BRPI0511046A - methods for producing a multiple expression construct, for producing food or food, pharmaceuticals or chemicals, and a transgenic plant or plant cell, multiple expression construct, microorganism, and, use of a microorganism, a plant cell , a plant tissue, a plant organ, or a part of a plant - Google Patents

methods for producing a multiple expression construct, for producing food or food, pharmaceuticals or chemicals, and a transgenic plant or plant cell, multiple expression construct, microorganism, and, use of a microorganism, a plant cell , a plant tissue, a plant organ, or a part of a plant

Info

Publication number
BRPI0511046A
BRPI0511046A BRPI0511046-7A BRPI0511046A BRPI0511046A BR PI0511046 A BRPI0511046 A BR PI0511046A BR PI0511046 A BRPI0511046 A BR PI0511046A BR PI0511046 A BRPI0511046 A BR PI0511046A
Authority
BR
Brazil
Prior art keywords
plant
producing
food
microorganism
expression construct
Prior art date
Application number
BRPI0511046-7A
Other languages
Portuguese (pt)
Inventor
Linda Patricia Loyall
Original Assignee
Basf Plant Science Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh filed Critical Basf Plant Science Gmbh
Publication of BRPI0511046A publication Critical patent/BRPI0511046A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MéTODOS PARA PRODUZIR UMA CONSTRUçãO DE EXPRESSãO MúLTIPLA, PARA PRODUZIR ALIMENTO OU PRODUTOS ALIMENTìCIOS, PRODUTOS FARMACêUTICOS OU PRODUTOS QUìMICOS, E UMA PLANTA OU CéLULA DE PLANTA TRANSGêNICA, CONSTRUçãO DE EXPRESSãO MúLTIPLA, MICROORGANISMO, E, USO DE UM MICROORGANISMO, DE UMA CéLULA DE PLANTA, DE UM TECIDO DE PLANTA, DE UM óRGãO DE PLANTA, OU DE UMA PARTE DE UMA PLANTA. A presente invenção diz respeito a métodos para a montagem de construções com cassetes de expressão de gene múltiplos pela clonagem recombinacional direcionada. O DNA e vetores tendo sítios de recombinação engendrados são fornecidos para o uso em um método de clonagem recombinacional que possibilita a montagem eficiente e específica de cassetes de expressão a uma Construção de Expressão Múltipla em uma etapa de clonagem recombinacional única.METHODS FOR PRODUCING A MULTIPLE EXPRESSION CONSTRUCTION, FOR PRODUCING FOOD OR FOOD PRODUCTS, PHARMACEUTICAL PRODUCTS OR A TRANSGENIC PLANT PLANT, CELL, MICROPRODUCTION EXGRANS, MICROPRODUCTION PLANT, CELL , A PLANT TISSUE, PLANT ORGAN, OR PART OF A PLANT. The present invention relates to methods for assembling multiple gene expression cassette constructs by directed recombinational cloning. DNA and vectors having engineered recombination sites are provided for use in a recombinational cloning method that enables efficient and specific assembly of expression cassettes to a Multiple Expression Construct in a single recombinational cloning step.

BRPI0511046-7A 2004-05-10 2005-04-28 methods for producing a multiple expression construct, for producing food or food, pharmaceuticals or chemicals, and a transgenic plant or plant cell, multiple expression construct, microorganism, and, use of a microorganism, a plant cell , a plant tissue, a plant organ, or a part of a plant BRPI0511046A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04011041 2004-05-10
PCT/EP2005/004545 WO2005108568A1 (en) 2004-05-10 2005-04-28 Methods for assembling multiple expression constructs

Publications (1)

Publication Number Publication Date
BRPI0511046A true BRPI0511046A (en) 2007-11-27

Family

ID=34968243

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0511046-7A BRPI0511046A (en) 2004-05-10 2005-04-28 methods for producing a multiple expression construct, for producing food or food, pharmaceuticals or chemicals, and a transgenic plant or plant cell, multiple expression construct, microorganism, and, use of a microorganism, a plant cell , a plant tissue, a plant organ, or a part of a plant

Country Status (6)

Country Link
EP (1) EP1629096A1 (en)
CN (1) CN1981039A (en)
AU (1) AU2005240741A1 (en)
BR (1) BRPI0511046A (en)
CA (1) CA2564039A1 (en)
WO (1) WO2005108568A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006212647A1 (en) 2005-02-09 2006-08-17 Bioriginal Food & Science Corp. Novel omega-3 fatty acid desaturase family members and uses thereof
CA2698627A1 (en) * 2007-10-05 2009-04-09 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts
AU2013203045B2 (en) * 2007-10-05 2014-06-19 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts
US8314222B2 (en) 2007-10-05 2012-11-20 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts
CA3185756A1 (en) 2012-12-24 2014-07-03 Coagulant Therapeutics Corporation Short-acting factor vii polypeptides
PE20170261A1 (en) 2014-05-13 2017-04-12 Univ Pennsylvania COMPOSITIONS THAT INCLUDE ADENO-ASSOCIATED VIRUS (AAV) THAT EXPRESSES DUAL ANTIBODY CONSTRUCTS AND THEIR USES
CN107429246B (en) * 2014-10-31 2021-06-01 麻省理工学院 Massively parallel combinatorial genetics for CRISPR
CN110257444B (en) * 2019-03-08 2023-04-25 海南大学 A method of producing medium-chain fatty acids in plant cells
JP7644722B2 (en) * 2019-06-01 2025-03-12 シベック バイオテクノロジーズ，インコーポレイティド A bacterial platform for the delivery of gene editing systems into eukaryotic cells
CN110408645B (en) * 2019-08-14 2021-01-08 华中科技大学 Multiple traceless integration system and method for target gene in yarrowia lipolytica
CN110904143A (en) * 2019-09-12 2020-03-24 黑龙江省农业科学院耕作栽培研究所 Multifunctional glyphosate-resistant rice transformation vector pCDMAR-epsps and construction method and application thereof
IL295387A (en) 2020-02-05 2022-10-01 Larimar Therapeutics Inc Peptide-binding proteins and their uses
CN114457098B (en) * 2022-01-07 2022-12-27 广东省农业科学院蔬菜研究所 Multi-gene vector system and application thereof
CN114561457B (en) * 2022-01-10 2026-02-03 深圳市仙湖植物园(深圳市园林研究中心) Molecular marker, primer pair, application of primer pair and identification method of sex of cycadae
CN115896169A (en) * 2023-02-07 2023-04-04 西南大学 Multifunctional plant binary expression vector pRC10 and its construction method and application
CN118166023A (en) * 2024-02-26 2024-06-11 广东省农业科学院果树研究所 Simple, convenient and efficient citrus genetic transformation final vector and construction method and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
EP1373527A1 (en) * 2001-04-06 2004-01-02 CropDesign N.V. The use of double and opposite recombination sites for the single step cloning of two dna segments
JP2004531259A (en) * 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション Compositions and methods for recombinant cloning of nucleic acid molecules
AU2003273995A1 (en) * 2002-06-05 2003-12-22 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
CN1263860C (en) * 2002-09-30 2006-07-12 华南农业大学 Construction method of multigene carrier and its application

Also Published As

Publication number Publication date
AU2005240741A1 (en) 2005-11-17
CA2564039A1 (en) 2005-11-17
WO2005108568A1 (en) 2005-11-17
CN1981039A (en) 2007-06-13
EP1629096A1 (en) 2006-03-01

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]