BRPI0607194A2 - método para analisar quantitativamente um microorganismo por marcação de rrna - Google Patents

método para analisar quantitativamente um microorganismo por marcação de rrna

Info

Publication number
BRPI0607194A2
BRPI0607194A2 BRPI0607194-5A BRPI0607194A BRPI0607194A2 BR PI0607194 A2 BRPI0607194 A2 BR PI0607194A2 BR PI0607194 A BRPI0607194 A BR PI0607194A BR PI0607194 A2 BRPI0607194 A2 BR PI0607194A2
Authority
BR
Brazil
Prior art keywords
microorganism
quantitatively analyzing
rna labeling
quantifying
rrna
Prior art date
Application number
BRPI0607194-5A
Other languages
English (en)
Inventor
Kazunori Matsuda
Takashi Asahara
Koji Nomoto
Mayumi Kiwaki
Hirokazu Tsuji
Original Assignee
Yakult Honsha Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yakult Honsha Kk filed Critical Yakult Honsha Kk
Publication of BRPI0607194A2 publication Critical patent/BRPI0607194A2/pt
Publication of BRPI0607194B1 publication Critical patent/BRPI0607194B1/pt
Publication of BRPI0607194B8 publication Critical patent/BRPI0607194B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MéTODO PARA ANALISAR QUANTITATIVAMENTE UM MICROORGANISMO POR MARCAçAO DE rRNA. Tem-se por objetivo proporcionar um método para quantificar/detectar um microorganismo, por meio do que um microorganismo em um estado viável pode ser altamente e mais adequadamente detectado. Isto é, um método para quantificar um microorganismo alvo usando-se a quantidade de um rRNA do microorganismo alvo em uma amostra de teste como uma indicação.
BRPI0607194A 2005-01-31 2006-01-30 método e kit para analisar quantitativamente um microrganismo por marcação de rrna BRPI0607194B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2005023448 2005-01-31
JP2005-023448 2005-01-31
PCT/JP2006/301467 WO2006080501A1 (ja) 2005-01-31 2006-01-30 ｒＲＮＡを標的とした微生物の定量的解析方法

Publications (3)

Publication Number Publication Date
BRPI0607194A2 true BRPI0607194A2 (pt) 2009-08-25
BRPI0607194B1 BRPI0607194B1 (pt) 2018-02-14
BRPI0607194B8 BRPI0607194B8 (pt) 2021-07-27

Family

ID=36740518

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0607194A BRPI0607194B8 (pt) 2005-01-31 2006-01-30 método e kit para analisar quantitativamente um microrganismo por marcação de rrna

Country Status (15)

