BRPI0604215A - método para projetar oligonucleotìdeos para técnicas de biologia molecular - Google Patents
método para projetar oligonucleotìdeos para técnicas de biologia molecularInfo
- Publication number
- BRPI0604215A BRPI0604215A BRPI0604215-5A BRPI0604215A BRPI0604215A BR PI0604215 A BRPI0604215 A BR PI0604215A BR PI0604215 A BRPI0604215 A BR PI0604215A BR PI0604215 A BRPI0604215 A BR PI0604215A
- Authority
- BR
- Brazil
- Prior art keywords
- oligonucleotides
- molecular biology
- designing
- sequences
- biology techniques
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 10
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 9
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 9
- 238000001823 molecular biology technique Methods 0.000 title abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biotechnology (AREA)
- Evolutionary Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
"MéTODO PARA PROJETAR OLIGONUCLEOTìDEOS PARA TéCNICAS DE BIOLOGIA MOLECULAR". A presente invenção revela um método que pode ser usado para identificar uma seqüência de DNA ou um grupo específico de seqüências de DNA de uma amostra biológica complexa. Métodos de biologia molecular diversos requerem o uso de seqüências curtas de DNA, denominadas oligonucleotídeos, que são sintetizados artificialmente a partir de uma descrição de suas bases de composição. O método revelado permite projetar oligonucleotídeos úteis para os procedimentos de biologia molecular, como procedimentos de projeto de sonda, e é caracterizado pela construção de uma base de dados das seqüências de referência, a seleção de um subconjunto de seqüências pertencendo aos organismos alvo, a seleção dos oligonucleotídeos candidatos de tais seqüências, a depuração desses oligonucleotídeos candidatos de acordo com critérios de especificidade de hibridização e estabilidade termodinâmica, e a classificação de tais oligonucleotídeos de acordo com sua especificidade taxonómica. Em um segundo aspecto, é revelado um método para projetar pares de oligonucleotídeos ou iniciadores, que são necessários em determinadas técnicas de biologia molecular, como técnicas de reação da cadeia da polimerase (PCR). Esse método é semelhante ao primeiro aspecto da invenção, porém são avaliados pares de oligonucleotídeos termodinamicamente compatíveis ou iniciadores que hibridizam à mesma seqüência a uma distância que está dentro de uma dada faixa.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2005002102 | 2005-08-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0604215A true BRPI0604215A (pt) | 2007-04-10 |
Family
ID=40636643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0604215-5A BRPI0604215A (pt) | 2005-08-17 | 2006-08-15 | método para projetar oligonucleotìdeos para técnicas de biologia molecular |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7853408B2 (pt) |
| AR (1) | AR054923A1 (pt) |
| AU (1) | AU2006203551B2 (pt) |
| BR (1) | BRPI0604215A (pt) |
| FR (1) | FR2889845A1 (pt) |
| MX (1) | MXPA06009317A (pt) |
| PE (1) | PE20070356A1 (pt) |
| ZA (1) | ZA200606828B (pt) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008119084A1 (en) * | 2007-03-28 | 2008-10-02 | The Children's Mercy Hospital | Method for identifying and selecting low copy nucleic acid segments |
