BRPI0519923A2 - polypeptide, dna sequence, dna vector, antibody, bacterium, method for producing partially 3-o-deacylated lps, composition, uses of the bacterium, and isolated bordetella lps comprising at least partially 3-o-deacylated lps, vaccine, and, in vitro method for deacylating gram-negative lps or compositions comprising gram-negative lps. - Google Patents

polypeptide, dna sequence, dna vector, antibody, bacterium, method for producing partially 3-o-deacylated lps, composition, uses of the bacterium, and isolated bordetella lps comprising at least partially 3-o-deacylated lps, vaccine, and, in vitro method for deacylating gram-negative lps or compositions comprising gram-negative lps.

Info

Publication number
BRPI0519923A2
BRPI0519923A2 BRPI0519923-9A BRPI0519923A BRPI0519923A2 BR PI0519923 A2 BRPI0519923 A2 BR PI0519923A2 BR PI0519923 A BRPI0519923 A BR PI0519923A BR PI0519923 A2 BRPI0519923 A2 BR PI0519923A2
Authority
BR
Brazil
Prior art keywords
lps
gram
negative
bacterium
deacylated
Prior art date
Application number
BRPI0519923-9A
Other languages
Portuguese (pt)
Inventor
Johannes Petrus Maria Tommassen
Peter Andre Van Der Ley
Jeroen Johannes Gerardus Geurtsen
Original Assignee
Nvi Nl Vaccininst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nvi Nl Vaccininst filed Critical Nvi Nl Vaccininst
Publication of BRPI0519923A2 publication Critical patent/BRPI0519923A2/en
Publication of BRPI0519923A8 publication Critical patent/BRPI0519923A8/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/02Bacterial antigens
    • A61K39/099Bordetella
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/235Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

POLIPEPTìDEO, SEQüêNCIA DE DNA, VETOR DE DNA, ANTICORPO, BACTéRIA, MéTODO PARA PRODUZIR LPS PARCIALMENTE 3-O-DESACILADO, COMPOSIçãO, USOS DA BACTERIA, E DE LPS DE BORDETELLA ISOLADO COMPREENDENDO LPS PELO MENOS PARCIALMENTE 3-O-DESACILADO, VACINA, E, MéTODO IN VITRO PARA A DESACILAçãO DE LPS GRAM-NEGATIVO OU DE COMPOSIçõES COMPREENDENDO LPS GRAM-NEGATIVO. A presente invenção proporciona novos polipeptídeos Gram- negativos exibindo atividade de lipídeo A 3-O-desacilase e que são capazes de modificar e/ou desintoxicar LPS Gram-negativos. A presente invenção também proporciona bactérias Gram-negativas, lipopolissacarídeos (LPS) de bactérias Gram-negativas e composições compreendendo LPS, que são proporcionadas com ou tratadas com uma atividade de 3-O-desacilase de acordo com a invenção e que podem ser utilizadas para propósitos farmacêuticos e/ou veterinários, em particular para a preparação de vacinas acelulares ou de célula inteira contra patógenos Gram-negativos tais como Bordeteila pertuss is, Bordeteila parapertuss is, e Bordetelia bronchiseptica.Polypeptide, DNA Sequence, DNA VECTOR, ANTIBODY, BACTERIA, METHOD FOR PRODUCING PARTIALLY 3-O-DISABLED LPS, COMPOSITION, USES OF BACTERIA, AND LPS OF BORDETELLA ISOLATED COMPLETE LACIN-LPS PLATE AND, IN VITRO METHOD FOR DISABILING GRAM NEGATIVE LPS OR COMPOSITIONS UNDERSTANDING GRAM NEGATIVE LPS. The present invention provides novel Gram-negative polypeptides exhibiting 3-O-deacylase lipid A activity and which are capable of modifying and / or detoxifying Gram-negative LPS. The present invention also provides Gram-negative bacteria, Gram-negative lipopolysaccharide (LPS) bacteria, and compositions comprising LPS, which are provided with or treated with a 3-O-deacylase activity according to the invention and which may be used for pharmaceutical and / or veterinary purposes, in particular for the preparation of acellular or whole cell vaccines against Gram-negative pathogens such as Bordeteila pertussis, Bordeteila parapertussis, and Bordetelia bronchiseptica.