Country Link
US (1) US10174386B2 (pt)
EP (1) EP1845158B1 (pt)
JP (1) JP5238248B2 (pt)
KR (1) KR101409193B1 (pt)
CN (3) CN101111596A (pt)
AU (1) AU2006209416B2 (pt)
BR (1) BRPI0607194B8 (pt)
CA (1) CA2596059C (pt)
DK (1) DK1845158T3 (pt)
ES (1) ES2428146T3 (pt)
NO (1) NO342747B1 (pt)
NZ (1) NZ560246A (pt)
PL (1) PL1845158T3 (pt)
RU (1) RU2420595C2 (pt)
WO (1) WO2006080501A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2909099B1 (fr) * 2006-11-24 2012-10-19 Univ Aix Marseille Ii Methode de diagnostic et de suivi d'une vaginose bacterienne par quantification moleculaire.
EP2155909B1 (en) 2007-06-01 2018-03-07 Council Of Scientific & Industrial Research Method for simultaneous detection and discrimination of bacterial, fungal, parasitic and viral infections of eye and central nervous system
CN101240315A (zh) * 2008-02-21 2008-08-13 上海交通大学 检测药物防癌效果的非损伤性分子方法
CN102094089B (zh) * 2010-12-06 2013-09-11 北京大北农科技集团股份有限公司 用于鉴定短小乳杆菌的引物序列及其应用
CN102936619B (zh) * 2012-06-15 2014-10-22 清华大学 定量检测大肠杆菌rna的方法及其专用标准品和应用
RU2530170C1 (ru) * 2013-03-27 2014-10-10 Михаил Аркадьевич Шурдов Способ детекции стволовых раковых клеток
JP6383552B2 (ja) * 2014-03-27 2018-08-29 三菱重工業株式会社 スフィンゴモナス属細菌の検出方法及びそのプライマー、生物処理槽の活性予測方法
CN106755470A (zh) * 2017-01-16 2017-05-31 吉林省浦生泰生物技术有限责任公司 一种利用q‑pcr检测混合益生菌中益生菌种类和含量的方法
JP6883435B2 (ja) * 2017-01-30 2021-06-09 株式会社Kri 活性汚泥の診断方法、活性汚泥処理装置の最適運転条件決定方法、活性汚泥異常要因微生物の同定方法及び活性汚泥処理装置の運転改善方法
JP7209930B2 (ja) * 2017-05-18 2023-01-23 株式会社ヤクルト本社 パーキンソン病の判定マーカーおよび判定方法
PT3868929T (pt) 2018-01-15 2025-07-28 Chemiefaser Lenzing Ag Corpo moldado que apresenta elastano incorporado em celulose e processo de produção do mesmo
CN109402288A (zh) * 2018-11-29 2019-03-01 安徽省农业科学院烟草研究所 一种用于检测根串珠霉菌的引物及检测方法
WO2020121908A1 (ja) * 2018-12-13 2020-06-18 株式会社ヤクルト本社 便の硬さを測定する方法およびこれを利用した便の状態の評価方法
CN110029155A (zh) * 2019-05-27 2019-07-19 天益健康科学研究院（镇江）有限公司 一种基于荧光定量pcr组合式肠道细菌检测方法
CN110512010B (zh) * 2019-08-20 2023-04-07 吉林农业大学 一种检测大肠杆菌rRNA转录速率的试剂盒及方法
CN112029885B (zh) * 2020-09-29 2022-06-07 广东省微生物研究所（广东省微生物分析检测中心） 用于鉴定瑞士乳杆菌、发酵乳杆菌和嗜酸乳杆菌的分子标记、检测引物和检测方法
CN113430287A (zh) * 2021-06-11 2021-09-24 中山大学肿瘤防治中心（中山大学附属肿瘤医院、中山大学肿瘤研究所） 一种肠道益生菌快速检测试剂盒及其应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1154410A (zh) * 1995-11-06 1997-07-16 麦克罗戴克公司 微生物定量和检测的方法及试剂盒
JPH10210980A (ja) 1997-01-29 1998-08-11 Asahi Breweries Ltd 乳酸菌検出用オリゴヌクレオチド及び該菌の検出方法
JPH10248600A (ja) 1997-03-10 1998-09-22 Sumitomo Electric Ind Ltd 癌細胞の検出方法及び癌細胞検出用プライマ
US6602663B1 (en) 1998-09-18 2003-08-05 Chugai Seiyaku Kabushiki Kaisha Method for detection or measuring plasmocytoma cells
US6894156B2 (en) * 1999-04-15 2005-05-17 E. I. Du Pont De Nemours And Company Nucleic acid fragments for the identification of dechlorinating bacteria
JP4724380B2 (ja) * 1999-04-20 2011-07-13 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
JP2001112485A (ja) 1999-10-19 2001-04-24 Yakult Honsha Co Ltd 腸内細菌用プライマー及び該プライマーを用いた検出方法
AUPQ909000A0 (en) * 2000-07-28 2000-08-24 University Of Sydney, The A method of detecting microorganisms
JP2002238585A (ja) 2001-02-21 2002-08-27 Joji Oshima 核酸定量を応用した細菌の定量法及び菌種判別法
DE60210621T2 (de) * 2001-07-19 2007-03-08 Infectio Diagnostic (I.D.I.) Inc., Sainte-Foy Universelle methode und zusammensetzung zur schnellen lysierung von zellen zur freisetzung von nukleinsäuren und ihre detektion
JP2003259879A (ja) 2002-03-12 2003-09-16 Mitsui Eng & Shipbuild Co Ltd 複合微生物群の定量的解析方法
EP1529117A2 (en) * 2002-08-08 2005-05-11 University of Lausanne Method for the rapid assessment of the presence and viability of bacterial cells and use thereof
US20060257871A1 (en) 2002-11-12 2006-11-16 Franck Chaubron One step real-time rt pcr kits for the universal detection of organisms in industrial products
JP4304976B2 (ja) * 2002-12-19 2009-07-29 東ソー株式会社 リボゾームｒｎａを標的とした抗酸菌の検出法
CN1232651C (zh) * 2003-01-21 2005-12-21 中国人民解放军军事医学科学院微生物流行病研究所 一种用于临床细菌检测的生物芯片
US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA

Also Published As

Publication number Publication date
AU2006209416B2 (en) 2011-02-10
NZ560246A (en) 2011-03-31
JPWO2006080501A1 (ja) 2008-06-19
BRPI0607194B1 (pt) 2018-02-14
NO342747B1 (no) 2018-08-06
US20090170078A1 (en) 2009-07-02
PL1845158T3 (pl) 2014-02-28
KR20070105980A (ko) 2007-10-31
CN110079617A (zh) 2019-08-02
EP1845158A1 (en) 2007-10-17
DK1845158T3 (da) 2013-11-11
CA2596059C (en) 2015-08-04
WO2006080501A1 (ja) 2006-08-03
US10174386B2 (en) 2019-01-08
EP1845158B1 (en) 2013-09-11
NO20073675L (no) 2007-08-28
CN104232769B (zh) 2020-04-14
ES2428146T3 (es) 2013-11-06
BRPI0607194B8 (pt) 2021-07-27
CA2596059A1 (en) 2006-08-03
KR101409193B1 (ko) 2014-06-19
RU2420595C2 (ru) 2011-06-10
CN104232769A (zh) 2014-12-24
EP1845158A4 (en) 2009-12-09
CN101111596A (zh) 2008-01-23
JP5238248B2 (ja) 2013-07-17
AU2006209416A1 (en) 2006-08-03
RU2007132732A (ru) 2009-03-10

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