BRPI0607661A2 - amplificação e clonagem de molécula de único dna usando amplificação de cìrculo rolante - Google Patents
amplificação e clonagem de molécula de único dna usando amplificação de cìrculo rolanteInfo
- Publication number
- BRPI0607661A2 BRPI0607661A2 BRPI0607661-0A BRPI0607661A BRPI0607661A2 BR PI0607661 A2 BRPI0607661 A2 BR PI0607661A2 BR PI0607661 A BRPI0607661 A BR PI0607661A BR PI0607661 A2 BRPI0607661 A2 BR PI0607661A2
- Authority
- BR
- Brazil
- Prior art keywords
- amplification
- cloning
- rolling circle
- dna molecule
- single dna
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 7
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 7
- 108020004414 DNA Proteins 0.000 title abstract 4
- 238000005096 rolling process Methods 0.000 title abstract 4
- 238000010367 cloning Methods 0.000 title abstract 3
- 102000053602 DNA Human genes 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 4
- 108020004638 Circular DNA Proteins 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000006073 displacement reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
AMPLIFICAçãO E CLONAGEM DE MOLéCULA DE úNICO DNA USANDO AMPLIFICAçãO DE CìRCULO ROLANTE. A presente invenção refere-se, por exemplo, a um método para amplificar um pequeno número de cópias (por exemplo, uma única cópia) de uma molécula de DNA circular de filamento único (por exemplo, tendo um tamanho de cerca de 5 a 6 kb) por um mecanismo de círculo rolante isotérmico, usando iniciadores aleatórios ou parcialmente aleatórios e uma DNA polimerase tipo-F29. Método, o qual também pode ser usado para amplificar DNAs por tipos não rolantes de amplificação de múltiplos deslocamentos, compreende incubar os componentes da reação em um pequeno volume, por exemplo, cerca de 10 <109>l ou menos, tal como cerca de 0,6 <109>d ou menos. O grau de amplificação pode ser cerca de 109 vezes, ou superior. é descrito um método para clonagem de DNA livre de células, usando o método de amplificação de círculo rolante da invenção.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67585005P | 2005-04-29 | 2005-04-29 | |
| US72207005P | 2005-09-30 | 2005-09-30 | |
| US72530005P | 2005-10-12 | 2005-10-12 | |
| PCT/US2006/016349 WO2006119066A2 (en) | 2005-04-29 | 2006-05-01 | Amplification and cloning of single dna molecules using rolling circle amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0607661A2 true BRPI0607661A2 (pt) | 2009-09-22 |
Family
ID=36962781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0607661-0A BRPI0607661A2 (pt) | 2005-04-29 | 2006-05-01 | amplificação e clonagem de molécula de único dna usando amplificação de cìrculo rolante |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8497069B2 (pt) |
| EP (1) | EP1885880B1 (pt) |
| CN (1) | CN101213311B (pt) |
| AT (1) | ATE474937T1 (pt) |
| AU (1) | AU2006242387B2 (pt) |
| BR (1) | BRPI0607661A2 (pt) |
| CA (1) | CA2607532C (pt) |
| DE (1) | DE602006015633D1 (pt) |
| DK (1) | DK1885880T3 (pt) |
| IL (1) | IL187002A (pt) |
| WO (1) | WO2006119066A2 (pt) |
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| US10093552B2 (en) | 2008-02-22 | 2018-10-09 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
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| GB0901593D0 (en) | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| CA2756463C (en) | 2009-03-24 | 2019-01-22 | University Of Chicago | Slip chip device and methods |
| US9447461B2 (en) * | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
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| EP2957641B1 (en) | 2009-10-15 | 2017-05-17 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| US20110195457A1 (en) * | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
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| EP2753715A4 (en) * | 2011-09-09 | 2015-05-20 | Univ Leland Stanford Junior | METHOD FOR OBTAINING A SEQUENCE |
| US9353393B2 (en) | 2012-02-15 | 2016-05-31 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| WO2014130796A1 (en) * | 2013-02-22 | 2014-08-28 | The Broad Institute, Inc. | Methods for assessing the risk of canine atopic dermatitis |
| CA2903874C (en) | 2013-03-15 | 2024-02-20 | Theranos, Inc. | Nucleic acid amplification |