BRPI0519923A 2004-12-17 2005-12-16 polypeptide, dna sequence, dna vector, antibody, bacterium, method for producing partially 3-0-deacylated lps, composition, uses of the bacterium, and isolated bordetella lps comprising at least partially 3-0-deacylated lps, vaccine, and, in vitro method for deacylating gram-negative lps or compositions comprising gram-negative lps, and for inducing an immune response against bordetella. BRPI0519923A8 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04078445 2004-12-17
PCT/NL2005/050081 WO2006065139A2 (en) 2004-12-17 2005-12-16 Deacylation of lps in gram negative bacteria

Publications (2)

Publication Number Publication Date
BRPI0519923A2 true BRPI0519923A2 (en) 2009-08-11
BRPI0519923A8 BRPI0519923A8 (en) 2018-01-23

Family

ID=34928754

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0519923A BRPI0519923A8 (en) 2004-12-17 2005-12-16 polypeptide, dna sequence, dna vector, antibody, bacterium, method for producing partially 3-0-deacylated lps, composition, uses of the bacterium, and isolated bordetella lps comprising at least partially 3-0-deacylated lps, vaccine, and, in vitro method for deacylating gram-negative lps or compositions comprising gram-negative lps, and for inducing an immune response against bordetella.

Country Status (15)

Country Link
US (1) US8048433B2 (en)
EP (1) EP1828378B1 (en)
JP (1) JP5378685B2 (en)
CN (1) CN101203605B (en)
AU (1) AU2005317304B2 (en)
BR (1) BRPI0519923A8 (en)
CA (1) CA2590906C (en)
DK (1) DK1828378T3 (en)
ES (1) ES2493440T3 (en)
MX (1) MX2007007288A (en)
NO (1) NO340331B1 (en)
NZ (1) NZ555889A (en)
PL (1) PL1828378T3 (en)
PT (1) PT1828378E (en)
WO (1) WO2006065139A2 (en)

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ES2393682T3 (en) * 2007-03-26 2012-12-27 De Staat Der Nederlanden, Vert. Door De Minister Van Vws Enhanced vaccines against Bordotella pertussis based on LPS glycosyltransferase mutants
WO2014024026A1 (en) 2012-08-06 2014-02-13 Glaxosmithkline Biologicals S.A. Method for eliciting in infants an immune response against rsv and b. pertussis
US20140037680A1 (en) 2012-08-06 2014-02-06 Glaxosmithkline Biologicals, S.A. Novel method
EP3006450B1 (en) 2013-06-04 2020-07-29 Aparin, Petr Gennadievich Modified endotoxic bacteria lipopolysaccharide (variants), combination of modified lipopolysaccharides (variants) and, containing same, a vaccine (variants) and a pharmaceutical composition (variants)
US11052142B2 (en) 2013-06-04 2021-07-06 Petr G. Aparin Modified endotoxic bacteria lipopolysaccharide (variants), combination of modified lipopolysaccharides (variants) and, containing same, a vaccine (variants) and a pharmaceutical composition (variants)
AU2014304545A1 (en) 2013-08-05 2016-02-25 Glaxosmithkline Biologicals S.A. Combination immunogenic compositions
WO2015179270A2 (en) 2014-05-19 2015-11-26 Board Of Regents, The University Of Texas System Combinatorial platform for the display of surface adjuvants and antigens
CN104388368B (en) * 2014-10-30 2017-02-15 四川农业大学 Low-endotoxin escherichia coli prokaryotic expression engineering bacterial mutant strain and construction method
CA3011282A1 (en) * 2016-01-28 2017-08-03 De Staat Der Nederlanden, Vert. Door De Minister Van Vws, Ministerie Van Volksgezondheid, Welzijn En Sport Modified tetra-acylated neisserial lps
CN105567620B (en) * 2016-01-29 2019-03-19 江南大学 A Cronobacter sakazakii mutant strain producing attenuated lipid A and its application
CN105695540A (en) * 2016-03-18 2016-06-22 辽宁成大生物股份有限公司 Preparing method of gram-negative bacterium cell wall antigen
BR112019018905A2 (en) 2017-03-13 2020-05-19 De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport vaccines against bordetella comprising lps with reduced reactogenicity
WO2018213242A1 (en) * 2017-05-15 2018-11-22 University Of Maryland, Baltimore Live salmonella typhi vectors engineered to express heterologous outer membrane protein antigens and methods of use thereof
BR112020011019A2 (en) * 2017-12-04 2020-11-17 De Staat Der Nederlanden, Vert. Door De Minister Van Vws, Ministerie Van Volksgezondheid, Welzijn En Sport improved process to produce outer membrane vesicles
CN115552020B (en) * 2020-05-14 2026-01-09 默克专利股份公司 Methods and compositions for purifying adeno-associated virus particles or adenovirus
US20250263447A1 (en) 2020-09-04 2025-08-21 Intravacc B.V. Ompa mutations enhance omv production in bordetella pertussis
EP4169513A1 (en) 2021-10-19 2023-04-26 GlaxoSmithKline Biologicals S.A. Adjuvant composition comprising sting agonists