| JP2012513194A (ja) | 2008-12-23 | 2012-06-14 | アストラゼネカ アクチボラグ | α５β１に向けられた標的結合剤およびその使用 |
| PT2363502T (pt) * | 2010-03-04 | 2017-05-17 | Miacom Diagnostics Gmbh | Fish multiplex melhorado |
| EP3498864B1 (en) * | 2011-03-10 | 2024-10-09 | Gen-Probe Incorporated | Methods for the selection and optimization of oligonucleotide tag sequences |
| DK3283524T3 (da) | 2015-04-17 | 2023-05-30 | Amgen Res Munich Gmbh | BISPECIFIKKE ANTISTOFKONSTRUKTIONER MOD CDH3 og CD3 |
| TWI744242B (zh) | 2015-07-31 | 2021-11-01 | 德商安美基研究（慕尼黑）公司 | Egfrviii及cd3抗體構築體 |
| TWI796283B (zh) | 2015-07-31 | 2023-03-21 | 德商安美基研究（慕尼黑）公司 | Ｍｓｌｎ及ｃｄ３抗體構築體 |
| TWI793062B (zh) | 2015-07-31 | 2023-02-21 | 德商安美基研究（慕尼黑）公司 | Ｄｌｌ３及ｃｄ３抗體構築體 |
| TWI829617B (zh) | 2015-07-31 | 2024-01-21 | 德商安美基研究（慕尼黑）公司 | Ｆｌｔ３及ｃｄ３抗體構築體 |
| SG11201805770UA (en) | 2016-02-03 | 2018-08-30 | Amgen Res Munich Gmbh | BCMA and CD3 Bispecific T Cell Engaging Antibody Constructs |
| CA3011942A1 (en) | 2016-02-03 | 2017-08-10 | Amgen Research (Munich) Gmbh | Psma and cd3 bispecific t cell engaging antibody constructs |
| AU2018214223B2 (en) | 2017-02-02 | 2025-08-14 | Amgen Inc. | Low pH pharmaceutical composition comprising T cell engaging antibody constructs |
| EP3592768B1 (en) | 2017-03-08 | 2024-12-25 | The Regents of The University of Michigan | Analyte detection |
| US20220148678A1 (en) * | 2019-02-28 | 2022-05-12 | Seegene, Inc. | Methods for determining a designable region of oligonucleotides |
| US12312412B2 (en) | 2020-05-19 | 2025-05-27 | Amgen Inc. | MAGEB2 binding constructs |
| CN116368154A (zh) | 2020-10-08 | 2023-06-30 | 阿菲姆德股份有限公司 | 三特异性结合剂 |
| AU2021374036A1 (en) | 2020-11-06 | 2023-06-08 | Amgen Inc. | Polypeptide constructs selectively binding to cldn6 and cd3 |
| KR20230098335A (ko) | 2020-11-06 | 2023-07-03 | 암젠 인크 | 클리핑 비율이 감소된 항원 결합 도메인 |
| MX2023005323A (es) | 2020-11-06 | 2023-05-19 | Amgen Inc | Construcciones polipeptidicas que se unen a cd3. |
| CA3200317A1 (en) | 2020-11-06 | 2022-05-12 | Amgen Inc. | Multitargeting bispecific antigen-binding molecules of increased selectivity |
| AR125290A1 (es) | 2021-04-02 | 2023-07-05 | Amgen Inc | Construcciones de unión a mageb2 |
| WO2022234102A1 (en) | 2021-05-06 | 2022-11-10 | Amgen Research (Munich) Gmbh | Cd20 and cd22 targeting antigen-binding molecules for use in proliferative diseases |
| AU2022320948A1 (en) | 2021-07-30 | 2024-01-18 | Affimed Gmbh | Duplexbodies |
| JP2024543822A (ja) | 2021-11-03 | 2024-11-26 | アフィメド ゲーエムベーハー | 二重特異性ｃｄ１６ａバインダー |
| AU2022381918A1 (en) | 2021-11-03 | 2024-06-13 | Affimed Gmbh | Bispecific cd16a binders |
| IL316597A (en) | 2022-05-12 | 2024-12-01 | Amgen Res Munich Gmbh | Multi-chain, multi-purpose, bispecific antigen-binding compounds with enhanced sensitivity |
| WO2024015860A2 (en) * | 2022-07-15 | 2024-01-18 | The Regents Of The University Of Michigan | Analyte detection using fluorogenic probes or multiplex technologies |
| JP2025533434A (ja) | 2022-09-14 | 2025-10-07 | アムジエン・インコーポレーテツド | 二重特異性分子安定化組成物 |
| MX2025014635A (es) | 2023-06-14 | 2026-02-03 | Amgen Inc | Moleculas captadoras de enmascaramiento de celulas t |
| CN119920326B (zh) * | 2025-04-07 | 2025-07-08 | 北京微岩医学检验实验室有限公司 | 一种引物集确定方法及装置 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030097223A1 (en) | 1999-12-14 | 2003-05-22 | Hitachi, Ltd. | Primer design system |