| JP2016521120A (ja) | 2013-03-15 | 2016-07-21 | セラノス， インコーポレイテッド | 核酸増幅 |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| AU2014233145A1 (en) | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Nucleic acid amplification |
| CN103276074B (zh) * | 2013-05-21 | 2015-08-19 | 昆山彭济凯丰生物科技有限公司 | 一种合成短单链脱氧核糖核苷酸探针的方法 |
| TWI707038B (zh) | 2013-08-05 | 2020-10-11 | 美商扭轉生物科技有限公司 | 重新合成之基因庫 |
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| CN104695027B (zh) * | 2013-12-06 | 2017-10-20 | 中国科学院北京基因组研究所 | 测序文库及其制备和应用 |
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| EP4170044B1 (en) | 2014-06-06 | 2026-03-11 | Cornell University | Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions |
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| CN107250383B (zh) * | 2015-02-17 | 2022-09-06 | 深圳华大智造科技股份有限公司 | 使用受控的链置换的dna序列测定 |
| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| JP6698708B2 (ja) | 2015-06-09 | 2020-05-27 | ライフ テクノロジーズ コーポレーション | 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体 |
| KR20180050411A (ko) | 2015-09-18 | 2018-05-14 | 트위스트 바이오사이언스 코포레이션 | 올리고핵산 변이체 라이브러리 및 그의 합성 |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
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| EP3420110B1 (en) | 2016-02-26 | 2021-09-22 | The Board of Trustees of the Leland Stanford Junior University | Multiplexed single molecule rna visualization with a two-probe proximity ligation system |
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| CN110225979B (zh) * | 2017-05-23 | 2024-05-31 | 深圳华大基因股份有限公司 | 基于滚环扩增的基因组目标区域富集方法及其应用 |
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| AU2018328847A1 (en) | 2017-09-11 | 2020-04-23 | Twist Bioscience Corporation | GPCR binding proteins and synthesis thereof |
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| WO2024138550A1 (zh) * | 2022-12-29 | 2024-07-04 | 深圳华大智造科技股份有限公司 | 一种核酸扩增方法及其在测序中的用途 |
| CN116875671A (zh) * | 2023-07-18 | 2023-10-13 | 浙江大学 | 用于dna扩增并标记的标签发夹引物 |
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| RU2048522C1 (ru) * | 1992-10-14 | 1995-11-20 | Институт белка РАН | Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления |
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| US6210882B1 (en) * | 1998-01-29 | 2001-04-03 | Mayo Foundation For Medical Education And Reseach | Rapid thermocycling for sample analysis |
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| US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
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| CU23400A1 (es) | 2003-04-29 | 2009-08-04 | Ct Ingenieria Genetica Biotech | Método para la obtención de minicírculos replicativos de adn para transferencia génica o como inmunomoduladores |
| WO2005029041A2 (en) | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
-
2006
- 2006-05-01 AT AT06751837T patent/ATE474937T1/de not_active IP Right Cessation
- 2006-05-01 CN CN200680024024.4A patent/CN101213311B/zh not_active Expired - Lifetime
- 2006-05-01 DK DK06751837.3T patent/DK1885880T3/da active
- 2006-05-01 WO PCT/US2006/016349 patent/WO2006119066A2/en not_active Ceased
- 2006-05-01 EP EP06751837A patent/EP1885880B1/en not_active Expired - Lifetime
- 2006-05-01 US US11/919,515 patent/US8497069B2/en active Active
- 2006-05-01 DE DE602006015633T patent/DE602006015633D1/de not_active Expired - Lifetime
- 2006-05-01 CA CA2607532A patent/CA2607532C/en not_active Expired - Lifetime
- 2006-05-01 BR BRPI0607661-0A patent/BRPI0607661A2/pt active IP Right Grant
- 2006-05-01 AU AU2006242387A patent/AU2006242387B2/en not_active Expired
-
2007
- 2007-10-29 IL IL187002A patent/IL187002A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| EP1885880A2 (en) | 2008-02-13 |
| IL187002A0 (en) | 2008-02-09 |
| EP1885880B1 (en) | 2010-07-21 |
| CA2607532C (en) | 2013-03-12 |
| WO2006119066A2 (en) | 2006-11-09 |
| HK1112490A1 (en) | 2008-09-05 |
| IL187002A (en) | 2012-03-29 |
| US20090176280A1 (en) | 2009-07-09 |
| DK1885880T3 (da) | 2010-11-08 |
| CN101213311A (zh) | 2008-07-02 |
| CN101213311B (zh) | 2013-02-13 |
| CA2607532A1 (en) | 2006-11-09 |
| AU2006242387B2 (en) | 2011-01-06 |
| US8497069B2 (en) | 2013-07-30 |
| WO2006119066A3 (en) | 2007-03-15 |
| ATE474937T1 (de) | 2010-08-15 |
| DE602006015633D1 (de) | 2010-09-02 |
| AU2006242387A1 (en) | 2006-11-09 |
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