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CH650681A5 (en) * 1981-08-14 1985-08-15 Dso Pharmachim Whooping cough vaccine
KR0168039B1 (en) * 1987-09-04 1999-01-15 로버트 디. 웨스트 Recombinant dna derived bordetella toxin subunit analogs
EP0484621A3 (en) * 1990-07-11 1992-08-26 American Cyanamid Company Efficacious vaccines against bordetella pertussis comprising a combination of individually purified pertussis antigens
CZ285307B6 (en) * 1992-08-18 1999-07-14 Dimminaco Ag/Sa/Ltd. Polypeptide exhibiting immunogenic properties of bordetella brochiseptica fimbria protein, recombinant polynucleotide capable of inducing expression of such polypeptide and vaccine against infections caused by bordetella brochiseptica bacteria
ATE221081T1 (en) * 1995-05-04 2002-08-15 Aventis Pasteur ACELLULAR PERTUSSIS VACCINES AND METHOD FOR THE PRODUCTION THEREOF
US6887483B2 (en) 1995-12-01 2005-05-03 University Of Iowa Research Foundation Non-toxic mutants of pathogenic gram-negative bacteria
NZ338101A (en) * 1997-04-01 2002-03-28 Corixa Corp Adjuvant compositions of Monophosphoryl Lipid A (MPL) and a phospholipid based surfactant (1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)) suitable for intranasal administration
CA2264970A1 (en) 1998-03-10 1999-09-10 American Cyanamid Company Antigenic conjugates of conserved lipolysaccharides of gram negative bacteria
AU762369B2 (en) * 1998-11-03 2003-06-26 De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezonheid En Cultuur LPS with reduced toxicity from genetically modified gram negative bacteria
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AU2002307448A1 (en) * 2001-04-20 2002-11-05 President And Fellows Of Harvard College Methods and compositions for the modulation of biofilm formation

Also Published As

Publication number Publication date
AU2005317304B2 (en) 2011-06-23
BRPI0519923A8 (en) 2018-01-23
US8048433B2 (en) 2011-11-01
ES2493440T3 (en) 2014-09-11
NO20073088L (en) 2007-09-17
NO340331B1 (en) 2017-04-03
PT1828378E (en) 2014-10-10
EP1828378B1 (en) 2014-06-11
WO2006065139A3 (en) 2006-10-19
CN101203605A (en) 2008-06-18
MX2007007288A (en) 2008-02-25
DK1828378T3 (en) 2014-09-01
JP2008523805A (en) 2008-07-10
JP5378685B2 (en) 2013-12-25
WO2006065139A2 (en) 2006-06-22
CA2590906A1 (en) 2006-06-22
NZ555889A (en) 2009-07-31
CA2590906C (en) 2017-01-03
US20080274145A1 (en) 2008-11-06
PL1828378T3 (en) 2014-10-31
CN101203605B (en) 2016-01-27
EP1828378A2 (en) 2007-09-05
AU2005317304A1 (en) 2006-06-22

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B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]

Free format text: SOLICITA-SE A REGULARIZACAO DA PROCURACAO, UMA VEZ QUE BASEADO NO ARTIGO 216 1O DA LPI, O DOCUMENTO DE PROCURACAO DEVE SER APRESENTADO EM SUA FORMA AUTENTICADA; OU SEGUNDO PARECER DA PROCURADORIA NO 074/93, DEVE CONSTAR UMA DECLARACAO DE VERACIDADE, A QUAL DEVE SER ASSINADA POR UMA PESSOA DEVIDAMENTE AUTORIZADA A REPRESENTAR O INTERESSADO, DEVENDO A MESMA CONSTAR NO INSTRUMENTO DE PROCURACAO, OU NO SEU SUBSTABELECIMENTO.

B25A Requested transfer of rights approved

Owner name: DE STAAT DER NEDERLANDEN, VERTEGENWOORDIGD DOOR DE

Free format text: TRANSFERIDO DE: NVI NEDERLANDS VACCININSTITUUT

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09R Decision of refusal: republication [chapter 9.2.3 patent gazette]

Free format text: REPUBLICACAO POR TER SIDO CONSTATADO ERRO MATERIAL.

B12B Appeal against refusal [chapter 12.2 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time