BRPI0509846A - sistema de expressão para a preparação de proteìnas de fusão de il-15/fc e seu uso - Google Patents

sistema de expressão para a preparação de proteìnas de fusão de il-15/fc e seu uso

Info

Publication number
BRPI0509846A
BRPI0509846A BRPI0509846-7A BRPI0509846A BRPI0509846A BR PI0509846 A BRPI0509846 A BR PI0509846A BR PI0509846 A BRPI0509846 A BR PI0509846A BR PI0509846 A BRPI0509846 A BR PI0509846A
Authority
BR
Brazil
Prior art keywords
expression system
nucleic acid
preparation
fusion proteins
expression
Prior art date
Application number
BRPI0509846-7A
Other languages
English (en)
Inventor
Andreas Herrmann
Ingeborg Dreher
Thomas Moll
Stefanie Zahn
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BRPI0509846A publication Critical patent/BRPI0509846A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5443—IL-15
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SISTEMA DE EXPRESSãO PARA A PREPARAçãO DE PROTEìNAS DE FUSãO DE IL-l5/Fc E SEU USO. A invenção refere-se a um sistema de expressão contendo um ou mais ácidos nucléicos compreendendo pelo menos um ácido nucléico para uma proteína de fusão de interleucina 15/Fc (IL-15/Fc), pelo menos um promotor, pelo menos um ácido nucléico para um CD5 Líder, onde apropriado, pelo menos um ácido nucléico para um marcador de gene selecionável; a um ácido nucléico compreendendo os componentes do dito sistema de expressão e a uma célula hospedeira contendo o sistema de expressão ou o ácido nucléico. A invenção refere-se ainda a um processo para a preparação de uma proteína de fusão de IL-15/Fc, usando-se o sistema de expressão, e ao uso do sistema de expressão, o ácido nucléico, a célula hospedeira ou o CD5 Líder para a expressão nas células hospedeiras.
BRPI0509846-7A 2004-04-14 2005-04-13 sistema de expressão para a preparação de proteìnas de fusão de il-15/fc e seu uso BRPI0509846A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04008881A EP1586585A1 (de) 2004-04-14 2004-04-14 Expressionssystem zur Herstellung von IL-15/Fc-Fusionsproteinen und ihre Verwendung
PCT/EP2005/003888 WO2005100395A2 (en) 2004-04-14 2005-04-13 EXPRESSION SYSTEM FOR PREPARING IL-15/Fc FUSION PROTEINS AND ITS USE

Publications (1)

Publication Number Publication Date
BRPI0509846A true BRPI0509846A (pt) 2007-10-09

Family

ID=34924596

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0509846-7A BRPI0509846A (pt) 2004-04-14 2005-04-13 sistema de expressão para a preparação de proteìnas de fusão de il-15/fc e seu uso

Country Status (11)

Country Link
US (1) US20090117618A1 (pt)
EP (2) EP1586585A1 (pt)
JP (1) JP2007535919A (pt)
KR (1) KR20070002052A (pt)
CN (1) CN1942481A (pt)
AU (1) AU2005233283A1 (pt)
BR (1) BRPI0509846A (pt)
CA (1) CA2562761A1 (pt)
MX (1) MXPA06009495A (pt)
RU (1) RU2006140084A (pt)
WO (1) WO2005100395A2 (pt)

