BRPI0515418A - método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria - Google Patents

método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria

Info

Publication number
BRPI0515418A
BRPI0515418A BRPI0515418-9A BRPI0515418A BRPI0515418A BR PI0515418 A BRPI0515418 A BR PI0515418A BR PI0515418 A BRPI0515418 A BR PI0515418A BR PI0515418 A BRPI0515418 A BR PI0515418A
Authority
BR
Brazil
Prior art keywords
matter
composition
production
plasmid dna
covalently closed
Prior art date
Application number
BRPI0515418-9A
Other languages
English (en)
Inventor
Clague P Hodgson
James A Williams
Original Assignee
Nature Technology Corp
Clague P Hodgson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nature Technology Corp, Clague P Hodgson filed Critical Nature Technology Corp
Publication of BRPI0515418A publication Critical patent/BRPI0515418A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MéTODO PARA A PRODUçãO DE DNA DE PLASMìDIO SUPER-ENROLADO FECHADO COVALENTEMENTE E COMPOSIçãO DE MATéRIA. Um método geral e cepas de bactérias são descritos, por meio do qual é possível purificar drasticamente o DNA de plasmídio com respeito ao DNA genómico, e o RNA. Em uma realização preterida, a lise e a remoçãoda nuclease de ácidos nucléicos hospedeiros é um componente integral do processo de fermentação/colheita, e dessa maneira é obtido um rendimento maior e uma pureza com a purificação a jusante simplificada e correntes residuais reduzidas, reduzindo desse modo os custos de produção.
BRPI0515418-9A 2004-08-16 2005-08-15 método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria BRPI0515418A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60207404P 2004-08-16 2004-08-16
PCT/US2005/028870 WO2006026125A2 (en) 2004-08-16 2005-08-15 Improved strains of e. coli for plasmid dna production

Publications (1)

Publication Number Publication Date
BRPI0515418A true BRPI0515418A (pt) 2008-07-22

Family

ID=36000523

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0515418-9A BRPI0515418A (pt) 2004-08-16 2005-08-15 método para a produção de dna de plasmìdio super-enrolado fechado covalentemente e composição de matéria

Country Status (14)

Country Link
US (1) US8748168B2 (pt)
EP (1) EP1781801A4 (pt)
JP (1) JP2008509704A (pt)
KR (1) KR20070053268A (pt)
CN (1) CN101035905A (pt)
AU (1) AU2005280356B2 (pt)
BR (1) BRPI0515418A (pt)
CA (1) CA2577419A1 (pt)
EA (1) EA010864B1 (pt)
IL (1) IL181319A0 (pt)
MX (1) MX2007001847A (pt)
NZ (1) NZ553658A (pt)
WO (1) WO2006026125A2 (pt)
ZA (1) ZA200701265B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2431470B1 (en) * 2006-11-30 2017-11-29 Novozymes A/S Dnase expression in recombinant host cells
EP2160459A2 (en) 2007-05-23 2010-03-10 Nature Technology Corp. Improved e. coli plasmid dna production
CA2721153C (en) * 2008-04-10 2018-10-02 Abraxis Bioscience, Llc Compositions of hydrophobic taxane derivatives and uses thereof
CA3031231A1 (en) * 2016-08-08 2018-02-15 Karius, Inc. Reduction of signal from contaminant nucleic acids
CN108220317B (zh) * 2017-12-11 2021-04-16 北京大学 一种重组表达质粒及其制备方法、用途
GB201814590D0 (en) * 2018-09-07 2018-10-24 Oxford Biomedica Ltd Viral vector production system
CN116179581B (zh) * 2022-07-15 2025-11-28 北京化工大学 一种用于大肠杆菌自裂解的裂解模块、系统及其应用
CN116904560A (zh) * 2023-08-22 2023-10-20 通用生物(安徽)股份有限公司 一种质粒当中宿主dna残留测定方法
CN120624490B (zh) * 2025-05-28 2026-05-26 浙江毓昌生物技术有限公司 一种基于双启动子表达质粒的包涵体的制备方法及其应用

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE50792T1 (de) 1983-04-21 1990-03-15 Australia Gentech Ltd Herstellung von plasmid-dns und produkte davon.
WO1992013963A1 (en) 1991-01-30 1992-08-20 Hyman Edward D Method for preparation of closed circular dna
FR2728264B1 (fr) 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
US6455287B1 (en) 1995-02-23 2002-09-24 Wyeth Mechanical disruption of bacterial cells for plasmid recovery
US5591064A (en) 1995-06-30 1997-01-07 Church & Dwight Co., Inc. Blasting apparatus and method for blast cleaning a solid surface
FR2746412B1 (fr) 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
MXPA00009561A (es) 1998-03-30 2002-08-06 Metabolix Inc Cepas microbianas y procesos para la fabricacion de biomateriales.
EP0987327A1 (en) * 1998-09-14 2000-03-22 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
EP0992583B1 (en) * 1998-09-14 2003-03-26 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
PT1124941E (pt) * 1998-10-28 2004-02-27 Genentech Inc Processo para recuperar polipeptidos heterologos a partir de celulas bacterianas
AU1031900A (en) 1998-11-09 2000-05-29 Genecare Development Aps Novel plasmids for use in medicine and method of producing same
GB9924973D0 (en) 1999-10-21 1999-12-22 Cobra Therapeutics Ltd Preparation of cellular components that are substantially free of chromosomal DNA
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
CA2414775A1 (en) 2000-05-03 2001-11-08 Expressive Constructs, Inc. A method and device for improving protein stability and solubility
US20020197637A1 (en) 2001-06-02 2002-12-26 Willson Richard C. Process and compositions for protection of nucleic acids
US20030092885A1 (en) 2001-07-31 2003-05-15 President And Fellows Of Harvard College Solubility and stability enhancement tag for structural and ligand binding studies of proteins
AU2002321931A1 (en) 2001-11-20 2003-06-10 Atgen Co., Ltd. Novel peptides conferring environmental stress resistance and fusion proteins including said peptides
GB0127809D0 (en) 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid

Also Published As

Publication number Publication date
WO2006026125A2 (en) 2006-03-09
CN101035905A (zh) 2007-09-12
NZ553658A (en) 2009-11-27
US20070249042A1 (en) 2007-10-25
US8748168B2 (en) 2014-06-10
ZA200701265B (en) 2008-04-30
JP2008509704A (ja) 2008-04-03
WO2006026125A3 (en) 2006-07-06
EA010864B1 (ru) 2008-12-30
AU2005280356A1 (en) 2006-03-09
EA200700415A1 (ru) 2007-10-26
EP1781801A2 (en) 2007-05-09
IL181319A0 (en) 2007-07-04
AU2005280356B2 (en) 2008-09-25
EP1781801A4 (en) 2008-11-12
CA2577419A1 (en) 2006-03-09
MX2007001847A (es) 2007-04-13
KR20070053268A (ko) 2007-05-23

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2160 DE 29/05/2012.