12-13 |
| ATE447609T1 (de) | 2009-11-15 |
| DE602004023974D1 (de) | 2009-12-17 |
| EP1730264B1 (en) | 2009-11-04 |
| AU2004317828B2 (en) | 2010-06-24 |
| IL187448A (en) | 2012-05-31 |
| IL178265A0 (en) | 2006-12-31 |
| BRPI0418641C1 (pt) | 2009-11-24 |
| IL178265A (en) | 2010-11-30 |
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