-
2006
- 2006-08-15 BR BRPI0604215-5A patent/BRPI0604215A/pt not_active IP Right Cessation
- 2006-08-16 PE PE2006000995A patent/PE20070356A1/es not_active Application Discontinuation
- 2006-08-16 MX MXPA06009317A patent/MXPA06009317A/es active IP Right Grant
- 2006-08-16 ZA ZA200606828A patent/ZA200606828B/xx unknown
- 2006-08-16 AR ARP060103582A patent/AR054923A1/es active IP Right Grant
- 2006-08-17 AU AU2006203551A patent/AU2006203551B2/en not_active Ceased
- 2006-08-17 US US11/506,089 patent/US7853408B2/en not_active Expired - Fee Related
- 2006-08-17 FR FR0607359A patent/FR2889845A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| PE20070356A1 (es) | 2007-04-05 |
| AU2006203551A1 (en) | 2007-03-08 |
| MXPA06009317A (es) | 2008-10-10 |
| AR054923A1 (es) | 2007-07-25 |
| US20070059743A1 (en) | 2007-03-15 |
| US7853408B2 (en) | 2010-12-14 |
| FR2889845A1 (fr) | 2007-02-23 |
| ZA200606828B (en) | 2008-03-26 |
| AU2006203551B2 (en) | 2011-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AR054923A1 (es) | Metodo para el diseno de oligonucleotidos para tecnicas de biologia molecular | |
| ES2429408T3 (es) | Examen genético fetal no invasivo mediante análisis digital | |
| ES2994737T3 (en) | Polymerase chain reaction primers and probes for mycobacterium tuberculosis | |
| JP7645315B2 (ja) | 保存された遺伝子座に基づく、哺乳動物に関するｄｎａメチル化測定 | |
| JP2007525998A (ja) | 脆弱ｘ症候群などのｓｔｒｐの検出 | |
| KR101301255B1 (ko) | 탄화플루오르옥탄술폰산 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법 | |
| KR101398548B1 (ko) | 저급 지방족 포화 알데하이드류 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법 | |
| US20040014101A1 (en) | Separating and/or identifying polymorphic nucleic acids using universal bases | |
| KR100957055B1 (ko) | 트리클로로에틸렌 노출 여부 확인용 바이오마커 및 이를이용한 확인 방법 | |
| JP4972104B2 (ja) | 病原体の同定のためのオリゴヌクレオチドマイクロアレイ | |
| US10927405B2 (en) | Molecular tag attachment and transfer | |
| RU2341795C2 (ru) | Гены, связанные с остеоартритом собак, и относящиеся к этому способы и композиции | |
| Zhang et al. | Oligonucleotide microarray for HLA‐DRB1 genotyping: preparation and clinical evaluation | |
| KR101301254B1 (ko) | 퍼플루오로옥탄산염 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법 | |
| EP1964929A1 (en) | Hla-b genotyping method and kit based on real-time pcr | |
| CN109868320A (zh) | 用于检测食道癌的组合物及其用途 | |
| JP2023536385A (ja) | コロナウイルスを含む呼吸器ウイルスを検出する構成物および方法 | |
| JP2022076117A (ja) | 前立腺がんを検査する方法 | |
| KR101383653B1 (ko) | 프로피온알데히드 특이적 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법 | |
| RU2445369C2 (ru) | СПОСОБ ОПРЕДЕЛЕНИЯ ГЕНОТИПА ЧЕЛОВЕКА ПО ПОЛИМОРФИЗМУ В ПОЗИЦИИ 185 ГЕНА BRCA1 (185delAG) | |
| WO2005059174A1 (es) | Método y kit para genotipificación de hla-drb basados en la pcr en tiempo real | |
| RU2445368C2 (ru) | СПОСОБ ОПРЕДЕЛЕНИЯ ГЕНОТИПА ЧЕЛОВЕКА ПО ПОЛИМОРФИЗМУ В ПОЗИЦИИ 5382 ГЕНА BRCA1 (5382insC) | |
| Noh et al. | Method for the differentiation of nine species of Sebastes with fluorescence melting curve analysis and dual-labeled probes | |
| Poerner et al. | 182-P: Evaluation of LABType® SSO HLA typing tests efficiency in a Brazilian bone marrow volunteer donor immunogenetic laboratory | |
| KR101079250B1 (ko) | 카바릴 노출 여부 확인용 바이오마커 및 이를 이용한 확인 방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2160 DE 29/05/2012. |