BRPI0604215A - método para projetar oligonucleotìdeos para técnicas de biologia molecular - Google Patents
método para projetar oligonucleotìdeos para técnicas de biologia molecularInfo
- Publication number
- BRPI0604215A BRPI0604215A BRPI0604215-5A BRPI0604215A BRPI0604215A BR PI0604215 A BRPI0604215 A BR PI0604215A BR PI0604215 A BRPI0604215 A BR PI0604215A BR PI0604215 A BRPI0604215 A BR PI0604215A
- Authority
- BR
- Brazil
- Prior art keywords
- oligonucleotides
- molecular biology
- designing
- sequences
- biology techniques
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 10
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 9
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 9
- 238000001823 molecular biology technique Methods 0.000 title abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biotechnology (AREA)
- Evolutionary Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
"MéTODO PARA PROJETAR OLIGONUCLEOTìDEOS PARA TéCNICAS DE BIOLOGIA MOLECULAR". A presente invenção revela um método que pode ser usado para identificar uma seqüência de DNA ou um grupo específico de seqüências de DNA de uma amostra biológica complexa. Métodos de biologia molecular diversos requerem o uso de seqüências curtas de DNA, denominadas oligonucleotídeos, que são sintetizados artificialmente a partir de uma descrição de suas bases de composição. O método revelado permite projetar oligonucleotídeos úteis para os procedimentos de biologia molecular, como procedimentos de projeto de sonda, e é caracterizado pela construção de uma base de dados das seqüências de referência, a seleção de um subconjunto de seqüências pertencendo aos organismos alvo, a seleção dos oligonucleotídeos candidatos de tais seqüências, a depuração desses oligonucleotídeos candidatos de acordo com critérios de especificidade de hibridização e estabilidade termodinâmica, e a classificação de tais oligonucleotídeos de acordo com sua especificidade taxonómica. Em um segundo aspecto, é revelado um método para projetar pares de oligonucleotídeos ou iniciadores, que são necessários em determinadas técnicas de biologia molecular, como técnicas de reação da cadeia da polimerase (PCR). Esse método é semelhante ao primeiro aspecto da invenção, porém são avaliados pares de oligonucleotídeos termodinamicamente compatíveis ou iniciadores que hibridizam à mesma seqüência a uma distância que está dentro de uma dada faixa.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CL2005002102 | 2005-08-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0604215A true BRPI0604215A (pt) | 2007-04-10 |
Family
ID=40636643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0604215-5A BRPI0604215A (pt) | 2005-08-17 | 2006-08-15 | método para projetar oligonucleotìdeos para técnicas de biologia molecular |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7853408B2 (pt) |
| AR (1) | AR054923A1 (pt) |
| AU (1) | AU2006203551B2 (pt) |
| BR (1) | BRPI0604215A (pt) |
| FR (1) | FR2889845A1 (pt) |
| MX (1) | MXPA06009317A (pt) |
| PE (1) | PE20070356A1 (pt) |
| ZA (1) | ZA200606828B (pt) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008119084A1 (en) * | 2007-03-28 | 2008-10-02 | The Children's Mercy Hospital | Method for identifying and selecting low copy nucleic acid segments |
| CA2748158A1 (en) | 2008-12-23 | 2010-07-01 | Astrazeneca Ab | Targeted binding agents directed to .alpha.5.beta.1 and uses thereof |
| ES2623859T3 (es) * | 2010-03-04 | 2017-07-12 | Miacom Diagnostics Gmbh | FISH múltiple mejorada |
| EP2683833B1 (en) | 2011-03-10 | 2018-09-26 | Gen-Probe Incorporated | Methods for the selection and optimization of oligonucleotide tag sequences |
| CN107750255B (zh) | 2015-04-17 | 2022-08-30 | 安进研发（慕尼黑）股份有限公司 | 用于cdh3和cd3的双特异性抗体构建体 |
| TWI829617B (zh) | 2015-07-31 | 2024-01-21 | 德商安美基研究（慕尼黑）公司 | Ｆｌｔ３及ｃｄ３抗體構築體 |
| TWI793062B (zh) | 2015-07-31 | 2023-02-21 | 德商安美基研究（慕尼黑）公司 | Ｄｌｌ３及ｃｄ３抗體構築體 |
| TWI744242B (zh) | 2015-07-31 | 2021-11-01 | 德商安美基研究（慕尼黑）公司 | Egfrviii及cd3抗體構築體 |