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Publication number Priority date Publication date Assignee Title
US7625564B2 (en) 2006-01-27 2009-12-01 Novagen Holding Corporation Recombinant human EPO-Fc fusion proteins with prolonged half-life and enhanced erythropoietic activity in vivo
KR100888022B1 (ko) * 2006-12-21 2009-03-09 재단법인 목암생명공학연구소 면역글로불린 Ｆｃ와 인간 아포리포단백질（ａ）크링글절편의 융합단백질 ＬＫ8­Ｆｃ
ES2642008T3 (es) 2007-05-11 2017-11-14 Altor Bioscience Corporation Moléculas de fusión y variantes de IL-15
BRPI0814465B1 (pt) 2007-07-26 2021-11-23 Novagen Holding Corporation Proteína de fusão, dímero, método para produzir uma proteína de fusão, linhagem de célula, usos de uma proteína de fusão e de uma composição farmacêutica e composição farmacêutica
SG177764A1 (en) 2009-07-30 2012-03-29 Hoffmann La Roche Enzymatic antibody processing
EP3851459B1 (en) 2010-09-21 2024-05-01 Altor BioScience, LLC Multimeric il-15 soluble fusion molecules and methods of making and using same
US11053299B2 (en) 2010-09-21 2021-07-06 Immunity Bio, Inc. Superkine
WO2012068134A1 (en) * 2010-11-15 2012-05-24 Biogen Idec Inc. Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography
WO2013004587A1 (en) 2011-07-01 2013-01-10 F. Hoffmann-La Roche Ag Method for separation of monomeric polypeptides from aggregated polypeptides
CN102690354B (zh) * 2012-05-14 2015-03-25 安源生物科技(上海)有限公司 重组二聚化抗凝血酶III-Fc融合蛋白及其哺乳动物细胞高效表达系统
US9816041B2 (en) 2013-12-09 2017-11-14 Swift Fuels, Llc Aviation gasolines containing mesitylene and isopentane
RU2689717C2 (ru) 2014-01-08 2019-05-28 Шанхай Хэнжуй Фармасьютикал Ко., Лтд. Гетеродимерный белок il-15 и его применения
US10464982B2 (en) 2014-04-23 2019-11-05 Emory University Compositions of GM-CSF and interleukin fusions for immune modulation and uses related thereto
KR102762243B1 (ko) 2014-06-30 2025-02-05 알토 바이오사이언스 엘엘씨 Il-15-베이즈드 분자 및 이의 사용 방법
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
EP3307783B1 (en) 2015-06-10 2021-01-06 Emory University Compositions and conjugates comprising an interleukin and polypeptides that specifically bind tgf-beta
KR102463844B1 (ko) * 2016-05-27 2022-11-08 알토 바이오사이언스 코포레이션 Cd3 결합 도메인을 가지는 다량체 il-15 기반 분자의 구성 및 특성규명
EP3529263B1 (en) 2016-10-21 2025-08-06 Altor BioScience Corporation Multimeric il-15-based molecules
TWI791894B (zh) 2018-09-21 2023-02-11 大陸商信達生物製藥（蘇州）有限公司 新型白介素２及其用途
AU2019344875B2 (en) 2018-09-21 2021-12-23 Innovent Biologics (Suzhou) Co., Ltd. Novel interleukin 2 and use thereof
EP3870331B1 (en) * 2018-10-25 2025-07-23 F. Hoffmann-La Roche AG Modification of antibody fcrn binding
EP4028413A1 (en) 2019-09-10 2022-07-20 Obsidian Therapeutics, Inc. Ca2-il15 fusion proteins for tunable regulation
CN111690069B (zh) * 2019-11-13 2022-04-19 中国科学技术大学 一种IL-15/SuIL-15Rα-mFc-γ4复合体蛋白及其构造方法、应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5225538A (en) * 1989-02-23 1993-07-06 Genentech, Inc. Lymphocyte homing receptor/immunoglobulin fusion proteins
US5977307A (en) * 1989-09-07 1999-11-02 Alkermes, Inc. Transferrin receptor specific ligand-neuropharmaceutical agent fusion proteins
US5851795A (en) * 1991-06-27 1998-12-22 Bristol-Myers Squibb Company Soluble CTLA4 molecules and uses thereof
US7279568B2 (en) * 1998-12-31 2007-10-09 Viromed Limited Highly efficient eukaryotic expression vector comprising an exogenous transcription regulatory element
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CA2428242A1 (en) * 2000-11-07 2002-05-16 Zymogenetics, Inc. Human tumor necrosis factor receptor
AU2003230834A1 (en) * 2002-04-09 2003-10-27 Beth Israel Deaconess Medical Center, Inc. Antagonists of il-21 and modulation of il-21-mediated t cell responses
AU2003269659A1 (en) * 2002-10-14 2004-05-04 F. Hoffmann-La Roche Ag Antagonists il-15
US20070072271A1 (en) * 2004-01-07 2007-03-29 Novo Nordisk Healthcare A/G Method for the production of recombinant proteins
TW200619227A (en) * 2004-08-11 2006-06-16 Hoffmann La Roche Mutant interleukin-15-containing compositions and suppression of an immune response

Also Published As

Publication number Publication date
EP1586585A1 (de) 2005-10-19
MXPA06009495A (es) 2007-03-30
RU2006140084A (ru) 2008-05-20
WO2005100395A3 (en) 2006-06-01
CN1942481A (zh) 2007-04-04
US20090117618A1 (en) 2009-05-07
CA2562761A1 (en) 2005-10-27
JP2007535919A (ja) 2007-12-13
KR20070002052A (ko) 2007-01-04
WO2005100395A2 (en) 2005-10-27
AU2005233283A1 (en) 2005-10-27
EP1756154A2 (en) 2007-02-28

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]