BRPI0515922B8 - Molécula de dna, kit, constructo, método de identificação do evento, método de detecção, par de moléculas de dna, método de confirmação da pureza, método de varredura, par de sequências de dna - Google Patents

Molécula de dna, kit, constructo, método de identificação do evento, método de detecção, par de moléculas de dna, método de confirmação da pureza, método de varredura, par de sequências de dna

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Publication number
BRPI0515922B8
BRPI0515922B8 BRPI0515922A BRPI0515922A BRPI0515922B8 BR PI0515922 B8 BRPI0515922 B8 BR PI0515922B8 BR PI0515922 A BRPI0515922 A BR PI0515922A BR PI0515922 A BRPI0515922 A BR PI0515922A BR PI0515922 B8 BRPI0515922 B8 BR PI0515922B8
Authority
BR
Brazil
Prior art keywords
dna
pair
das
dna molecule
event
Prior art date
Application number
BRPI0515922A
Other languages
English (en)
Inventor
Wayne Bing James
F Cressman Robert Jr
Gupta Manju
M Hakimi Salim
Hondred David
L Krone Todd
E Hartnett Locke Mary
K Luckring Abigail
E Meyer Sandra
Moellenbeck Daniel
Edwin Narva Kenneth
D Olson Paul
D Sanders Craig
Wang Jimei
Zhang Jian
Zhong Gan-Yuan
Original Assignee
Dow Agrosciences Llc
Pioneer Hi Bred Int
Du Pont
Corteva Agriscience Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Pioneer Hi Bred Int, Du Pont, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BRPI0515922A publication Critical patent/BRPI0515922A/pt
Publication of BRPI0515922B1 publication Critical patent/BRPI0515922B1/pt
Publication of BRPI0515922B8 publication Critical patent/BRPI0515922B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MOLÉCULAS DE DNA ISOLADAS, PAR DE MOLÉCULAS DE DNA, SEQUÊNCIA DE DNA ISOLADA, PAR DE SEQUÊNCIAS DE DNA ISOLADAS, SEQUÊNCIA DE INICIADOR DE NUCLEOTÍDEO DE DNA ISOLADA, CONSTRUCTO DE DNA, KIT E MÉTODO DE IDENTIFICAÇÃO DO EVENTO DAS - 59122-7 EM AMOSTRA BIOLÓGICA, KIT DE DETECÇÃO DE DNA, MÉTODOS DE DETECÇÃO DA PRESENÇA DO EVENTO DAS-59122-7 E DE DNA CORRESPONDENTE AO EVENTO DAS-59122-7, MÉTODOS DE VARREDURA E DE CONFIRMAÇÃO DA PUREZA DE SEMENTE, MÉTODO DE PRODUÇÃO DE PLANTA DE MILHO RESISTENTE A INSETOS, MÉTODO DE DETECÇÃO DA PRESENÇA DE INSERÇÃO DO EVENTO DAS-59122-7 EM TECIDO DE MILHO A presente invenção refere-se a composições de DNA relacionadas a plantas transgênicas de milho resistentes a insetos. A presente invenção refere-se ainda a ensaios de detecção da presença do envio DAS-59122-7 de milho, baseados na sequência de DNA do constructo recombinante inserido no genoma do milho e nas sequências de DNA que flanqueiam o sítio de inserção. Também são fornecidos kits e condições úteis na realização dos ensaios da presente invenção.
BRPI0515922A 2004-09-29 2005-09-28 Molécula de dna, kit, constructo, método de identificação do evento, método de detecção, par de moléculas de dna, método de confirmação da pureza, método de varredura, par de sequências de dna BRPI0515922B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61422504P 2004-09-29 2004-09-29
PCT/US2005/034947 WO2006039376A2 (en) 2004-09-29 2005-09-28 Corn event das-59122-7 and methods for detection thereof

Publications (3)

Publication Number Publication Date
BRPI0515922A BRPI0515922A (pt) 2008-08-12
BRPI0515922B1 BRPI0515922B1 (pt) 2018-10-09
BRPI0515922B8 true BRPI0515922B8 (pt) 2022-12-06

Family

ID=36143043

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0515922A BRPI0515922B8 (pt) 2004-09-29 2005-09-28 Molécula de dna, kit, constructo, método de identificação do evento, método de detecção, par de moléculas de dna, método de confirmação da pureza, método de varredura, par de sequências de dna

Country Status (15)

Country Link
US (17) US7323556B2 (pt)
EP (1) EP1794308B1 (pt)
JP (2) JP2008514233A (pt)
KR (1) KR101337016B1 (pt)
AR (1) AR050891A1 (pt)
AU (1) AU2005292090B2 (pt)
BR (1) BRPI0515922B8 (pt)
CA (1) CA2588243C (pt)
CL (1) CL2018002146A1 (pt)
ES (1) ES2432749T3 (pt)
NZ (1) NZ554035A (pt)
PL (1) PL1794308T3 (pt)
TW (1) TWI414236B (pt)
UA (1) UA97088C2 (pt)
WO (1) WO2006039376A2 (pt)

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