| TWI796283B (zh) | 2015-07-31 | 2023-03-21 | 德商安美基研究（慕尼黑）公司 | Ｍｓｌｎ及ｃｄ３抗體構築體 |
| IL313507A (en) | 2016-02-03 | 2024-08-01 | Amgen Res Munich Gmbh | Constructs of bispecific antibodies to BCMA and CD3 that bind to T cells, preparations containing the same and uses thereof |
| EA201891753A1 (ru) | 2016-02-03 | 2019-01-31 | Эмджен Рисерч (Мюник) Гмбх | Биспецифические конструкции антител к psma и cd3, вовлекающие т-клетки |
| SMT202500367T1 (it) | 2017-02-02 | 2025-11-10 | Amgen Res Munich Gmbh | Composizione farmaceutica a basso ph comprendente costrutti di anticorpo che reclutano le cellule t |
| WO2018165309A1 (en) | 2017-03-08 | 2018-09-13 | The Regents Of The University Of Michigan | Analyte detection |
| US20220148678A1 (en) * | 2019-02-28 | 2022-05-12 | Seegene, Inc. | Methods for determining a designable region of oligonucleotides |
| CA3183756A1 (en) | 2020-05-19 | 2021-11-25 | Amgen Inc. | Mageb2 binding constructs |
| CN116368154A (zh) | 2020-10-08 | 2023-06-30 | 阿菲姆德股份有限公司 | 三特异性结合剂 |
| CR20230229A (es) | 2020-11-06 | 2023-09-05 | Amgen Res Munich Gmbh | Moléculas de unión a antígeno biespecíficas con múltiples dianas de selectividad aumentada |
| UY39507A (es) | 2020-11-06 | 2022-03-31 | Amgen Res Munich Gmbh | Construcciones polipeptídicas que se unen selectivamente a cldn6 y cd3 |
| WO2022096704A1 (en) | 2020-11-06 | 2022-05-12 | Amgen Inc. | Antigen binding domain with reduced clipping rate |
| TW202225188A (zh) | 2020-11-06 | 2022-07-01 | 德商安美基研究（慕尼黑）公司 | 與ｃｄ３結合的多肽構建體 |
| US20250282887A1 (en) | 2021-04-02 | 2025-09-11 | Amgen Inc. | Mageb2 binding constructs |
| JP2024518369A (ja) | 2021-05-06 | 2024-05-01 | アムジェン リサーチ （ミュニック） ゲゼルシャフト ミット ベシュレンクテル ハフツング | 増殖性疾患で使用されるｃｄ２０及びｃｄ２２標的化抗原結合分子 |
| CA3216098A1 (en) | 2021-07-30 | 2023-02-02 | Uwe Reusch | Duplexbodies |
| US20260070986A1 (en) | 2021-11-03 | 2026-03-12 | Affimed Gmbh | Bispecific cd16a binders |
| IL312515A (en) | 2021-11-03 | 2024-07-01 | Affimed Gmbh | Bispecific cd16a binders |
| EP4522651A1 (en) | 2022-05-12 | 2025-03-19 | Amgen Research (Munich) GmbH | Multichain multitargeting bispecific antigen-binding molecules of increased selectivity |
| WO2024015860A2 (en) * | 2022-07-15 | 2024-01-18 | The Regents Of The University Of Michigan | Analyte detection using fluorogenic probes or multiplex technologies |
| TW202421650A (zh) | 2022-09-14 | 2024-06-01 | 美商安進公司 | 雙特異性分子穩定組成物 |
| UY40797A (es) | 2023-06-14 | 2024-12-31 | Amgen Inc | Moléculas captadoras de enmascaramiento de células t |
| CN119920326B (zh) * | 2025-04-07 | 2025-07-08 | 北京微岩医学检验实验室有限公司 | 一种引物集确定方法及装置 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030097223A1 (en) | 1999-12-14 | 2003-05-22 | Hitachi, Ltd. | Primer design system |
-
2006
- 2006-08-15 BR BRPI0604215-5A patent/BRPI0604215A/pt not_active IP Right Cessation
- 2006-08-16 ZA ZA200606828A patent/ZA200606828B/xx unknown
- 2006-08-16 MX MXPA06009317A patent/MXPA06009317A/es active IP Right Grant
- 2006-08-16 AR ARP060103582A patent/AR054923A1/es active IP Right Grant
- 2006-08-16 PE PE2006000995A patent/PE20070356A1/es not_active Application Discontinuation
- 2006-08-17 US US11/506,089 patent/US7853408B2/en not_active Expired - Fee Related
- 2006-08-17 FR FR0607359A patent/FR2889845A1/fr not_active Withdrawn
- 2006-08-17 AU AU2006203551A patent/AU2006203551B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| MXPA06009317A (es) | 2008-10-10 |
| PE20070356A1 (es) | 2007-04-05 |
| AU2006203551B2 (en) | 2011-06-02 |
| FR2889845A1 (fr) | 2007-02-23 |
| AU2006203551A1 (en) | 2007-03-08 |
| US20070059743A1 (en) | 2007-03-15 |
| US7853408B2 (en) | 2010-12-14 |
| ZA200606828B (en) | 2008-03-26 |
| AR054923A1 (es) | 2007-07-25 |
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Legal Events
| Date | Code | Title | Description |
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| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A ANUIDADE. |
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| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2160 DE 29/05/2012. |